Conserved Protein Domain Family
PI-PLCXD1c

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cd08616: PI-PLCXD1c 
Catalytic domain of phosphatidylinositol-specific phospholipase C, X domain containing 1
This subfamily corresponds to the catalytic domain present in a group of phosphatidylinositol-specific phospholipase C X domain containing 1 (PI-PLCXD1), 2 (PI-PLCXD2) and 3 (PI-PLCXD3), which are bacterial phosphatidylinositol-specific phospholipase C (PI-PLC, EC 4.6.1.13) sequence homologs found in vertebrates. The typical eukaryotic phosphoinositide-specific phospholipase C (PI-PLC, EC 3.1.4.11) has a multidomain organization that consists of a PLC catalytic core domain, and various regulatory domains. The catalytic core domain is assembled from two highly conserved X- and Y-regions split by a divergent linker sequence. In contrast, members in this group contain a single TIM-barrel type catalytic domain, X domain, and are more closely related to bacterial PI-PLCs, which participate in Ca2+-independent PI metabolism, hydrolyzing the membrane lipid phosphatidylinositol (PI) to produce phosphorylated myo-inositol and diacylglycerol (DAG). Although the biological function of eukaryotic PI-PLCXDs still remains unclear, it may distinct from that of typical eukaryotic PI-PLCs.
Statistics
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PSSM-Id: 176555
View PSSM: cd08616
Aligned: 30 rows
Threshold Bit Score: 270.267
Threshold Setting Gi: 74858691
Created: 3-Mar-2010
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
catalytic siteputative active
Feature 1:catalytic site [active site]
Evidence:
  • Comment:Both prokaryotic and eukaryotic PI-PLCs utilize a similar catalytic mechanism, a general base and acid catalysis involving two well conserved histidines. It consists of two steps, a phosphotransfer and a phosphodiesterase reaction.
  • Comment:Based on structure evidence of the catalytic site in Bacillus cereus phosphatidylinositol-specific phospholipase C.
  • Citation:PMID 2509427
  • Citation:PMID 8386017
  • Citation:PMID 7664726

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                       #                                                             
Q58EK3     19 LPDTLHGIPLTNLAIPGSHDSFSFYIDEASPVgpeqp----etvqNFVSVFGTVAKKLMr----KWLATQTMNFTSQLEA 90  zebrafish
EEC20275   25 LPEQLHSVPLNYLTIPGSHNSCSYTISSQSDIapdn-----pygnKLLKLLGPIGKQILy----SWSLTQNITTREQLQV 95  Ixodes scapularis
EFC38383   19 NYEKIKKKPLNRIKIPGTHDSFTNSINFRNNDsalmfandspsemWKVFSVPFIPKTFMlmmlvPWANTQSCTATEQLNH 98  Naegleria gruberi
EEB12540   54 LPENLTQIPFIYLAIPGSHNSFSYSISPNSGVspdsp----lvvqNIGKWCKPLGNKIIf----KWSQGQQQDVTRQLYL 125 Pediculus humanus ...
CAZ29512   11 LPAHISQKALNLISIPGSHNSFTYSITNHSPPspdn------sicRLDICLPRSFLSRIl---yPWSVTQSLSLVDQLEA 81  Schistosoma mansoni
NP_510673   8 LPSSIREKPLCSICIPGSHDSGAYWFNLRMGYahdq------sflRYVRNFRCNFVKRIv---eRWGLTQSLPIDEQLQI 78  nematode
EDR28273    9 CNSTIQLKPITKIGMVGSHDSGTSTILPQYGI-------------TPFTIFPKKHRSFMk----RWSKTQQLGIKDQCIA 71  Entamoeba dispar S...
XP_652415  12 GESNVLNLTIKEICLAGSHNCFSYNTKTQIKAa------------EPYDSFPIIMNGFVk----DWSETQTLTILQQLQE 75  Entamoeba histolyt...
EDP28267    4 LPAAARDKPIMTLAIPGSHLSGTCNLKEDSEItpdq------twcVRVLDSNDMIRKAVy----NWSKVQTISIKQQLEA 73  Brugia malayi
Q54BH5    437 NYSKLKDRKVRNLVLPGSHDSATYFINSLSPK-------------SPDADHYKYPDYLLt----PWSKTQTCSVYKQLCF 499 Dictyostelium disc...
Feature 1                                           #                                         
Q58EK3     91 GIRFFDLRISTkprd---------------pdnELYFAHGLFSaTVREGLEQISTFLASHa-REVVFLDFNHFYGVqn-- 152 zebrafish
EEC20275   96 GIRYFDLRIATkkdp---------------adeNLYLIHGLFGaVVDNFFVEVKEFLEGHr-KEVILLHFQHFYNLtd-- 157 Ixodes scapularis
EFC38383   99 GIRYFDVRICKddre--------------sldnQFKICHNFYAgKIMDILSEVRDFVINVapSEFIILDFNHFYGLsq-- 162 Naegleria gruberi
EEB12540  126 GIRYFDIRLVIyk------------------nnDIVVAHGLYGgRIQGFLEEINQFLTSHp-KEFVVLDFQHFYEFkt-- 184 Pediculus humanus ...
CAZ29512   82 GIRYFDFRICArqkclnk---------ckngesGFYLVHGLYAnLLSAELQSILGFLQANp-REVLIVDCNHCYYFetde 151 Schistosoma mansoni
NP_510673  79 GVRFFDIRLELaldinasarqnlaqdpddssrkSCFIVHGLYSiDCLKLAHQMVDFLVAHk-EEVLILNVSHIYRMte-- 155 nematode
EDR28273   72 GIRYFDFRVVYnhk-----------------lkNFYLLHGLYCqLLETALQDITFFLTEHd-GEVIIIDINHLYQIks-- 131 Entamoeba dispar S...
XP_652415  76 GVRYFDLRIQKfk-------------------dDIYTVHGLYCdLFVVILTQLSEFIWYHk-KEVIILDINHIYNMnk-- 133 Entamoeba histolyt...
EDP28267   74 GIRFLDVQVAYin-------------------eDIYVVHGLRSmEIRELFKRVDDFLSVHp-KEIVLIDINHFYEFre-- 131 Brugia malayi
Q54BH5    500 GVRYFDLRVARlk-------------------dKLYIIHNFYSdSVKQVLKDILQYVSENv-NEVIILHWSHLYLVd--- 556 Dictyostelium disc...
Feature 1                                                                                     
Q58EK3    153 ---lHHEKLVQMLRTVFGDRLCPvvf----aqEVSLKYLWe-kEYQVLVFYHNPmale-----------------vpflW 207 zebrafish
EEC20275  158 ---aDHNRLLNKLTSNFGPLMCQftk---dlnNLTLESLWr-rGYQIIAFYRHDtlaky----------------hpylW 214 Ixodes scapularis
EFC38383  163 ---kDHDYLLSKIITQVGDVMIKpsq---fklNSTLESMWantTRRVFIFYNDNylkatsp------------diaeyfH 224 Naegleria gruberi
EEB12540  185 ---eDHERMITLLVNIFDDKICRrdr---rmrENTLDLFRq-fGNQILIVYRHIitkl-----------------ndifW 240 Pediculus humanus ...
CAZ29512  152 qkdcFESTVLKILDSIMFPYQKN---------IPILEELWs-aSKQVIFISCHRespek---------------ihpkfW 206 Schistosoma mansoni
NP_510673 156 --ydFRRFFLHPFSSIAERCGISlcptdvdlrIVTLDELIe-kEFRIIVVGPSDqdmd------------------gccF 214 nematode
EDR28273  132 --lnNFQSLCLLIEKCCSKHLVLndy---nkfIIPLSQLVh-iKQRLFVFCLNPfrqkl----------------plylF 189 Entamoeba dispar S...
XP_652415 134 ---eDNEKILEIISCLFNGISVEcnp---nnlNIPLEVLVs-nNKRVFIFYDYPkevkr----------------fpyvW 190 Entamoeba histolyt...
EDP28267  132 ---qHHDKLLDMISNLFGDRLISrptttrtamSYTLNKIWn-sTGRVIIFYQPPlpslrasdinghagpsdiiklpnhvW 207 Brugia malayi
Q54BH5    557 ---eDNKLLMKMIIEILGKFMSNsnk----gpDVKVGDLA---GTPIICIYDDLvnplsnggaggn--gkrpdirdprlW 624 Dictyostelium disc...
Feature 1                                                                                     
Q58EK3    208 PGQMMPAPWANTt--DPEKLILFLQASVKDRRRk-----gTFFVSQVVLTPKASTVmk----gVTSGLRETITERALPSM 276 zebrafish
EEC20275  215 PTDFIPNPWPNEd--EVKQLVAFWDHTYQHERDp-----eKFLVLQGVGTPQPATVtm----hICNSLRVYLVPKVNGAL 283 Ixodes scapularis
EFC38383  225 TNDDIEYTWGDTl--NINYLNEYNLRKLKQRSEskddisnTLLSLQLVLTPNIGTIv------QGFFNRPYSILDITQPM 296 Naegleria gruberi
EEB12540  241 PSGTWHTPWHDKi--DVNELLECLNKGLTERKDg------MGHVTQAILTPNVKYIt-----lHTFGSLKKLAYKSNEKI 307 Pediculus humanus ...
CAZ29512  207 PSSSIKSFWPETv--GPRAMISFLNNHIKPHLHrp---dnTFSVHQGVLTPSLSFIl-----lHPFGSVHHLAKIAGKYY 276 Schistosoma mansoni
NP_510673 215 RSAALQNKWPRKn--NTSDLLVYLQAQIHAPVTp------GLRVLQGVITPELSDIfr----nLRSSLKKTFSLPMRGLL 282 nematode
EDR28273  190 PQDQIDSIWPNKn--ETQRMLNKLDYNSKLYQNn-----pKLTIIQAVVTPSIKSIrk----sYYTKKYPNSTIKIAEKT 258 Entamoeba dispar S...
XP_652415 191 GQDFIHSYWPDRp--TFQEVSQRILTDGMNWINnr---rtGITILQIILTPTNKSIkscylsvSNSKSLKGVSYKVKENF 265 Entamoeba histolyt...
EDP28267  208 SRQFIRSPWPKTd--DAKRMVDDVGNILQTRVLe-----dGFQVCQAIVTLTVNSIir----qPTGTFEARFGRRATRAL 276 Brugia malayi
Q54BH5    625 DPSCISSPYETSryhSFESILKFLKSRINVPKRk------VLHVCQAILTIEFSYE-------FFGHDLITWTVEHRNKF 691 Dictyostelium disc...
Feature 1                                            
Q58EK3    277 MQWIRSQRPGEs--GVNIITADFVEl--gEFISAVITLN 311 zebrafish
EEC20275  284 ESWLSGKEGGSh--GCNVIMSDFVDmdnfRIPKIVVAMN 320 Ixodes scapularis
EFC38383  297 ISPIQQWIPSYdqsLLNIITCDYYHl--yNFTDIVIQSN 333 Naegleria gruberi
EEB12540  308 VSWLETKTCGMg--GVNVVICDFIEg--yDFTENVIKIN 342 Pediculus humanus corporis
CAZ29512  277 QQWLNKPNQLAgpdGINITLIDFFAttypTYIRDVVSIN 315 Schistosoma mansoni
NP_510673 283 KEWLQRLDDEEi-gNLNILISDQVDt---EFCRLVYYLN 317 nematode
EDR28273  259 NPLVLKWLQTSs-tFVNILLIDNVKmn-dEIIHFMITRN 295 Entamoeba dispar SAW760
XP_652415 266 FQFLHSKELESf--PINILLLDFIQin-dPFIQLCIERS 301 Entamoeba histolytica HM-1:IMSS
EDP28267  277 VDWLRQNGHSYr-sNINVILADFVDe--dDFCNTVIDLN 312 Brugia malayi
Q54BH5    692 NEFFNDLETFAs--PTNIIMTDFVTf--yPLSSYCIRRN 726 Dictyostelium discoideum

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