Conserved Protein Domain Family
PI-PLCXDc_like_1

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cd08620: PI-PLCXDc_like_1 
Catalytic domain of uncharacterized hypothetical proteins similar to eukaryotic phosphatidylinositol-specific phospholipase C, X domain containing proteins
This subfamily corresponds to the catalytic domain present in a group of uncharacterized hypothetical proteins found in bacteria and fungi, which are similar to eukaryotic phosphatidylinositol-specific phospholipase C, X domain containing proteins (PI-PLCXD). The typical eukaryotic phosphoinositide-specific phospholipase C (PI-PLC, EC 3.1.4.11) has a multidomain organization that consists of a PLC catalytic core domain, and various regulatory domains. The catalytic core domain is assembled from two highly conserved X- and Y-regions split by a divergent linker sequence. In contrast, eukaryotic PI-PLCXDs contain a single TIM-barrel type catalytic domain, X domain, and are more closely related to bacterial PI-PLCs, which participate in Ca2+-independent PI metabolism, hydrolyzing the membrane lipid phosphatidylinositol (PI) to produce phosphorylated myo-inositol and diacylglycerol (DAG). Although the biological function of eukaryotic PI-PLCXDs still remains unclear, it may distinct from that of typical eukaryotic PI-PLCs.
Statistics
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PSSM-Id: 176557
View PSSM: cd08620
Aligned: 8 rows
Threshold Bit Score: 365.18
Threshold Setting Gi: 222149024
Created: 4-Mar-2010
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
catalytic siteputative active
Feature 1:catalytic site [active site]
Evidence:
  • Comment:Both prokaryotic and eukaryotic PI-PLCs utilize a similar catalytic mechanism, a general base and acid catalysis involving two well conserved histidines. It consists of two steps, a phosphotransfer and a phosphodiesterase reaction.
  • Comment:Based on structure evidence of the catalytic site in Bacillus cereus phosphatidylinositol-specific phospholipase C.
  • Citation:PMID 2509427
  • Citation:PMID 8386017
  • Citation:PMID 7664726

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                         #                                                              
YP_002603683 301 ATACPGIRFNQLVLPGAHDAGMYEINvdgflwieeaaavllallipv----------pvcntslagllaangfevalaNF 370 Desulfobacteriu...
ZP_01218493  301 AKQNPDFRFRDLVLSGSHDAGMYEIHindpkiaiqkmldsnpv------------------laslelvgtqageqllaNL 362 Photobacterium ...
XP_001401086 180 GSPAAQQPFSRFVLAAPHDNGMNGMTaceaifqhldsdmlaavrrlvp--------qlehvnhvpdhflmkklphivyGL 251 Aspergillus nig...
XP_681239    165 GSPTAQKPFHRFVLPAAHNFGFNTMDncaviasklgpaalvtevllpll-------gplgfighlgpiaaskalgimeAT 237 Aspergillus nid...
YP_002549981 144 NPAVKNAPFASFVLPGAHDAGMFDLTqvntlcnsatgvaalvaaflg----------wipgatilsglaavqlkgviiGE 213 Agrobacterium v...
YP_002017702 137 MNAPAETTLNEFVLPGSHDSGMYVKLtgpl---------------------------------------------gvnAF 171 Pelodictyon pha...
XP_964361    250 GSWQANRRFRRLVLPSAHDAGMNSMAssskllsklggavvgtlvhdnri-------mteiadtlsgpaialiapniifSL 322 Neurospora cras...
YP_745585    214 TPRLASQPFGVFVLPGAHDAGMNGMPrlgdlfannrrldallnsllnnispvgrqllslggtgflralpqstlsrvirNL 293 Granulibacter b...
Feature 1                                                        #                               
YP_002603683 371 SVTQKDTAYNQMISGTRYFDLRPGYkkng-----------dvnKTYHIHNFIPGa-------PFATFLQDVNKFLRENpN 432 Desulfobacteriu...
ZP_01218493  363 SLTQKDNAYTQLVTGTRYFDFRPAYggnv----------fsmnQTYHLHNFIPGv-------VFNDFLTDIDKYLKENsN 425 Photobacterium ...
XP_001401086 252 SITQKKTVSTMLAMGARYFEFRPAKllpmfqk-----vsalrdTFYFQHACIPGl-------AFDEFLREQVAFLDENqT 319 Aspergillus nig...
XP_681239    238 SRNQKDNVPDQLAMGARFFEVRASRlnpqlr------sltglnDLYFHHGMLPGq-------PILTFFQDAVNFLCAQrT 304 Aspergillus nid...
YP_002549981 214 AVTQKDNIQTMLNLGCRYFDFRPGYapsqi--------rpilgDIYHIHNVIPGq-------QLDSFFKDVLTWLVANpS 278 Agrobacterium v...
YP_002017702 172 YNTQKDDVSTQLQLGARYFDFRPGHmwnltaq----nvlvreeRLCHLHSNNGIiaetqageGFENFLQAIVTFLTQHsG 247 Pelodictyon pha...
XP_964361    323 AMTQKDSLDAMLRIGARYFEFRPAYchaavrsamqrgkdglpdKLYFQHGAIPGm-------GYDVFLADVVAFLLAHqE 395 Neurospora cras...
YP_745585    294 AVTQKDDTATMLNLGIRYFDFRPGYifpaga-------seqalGLFHQHNMIPGg-------SYTTFLADVLNWLKANpS 359 Granulibacter b...
Feature 1                                                                                        
YP_002603683 433 EIAVFRITSSGIdkd-kftPLTQDQVEDVAKANItsdvgydf-psnlstfeAQSLSAIAgsGKRVFFLYni------sDV 504 Desulfobacteriu...
ZP_01218493  426 EFAIVRIAKSGIdtd-nftPLNQEQVEACLQASVspsvgyqv-tnsldsfhELTLSQVAk-GKRLIVLFga------sNV 496 Photobacterium ...
XP_001401086 320 EIVTVHIRWDNVvad-cqrPTEDEISNLLNEACAravrgpit--wgtrecfEQPIEELRrtGTRLIVVIe-------aDK 389 Aspergillus nig...
XP_681239    305 EIIVVWFTWNQWse---anPEPETVLKMLQDCFNdaktkgvtlergtfadlDRPVDTLRqeNKRFILSFk-------nDG 374 Aspergillus nid...
YP_002549981 279 EIVVISLGTAGFndhttmdPSAATLQTRFAAAQQssnaesiq--vgsagdlATSYADLIqaNKRLFFLNqpslnwnpaTK 356 Agrobacterium v...
YP_002017702 248 EIVVVKYTNDGFgnntglmPDAGEVEKQIRTVMAksklvr-----gtvsdlAANYAYLVstGKRLIIYDgdd---ssiKE 319 Pelodictyon pha...
XP_964361    396 EIVVVQLRWDGVpne-carPSDQDKRECLDQALKmagdriva--gnendlrHATIAQLRrdKKRLIMMEs-------vDS 465 Neurospora cras...
YP_745585    360 EIVVLHINSQGLynd-amiPSQGTLDSALSSALSqygagik---sgdktafGSSYASLIsaNKRLIVLYqsgs-yrtvTR 434 Granulibacter b...
Feature 1                                                                                        
YP_002603683 505 NDSYSNDTYSKsl-tDPQPVIQALNETVQKtss--sqSLTMLQLQNTGSAslkhyisdiamnstewindlvgsktgniLQ 581 Desulfobacteriu...
ZP_01218493  497 NDSYNDEDYSAsl-nDPSAVITALNSTVQKna---qyQYTVLQLQNTGSAalkhyksqialhvtawlndlvfsgtgnlLQ 572 Photobacterium ...
XP_001401086 390 YDSWTAEAYATl---SADPIIDRFESMTTEgqa--dsDLTILQCQATSQSikevlvysv----------fsaaaasscLT 454 Aspergillus nig...
XP_681239    375 YNPYDNFDTKRhavaDGSGIITELSEMSAEkqk--gtPMTYLQCQTTMSSntyglaaa-------------itfhnrvLL 439 Aspergillus nid...
YP_002549981 357 YDSYSDAYETT----DPQVIMDALKAMTKSgq---tsDYTVLQLQATATGqnvvknvial--------adsmsnaynpLM 421 Agrobacterium v...
YP_002017702 320 RVSYWKGNYATe---NPNEIIAALDKTLNTqigtdkyAATILQVAGSFQGtslgiqmal----------scgthdggpLL 386 Pelodictyon pha...
XP_964361    466 LSTYTDEGNATm---DGQSIVDAFPKVLTEdnw-rgkAFINIQCQATATNipkavaysv----------ldagttsscIL 531 Neurospora cras...
YP_745585    435 YDSYSDGAYGTt---CPASILGALNNMNAGgqs--saDYTVLQLQGTATNtksgaaaai----------lgdgdasspLL 499 Granulibacter b...
Feature 1                                                             
YP_002603683 582 DTKGIFDHATYNWLTQsdviasiqkqksLVVIQNDFVDv-ALAEHAYALCKKR 633 Desulfobacterium autotrophicum HRM2
ZP_01218493  573 ATKPTFDHATYTWLTQpdtveaiakqngPVVVQNDFVDi-ALYQHALALSQQR 624 Photobacterium profundum 3TCK
XP_001401086 455 STKGMLDMQTLPWIREnaldr--lraerTIVLMNDFIDg-ATVDTSIMLSRQR 504 Aspergillus niger CBS 513.88
XP_681239    440 AQKAKTDPLTLDWLYAhvkar--lgddhLLVIMNDFFDl-ATSDVANQLTKTR 489 Aspergillus nidulans FGSC A4
YP_002549981 422 STKPMMDSNTYPWLLEnvtal---sdanLLVLLNDFVDnaLSGCVASVLTAQR 471 Agrobacterium vitis S4
YP_002017702 387 YTKARFDNAVQGWLRSmnnsl--rfdtpLVVLLDDYVDn-ALTELCIHLTQER 436 Pelodictyon phaeoclathratiforme BU-1
XP_964361    532 ATKATCDSKTLPWCRDnvlrt--cgydtLVVMMNDFIDg-ATADVAFQLSKQR 581 Neurospora crassa OR74A
YP_745585    500 ATKPVFDAATIPWVRGvaggr--flprqPLVLLNDFADn-AMTSTAIAVTRQR 549 Granulibacter bethesdensis CGDNIH1

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