Conserved Protein Domain Family
PI-PLCXDc_CG14945_like

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cd08622: PI-PLCXDc_CG14945_like 
Catalytic domain of Drosophila melanogaster CG14945-like proteins similar to phosphatidylinositol-specific phospholipase C, X domain containing
This subfamily corresponds to the catalytic domain present in uncharacterized metazoan Drosophila melanogaster CG14945-like proteins, which are similar to eukaryotic phosphatidylinositol-specific phospholipase C, X domain containing proteins (PI-PLCXD). The typical eukaryotic phosphoinositide-specific phospholipase C (PI-PLC, EC 3.1.4.11) has a multidomain organization that consists of a PLC catalytic core domain, and various regulatory domains. The catalytic core domain is assembled from two highly conserved X- and Y-regions split by a divergent linker sequence. In contrast, eukaryotic PI-PLCXDs contain a single TIM-barrel type catalytic domain, X domain, and are more closely related to bacterial PI-PLCs, which participate in Ca2+-independent PI metabolism, hydrolyzing the membrane lipid phosphatidylinositol (PI) to produce phosphorylated myo-inositol and diacylglycerol (DAG). Although the biological function of eukaryotic PI-PLCXDs still remains unclear, it may distinct from that of typical eukaryotic PI-PLCs.
Statistics
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PSSM-Id: 176559
View PSSM: cd08622
Aligned: 10 rows
Threshold Bit Score: 337.378
Threshold Setting Gi: 196006928
Created: 8-Mar-2010
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
catalytic siteputative active
Feature 1:catalytic site [active site]
Evidence:
  • Comment:Both prokaryotic and eukaryotic PI-PLCs utilize a similar catalytic mechanism, a general base and acid catalysis involving two well conserved histidines. It consists of two steps, a phosphotransfer and a phosphodiesterase reaction.
  • Comment:Based on structure evidence of the catalytic site in Bacillus cereus phosphatidylinositol-specific phospholipase C.
  • Citation:PMID 2509427
  • Citation:PMID 8386017
  • Citation:PMID 7664726

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                         #                                                              
NP_609541    202 KSKIGEMRLRDLFIPGTHDSGSYRPNfdpllresLVTKYALTQDDDIRGQLMHGVRYLDIRVGYYrns------------ 269 fruit fly
ACE75384     150 RRQLGRLPIGSLMIPGTHNSGCYKHEdlt--rkdAFQKYLLTQDRDVWTQLVHGIRYLDIRVGHYpppmhngshsaddan 227 Glyptapanteles ...
EEZ98658     496 RMQIGTQKIGNLFIPGTHNSGAYVGVp------kILENYILNQDRSIWTQLVHGIRYLDLRIGYYen------------- 556 red flour beetle
XP_001658539  57 RSHVGQFRMRELFIPGTHDSASYKKSlny--qenIVTKYSLTQEDDIRKQLLQGVRYIDLRVGYYqvs------------ 122 yellow fever mo...
XP_001122651  97 KDILGPLRVKDIFLPGTHDSAAYDVNgti---isIISKFAVTQDLDILGQLIHGVRYLDIRLGHYpdn------------ 161 honey bee
XP_001815277 124 IQPAWMSKIGDMMIPGTHNSGAWRTKl------pGFKNYVLNQDKSMWEQLVYGIRYFDIRVGRYgds------------ 185 red flour beetle
XP_002113330 296 KDSIGNVRIKDLTIPGTHNSACYNMTv------pLADALTQSQSETFEQQLFDGIRYFDLHVEYYgky------------ 357 Trichoplax adha...
XP_001843372 116 EKSIGNVSLKDLFLPGTHCSACYQTKana--nnnLLKKVGFHQDLDIWTQLVFGVRYLDFSIGYHpyhn----------- 182 southern house ...
XP_002433767 118 CQKLGHLGFSQLFIPGTHNSAMYDVNspe--svsLVDHFLFNQEEPIINQLYYGIRSLDLRVQEKr-------------- 181 black-legged tick
NP_648828    148 IKDINRMALKDIFLPGTHASAAVLGSssks-nsiLVRDYLVAQQLDVWSQLVFGIRYLDLSIGYKnmnte---------- 216 fruit fly
Feature 1                 #                                                                      
NP_609541    270 --pdPFFIYHGITKq-rPLQEVINQVRDFVYET-NEIIIFGLKEFPVGFgkg----lgvHRLLISYLRDQLKDLIAhps- 340 fruit fly
ACE75384     228 htsgRFWVNHDVIRi-nPLATIIRDVRNFLDVArGEVVILDFHRFPVGFesr----psrHRRLATMLYREFGGLILkpsr 302 Glyptapanteles ...
EEZ98658     557 ---dGFYVNHDLVRi-tKVVQIFKEIRKFVQLApKEIIVVDFHRFPYPSnfn----stlHEKFVSLVYDYLGDLALppgg 628 red flour beetle
XP_001658539 123 --npQFWANHGISRl-hPLKQILHQIRDYALET-NEIIIVDVQEFPVGFgkq----yevHDKLIQFLNETLHDVMAdps- 193 yellow fever mo...
XP_001122651 162 --seIWWTIHGPFYrsvPLKTVADHVKNFLDNT-EEIVIMDIREFAVGFnny-----sdHLALVSHLEEEFRDYYLrrdn 233 honey bee
XP_001815277 186 --pqSLYINHDFVKc-tELVPELQSVAKFLQKSpKEVVVLDFHRFPHPKdfl----eghHLELLRVVRDIFGDLIYpysr 258 red flour beetle
XP_002113330 358 --edKFWFTHHEWStevSVSHFLNLIKNFITNN-QEIVMLDFNQFYYFNhp------saHDELIELIIKHLGDDMAlvs- 427 Trichoplax adha...
XP_001843372 183 -strNFWVMNEGYRv-gWILPILHDIRRFVILS-EETIVLDIRRFPLGFhsh----peqHVEFLSILDQELGDLAYrrpy 255 southern house ...
XP_002433767 182 ---gEFWITHDLVRgqvTVREVLQQVRQFVEAT-GEPVLLDFHRFTTGFssknnnsvenHKKLVTLITQELGDFLLerd- 256 black-legged tick
NP_648828    217 ndadNFWIANENMLi-tPLLTVLRDVRQFVKRS-GEVVVLDFSSFPIGFykh----peiYSSLFRLIRQELGDETYrrnv 290 fruit fly
Feature 1                                                                                        
NP_609541    341 ----ltwGASLRDIWa----------rRQNVFLAYDHEamvee--fpdvLFGSVEQRWGNkqTWDQLETYLRNVNDFdvs 404 fruit fly
ACE75384     303 g--vdglGPTLNEIWn----------sGRRLIICYGDKhtvn---eyewLWPPLTQVWGNqqTAQGLFEYLNSVLMGper 367 Glyptapanteles ...
EEZ98658     629 l--qvgkGPTLNEIWa----------qNKNVIICYADKaiar---enywLWQPLYQHWANtkNVDTLKSFLSRAIKEhrv 693 red flour beetle
XP_001658539 194 ----itwYGRLSEIWa----------rNKTVILCYDHEyamrs--ypdvVWRSAEQKWGDvqSLEELKSFLFKIHTRyly 257 yellow fever mo...
XP_001122651 234 ---gqgwGITLNEIWs----------sGKRLIIGYEDSkvva---nhasVWPCVTHKWGNvkTVEDLYKYLHKIETNnrd 297 honey bee
XP_001815277 259 f--mhpkGPKLREFWq----------sQKRVIISYRQDyfir---rnpwLWPGVQRDWGNvqKLQNLENFVKTKISKpvr 323 red flour beetle
XP_002113330 428 ----ysqDVTVGKLWe----------eNKRVIVAYDGKlqsenyrnenrLWPTIQTEWDSveTLDDVKKNLDQEINQrh- 492 Trichoplax adha...
XP_001843372 256 f-dnaddDQTVSYHLtied----mrrqGKYILITYNHAasln---dsdlIWAPWTKYSSSslKHTELSQFMNKLFSQkh- 326 southern house ...
XP_002433767 257 -----gtAVKLADILgqcqpvnrgarrRRTVIVCYNAPyfgq---gsyyLSPGVRQLWANavNLTSLRSYLENRVCEpsf 328 black-legged tick
NP_648828    291 gkdencaNRNFSEILlq---------kRHLVILFPTQElpypd-resnmLCPPWQRFSTSfmNISQTLDYMRLLFSRkp- 359 fruit fly
Feature 1                                                                                        
NP_609541    405 r---fssRPVSDMAELTPEtwdvil----dktgglRKMADNVNWRISQLYRNELgt-nANIVSADFIRGTTLVETAIEYN 476 fruit fly
ACE75384     368 kprntlnPLWAIMAELTPRpfdiifn---pngaglRQMADAVNRNLSSLFQNEWwk-qTNIVATDFFLGNNLIQVSIQSN 443 Glyptapanteles ...
EEZ98658     694 ----tlnPMFALMAELTPQpidlff-----rtnnlRKLANDVNRKVTVWFRDEWar-dVNIVATDYFLGNDIINVAIEAN 763 red flour beetle
XP_001658539 258 g---ftnRPVADMAELTPDpwgiit----drygglRKMADSVNRFVTKWYFEELgp-tANIVAVDFIRGTNIIDAAIYWN 329 yellow fever mo...
XP_001122651 298 ----gilRPRSAMAELTPDfkdiis----nrlgdlRDMADRVNLNVTNWYSTIWqy-tANIVATDFVRDTDIVRVAIKSN 368 honey bee
XP_001815277 324 -----gnPLNVLMAELTPNyrnflq----yintnlKDLALMVNRELTEWVIENNfadnLNIIATDFFTGNDMISVAIGTN 394 red flour beetle
XP_002113330 493 ------hGIWLLQGIFKLTeeskv-------srniQDLANIINPNLSRWMDDDWihknINVIVSDFYLGNNIISMAIERN 559 Trichoplax adha...
XP_001843372 327 -----pdAPKSIGWSFHGVqgyqssssseeiylmpKERANLTNPKLMRMLGGPWsl-rANAVLLDFFTNTNLIDMAVHTN 400 southern house ...
XP_002433767 329 g--iatsSMVQMTARFPRNlv------------snRKLAQQVNFPATGWFRDTWwq-cANLVAMDFFLGSNIINVAIDAN 393 black-legged tick
NP_648828    360 -----dsPVRNVGWIFTAVrsmeqtln-ahqlqtaKERAAVLNPKINKWLKGPWgy-nANVVAMDYFSNTNIVDLAIQVN 432 fruit fly
Feature 1           
NP_609541    477 ARK 479 fruit fly
ACE75384     444 IKK 446 Glyptapanteles indiensis
EEZ98658     764 NNR 766 red flour beetle
XP_001658539 330 QKR 332 yellow fever mosquito
XP_001122651 369 KNR 371 honey bee
XP_001815277 395 FRK 397 red flour beetle
XP_002113330 560 LAR 562 Trichoplax adhaerens
XP_001843372 401 RYK 403 southern house mosquito
XP_002433767 394 LRR 396 black-legged tick
NP_648828    433 AHK 435 fruit fly

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