Conserved Protein Domain Family
PI-PLCc_SaPLC1_like

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cd08589: PI-PLCc_SaPLC1_like 
Catalytic domain of Streptomyces antibioticus phosphatidylinositol-specific phospholipase C1-like proteins
This subfamily corresponds to the catalytic domain present in Streptomyces antibioticus phosphatidylinositol-specific phospholipase C1 (SaPLC1) and similar proteins. The typical bacterial phosphatidylinositol-specific phospholipase C (PI-PLC, EC 4.6.1.13) catalyzes Ca2+-independent hydrolysis of the membrane lipid phosphatidylinositol (PI) to produce phosphorylated myo-inositol and diacylglycerol (DAG). The catalytic mechanism is based on general base and acid catalysis utilizing two well conserved histidines, and consists of two steps, a phosphotransfer and a phosphodiesterase reaction. In contrast, SaPLC1 is the first known natural Ca2+-dependent bacterial PI-PLC. It is more closely related to the eukaryotic PI-PLCs rather than the typical bacterial PI-PLCs. It participates in PI metabolism to generate myo-inositol-1-phosphate and myo-inositol-1:2-cyclic phosphate simultaneously. SaPLC1 and other members in this subfamily have two Ca2+-chelating amino acid substitutions which convert them from metal-independent enzymes to metal-dependent bacterial PI-PLC. Additionally, SaPLC1 active site utilizes a mechanism of amino acid juxtaposition, swapping amino acid positions, to adapt a calcium binding pocket and maintain more ideal active site geometry to support efficient catalysis.
Statistics
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PSSM-Id: 176531
View PSSM: cd08589
Aligned: 31 rows
Threshold Bit Score: 220.729
Threshold Setting Gi: 70733310
Created: 3-Mar-2010
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
Feature 1:catalytic site [active site]
Evidence:
  • Comment:Both prokaryotic and eukaryotic PI-PLCs utilize a similar catalytic mechanism, a general base and acid catalysis involving two well conserved histidines. It consists of two steps, a phosphotransfer and a phosphodiesterase reaction.
  • Comment:Calcium-dependent phosphatidylinositol-specific phospholipase C from Streptomyces antibioticus (saPLC1) catalyzes hydrolysis of phosphatidylinositol into inositol 1-phosphate by a unique mechanism involving formation of inositol 1,6-cyclic phosphate as an intermediate.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                         #                                                              
NP_421719     27 RQRIDALPMTDLLTVGTHNSYKlaippeemaamvaargpvalgidYSHRPLTEQLDAgARQLEIDVVADpqgglfakplt 106 Caulobacter cre...
BAG41444      25 GAEPAATTYGTSTSVGVHNAYEk----------------------EKYRYFADALDSgAALLELDLWSNal--------- 73  Streptomyces an...
ZP_04333335   24 PASAAEQALSRTTSVGVHNAYEt----------------------ATFPHFADALDSgAGLLELDVWTDew--------- 72  Nocardiopsis da...
ZP_05483545   19 VAHADSPKLSHVTTVGVHNTYDp----------------------AAYPYLAQALDNgSSLLELDVWPDff--------- 67  Streptomyces sp...
ZP_04607501   40 AAVDPAARVSATTAVGTHNAYDk----------------------ETYTYLAQALDArPGMIELDVWPDvv--------- 88  Micromonospora ...
YP_003115811  31 AADTSSAPLSHSSVVGLHNTYDd---------------------gSQFPHLANALDTgTSMIELDTWTDvf--------- 80  Catenulispora a...
YP_003384749  24 AATRADASIAGVTGVGVHNAYVq----------------------STFPWLVDALESgASMLELDIWQNfl--------- 72  Kribbella flavi...
YP_001161166  26 AAPANEMPFASATTVGIHNAYEq----------------------TAFDYLAEALDTgTSLIEIDIWPNif--------- 74  Salinispora tro...
NP_630472    128 APGTGRLPFASATTAGLHNTYSp----------------------TAFRYLAQGLDTgTGMIEIDTWPDnt--------- 176 Streptomyces co...
YP_003102985  17 PLPASAATATGATAVGVHNSYEk----------------------GTAPYLVDVLDKrPGLVEIDVWTNfl--------- 65  Actinosynnema m...
Feature 1                                #                                                       
NP_421719    107 algkgvtltpdvaaalakPGFKTLHIPDvdFRSSCv---------------TFVACLKEVRAWSDTHRdHAPILIMLNAK 171 Caulobacter cre...
BAG41444      74 -----------------gRSWRVSHSNPlgNNSNCegaanaselrtksrdqDFAGCLSDMRAWHDAHPgHRPILLKIEMK 136 Streptomyces an...
ZP_04333335   73 -----------------fGTWRVNHELVg-QHNNCagatspde-lrrggdgDLADCLRDVRTWHEANPgHPPLLLKVEMK 133 Nocardiopsis da...
ZP_05483545   68 -----------------tHEWKVSHSTPlgNGNNCvdahsaadlysggtnkDLESCLDDIRIWLGAHPgRGPLTLKIEMK 130 Streptomyces sp...
ZP_04607501   89 -----------------aNQWRVSHSNPlgNNNNCvaatsasqlytgtrnkNLEHCLDDIRLWLAAHPdAGPLHLKLELK 151 Micromonospora ...
YP_003115811  81 -----------------tTEWKVSHSNPlgNSNNCvnaskpadiytgsankDLRSCLDDVKYWLASHStTGPLYIKLELK 143 Catenulispora a...
YP_003384749  73 ----------------gsRAYWVGHDPG--NANNCssattfaalrsgsrnqSLPACLRNLRLWHDQNPnHAPVILKLELK 134 Kribbella flavi...
YP_001161166  75 -----------------tGKWKVSHDEPlsNNNNCttgsaltdlytggrnkHFSDCLDNIRVWLDAHPdSGPLMIKVEPK 137 Salinispora tro...
NP_630472    177 -----------------tKQWKVSHSHPhrNVNNCtpattaaqlysgtthsNLETCLDDVRIWLRAHPgAGPLVVKLELK 239 Streptomyces co...
YP_003102985  66 ----------------ftNDFKVSHDPG--SNNNCanastyeqlrtgardqNLAMCLRNVRLWHDRNPnHPPVVLKLEFK 127 Actinosynnema m...
Feature 1                                                                                        
NP_421719    172 EGaaslpggvtplpftealfdaLDAEIRAVFGedrLITPDQVQGKa-----kTLRDGVLAGGWPKLGAARGKVFFALDe- 245 Caulobacter cre...
BAG41444     137 DGfnakgg---------rgpaeFDALIRQKLGd-aVYGPGDLTGGh-----aTADEAVRAGGWPSRADLAGKFLFELIpg 201 Streptomyces an...
ZP_04333335  134 KGyhaeag---------lgpaeFDALADHVLGd-aLFRPDDLLAGah----aTLDEAVRADGWPSRGALAGRVVVYLTpg 199 Nocardiopsis da...
ZP_05483545  131 TGfsantg---------lgpaqLDAAFRDHLGd-aVFKPADLLGSh-----aTLDEASKADNWPTLDALRGKVLTEIIpg 195 Streptomyces sp...
ZP_04607501  152 TGfsartg---------qgpaqLDALLAARLGs-aVFRPADLKGGy-----aTLDAAARADNWPTRQQLTGQVIVHLIpg 216 Micromonospora ...
YP_003115811 144 QGfeansg---------lgpaqLDALINAHLGs-lVYRPSDLLNGty----pTLDAAAKADAWPARSALAGKVILYVIpg 209 Catenulispora a...
YP_003384749 135 NGfdgrng---------fgpaqFDALLTNALGagaILRPAEVKGSa-----aTLDAAVRANGWPSRESLRGRFVVLVEtg 200 Kribbella flavi...
YP_001161166 138 LGlatvfg---------mgvnqVDNLVRSRLGd-rVFRPADLLAKpdggwydSLDEAARAGNWPTREELAGRVLLHVVpg 207 Salinispora tro...
NP_630472    240 AGfatgqg---------mgpsqLDTLIAAHLGd-mVFRPADLLPKptggsyaTASDAARADNWPTRSELAGRVLLYAIp- 308 Streptomyces co...
YP_003102985 128 NGfdgrgg---------fgapqFDQVVANNLGrdvVFGPSDLKGSh-----aTLDAAARAGAWPSRSALAGKFVLLAEtg 193 Actinosynnema m...
Feature 1                                                                                        
NP_421719    246 ---SPAKVALYrgq---raSLEGRAMFVNTDEASpaaaYLTLNDPVAQKdriaa--avkagFIVRTRADAETLQARRNDp 317 Caulobacter cre...
BAG41444     202 tveEKNPFDKLwtdveyagHLKDLAAQGKLAQSTa---FPAVHGAAPGDpreryadpalrpWFVVFDGDAPTYLNGSIDt 278 Streptomyces an...
ZP_04333335  200 tfeQGNPFDDLwtdeeqasRLRDLAAGGRLDEAAt---FPAVLGARGGDprerya-eelrpWFVVFDGSAPTYTGGIDTs 275 Nocardiopsis da...
ZP_05483545  196 tveEGNPTDTLhtdveyagYLLAQKAAGKLNSVQi---FPTVHGAAGGDprdkys-aeqkpWFVVFDGDASEWLAKAGMs 271 Streptomyces sp...
ZP_04607501  217 tveEGNPTDKLwtdieyaqHLAALAAAGTLGSAQa---FPAVHRAQAGDprtryadaalrpWFVVFDGDASTYVTGGIDt 293 Micromonospora ...
YP_003115811 210 tveLGNPFDTLhtdveyatYLKNLAAAGKASQAAv---FPSVLGAQTGDprsqysdtsirpWFVVFDGDANTYVTSVDTs 286 Catenulispora a...
YP_003384749 201 afeAGNPLDHYdtdleyadHLISLNAAGNLAGATm---FTTVNGASATDprtgerggarapWYVTFDGNASTWYAGSTAf 277 Kribbella flavi...
YP_001161166 208 tveNRNPFDWAptneqyaeHLRDLAAAGEIEKAQi---FPTAKGTAGDDprdqyk-asirpWFVVFDSGASGWINRDTTw 283 Salinispora tro...
NP_630472    309 --gSHTPHPDIel----grHLRDLAATGNIGRAQv---FPAVHGATAGDprtaykdtairpWFVVFDGDATTYLDGLNTt 379 Streptomyces co...
YP_003102985 194 tfeAGNPFDSYhtdlevadHLISANAAGALGSTTv---FPAINGASQTDprtgarggarapWFVAFDGSASAYASYPGDs 270 Actinosynnema m...
Feature 1                                                  
NP_421719    318 tqrdaalaSGAQYVSTDYIwadprfagdytvrlPGNKVAVCN 359 Caulobacter crescentus CB15
BAG41444     279 sw---ydtRHYLLIMTDAHnvppvidgthpteaEALARVRQL 317 Streptomyces antibioticus
ZP_04333335  276 wy----geNDYLLVMTDAHavspai--dartpsPEEAAARVR 311 Nocardiopsis dassonvillei subsp. dassonvillei DSM 43111
ZP_05483545  272 wy----ddNHYYVVMTDAQnvapai--ddhnptVDQATQRVA 307 Streptomyces sp. AA4
ZP_04607501  294 aw---ydtNHYLLVMTGAQnvppav--gdtdpdAAAARVAEL 330 Micromonospora sp. ATCC 39149
YP_003115811 287 wy----dtNHYLLTMTDAQnvapal--sdtapsQSDAQARVA 322 Catenulispora acidiphila DSM 44928
YP_003384749 278 y-----taNNYLLVMVDAHnvapai--darnptVQQATDRVR 312 Kribbella flavida DSM 17836
YP_001161166 284 f-----aeNNYLLVMTSAHsvdpa----isrtnPTEEEARDR 316 Salinispora tropica CNB-440
NP_630472    380 wy----dtNHYLLTMTDAHkvvpa----lsatrPSLAQARAR 413 Streptomyces coelicolor A3(2)
YP_003102985 271 y-----lgGHYLVVMTDAHsvapai--dsrtpsVADAQARVR 305 Actinosynnema mirum DSM 43827

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