Conserved Protein Domain Family
PI-PLCc_Rv2075c_like

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cd08590: PI-PLCc_Rv2075c_like 
Catalytic domain of uncharacterized Mycobacterium tuberculosis Rv2075c-like proteins
This subfamily corresponds to the catalytic domain present in uncharacterized Mycobacterium tuberculosis Rv2075c and its homologs. Members in this family are more closely related to the Streptomyces antibioticus phosphatidylinositol-specific phospholipase C1(SaPLC1)-like proteins rather than the typical bacterial phosphatidylinositol-specific phospholipase C (PI-PLC, EC 4.6.1.13), which participate in Ca2+-independent PI metabolism, hydrolyzing the membrane lipid phosphatidylinositol (PI) to produce phosphorylated myo-inositol and diacylglycerol (DAG). In contrast, SaPLC1-like proteins have two Ca2+-chelating amino acid substitutions which convert them to metal-dependent bacterial PI-PLC. Rv2075c and its homologs have the same amino acid substitutions as well, which might suggest they have metal-dependent PI-PLC activity.
Statistics
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PSSM-Id: 176532
View PSSM: cd08590
Aligned: 10 rows
Threshold Bit Score: 275.824
Threshold Setting Gi: 86165421
Created: 5-Mar-2010
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
Feature 1:catalytic site [active site]
Evidence:
  • Comment:Both prokaryotic and eukaryotic PI-PLCs utilize a similar catalytic mechanism, a general base and acid catalysis involving two well conserved histidines. It consists of two steps, a phosphotransfer and a phosphodiesterase reaction.
  • Comment:Based on the structure evidence of catalytic site in Bacillus cereus phosphatidylinositol-specific phospholipase C.
  • Citation:PMID 2509427
  • Citation:PMID 8386017
  • Citation:PMID 7664726

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                          #                                                             
NP_711556     47 KVQVNMNLPLNRALFFGTHDSYNssayr--------------------rnpsNQTYTITDQLRLGARYLELEVHWttgr- 105 Leptospira inte...
YP_431520     37 QRTLDLGEPLGRANFPYTHNSYNssayanl----------------gsywdpNHIYSLVDQLDMGIRALELDVHYty--- 97  Hahella chejuen...
EAQ66688      50 QRALDNYAPIIDNNILGTHNTYNsevytscnf-----------svgcryldpQQKYSIKDQLRMGARFIELDVHWttkme 118 Marinomonas sp....
XP_001624147  34 QRELQSNASFEKYQMLAAHNAFNdrsdgygemddcr---wpppyhgvcidfaNQEFSFTDLLDMGVRALEIDPWWcf--- 107 starlet sea ane...
ZP_01306661   56 QRNMDANKPLAENNIIGSHNSYNsrkyrna----------------tryldpQQIVSIYDQLRLGARFIELDAHWtahth 119 Oceanobacter sp...
ZP_05060983   45 ERDLDKAAPLRQATFVGTHNSYNssayadi----------------tryidpNQNQSIRAQLDMGARFLEFDVHMtnkfd 108 gamma proteobac...
XP_800669     63 QRELQIDFTFDQFIMLDSHNSFQarayglrygandtc-vwpppypenctsiaNHEFTIVDQLNLGMRGIEIDNWYcy--- 138 purple urchin
Q10683       105 QDALQDPVPLRETQWLGTHNSFNslsdsft----------------vshadsNQQLSLAQQLDIDVRALELDLHYlprle 168 Mycobacterium t...
EEY67465     412 QRNLTYSETLCYAELPATHNSVItqahgygnrdqlfnarlnasnaasymrtsNQFLSLSDQLDLGVRFLELDVHFfa--- 488 Phytophthora in...
EEY61913     383 QRNLAFSGPFCFAQIPATHNSAItladgfgnrdqlfnknlnpdkwwsylktnNQMLSMTDQLDIGIRFLEIDTHFfl--- 459 Phytophthora in...
Feature 1                           #                                                            
NP_711556    106 ----------sgnkELLLCRGSNlndhng----------------------------------------cyrydlTFEAG 135 Leptospira inte...
YP_431520     98 -------------gDLKLCHGANdhtgc------------------------------------------safdrRFEDG 122 Hahella chejuen...
EAQ66688     119 s-------lfsypkRLLLCHGFCsin-----------------------------------------------dkYATEG 144 Marinomonas sp....
XP_001624147 108 -------------gKIRMSHAHDhaylgc-----------------------------------------spwdrEFHYG 133 starlet sea ane...
ZP_01306661  120 gw------pwqwgtDLLLCHSGIgvdvgdlh------------------------------------vgcsltdrRVEDG 157 Oceanobacter sp...
ZP_05060983  109 thgspwawewtsndQLLLCHGQSnhlgc------------------------------------------ssadrYFRDG 146 gamma proteobac...
XP_800669    139 -------------gAMRVCHLGTheylgv----------------------------------------ceadhmLFADL 165 purple urchin
Q10683       169 ---------ghgapGVTVCHGLGpknanl----------------------------------------gctvepLLATV 199 Mycobacterium t...
EEY67465     489 -------------sSLRSAHCSDsgvafvddaasalvsslesvldasgqdstvqwgselvgclpslsgiradeqrLHNES 555 Phytophthora in...
EEY61913     460 -------------nDLRTGHCGSlgseavagffgtlgktlgnygty-------nwgpellgcfpsisgikaseqpLTKDS 519 Phytophthora in...
Feature 1                                                                                        
NP_711556    136 LNEISQWIQKPenqNEVLILYIKDRFegh------vseFMRTLSSKLGTLLYRHqsrdcln----qspmvmPKLEDMVks 205 Leptospira inte...
YP_431520    123 LKEVATWLRQDgnrGEVLIIYLEEHVdgr------yddAVAALNRQMGDLIYKPgscat--------lpmnISKADILn- 187 Hahella chejuen...
EAQ66688     145 FNEIKSWLASSesqGEVIILYIEDDSegh------hsdLYSQLNDRFGDKIYPSqgcgn--------ipdtLTKAQVLa- 209 Marinomonas sp....
XP_001624147 134 IQEIAEWIKRNp--KEVVRIYLEDSGshtkg---hddlINGPIKDYLGDKVLTPndtlvy------fngrwPTVSEMRk- 201 starlet sea ane...
ZP_01306661  158 IAEVARWINENp--KEVIILYFEDHTdgr------hqeLFNVINKQLGANIYASqgcka--------ipntLTKNQVLa- 220 Oceanobacter sp...
ZP_05060983  147 LNELRDFIAANr--DEVVLLYIEDHMdge------yawASDILDNSIGQYLYRPsqhgsgc----qglpnqLTKQDILn- 213 gamma proteobac...
XP_800669    166 LSDIGDWLDQPenqDEIIRLYFNEKEdqgh-----ddeVNAMIRDAFGTRVLTPsdlrdt------yggswPTIRKMRe- 233 purple urchin
Q10683       200 LPQIANWLNAPghtEEVILLYLEDQLknasayesvvatLDQVLRRADGTSLIYRpnparratngcvplpldVSREEIRa- 278 Mycobacterium t...
EEY67465     556 LGEIATWLSSHp--DDLVVLYTEIGDevgty--sqseaLLELYTTIFGDLLFSPsdfdda-----ggdwngFTLQELIs- 625 Phytophthora in...
EEY61913     520 MDEIKAWLNANp--TEFVVVYLDTGAdikrs--dkfgaIDTLFTDTFGDSLVPLkalddla----kgkwtgGRINDFIn- 590 Phytophthora in...
Feature 1                                                                                        
NP_711556    206 tNHRIFLTSnncyspeLSDTWGYYfrk-------dpfVSFQPSGFRGYPDCNfsre----tyhNSLIRVYNDTia----- 269 Leptospira inte...
YP_431520    188 sGRQVLLIGgnc--gsDAWAQTVYny----------gFPTDNDHFHPYPECRtdkyd-lnfvqNNLVRIFEDStrl---- 250 Hahella chejuen...
EAQ66688     210 qGKQIILWKdg----gCSSNANMAn-----------lAFTGLGNVGRIWEDA-----------TTLGTIAEFFdggi--- 260 Marinomonas sp....
XP_001624147 202 lGKTVVVATg-----nLYNHKGMYihk--------syWQEMTYNKFLSQANCsam-----gnnSIPIRVYSDStk----- 258 starlet sea ane...
ZP_01306661  221 sGKQVIVWKdg----gCSGNQNMSn-----------mAFTSLGDINRIWEDR-----------TSIGAIGAFFtngsv-- 272 Oceanobacter sp...
ZP_05060983  214 sGRNVVVITgg----gCSGNAQYDa-----------rVWGQNFNTRNTANAAncdglsrsghdSALVRYYEDRtnl---- 274 gamma proteobac...
XP_800669    234 dGKHVLIAAg-----gTYGFFTHGdvyih---plyfdADLRTNLFTPYPDCSgr-------ndTNIVRVYSDSln----- 293 purple urchin
Q10683       279 sGARAVLVGsca--pgWSAAVFDWsgv--------elESGSNSGYRPYPACDatygr--gvyaWRLVRYYEDStla---- 342 Mycobacterium t...
EEY67465     626 qGKQVILVTt-----pEANDQMFYmrel------cagWADVPSSSTGASGTFfges----mnaGSLVRVFKSVlhyatlt 690 Phytophthora in...
EEY61913     591 aGHQVLALAnt----kTKAAYSLYdmctaekdltvefIDDLPDAKRLINGLAiy-------snTNWIRSWSEQiry---- 655 Phytophthora in...
Feature 1                                                                 
NP_711556    270 ----rnANDRGGSFTNSniqsm-------laceVNLFGFDQFna--nfAKQAVWSWD 313 Leptospira interrogans serovar Lai str...
YP_431520    251 ---sdvFGDPPQPITPElmaqa-------arcsLGVVGLDQLkafderMTAAVWSWD 297 Hahella chejuensis KCTC 2396
EAQ66688     261 --ksltANDVSTAFATG----------------ANIVNLDDMvmndgrIEAAAWSWD 299 Marinomonas sp. MED121
XP_001624147 259 -----yGPFWNGPWKTGtilnym----dflkcgVTYPAADQVnp--hlLATAVFTWA 304 starlet sea anemone
ZP_01306661  273 --kkieSEDVIQAFKNG----------------GNIVNLDDMthsddrLSAAIWSWD 311 Oceanobacter sp. RED65
ZP_05060983  275 ---saaFGNPGEPITTGnieql-------lacgANVIGFDKLdeddgrLERAIWSWG 321 gamma proteobacterium HTCC5015
XP_800669    294 -----fPLNEKGYYSGEetvgsikdlteyvkcrIQYPTLDMInp--dlIKTGVWTWA 343 purple urchin
Q10683       343 ----taLANPTRPPANPqaltppkv-pamtdcgVNLFGFDQLlpedgrIQASLWSWA 394 Mycobacterium tuberculosis
EEY67465     691 esamsgGGAEVDTASEPghvnastl-pvfvdagVNILAPDGLdg--aiMTAMVWSWA 744 Phytophthora infestans T30-4
EEY61913     656 ---islAASGALTRKFPvfldaesi-pkylrwnVNLIALDNAdi--akMAALVWSWA 706 Phytophthora infestans T30-4

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