1GYM,1PTG,1AOD,1T6M,3EA3


Conserved Protein Domain Family
PI-PLCc_BcPLC_like

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cd08586: PI-PLCc_BcPLC_like 
Click on image for an interactive view with Cn3D
Catalytic domain of Bacillus cereus phosphatidylinositol-specific phospholipases C and similar proteins
This subfamily corresponds to the catalytic domain present in Bacillus cereus phosphatidylinositol-specific phospholipase C (PI-PLC, EC 4.6.1.13) and its sequence homologs found in bacteria and eukaryota. Bacterial PI-PLCs participate in Ca2+-independent PI metabolism, hydrolyzing the membrane lipid phosphatidylinositol (PI) to produce phosphorylated myo-inositol and diacylglycerol (DAG). Although their precise physiological function remains unclear, bacterial PI-PLCs may function as virulence factors in some pathogenic bacteria. Bacterial PI-PLCs contain a single TIM-barrel type catalytic domain. Their catalytic mechanism is based on general base and acid catalysis utilizing two well conserved histidines, and consists of two steps, a phosphotransfer and a phosphodiesterase reaction. This family also includes some uncharacterized eukaryotic homologs, which contains a single TIM-barrel type catalytic domain, X domain. They are similar to bacterial PI-PLCs, and distinct from typical eukaryotic PI-PLCs, which have a multidomain organization that consists of a PLC catalytic core domain, and various regulatory domains, and strictly require Ca2+ for their catalytic activities. The prototype of this family is Bacillus cereus PI-PLC, which has a moderate thermal stability and is active as a monomer.
Statistics
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PSSM-Id: 176528
View PSSM: cd08586
Aligned: 61 rows
Threshold Bit Score: 213.3
Threshold Setting Gi: 145603422
Created: 3-Mar-2010
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 2 residues -Click on image for an interactive view with Cn3D
Feature 1:catalytic site [active site]
Evidence:
  • Comment:Both prokaryotic and eukaryotic PI-PLCs utilize a similar catalytic mechanism, a general base and acid catalysis involving two well conserved histidines. It consists of two steps, a phosphotransfer and a phosphodiesterase reaction.
  • Structure:1GYM; Bacillus cereus phosphatidylinositol-specific phospholipase C catalytic site
    View structure with Cn3D
  • Citation:PMID 7664726
  • Citation:PMID 2509427
  • Citation:PMID 8386017

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                           #                                                         #   
1GYM_A         14 MQPIPDSIPLARISIPGTHDSGTFKlqnpi----kqvwGMTQEYDFRYQMDHGARIFDIRGRLtd----dnTIVLHHGPL 85   Bacillus cereus
ZP_04780121     9 MSQLDDNKRLSEMTIPGTHDSGTYTlgdgp----vdsaAKCQTQSIAEQLNNGIRYLDIRLARgte--ddgVLWLYHGKE 82   Sphingobacter...
XP_001404552   43 CHTRNSIIPVNQLSIPGTHDSCARSqv---------iyVQTQTQTVSEQLFSGVRALDLRLRRrs----ngQLFCYHGGV 109  Magnaporthe g...
ZP_01772446     1 MSHVDGSKYLDELSIPGTHDSGTCSvdndte--pqtslAKCQQDYIPTQLLEGIRYFDIRLGKndd---kgDPGIDHGIC 75   Collinsella a...
ZP_06289821    25 LAAINDDVFLYELSIPGTHDACTGNgfvekdertgkqyALTQDVCLGNQWKSGIRAFDLRPAVhidsvgnpSLAIFHGTW 104  Prevotella ti...
ZP_04578874    45 MLAINNFTLLSGISMPGTHDSAAFRkwy-------kspYTCHDTSITEQLQGGIRVLDIRLKTkr-----sQVVTCHGDV 112  Oxalobacter f...
EEZ27078       51 MKVLQDTLPVCKISIPGTHDSGSTKgg---------cmLKTQTADIPAQLQKGIRAFDIRLKEkn-----gKLGVFHSHA 116  Bacteroides s...
YP_001783775   12 ITQCNPDLRITELIIPGTHDSATSTcye--------ryYRTQDLSIAEQLDCGVRFLDIRLRK--------EMVAAHREW 75   Haemophilus s...
ZP_03168375   362 MANIPDDALLSSVNIPATHDTGTAGvveddi--pqvsiTSCQNLYYDEQLNMGARSFDIRANAtkddasvaDVKIVHGGE 439  Ruminococcus ...
ZP_04848008   360 ITPLDDNIYVSQLSIPGTHDAATGDgtt-------fslGKTQDMTLDQQFEMGIRAFDLRPALna----ssTMILCHGIV 428  Bacteroides s...
Feature 1                                                                                         
1GYM_A         86 YLy--------VTLHEFINEAKQFLKDNP-SETIIMSLKKEYedmk---------gaedSFSSTFEKKYFVDpifl---- 143  Bacillus cereus
ZP_04780121    83 RAiav---slnLTFEQVINDCLRFLDKNP-QETIIMSVKREFgnd-----------ihdWVKNTIEKRSDWKkrwwl--- 144  Sphingobacter...
XP_001404552  110 PLgsf---grpLALSTVMTEVWAFMIQTGfTETVLVSIDNDDpadres------aegrhAFYEAVDDFIRQTprwgkgeg 180  Magnaporthe g...
ZP_01772446    76 YLlkkd--ggyMQLSNVIGYFKTFLNEHP-SEALIMLVSRGNdea-----------tdeSVTTAFANVMGNNsdlfy--- 138  Collinsella a...
ZP_06289821   105 QTr--------LSMKEALVTLCDSLRKNP-SEFAIVLMRHENsadk----------ndeRWAGLMEELLHAPafenlf-- 163  Prevotella ti...
ZP_04578874   113 GPne------fQPFNDVLDECHRFLTTNP-SEAIVMILKVDDwadyr--------ndpsGGKKLIKNGLENFrsily--- 174  Oxalobacter f...
EEZ27078      117 FQd-------iYWEEDVLLAFISFLQAHP-SETLIVSLKKEGge-------------ikDYASLLSASLNTPayqryf-- 173  Bacteroides s...
YP_001783775   76 VSd--------IRAEEIFEKCGEFLQKNP-QEFVLMRIQNANerkd----------dfpEYGEALLSKVQQYkslfyawe 136  Haemophilus s...
ZP_03168375   440 LWqcqekngsdLTLQSILNTSLGFLEKHK-SETVILTVKSDAgsti---------glehAVAEFIEKNKDKVysg----- 504  Ruminococcus ...
ZP_04848008   429 ATt--------FVWDNVMERFKYYLKENP-GEFIIAIMRHEDeysgtsithwtnentheKWAPAMLEKLNVMkntinpst 499  Bacteroides s...
Feature 1                                                                                         
1GYM_A        144 ---------------ktegNIKLGDARGKIVLLKRYSgsnepggynnfywp--------------------------dne 182  Bacillus cereus
ZP_04780121   145 ---------------fndkNPLLKDVRGKIVLFRRFDgadfgldtqggwp----------------------------dd 181  Sphingobacter...
XP_001404552  181 gragpdggegssrwylapqTPMLGQVRGKAILLRRFAaegghctspraahgnsssedsktt-----dgmgfdlsawvnns 255  Magnaporthe g...
ZP_01772446   139 ---------------tsshVPTLNEVRGKIVLLRRFRlagdsvdghtwgldltewddkikahsgksmclvkyeqgfeaag 203  Collinsella a...
ZP_06289821   164 --------------vayhpHLTLGEMRGKILLLSRDVyadkpigayvrgwthep-------------------dfalqsk 210  Prevotella ti...
ZP_04578874   175 ---------------rgksMPTMNEVRGKIVLLNRIDdsldfgapvsiphn-------------------------vagt 214  Oxalobacter f...
EEZ27078      174 -------------iadfhpELTLKSCRGKILFLHRDHamdnypgaacigwddn----------------------ttcll 218  Bacteroides s...
YP_001783775  137 hs--------qivqdgnpkWPTIAQAAGKIVPIECAPpsmsinkinge-------------------------------- 176  Haemophilus s...
ZP_03168375   505 -----------------gnIPSMKEARGKIIFLRRFNltknyessveramgfnlanw-------------ddikykdyky 554  Ruminococcus ...
ZP_04848008   500 n---------qsytidfrpDLTVGEMRGKILFMCRSWtkyndagpvvggyhdwsh------------------skdggev 552  Bacteroides s...
Feature 1                                                                                         
1GYM_A        183 tftttvnqnaNVTVQDKYKv---------sYDEKVKSIKDTMDETMNNsed---------------lnHLYINFTSLSSg 238  Bacillus cereus
ZP_04780121   182 segplshhniNFYIQDVYNswsd---dddrKDKFNVYVKNCLIRASTDsk-----------------eKFYINFASATGf 241  Sphingobacter...
XP_001404552  256 pdftiqtphaQFRIQDRWKyseri-rlgdlVESKSDLVKNLMDQAIQQdrergll------dvtqhhdLWFIHFTSAVGe 328  Magnaporthe g...
ZP_01772446   204 ntgdkepystAVYAQDHYDc---------tGSSKIDWVDMALKAASEFerstvditaadgttvqaterCWFINYTSCTQn 274  Collinsella a...
ZP_06289821   211 gelyhedsqgALYVQDFYDtseg----kqaVALKCQSIKRLLDFSTRSrqqe------------nahcPLVINHTSGYAe 274  Prevotella ti...
ZP_04578874   215 yladsvnrqyRVYVQDKCElysqdytrsaaKTYKFNCFMDALRKRGNEgq----------------wgEVYLNFASACFf 278  Oxalobacter f...
EEZ27078      219 tlrnkdgkeaTVFLQDEYQyns-----gkeAGKKIAACIRNFDRICEEqtf---------------srRWGISFVSATGl 278  Bacteroides s...
YP_001783775  177 lwaanwhdnsQILLQDLWDgp--------tVEEKCVAIKTLIEKGREVhs-----------------dILLLNHISATNg 231  Haemophilus s...
ZP_03168375   555 ayklyddgknHVYIQDAYNt---------yGSEKWPYILETMKQTTGQdtshp-----------ieynSWVFNYTSCSRg 614  Ruminococcus ...
ZP_04848008   553 siwgpssvigTLNIQDCYNrseagvssdefSTVKWNAIEALLEKSRYFhtnp------------aminRWSYNHTSGYTs 620  Bacteroides s...
Feature 1                                                                                         
1GYM_A        239 gta--------------wnsPYYYASYINPEIANYIKQknp----------arVGWVIQDYINekw-------splLYQE 287  Bacillus cereus
ZP_04780121   242 lggll----------pltsnPRDMAEAVLPLFYQYLSEsap----------arYGIIPMDFPEig---------snLTKM 292  Sphingobacter...
XP_001404552  329 pfkhgeiatarsiavgdfvgWGKWQPGVNIAIAQFIKQyapsg------rrqrLGVVFMDFAVlpe-------dagLLDS 395  Magnaporthe g...
ZP_01772446   275 -------------------nPFTAARVVNEHLYKSSYInntgvegtkencrkhIGIIASDFVDa-----------aLARS 324  Collinsella a...
ZP_06289821   275 svmvqge------riatsegYRKNASITNQYVIHYLKNlsh---------tgaLGIIMMDYAGvdisgrhevkgftLTKE 339  Prevotella ti...
ZP_04578874   279 krftvgrk----qvdipmfgIYIMDSFLNEIGKGSNLPr-------------aLGWLMFDYPFekyytt-ecgyvnVVDI 340  Oxalobacter f...
EEZ27078      279 hs----------------giPLVFANKVNKPVADYLKEnrk----------rnCGIVFIDFIEssg-------gqkLVEY 325  Bacteroides s...
YP_001783775  232 el----------------qyPDAYAAYLNEYSSRLWKQlak---------rsfRGVQIYDFINp-----------sICQE 275  Haemophilus s...
ZP_03168375   615 -------------------aPLGLTREINPRLFKDEGNcid---------nrfLGTVMLNFIDd-----------pMARL 655  Ruminococcus ...
ZP_04848008   621 giia-----------tasttDGYRENAANNHIKFYNKItstd-------wvgsTGIILSDFVGarrsgnftvygdlLPQA 682  Bacteroides s...
Feature 1              
1GYM_A        288 VIRAN 292  Bacillus cereus
ZP_04780121   293 LINCN 297  Sphingobacterium spiritivorum ATCC 33861
XP_001404552  396 IIATN 400  Magnaporthe grisea 70-15
ZP_01772446   325 IYQRN 329  Collinsella aerofaciens ATCC 25986
ZP_06289821   340 IIAHN 344  Prevotella timonensis CRIS 5C-B1
ZP_04578874   341 VIGAN 345  Oxalobacter formigenes OXCC13
EEZ27078      326 LIGGN 330  Bacteroides sp. 2_1_16
YP_001783775  276 LLRLN 280  Haemophilus somnus 2336
ZP_03168375   656 IYETN 660  Ruminococcus lactaris ATCC 29176
ZP_04848008   683 IINNN 687  Bacteroides sp. 1_1_6

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