Conserved Protein Domain Family
PI-PLCXDc_like_2

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cd08621: PI-PLCXDc_like_2 
Catalytic domain of uncharacterized hypothetical proteins similar to eukaryotic phosphatidylinositol-specific phospholipase C, X domain containing proteins
This subfamily corresponds to the catalytic domain present in a group of uncharacterized hypothetical proteins found in bacteria and fungi, which are similar to eukaryotic phosphatidylinositol-specific phospholipase C, X domain containing proteins (PI-PLCXD). The typical eukaryotic phosphoinositide-specific phospholipase C (PI-PLC, EC 3.1.4.11) has a multidomain organization that consists of a PLC catalytic core domain, and various regulatory domains. The catalytic core domain is assembled from two highly conserved X- and Y-regions split by a divergent linker sequence. In contrast, eukaryotic PI-PLCXDs contain a single TIM-barrel type catalytic domain, X domain, and are more closely related to bacterial PI-PLCs, which participate in Ca2+-independent PI metabolism, hydrolyzing the membrane lipid phosphatidylinositol (PI) to produce phosphorylated myo-inositol and diacylglycerol (DAG). Although the biological function of eukaryotic PI-PLCXDs still remains unclear, it may distinct from that of typical eukaryotic PI-PLCs.
Statistics
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PSSM-Id: 176558
View PSSM: cd08621
Aligned: 12 rows
Threshold Bit Score: 308.537
Threshold Setting Gi: 116061035
Created: 8-Mar-2010
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
catalytic siteputative active
Feature 1:catalytic site [active site]
Evidence:
  • Comment:Both prokaryotic and eukaryotic PI-PLCs utilize a similar catalytic mechanism, a general base and acid catalysis involving two well conserved histidines. It consists of two steps, a phosphotransfer and a phosphodiesterase reaction.
  • Comment:Based on structure evidence of the catalytic site in Bacillus cereus phosphatidylinositol-specific phospholipase C.
  • Citation:PMID 2509427
  • Citation:PMID 8386017
  • Citation:PMID 7664726

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                          #                                                              
EEU38718      203 YPRISCLKLRELAIPGTHDSGMSQFNgggsg-------------------------fgapLNSKTQELSVAGQLEFGARW 257  Nectria haema...
XP_663986     216 YRVIKDRPLQHLVMPGTHDSGMSTISgkivs-------------------------igseANTQTQGLNIYDQLRVGARW 270  Aspergillus n...
XP_001227387 1049 KEVIKDRPLRHVVMPGSHDSGMSKLTgailt-------------------------gassSNTQTQGLSTYDQLRMGARW 1103 Chaetomium gl...
EEH18278     1186 LNIIGDRKVKHVCMPGSHDAGMSKLDghtq--------------------------fadeGNTLTQYLSVYDQLRRGSRY 1239 Paracoccidioi...
XP_388243     138 RGTLGKRPLSQLCLMGTHDSGMSTTShslvp------------------------vspidPYVLCQSEDIHGQLELGARY 193  Gibberella ze...
XP_001257407 1086 YAIIKDRELRHVVVPGSHDAGMSSITdsgwn------------------------glgtaSNTETQSLDHYNQLRVGVRY 1141 Neosartorya f...
CBI56107      239 YSRMHCLTLREMSLPGTHNSGMSLLTggtalv----------------------karpesVQCHASHMTVGKQLEAGARW 296  Sordaria macr...
XP_365150    1113 LPVMGGRKLRHISMLGSHNAGISVRTggti---------------------------gpsDASICQGVDVLGQLQAGARW 1165 Magnaporthe g...
ZP_05042432    13 YESIKDKALNEICWPATHDSGMSFVNddlws------------------------vgvndCNVVTQNKGIDDQLKAGARV 68   Alcanivorax s...
CAL56423        3 DDAIRDKPIASLVLPGTHDSGTAWLSrellpgsdsqlpkwateavnaaerfglpaddvvrRWAKAQTLTIGEQLRRGARY 82   Ostreococcus ...
ZP_01690463   185 GNLNDSLHLDQITMPGSHDAGMSSLHhcnpa-------------------------lladTYTRTQSLSVGGQMACGARY 239  Microscilla m...
ACT92079       26 GRLADSTKLNGIVMPGSHDAGMSQTDhcgag------------------------gdlnpGLSQTQGLNILDQLKAGSRY 81   Dyadobacter f...
Feature 1                                 #                                                       
EEU38718      258 FDIRPSKtg---------gRWATGHYSFaagnw---------------------------------hggnGEYLDDIIDG 295  Nectria haema...
XP_663986     271 FDLRIMSvhqsd---pssyEFWVAHVNDetadv--------------------------------pvgntGESLADIINE 315  Aspergillus n...
XP_001227387 1104 FDLRVQTvhqvapkccddyEFWTTHISDeraka--------------------------------pigqsGEHLDEVVDN 1151 Chaetomium gl...
EEH18278     1240 FDVRPAIgn--------ggKYLTGHYSYvdvlgi------------------------------gwqggnGESIQEIVDG 1281 Paracoccidioi...
XP_388243     194 FDIRPQIyk---------kKWCTGHYTGkvg-------------------------------------arGESIPSIIEG 227  Gibberella ze...
XP_001257407 1142 FDMRIVSidg-------laSFWAAHVNDetaaa--------------------------------plgatGESLDSLIAG 1182 Neosartorya f...
CBI56107      297 FDIRPVIag---------gKWATGHYTFfdgiklpsgldkipglgdlakeigkigggdkekvldgwqggnGQLIADIVRE 367  Sordaria macr...
XP_365150    1166 FDVRPVIgn--------ggQLLTGHYSGqkapv---------------------------------fgisGQAIDDIVGN 1204 Magnaporthe g...
ZP_05042432    69 FDFRPFMwisk-----rggDLYLGHFNYdpas-----------------------------------gacGEDMASALKA 108  Alcanivorax s...
CAL56423       83 VDLRCGWtp--------dgEWRTHHALI------------------------------------------GRMVDELLEE 112  Ostreococcus ...
ZP_01690463   240 FDIRVDYdh---------hNLVTYHRNGsfg-------------------------------------cnGQSLKDVLDE 273  Microscilla m...
ACT92079       82 FDIRVDYdh---------rELVTYHRTGdsg-------------------------------------cnGQPLAAVLNQ 115  Dyadobacter f...
Feature 1                                                                                         
EEU38718      296 INRFTENN---KELIILNVAQGLNsdnfkgdk----dakisqaEWEDVMRQLERINHrvenr-------ggeedISQLRV 361  Nectria haema...
XP_663986     316 VNRFTSENp--GEIIFLKLRYLIGikrlpgg-----airwntaIANKFFSELKKINNrcpnl-------dtttkFQRQKA 381  Aspergillus n...
XP_001227387 1152 INKFTSENp--GEVIILQFRYLIGirkvpslg----piywgakQKDEFFDKLRRINNrcgniptdssqglpekiLADRGI 1225 Chaetomium gl...
EEH18278     1282 INRFTAEN---PELIIINLDLTLDtdngyk--------pfndeQWSKTFDLFEGVKYlqgn---------legdLTERTM 1341 Paracoccidioi...
XP_388243     228 VNKFTKNN---AELIIINFSHSLQsdvedw-------refnkeEWHSLMEELLKLEHlyiled-----kskannLGSLKL 292  Gibberella ze...
XP_001257407 1183 VNRFTTDYt--GEVIVWWVRYMTNldngdltq---anrywsaaLTNAFYDKLEGINNrcpep-------sdggkFNRQPL 1250 Neosartorya f...
CBI56107      368 VNDFTDKYp--GELVIINLSHGLNtdtfsgnp----karltqqEWENLMTELLAVKNrathit------menidLTYQRL 435  Sordaria macr...
XP_365150    1205 VNAFTRDN---AELVVLSLTHAMQtdekyr--------dlddgEWDRVFDAMEKLDNrcgg---------lkgqIPDMKL 1264 Magnaporthe g...
ZP_05042432   109 VKSFIDREgneKEVVFLHFSHCYKwdtgmfeidkmvldpfsydDIESLRREIEGFIGdvmyvs-----tdeklnVGTLSL 183  Alcanivorax s...
CAL56423      113 VKVFLVENp--SEVVIVEMTHFFGgpraed-------verlatVVEEIFGELLAPYPgst------------kaMYDMKV 171  Ostreococcus ...
ZP_01690463   274 TVSFLNAHr--TETVILKFSHIRNhgddh----------ksaeTKAKIDGFLNNYNQa----------------IYTNHR 325  Microscilla m...
ACT92079      116 SIDFIKAYp--SETFILKFSHIRTnrga------------ereIKDRIDQFLSDMRFkp--------------yFYTQKQ 167  Dyadobacter f...
Feature 1                                                                                         
EEU38718      362 SDFIq-----------DRAAVIIIVDaelyktqdrv--dlsafadkgFFRRNQLPLYDRYADe-nNQNKMIEDQLNKLRE 427  Nectria haema...
XP_663986     382 SYFMdq--------ndGKGCVLFLLDgsnipsgv-----pssspsdgIYPASAMTVNDHWSNl-aTTQPVAEDQIAVWSA 447  Aspergillus n...
XP_001227387 1226 GSFMss--------ngGKGCVFIFLDtahlsnmaa---adrvarsdgIYDHRDLPWSDGWPEk-eDTKAVAEFNVNRWAE 1293 Chaetomium gl...
EEH18278     1342 NDYIg----------aGGAAVIVIASggptr------------pqkgIYSNRQFPRYDSYSNs-dDPAAMADDQLAKLKG 1398 Paracoccidioi...
XP_388243     293 DDFIg----------nGKAAVVCIIEewgsmsl-------gdyahkgFYKSSQFNVRNEYSNk-dETEYMVKDQIEKMKD 354  Gibberella ze...
XP_001257407 1251 HTFLda--------ndGRGCVLIIIDgtlnagip------kgraesrIYNTTSFDRDDYWAQe-qYTQQNADKQIAHMKS 1315 Neosartorya f...
CBI56107      436 SNLLppsdpskpndlsRKSSVLFVVDdittdnqhv---nlskfskegFFFRGQLAIFDSYANt-nNLNNMITDQFSKLST 511  Sordaria macr...
XP_365150    1265 GDLIg----------kGRACVIVLADggkvr-----------pdkgiYRPGDSFDFIERWSNv-dKAADMAADQESWVRA 1322 Magnaporthe g...
ZP_05042432   184 KDVVe----------sGRRVIVTIEDknfssdpkggfftiqtprygyIHDQGKCVTYSDSVNvkaFYQYAGTDDLKDMES 253  Alcanivorax s...
CAL56423      172 KDFVda---------nHRAIVVFEDDrta----------------aeWKFWPARTITNTYADt-dDENGMENFNREVVRD 225  Ostreococcus ...
ZP_01690463   326 INVNlakvs--lgevrGKMILVFDYPeyinpatg------rfryqdsDKVLPNLAVFDKYSNs-aTYNDMKVDQLKKWSQ 396  Microscilla m...
ACT92079      168 YDLNlaett--lgecrGKFILVFDYPeyisarsg-----rfryhdgsSYAGPNITVYDSYSKt-tSLDKMKSDQLAKLDT 239  Dyadobacter f...
Feature 1                                                                                         
EEU38718      428 qrqs-----------adsTMFLLSWTLTQAglnvv------------------engkgANRALIEKLWLtms-----sst 473  Nectria haema...
XP_663986     448 inrvs--------pftndQFLISQWLVSADalqttalt------------iqniaimpTNPALYWAGVNams-----ptk 502  Aspergillus n...
XP_001227387 1294 ar---------------sNVLVSQWLSTPNvltstfsys-----------lqaiavlpTNPTLYWRGVPdms-----pta 1342 Chaetomium gl...
EEH18278     1399 nrnivsd-----tterkdTFHIFSWTLTLSrvfersi--------------adqsielGYDPLFWRGYHaft-----pfs 1454 Paracoccidioi...
XP_388243     355 hmss-----------kdkRLFLLSWTLTQQvpawagsvrsladkvgdsikpikflareCNKELFTQLLPeis-----dna 418  Gibberella ze...
XP_001257407 1316 iardg---------gtgdSYYIMQWQCTPSaldvaippp----------tlqlianqeTNPALYHYGLNais-----pdk 1371 Neosartorya f...
CBI56107      512 hrtk-----------addDMFLLSWTLTQPvetmitgs-------------ivkygedANRALGEKLWAngam---saqr 564  Sordaria macr...
XP_365150    1323 nrnlvad-----dkarkdKFAISQWILTVKsvsvltss------------iedyalnrAYDPVFWRGYSsws-----pws 1380 Magnaporthe g...
ZP_05042432   254 kerrmlekysacyksgdgIFSGFSWTLTRVdwafgkc--------------ieklaeeANNAFTGKIEDylkdgvitkei 319  Alcanivorax s...
CAL56423      226 fnkps---------faadAILKLSWTLTTQpktviealdifk----ryprnllqladrANARLSAFTVDatnn----hcs 288  Ostreococcus ...
ZP_01690463   397 hagp-----------gqgIFFLLSWTLTSNhppstgs--------------ikslakqANAGLPEVLKEqivr---qgwq 448  Microscilla m...
ACT92079      240 yggl-----------gkdYLFLLSWTLTPDagtffggs-------------vkklaeeANPELEKVLAEcmsl---pdkp 292  Dyadobacter f...
Feature 1                                            
EEU38718      474 YPNIINIDAYPsn-----------rDFAALSMAIN 497  Nectria haematococca mpVI 77-13-4
XP_663986     503 WPNVLLVDYIGvvitdqfawnqlsaELYTLAIGLN 537  Aspergillus nidulans FGSC A4
XP_001227387 1343 FPNVIMVDYIGqllmndqrweelgaELYTLAIGLN 1377 Chaetomium globosum CBS 148.51
EEH18278     1455 YPNVVYMDFIGsadqsettlekthgEVTALAMAVN 1489 Paracoccidioides brasiliensis Pb03
XP_388243     419 FPNVVYIDYLNn------------rEYLPLVIAIN 441  Gibberella zeae PH-1
XP_001257407 1372 FPTVILHDAVGlfhvsdlsaesynpMMQTLAVGLN 1406 Neosartorya fischeri NRRL 181
CBI56107      565 FPNVVSVDALDed-----------gEVAGLVAGIS 588  Sordaria macrospora
XP_365150    1381 YPSVVLMDFVGiinrgdkkltdmsgEVRALIMAVN 1415 Magnaporthe grisea 70-15
ZP_05042432   320 KPSMVWFDYYSd-------------SCLEPLIKLN 341  Alcanivorax sp. DG881
CAL56423      289 VANIIVVDDLAn------------sDVLEVVARLN 311  Ostreococcus tauri
ZP_01690463   449 RPNIVYVDFVNr-------------ITNQAIVSLN 470  Microscilla marina ATCC 23134
ACT92079      293 RPNIVYIDYLEa-------------GIARHIIQCN 314  Dyadobacter fermentans DSM 18053

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