Conserved Protein Domain Family
PI-PLCXDc_like

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cd08587: PI-PLCXDc_like 
Catalytic domain of phosphatidylinositol-specific phospholipase C X domain containing and similar proteins
This family corresponds to the catalytic domain present in phosphatidylinositol-specific phospholipase C X domain containing proteins (PI-PLCXD) which are bacterial phosphatidylinositol-specific phospholipase C (PI-PLC, EC 4.6.1.13) sequence homologs mainly found in eukaryota. The typical eukaryotic phosphoinositide-specific phospholipase C (PI-PLC, EC 3.1.4.11) have a multidomain organization that consists of a PLC catalytic core domain, and various regulatory domains. The catalytic core domain is assembled from two highly conserved X- and Y-regions split by a divergent linker sequence. In contrast, eukaryotic PI-PLCXDs and their bacterial homologs contain a single TIM-barrel type catalytic domain, X domain, which is more closely related to that of bacterial PI-PLCs. Although the biological function of eukaryotic PI-PLCXDs still remains unclear, it may be distinct from that of typical eukaryotic PI-PLCs.
Statistics
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PSSM-Id: 176529
View PSSM: cd08587
Aligned: 66 rows
Threshold Bit Score: 165.593
Threshold Setting Gi: 219125146
Created: 3-Mar-2010
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
catalytic siteputative active
Feature 1:catalytic site [active site]
Evidence:
  • Comment:Both prokaryotic and eukaryotic PI-PLCs utilize a similar catalytic mechanism, a general base and acid catalysis involving two well conserved histidines. It consists of two steps, a phosphotransfer and a phosphodiesterase reaction.
  • Comment:Based on structure evidence of the catalytic site in Bacillus cereus phosphatidylinositol-specific phospholipase C.
  • Citation:PMID 2509427
  • Citation:PMID 8386017
  • Citation:PMID 7664726

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                          #                                                              
Q58EK3         20 PDTLHGIPLTNLAIPGSHDSFSFYIDeaspvgpeqpetvqnfvsvfgtvakklmrKWLATQTMNFTSQLEAGIRFFDLRI 99   zebrafish
XP_663986     216 YRVIKDRPLQHLVMPGTHDSGMSTISgkivs--------------------igseANTQTQGLNIYDQLRVGARWFDLRI 275  Aspergillus n...
XP_001227387 1049 KEVIKDRPLRHVVMPGSHDSGMSKLTgailt--------------------gassSNTQTQGLSTYDQLRMGARWFDLRV 1108 Chaetomium gl...
EEH18278     1186 LNIIGDRKVKHVCMPGSHDAGMSKLDghtq---------------------fadeGNTLTQYLSVYDQLRRGSRYFDVRP 1244 Paracoccidioi...
XP_388243     138 RGTLGKRPLSQLCLMGTHDSGMSTTShslvp-------------------vspidPYVLCQSEDIHGQLELGARYFDIRP 198  Gibberella ze...
XP_001257407 1086 YAIIKDRELRHVVVPGSHDAGMSSITdsgwn-------------------glgtaSNTETQSLDHYNQLRVGVRYFDMRI 1146 Neosartorya f...
XP_365150    1113 LPVMGGRKLRHISMLGSHNAGISVRTggti----------------------gpsDASICQGVDVLGQLQAGARWFDVRP 1170 Magnaporthe g...
ZP_05042432    13 YESIKDKALNEICWPATHDSGMSFVNddlws-------------------vgvndCNVVTQNKGIDDQLKAGARVFDFRP 73   Alcanivorax s...
ZP_01690463   185 GNLNDSLHLDQITMPGSHDAGMSSLHhcnpa--------------------lladTYTRTQSLSVGGQMACGARYFDIRV 244  Microscilla m...
ACT92079       26 GRLADSTKLNGIVMPGSHDAGMSQTDhcgag-------------------gdlnpGLSQTQGLNILDQLKAGSRYFDIRV 86   Dyadobacter f...
Feature 1                            #                                                            
Q58EK3        100 STkprd-----pdnELYFAHGLFs------------aTVREGLEQISTFLASHa--REVVFLDFNHFYGvqn-------- 152  zebrafish
XP_663986     276 MSvhqsd---pssyEFWVAHVNDetadvp--vgntgeSLADIINEVNRFTSENp--GEIIFLKLRYLIGikrlpgga--- 345  Aspergillus n...
XP_001227387 1109 QTvhqvapkccddyEFWTTHISDerakap--igqsgeHLDEVVDNINKFTSENp--GEVIILQFRYLIGirkvpslg--- 1181 Chaetomium gl...
EEH18278     1245 AIgn--------ggKYLTGHYSYvdvlgigwqggngeSIQEIVDGINRFTAEN---PELIIINLDLTLDtdngyk----- 1308 Paracoccidioi...
XP_388243     199 QIyk---------kKWCTGHYTGkvga-------rgeSIPSIIEGVNKFTKNN---AELIIINFSHSLQsdvedwr---- 255  Gibberella ze...
XP_001257407 1147 VSidg-------laSFWAAHVNDetaaap--lgatgeSLDSLIAGVNRFTTDYt--GEVIVWWVRYMTNldngdltqa-- 1213 Neosartorya f...
XP_365150    1171 VIgn--------ggQLLTGHYSGqkapvf---gisgqAIDDIVGNVNAFTRDN---AELVVLSLTHAMQtdekyr----- 1231 Magnaporthe g...
ZP_05042432    74 FMwisk-----rggDLYLGHFNYdpasg-----acgeDMASALKAVKSFIDREgneKEVVFLHFSHCYKwdtgmfeidkm 143  Alcanivorax s...
ZP_01690463   245 DYdh---------hNLVTYHRNGsfgc-------ngqSLKDVLDETVSFLNAHr--TETVILKFSHIRNhgddh------ 300  Microscilla m...
ACT92079       87 DYdh---------rELVTYHRTGdsgc-------ngqPLAAVLNQSIDFIKAYp--SETFILKFSHIRTnrga------- 141  Dyadobacter f...
Feature 1                                                                                         
Q58EK3        153 -------lhHEKLVQMLRTVFGdrlcp---------vvfaqEVSLKYLWe--------kEYQVLVFYhnpmale------ 202  zebrafish
XP_663986     346 --irwntaiANKFFSELKKINNrcpnld-------tttkfqRQKASYFMdq------ndGKGCVLFLldgsnipsgvps- 409  Aspergillus n...
XP_001227387 1182 -piywgakqKDEFFDKLRRINNrcgniptdssqglpekilaDRGIGSFMss------ngGKGCVFIFldtahlsnmaaad 1254 Chaetomium gl...
EEH18278     1309 ---pfndeqWSKTFDLFEGVKYlqgnl---------egdltERTMNDYIg--------aGGAAVIVIasggptrp----- 1363 Paracoccidioi...
XP_388243     256 ---efnkeeWHSLMEELLKLEHlyiledk-----skannlgSLKLDDFIg--------nGKAAVVCIieewgsmslgd-- 317  Gibberella ze...
XP_001257407 1214 -nrywsaalTNAFYDKLEGINNrcpeps-------dggkfnRQPLHTFLda------ndGRGCVLIIidgtlnagipk-- 1277 Neosartorya f...
XP_365150    1232 ---dlddgeWDRVFDAMEKLDNrcggl---------kgqipDMKLGDLIg--------kGRACVIVLadggkvrpd---- 1287 Magnaporthe g...
ZP_05042432   144 vldpfsyddIESLRREIEGFIGdvmyvst-----deklnvgTLSLKDVVe--------sGRRVIVTIedknfssdpkggf 210  Alcanivorax s...
ZP_01690463   301 ----ksaetKAKIDGFLNNYNQai----------------yTNHRINVNlakvslgevrGKMILVFDypeyinpatgr-- 358  Microscilla m...
ACT92079      142 -----ereiKDRIDQFLSDMRFkpy--------------fyTQKQYDLNlaettlgecrGKFILVFDypeyisarsgrf- 201  Dyadobacter f...
Feature 1                                                                                         
Q58EK3        203 ---------vpflwpGQMMPAPWANt-tDPEKLILFLQASVKDrrr-------------kgTFFVSQVVLTPKastvmkg 259  zebrafish
XP_663986     410 ----sspsdgiypasAMTVNDHWSNl-aTTQPVAEDQIAVWSAinrvs--------pftndQFLISQWLVSADalqttal 476  Aspergillus n...
XP_001227387 1255 ---rvarsdgiydhrDLPWSDGWPEk-eDTKAVAEFNVNRWAEar---------------sNVLVSQWLSTPNvltstfs 1315 Chaetomium gl...
EEH18278     1364 -------qkgiysnrQFPRYDSYSNs-dDPAAMADDQLAKLKGnrnivsd-----tterkdTFHIFSWTLTLSrvfersi 1430 Paracoccidioi...
XP_388243     318 -----yahkgfykssQFNVRNEYSNk-dETEYMVKDQIEKMKDhmss-----------kdkRLFLLSWTLTQQvpawags 380  Gibberella ze...
XP_001257407 1278 ----graesriynttSFDRDDYWAQe-qYTQQNADKQIAHMKSiardg---------gtgdSYYIMQWQCTPSaldvaip 1343 Neosartorya f...
XP_365150    1288 -------kgiyrpgdSFDFIERWSNv-dKAADMAADQESWVRAnrnlvad-----dkarkdKFAISQWILTVKsvsvlts 1354 Magnaporthe g...
ZP_05042432   211 ftiqtprygyihdqgKCVTYSDSVNvkaFYQYAGTDDLKDMESkerrmlekysacyksgdgIFSGFSWTLTRVdwafgkc 290  Alcanivorax s...
ZP_01690463   359 ----fryqdsdkvlpNLAVFDKYSNs-aTYNDMKVDQLKKWSQhagp-----------gqgIFFLLSWTLTSNhppstgs 422  Microscilla m...
ACT92079      202 ----ryhdgssyagpNITVYDSYSKt-tSLDKMKSDQLAKLDTyggl-----------gkdYLFLLSWTLTPDagtffgg 265  Dyadobacter f...
Feature 1                                                                                       
Q58EK3        260 vts---------glretiterALPSMMQWIRSqrpg-----esgVNIITADFVEl------------gEFISAVITLN 311  zebrafish
XP_663986     477 t------------iqniaimpTNPALYWAGVNamsp-----tkwPNVLLVDYIGvvitdqfawnqlsaELYTLAIGLN 537  Aspergillus nid...
XP_001227387 1316 ys-----------lqaiavlpTNPTLYWRGVPdmsp-----tafPNVIMVDYIGqllmndqrweelgaELYTLAIGLN 1377 Chaetomium glob...
EEH18278     1431 --------------adqsielGYDPLFWRGYHaftp-----fsyPNVVYMDFIGsadqsettlekthgEVTALAMAVN 1489 Paracoccidioide...
XP_388243     381 vrsladkvgdsikpikflareCNKELFTQLLPeisd-----nafPNVVYIDYLNn------------rEYLPLVIAIN 441  Gibberella zeae...
XP_001257407 1344 pp----------tlqlianqeTNPALYHYGLNaisp-----dkfPTVILHDAVGlfhvsdlsaesynpMMQTLAVGLN 1406 Neosartorya fis...
XP_365150    1355 s------------iedyalnrAYDPVFWRGYSswsp-----wsyPSVVLMDFVGiinrgdkkltdmsgEVRALIMAVN 1415 Magnaporthe gri...
ZP_05042432   291 --------------ieklaeeANNAFTGKIEDylkdgvitkeikPSMVWFDYYSd-------------SCLEPLIKLN 341  Alcanivorax sp....
ZP_01690463   423 --------------ikslakqANAGLPEVLKEqivrq---gwqrPNIVYVDFVNr-------------ITNQAIVSLN 470  Microscilla mar...
ACT92079      266 s-------------vkklaeeANPELEKVLAEcmslp---dkprPNIVYIDYLEa-------------GIARHIIQCN 314  Dyadobacter fer...

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