2FJU,2ZKM


Conserved Protein Domain Family
PI-PLCc_beta

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cd08591: PI-PLCc_beta 
Click on image for an interactive view with Cn3D
Catalytic domain of metazoan phosphoinositide-specific phospholipase C-beta
This subfamily corresponds to the catalytic domain present in metazoan phosphoinositide-specific phospholipase C (PI-PLC, EC 3.1.4.11)-beta isozymes. PI-PLC is a signaling enzyme that hydrolyzes the membrane phospholipids phosphatidylinositol-4,5-bisphosphate (PIP2) to generate two important second messengers in eukaryotic signal transduction cascades, Inositol 1,4,5-trisphosphate (InsP3) and diacylglycerol (DAG). InsP3 triggers inflow of calcium from intracellular stores, while DAG, together with calcium, activates protein kinase C, which goes on to phosphorylate other molecules, leading to altered cellular activity. Calcium is required for the catalysis. PLC-beta represents a class of mammalian PI-PLC that has an N-terminal pleckstrin homology (PH) domain, an array of EF hands, a PLC catalytic core domain, a C2 domain, and a unique C-terminal coiled-coil (CT) domain necessary for homodimerization. The PLC catalytic core domain is a TIM barrel with two highly conserved regions (X and Y) split by a highly degenerate linker sequence. There are four PLC-beta isozymes (1-4). They are activated by the heterotrimeric G protein alpha q subunits through their C2 domain and long C-terminal extension. The beta-gamma subunits of heterotrimeric G proteins are known to activate the PLC-beta2 and -beta3 isozymes only. Aside from four PLC-beta isozymes identified in mammals, some eukaryotic PLC-beta homologs have been classified into this subfamily, such as NorpA and PLC-21 from Drosophila and PLC-beta from turkey, Xenopus, sponge, and hydra.
Statistics
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PSSM-Id: 176533
View PSSM: cd08591
Aligned: 26 rows
Threshold Bit Score: 323.135
Threshold Setting Gi: 4521177
Created: 2-Oct-2009
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 2 residues -Click on image for an interactive view with Cn3D
Feature 1:catalytic site [active site]
Evidence:
  • Comment:Both prokaryotic and eukaryotic PI-PLCs utilize a similar catalytic mechanism, a general base and acid catalysis involving two well conserved histidines. It consists of two steps, a phosphotransfer and a phosphodiesterase reaction.
  • Structure:2FJU; Homo sapiens phospholipase C beta 2 catalytic site residues
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                         #                                              #                
2FJU_B        311 HHDMTQPLNHYFINSSHNTYLTAGQFSGLSSAEMYRQVLLSGCRCVELDCWKGkppdeEPIITHGFTMTTDIFFKEAIEA 390  human
AAA28820      317 CDDMDQPMSHYFINSSHNTYLTGHQLTGKSSVEIYRQCLLAGCRCVELDFWNGrt--eEPVIVHGYTFVPEIFAKDVLEA 394  fruit fly
BAA76276      325 HQDLSFPIAHYFINSSHNTYLQGHQLTGKSSVEIYRQVLLAGCRCIELDCWDGkteeqEPVITHGMTLCTEVLFKDVIEA 404  green hydra
AAF79180      322 GANMKLSLAAYYINSSHNTYLTGHQLTGKSSVEIYRQVLLTGCRCLELDCWDGkd--gEPIITHGFTMCTEVQFKDVVHA 399  longfin insho...
Q9Z1B3        315 NEDMSQPLSHYFINSSHNTYLTAGQLAGNSSVEMYRQVLLSGCRCVELDCWKGrtaeeEPVITHGFTMTTEISFKEVIEA 394  house mouse
EAW74231      249 STDMTQPLSAYFINSSHNTYLTAGQLAGTSSVEMYRQALLWGCRCVELDVWKGrppeeEPFITHGFTMTTEVPLRDVLEA 328  human
ACI25594      316 SEDMTFPLSHYFINSSHNTYLTAGQLAGNSSVEMYKQVLLSGCRCIELDCWKGrtteeEPVTTHGFTMTTEISFKEVIEA 395  Japanese flou...
XP_425261     515 NEDMSQPLSHYFINSSHNTYLTAGQLAGNSSVEMYRQVLLSGCRCVELDCWKGrtaeeEPVITHGFTMTTEISFKEVIEA 594  chicken
NP_001081169  316 SDDMNQPLSSYFINSSHNTYLTAGQLTGNSSVEMYRQVLLTGCRCIELDCWKGrpqdeEPFITHGFTMTTEIPFKEVIEA 395  African clawe...
NP_001116245  317 IDDMNQPLSHYFINSSHNTYLTVGQLTGLSSVEMYRQVLLTGCRCIELDCWKGrppeeEPIITHGFTMTTEIPFKDVIEA 396  Danio rerio
Feature 1                                                                                         
2FJU_B        391 IAESAFKTSPYPIILSFENHVDsprQQAKMAEYCRTIFGDMLLTEPLEKFPLKPGvpLPSPEDLRGKILIKNKKnqfsgp 470  human
AAA28820      395 IAESAFKTSEYPVILSFENHCNp-rQQAKIANYCREIFGDMLLDKPLDSHPLEPNmdLPPPAMLRRKIIIKNKKkhhhhh 473  fruit fly
BAA76276      405 IKESAFKTSEYPVILSFENHCSa-kQQMKMANYCLQIFGDLLLNKELDEYPLLPGtlLPSPKVLKRKILIKNKKreikdt 483  green hydra
AAF79180      400 IAECAFKVSEYPVILSFENHCSv-pQQKLLAQYCHEAFGELLLDNPIDGHPLKPGvpLPTPYDLRKKILIKNKKihkgag 478  longfin insho...
Q9Z1B3        395 IAECAFKTSPFPILLSFENHVDspkQQAKMAEYCRLIFGDALLMEPLEKYPLESGvpLPSPMDLMYKILVKNKKkshkss 474  house mouse
EAW74231      329 IAETAFKTSPYPVILSFENHVDsakQQAKMAEYCRSIFGDALLIEPLDKYPLAPGvpLPSPQDLMGRILVKNKKrhrpsa 408  human
ACI25594      396 IAECAFKTSPFPIILSFENHVDspkQQAKMAEYCRSIFGDALLTEPLERYPLESGvpLPCPMELMGKVLVKNKKkhhktn 475  Japanese flou...
XP_425261     595 IAECAFKTSPFPILLSFENHVDspkQQAKMAEYCRLIFGDALLMDPLEKYPLEPGvpLPSPMDLMYKILVKNKKkshksa 674  chicken
NP_001081169  396 IAESAFKTSPFPVILSFENHVDsskQQAKMAEYCRNIFGDALLIDPLEKYPLQPGvaLPSPQELMGKILVKNKKrhkvav 475  African clawe...
NP_001116245  397 IAESAFKTSPYPLILSFENHVDsakQQAKMAEYCRSMFGDALLIDPLDKYPLVPGqqLPSPQELLGKILIKNKKkhhhra 476  Danio rerio
Feature 1                                                                                         
2FJU_B        471 tssskdtggeaegssppsapavwageegtelee----------------------------------------------- 503  human
AAA28820      474 hhhhhkkpaqvgtpaannklttansvdakaaqqvalsashedggvtrstangdvatgtgtgsaagtaghapplqqirqss 553  fruit fly
BAA76276      484 knanistqsvdlnssgrfepevl--------------------------------------------------------- 506  green hydra
AAF79180      479 dddelagltdeekkk----------------------------------------------------------------- 493  longfin insho...
Q9Z1B3        475 egsgkkklseqasntysdsssvfepsspga-------------------------------------------------- 504  house mouse
EAW74231      409 ggpdsagrkrpleqsnsalsessaatepsspqlgspssdscpglsngeevglek-----------pslepqkslgdegln 477  human
ACI25594      476 gstkkklseqvsntysdsssvcepsspsagstkdemadssqpse-------------------------------gaels 524  Japanese flou...
XP_425261     675 dgsakkklseqasntcsdtssmfepsspga-------------------------------------------------- 704  chicken
NP_001081169  476 kgpegsvkrrvseqtsntysdsssvcessatglppsesadvsltlsn-------------------------gdekieek 530  African clawe...
NP_001116245  477 snggsirrkdgtdeqssplndcplsdgdvgqlmsnggeklaerm--------------------------------akdh 524  Danio rerio
Feature 1                                                                                         
2FJU_B        504 -------eeveeeeeeesgnldeeeikkmqsdegtaglevtayeeMSSLVNYIQPTKFVSFEFSAQKNrsYVISSFTELK 576  human
AAA28820      554 kdstgssdsdsssedeslpnttpnlpsgnepppekaqketeagaeISALVNYVQPIHFSSFENAEKKNrcYEMSSFDEKQ 633  fruit fly
BAA76276      507 -----------------nieeipedaedlskseeeeapeveaqqeLSELVNYFQPVHFRGFEKAEKRKcyYEMSSFGENT 569  green hydra
AAF79180      494 -------------------------iekekkdagtaakeaeaaeeMSALVNYIQPVHFTTFEQAQKKDrhYEMSSMVETQ 548  longfin insho...
Q9Z1B3        505 ----------geadtesddddddddckkssmdegtagseamateeMSNLVNYIQPVKFESFEISKKRNksFEMSSFVETK 574  house mouse
EAW74231      478 rgpyvlgpadredeeedeeeeeqtdpkkpttdegtassevnateeMSTLVNYIEPVKFKSFEAARKRNkcFEMSSFVETK 557  human
ACI25594      525 lrpqgrksigevgaesededdddddckkgsmdegtagseafateeMSNLVIYTQPVKFNSFEASKKINrsYQMSSFVETK 604  Japanese flou...
XP_425261     705 ---------geaeiesdddddydddckkssmdegtagseamateeMSNLVNYIQPVKFESFEVSKKRNksFEMSSFVETK 775  chicken
NP_001081169  531 ppkytkprksidhdayseeeeeeepsdpkksdegtassevnateeMSTLVNYVEPVKFKSFDAAKKRNkyYEMSSFVETK 610  African clawe...
NP_001116245  525 dnsksidrgdgesdeeeeeepipdpkkhnntdegtaysevnateeMSTLVNYIEPVKFKSFEVAAKRKkfFEMSSFVETK 604  Danio rerio
Feature 1                                                                              
2FJU_B        577 AYDLLSKASVQFVDYNKRQMSRIYPKGTRMDSSNYMPQMFWNAGCQMVALNFQTmDLPMQQNMAVFEFN 645  human
AAA28820      634 ATTLLKERPIEFVNYNKHQLSRVYPAGTRFDSSNFMPQLFWNAGCQLVALNFQTlDLAMQLNLGIFEYN 702  fruit fly
BAA76276      570 ATNLLKEQPVEFVNYNKFQNSRIYPKGSRVSSDNYIPQLFWNAGCQMVALNFQTlDVPMQLNLGKFEIN 638  green hydra
AAF79180      549 ALNKLKDNPEDFVDYNKKQLTRIYPKGTRVDSSNYVPQIYWNAGCQLVALNFQCfDVAMCVNLGVFEYN 617  longfin inshore squid
Q9Z1B3        575 GLEQLTKSPVEFVEYNKMQLSRIYPKGTRVDSSNYMPQLFWNAGCQMMALNFQTvDLAMQINMGMYEYN 643  house mouse
EAW74231      558 AMEQLTKSPMEFVEYNKQQLSRIYPKGTRVDSSNYMPQLFWNVGCQLVALNFQTlDVAMQLNAGVFEYN 626  human
ACI25594      605 ALEQLTKSPVEFVEYNKLQLSRIYPKGTRVDSSNYNPQLFWNAGCQLVALNFQTiDLSMQLNLGMYEYN 673  Japanese flounder
XP_425261     776 GLEQLTKSPVEFVEYNKMQLSRIYPKGTRVDSSNYMPQVFWNAGCQMVALNFQTvDLSMQINMGMYEYN 844  chicken
NP_001081169  611 ALEQLTKSPMEFVEYNKKQLSRIYPKGTRVDSSNYMPQLFWNAGCQMVALNFQTlDLPMQLNVGIFEYN 679  African clawed frog
NP_001116245  605 GMDTLKNSPTEFVEYNKKQMSRIYPKGTRVDSSNYMPQLFWNVGCQMVALNFQTlDLAMQLNMGVFEYN 673  Danio rerio

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