Conserved Protein Domain Family
APC10-HECTD3

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cd08666: APC10-HECTD3 
APC10-like DOC1 domain of HECTD3, a HECT E3 ubiquitin ligase protein that mediates substrate ubiquitination.
This model represents the APC10/DOC1 domain present in HECTD3, a HECT (Homologous to the E6-AP Carboxyl Terminus) E3 ubiquitin ligase protein. HECT E3 ubiquitin ligases mediate substrate ubiquitination (or ubiquitylation), and are a component of the ubiquitin-26S proteasome pathway for selective proteolytic degradation. They also regulate the trafficking of many receptors, channels, transporters and viral proteins. HECTD3 (HECT domain-containing protein3) contains a C-terminal HECT domain with the active site for ubiquitin transfer onto substrates, and an N-terminal APC10/DOC1 domain, which is responsible for substrate recognition and binding. HECTD3 specifically recognizes the Trio-binding protein, Tara (Trio-associated repeat on actin), implicated in regulating actin cytoskeletal, cell motility and cell growth. Tara also binds to TRF1 and may participate in telomere maintenance and/or mitotic regulation through interacting with TRF1. HECTD3 interacts with and promotes the ubiquitination of Syntaxin 8, an endosomal syntaxin proposed to mediate distinct steps of endosomal protein trafficking. HECTD3-mediated Syntaxin 8 degradation has been suggested to contribute to the pathophysiology of neurodegenerative diseases.
Statistics
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PSSM-Id: 176487
View PSSM: cd08666
Aligned: 9 rows
Threshold Bit Score: 235.382
Threshold Setting Gi: 221110686
Created: 4-Mar-2010
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
putative ligand
Feature 1:putative ligand binding site [chemical binding site]
Evidence:
  • Comment:based on sequence homology to the protein structure of APC10 subunit of the human anaphase-promoting complex.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                          #                           #     # #         
NP_078878    238 GSVKQYvESIDVSSYTee---fNVSCLTDSNADTYWESDGSQCQHWVRLTMKKGTIVKKLLLTVDttDDNFMPKRVVVYG 314 human
XP_422429    222 GSVKQYvDSIDVSSYTed---fNVSCLTDSHADTYWESDGSQGQHWVRLNMKKGTIVKKLLLTVDttDENFMPKRVAVYG 298 chicken
NP_001086963 221 GSVKQYvESISVSSATve---nNLSCLTDGYHDTYWESDGSDGQHWIQLNMKEGTVVKKLVLTVDstDETYMPKGIAVYG 297 African clawed ...
AAI24338     231 GSVKQCvTSIDVSSSSedp-sgGASCLTDGDTETYWESDGMQGQHWIRLHMRRGTVVNKLILTVDstDDNYMPKRVTVYG 309 zebrafish
XP_002611055 221 GSVKQYvESLEVSSYRde---dNSDCLTDGDTETYWESDGSQGQHWIRLKMKRSTIVKKLMIGVEasDDNYTPNRIVVMG 297 Florida lancelet
XP_002114258  62 GNLKDYvESIAVSSMEe-----DKDALTDGNQSSYWESDGSQGCHWIRLRIKKGVIIKCLLVTVDpnDDNYMPHLIEVKG 136 Trichoplax adha...
XP_002154828  55 GGASRYvESITVSSEQd-----SANNLLDNDPDTYWESDGSSGRHHIRLQIKAGVIIQKLIIGVDdsDDNYMPSRVLVYC 129 green hydra
XP_001634647  67 GSISHYvDGIEVSSVIgkledsSKDCLTDGDPVTYWESDGNQGRHWVRLSIKPGVIIKRLYIELDarDDNYMPYRIIISG 146 starlet sea ane...
XP_422428    216 DCVKDHlNGIEVSSQLdd---fKVSHLTDNQGDTYWESSGSPGQHWVRLHMKKGSVIKKLWLMLNanINSYVPRRVAVYG 292 chicken
Feature 1                                                          #       
NP_078878    315 Ge-gDNLKKLSDVSIDEtliGDVCVLEDMTvHLPIIEIRIVECRDDGIDVRLRGVKIK 371 human
XP_422429    299 Ge-gDNLKKLNDVGIDEsyiGDVCVLEDMTtHLPVIEIRIVECRDDGIDVRLRGIKIK 355 chicken
NP_001086963 298 Ge-rDALKKINDVSIDEnliGDVCILEDMTtHLPIIEIRISQCLDEGIDVRIRGLKIK 354 African clawed frog
AAI24338     310 Ge-gDNLKKYSDVTIDDtliGEVCVLEDMTsHLPVIEVRIEECRDEGIDVRIRGLKIK 366 zebrafish
XP_002611055 298 Ge-lDSMVKLNDISVNDgfsGDLTVLENMTqHYPYIEIRIKDCKDDGIDTRIHGLKIK 354 Florida lancelet
XP_002114258 137 Gd-hDDMRKMAEVLMDCtkmSDVTILQDMAeYLPEIEIRIKECKDEGIDTRIHGIKII 193 Trichoplax adhaerens
XP_002154828 130 LtdsVQSKLLNEVRIDGcqnGDVVILENAAeYYPQIEIRIKECKDEGIDCRIHYIKIV 187 green hydra
XP_001634647 147 Ge-vGHFKQLNEVFVDLtvtGDVLVLEEMTqYFHIIEIKIKECKDDGIDTRIHGIKIV 203 starlet sea anemone
XP_422428    293 Gp-aNRLQHLRTVLINEnsyQDVCILRDMKtHVPVLEIRILECREQGYNVRLRGIKFK 349 chicken

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