Conserved Protein Domain Family
PI-PLCc_At5g67130_like

?
cd08588: PI-PLCc_At5g67130_like 
Catalytic domain of Arabidopsis thaliana PI-PLC X domain-containing protein At5g67130 and its uncharacterized homologs
This subfamily corresponds to the catalytic domain present in Arabidopsis thaliana PI-PLC X domain-containing protein At5g67130 and its uncharacterized homologs. Members in this family show high sequence similarity to bacterial phosphatidylinositol-specific phospholipase C (PI-PLC, EC 4.6.1.13), which participates in Ca2+-independent PI metabolism, hydrolyzing the membrane lipid phosphatidylinositol (PI) to produce phosphorylated myo-inositol and diacylglycerol (DAG).
Statistics
?
PSSM-Id: 176530
View PSSM: cd08588
Aligned: 22 rows
Threshold Bit Score: 264.577
Threshold Setting Gi: 66048002
Created: 3-Mar-2010
Updated: 2-Oct-2020
Structure
?
Aligned Rows:
 
catalytic siteputative active
Feature 1:catalytic site [active site]
Evidence:
  • Comment:Both prokaryotic and eukaryotic PI-PLCs utilize a similar catalytic mechanism, a general base and acid catalysis involving two well conserved histidines. It consists of two steps, a phosphotransfer and a phosphodiesterase reaction.
  • Comment:Based on structure evidence of the catalytic site in Bacillus cereus phosphatidylinositol-specific phospholipase C.
  • Citation:PMID 2509427
  • Citation:PMID 8386017
  • Citation:PMID 7664726

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
Feature 1                            #                                                           
Q93XX5        71 TSPTSIINGLPFNKYTWLMTHNAFSNanapllpgveritfyNQEDTITNQLQNGVRGLMLDMYDf--------------- 135 thale cress
CAJ89944     434 CQGSAELCDARYDEIAQLASHNAMATtadr-------figpLQDPDIVGQLDAGVRVLLLDTHHwerpkevadrlsssdf 506 Streptomyces am...
EEU46088      20 CNNSPDLCSRSYNNVTHMGAHGSSFLrdssts---tfgaagNQNYNATDALGAGIRLLQAQVHKe--------------- 81  Nectria haemato...
EER28108     124 CNGYPEFCERKYSNITHIAAHNSPFVrrgn--------iagNQELDVTIQLNDGIRMLQFQTHYi--------------- 180 Coccidioides po...
XP_001885837  32 CNGHAGLCDRKYGNTTFLGSHDSFAVstsvf------alarTQEVDVPSQLRRGVRMLQAQGHMn--------------- 90  Laccaria bicolo...
XP_570978     24 CNGHSELCSRLYSNVTFIGAHDSYAVgss---------vadDQDKDVTSQLNDGIRTLQIQAHNa--------------- 79  Cryptococcus ne...
YP_710462    439 CNGGRGLCGLRYDEAVYAASHNAMASsaad-------fvgaTQDPDLVGQLDTGVRALLLDVQHwttptqvetflaglrp 511 Frankia alni AC...
YP_003397460 404 CNGPRAYCDLRLDEYTFPGTHNSFSAahepg------wlipNQRFGIARQLDAGIRAFLLDVHVgvktdqlvrtdlqaeg 477 Conexibacter wo...
XP_002289114 158 CNGSPANCPLPLDEMMFGMVHNAMSSeegdf------iigyNHYYGLEKALVAGYRGINLDVCSc--------------- 216 Thalassiosira p...
XP_002178081 102 CNGLSTLCDATVDSVMYAALHNAMATsedgf------flgpNHDYKLEDALKWGYRGINLDIGNc--------------- 160 Phaeodactylum t...
Feature 1                                               #                                        
Q93XX5       136 --------------------------------NNDIWLCHSLRgqcfnftafqPAINILREVEAFLSQNPTEIVTIIIED 183 thale cress
CAJ89944     507 p--------------pelrrrltrilervnppHPGLWLCHSVCgag-----alDLVPTLRQIGDWLRAHPTEVVTLVLQD 567 Streptomyces am...
EEU46088      82 --------------------------------NSTLRLCHTTCeil----dagPLEDWLSNVNDWIVANPNEVVTFLLVN 125 Nectria haemato...
EER28108     181 --------------------------------NGTIRLCHSSCdll----dvgPLEDYLRKVADWLRANPYDVVSILMGN 224 Coccidioides po...
XP_001885837  91 --------------------------------GGKLHFCHTTCgly----dggTVEQYLKDVKYFLDRHPNEVLTFIFTN 134 Laccaria bicolo...
XP_570978     80 --------------------------------SDGIHLCHSSCsll----dggLMSDYLSTVASWVNDNPNDVITIVIVN 123 Cryptococcus ne...
YP_710462    512 ----------------rerdalaplargarsaRPGLWLCHSVCqfg-----svNLEDALRSVDDWLARNPSEVVTLILQD 570 Frankia alni AC...
YP_003397460 478 sdrnkvgkvigpanlataerlagrvgagdlrgRREPFLCHTLCelg-----avPAKEQLRAFGRFLDRNRGEVLLFMMEP 552 Conexibacter wo...
XP_002289114 217 --------------------------------GGVLQFCHNVCdlg-----erMPNEVFTNTLQFLNDYPSEVLVLLFEA 259 Thalassiosira p...
XP_002178081 161 --------------------------------NGELQFIHGRCllg-----srNVVEVLTNINTFLTENPSEVVILPLQI 203 Phaeodactylum t...
Feature 1                                                                                        
Q93XX5       184 YVhrpk-------glstLFANAGLDKYWFPVSKmprkgedWPTVTDMVqENHRLLVFTSVaake------deeGVAYQWR 250 thale cress
CAJ89944     568 GVgpvp--------sqgAFERAGLSDLLYEPDAdpd--rpWPKLEDMIdGGRRLVVFAEKadg-------papWYRNFYR 630 Streptomyces am...
EEU46088     126 SDkasps------eigkAFNDSGIADLAYRPSGegp-sgdWPTLEDMIsGNQRVVAFVTNidps-----tdypFLMPEFD 193 Nectria haemato...
EER28108     225 SNfilpt------nytkPIENSGLIDYVYTPSKipmalddWPLLSHFIlTGQRAIVYLDYkanq-----tevpYLLDEFS 293 Coccidioides po...
XP_001885837 135 PEnlsvd-----kvwkpVFDSSGITNLTYVPPQpvmarddWPTLGEMIaSGKRVVVFLDKgaenkt--dpssaFILPQFQ 207 Laccaria bicolo...
XP_570978    124 SDnlppt------sfspVFESAGLSSKVYTPASqptqlsdWPSLSDMIdAGTTVVAFMDYeadt-----ssvpYLLDEFA 192 Cryptococcus ne...
YP_710462    571 SVppge--------viaAFRRVGLLHRIVTPPAdph--grWPTLGHLVaTDRRLVVFAENadv-------pktWYRRFFR 633 Frankia alni AC...
YP_003397460 553 YLppaq--------marLFREAGLGDDVVTLDRaa----pLPTLGDLVrADRRLLVFTEGegg-------vppWYMPAWS 613 Conexibacter wo...
XP_002289114 260 SSeqgpisw--sdlyseMANVDGFTDLLYVLTEgq----aWPTMGELVsSNKRIIVFFFNggscdn--dvcpsEFHPWFT 331 Thalassiosira p...
XP_002178081 204 DNsvgagtidlfdiysiMQSVPGFTDRMYVHPEvt---teWPTLGELVeTDKRILFFHYGgpscwdnsspcppGFHDWFY 280 Phaeodactylum t...
Feature 1                                                                                        
Q93XX5       251 YMVENESGdp-----gvKRGSCPNRKESQPLNsks----ssLFLMNYFPTYPvekda----------------ckehsAP 305 thale cress
CAJ89944     631 YGMETPFAfr-----spDEMSCLPNRGGTDKR---------LFLLNHFVTAGggrrlda-------------glvnsrQR 683 Streptomyces am...
EEU46088     194 YVFETAFEvq-----nlGDFNCTLDRPSKLDSataalssnyLSLVNHFKYQSlvegsdlfvpdvnnieivnsdnttqdGN 268 Nectria haemato...
EER28108     294 QMWETPFSpt------nRDFPCVVHRPPGLSAeda---kkrLYMANHNLNTEvslagasllvpnt-vllnetnavsgyGS 363 Coccidioides po...
XP_001885837 208 MVWEDPYDpt------dPKWPCKVDRTAGPLAps-----qqLNLMNHNLNVNlipvghgvrlp----drlssprtngiRS 272 Laccaria bicolo...
XP_570978    193 AMWEDAYGvt------tQEFGCAVNRSSGDTSs-------qPFLINHFLDSTysfssiqvfvpnk-dklnetnaetgtGS 258 Cryptococcus ne...
YP_710462    634 YGADTPFDvp-----spAGFTCRVGRGSRTAP---------MLLVNHWVEGDdpgrtya-------------dsvnrePA 686 Frankia alni AC...
YP_003397460 614 FFQDTPLGat-----kpSEFSCRRTRGDADSP---------LLLINHWIDAFppnprr--------------nreigdGF 665 Conexibacter wo...
XP_002289114 332 YAAETQFEsaslndlqdYEYSCQVTRGPGSTSdekt-qqasFLVVNNFVTPPdpdaaa---------------vanskDF 395 Thalassiosira p...
XP_002178081 281 YGAETRFSfsdvdairdTTSSCEITRGENSRRr--------FFSVNNFVTLPssnaag---------------vlnrlNF 337 Phaeodactylum t...
Feature 1                                                  
Q93XX5       306 LAEMVGTCLKSggNRMPNFLAVNFYMrs-dgGGVFEILDRMN 346 thale cress
CAJ89944     684 VLERAHNCERRr-GRPVNFVAVDYATi----GDALGAVEELN 720 Streptomyces ambofaciens ATCC 23877
EEU46088     269 LGKHLQECRQEw-SAPPNFVLVDFFEd----GQVLAAADTMN 305 Nectria haematococca mpVI 77-13-4
EER28108     364 AGAMAGNCTEQw-TRPPNFILVDYYNignfnGSVFEVAANCN 404 Coccidioides posadasii C735 delta SOWgp
XP_001885837 273 ILKHAYNCAPIveNRAPNFVMLDYVNi----GQGLEAVNRLN 310 Laccaria bicolor S238N-H82
XP_570978    259 IGYHVNNCRQLw-GRNPNHILLDFYDsn--gNSPFNVAASLN 297 Cryptococcus neoformans var. neoformans JEC21
YP_710462    687 LLAHLRRCERA--GLTPTFVATDFTTi----GDLVPTVAALN 722 Frankia alni ACN14a
YP_003397460 666 LTRRIARCEREr-GMRANVVAVDFYDr----SDVVEASRGLN 702 Conexibacter woesei DSM 14684
XP_002289114 396 LANRLAECANLs-GKRPNFVYLDFWSe----GVTAELVQYAN 432 Thalassiosira pseudonana CCMP1335
XP_002178081 338 VQQHVQQCSALndGLDVNLVFVDFWHk----GNLPEAVQLHN 375 Phaeodactylum tricornutum CCAP 1055/1

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap