1GYM,1T6M,1PTG,3EA3,1AOD


Conserved Protein Domain Family
PI-PLCc_bacteria_like

?
cd08557: PI-PLCc_bacteria_like 
Click on image for an interactive view with Cn3D
Catalytic domain of bacterial phosphatidylinositol-specific phospholipase C and similar proteins
This subfamily corresponds to the catalytic domain present in bacterial phosphatidylinositol-specific phospholipase C (PI-PLC, EC 4.6.1.13) and their sequence homologs found in eukaryota. Bacterial PI-PLCs participate in Ca2+-independent PI metabolism, hydrolyzing the membrane lipid phosphatidylinositol (PI) to produce phosphorylated myo-inositol and diacylglycerol (DAG). Although their precise physiological function remains unclear, bacterial PI-PLCs may function as virulence factors in some pathogenic bacteria. Bacterial PI-PLCs contain a single TIM-barrel type catalytic domain. Its catalytic mechanism is based on general base and acid catalysis utilizing two well conserved histidines, and consists of two steps, a phosphotransfer and a phosphodiesterase reaction. Eukaryotic homologs in this family are named as phosphatidylinositol-specific phospholipase C X domain containing proteins (PI-PLCXD). They are distinct from the typical eukaryotic phosphoinositide-specific phospholipases C (PI-PLC, EC 3.1.4.11), which have a multidomain organization that consists of a PLC catalytic core domain, and various regulatory domains. The catalytic core domain is assembled from two highly conserved X- and Y-regions split by a divergent linker sequence. In contrast, eukaryotic PI-PLCXDs contain a single TIM-barrel type catalytic domain, X domain, which is closely related to that of bacterial PI-PLCs. Although the biological function of eukaryotic PI-PLCXDs still remains unclear, it may be distinct from that of typical eukaryotic PI-PLCs. This family also includes a distinctly different type of eukaryotic PLC, glycosylphosphatidylinositol-specific phospholipase C (GPI-PLC), an integral membrane protein characterized in the protozoan parasite Trypanosoma brucei. T. brucei GPI-PLC hydrolyzes the GPI-anchor on the variant specific glycoprotein (VSG), releasing dimyristyl glycerol (DMG), which may facilitate the evasion of the protozoan to the host's immune system. It does not require Ca2+ for its activity and is more closely related to bacterial PI-PLCs, but not mammalian PI-PLCs.
Statistics
?
PSSM-Id: 176500
View PSSM: cd08557
Aligned: 190 rows
Threshold Bit Score: 79.0588
Threshold Setting Gi: 70733310
Created: 28-Sep-2009
Updated: 2-Oct-2020
Structure
?
Program:
Drawing:
Aligned Rows:
 
catalytic siteactive site
Conserved site includes 2 residues -Click on image for an interactive view with Cn3D
Feature 1:catalytic site [active site]
Evidence:
  • Comment:Both prokaryotic and eukaryotic phosphatidylinositol-specific phospholipase Cs utilize a similar catalytic mechanism, a general base and acid catalysis involving two well conserved histidines. It consists of two steps, a phosphotransfer and a phosphodiesterase reaction.
  • Structure:1GYM; Bacillus cereus phosphatidylinositol-specific phospholipase C catalytic site
    View structure with Cn3D
  • Citation:PMID 2509427
  • Citation:PMID 8386017
  • Citation:PMID 7664726

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
Feature 1                            #                                                            
1GYM_A         15 QPIPDSIPLA--RISIPGTHDSGTfklqnpi---------------------------kqvwgmTQEYDFRYQMDHgARI 65   Bacillus cereus
CAJ89944      437 SAELCDARYD--EIAQLASHNAMAttadr-------------------------------figpLQDPDIVGQLDAgVRV 483  Streptomyces ...
YP_710462     442 GRGLCGLRYD--EAVYAASHNAMAssaad-------------------------------fvgaTQDPDLVGQLDTgVRA 488  Frankia alni ...
YP_003397460  407 PRAYCDLRLD--EYTFPGTHNSFSaahep------------------------------gwlipNQRFGIARQLDAgIRA 454  Conexibacter ...
EEU46088       23 SPDLCSRSYN--NVTHMGAHGSSFlrdssts---------------------------tfgaagNQNYNATDALGAgIRL 73   Nectria haema...
EER28108      127 YPEFCERKYS--NITHIAAHNSPFvrrg--------------------------------niagNQELDVTIQLNDgIRM 172  Coccidioides ...
XP_570978      27 HSELCSRLYS--NVTFIGAHDSYAvgss---------------------------------vadDQDKDVTSQLNDgIRT 71   Cryptococcus ...
EER05118      920 LVVNCQKTVV--QITLPSTHNSFSslqdr-------------------------------fvlpNHYFNIKQQLEHgIRG 966  Perkinsus mar...
XP_002289114  161 SPANCPLPLD--EMMFGMVHNAMSseegd------------------------------fiigyNHYYGLEKALVAgYRG 208  Thalassiosira...
YP_237843    1793 HSLNNITFYRskFSGNAATHYWYYptaetenaywikvskdayaapgindftrpfdqytwvtahnAYLDAITPQLERgIRG 1872 Pseudomonas s...
Feature 1                                                                       #                 
1GYM_A         66 FDIRGRLtd----------------------------------------------dnTIVLHHGPLyl-----yvTLHEF 94   Bacillus cereus
CAJ89944      484 LLLDTHHwerpkevadrlsssdfpp--------------elrrrltrilervnpphpGLWLCHSVCgag----alDLVPT 545  Streptomyces ...
YP_710462     489 LLLDVQHwttptqvetflaglrpr----------------erdalaplargarsarpGLWLCHSVCqfg----svNLEDA 548  Frankia alni ...
YP_003397460  455 FLLDVHVgvktdqlvrtdlqaegsdrnkvgkvigpanlataerlagrvgagdlrgrrEPFLCHTLCelg----avPAKEQ 530  Conexibacter ...
EEU46088       74 LQAQVHKen-----------------------------------------------sTLRLCHTTCeil---dagPLEDW 103  Nectria haema...
EER28108      173 LQFQTHYin-----------------------------------------------gTIRLCHSSCdll---dvgPLEDY 202  Coccidioides ...
XP_570978      72 LQIQAHNas-----------------------------------------------dGIHLCHSSCsll---dggLMSDY 101  Cryptococcus ...
EER05118      967 LLLDIHPhnst-------------------------------------------meePLRLCHAMCalg----tlVLRDE 999  Perkinsus mar...
XP_002289114  209 INLDVCScg-----------------------------------------------gVLQFCHNVCdlg----erMPNEV 237  Thalassiosira...
YP_237843    1873 FMLDIHMdvgdy-----------------------------------------ngqkRVRVCHLPAigacwadapLLSDV 1911 Pseudomonas s...
Feature 1                                                                                         
1GYM_A         95 INEAKQFLKDNpSETIIMSLKKEYedmkg------aedsFSSTFEKKYFvdpifl----------ktegnIKLGDAR--G 156  Bacillus cereus
CAJ89944      546 LRQIGDWLRAHpTEVVTLVLQDGVgpvps------qgafERAGLSDLLYepdad-----------pdrpwPKLEDMIdgG 608  Streptomyces ...
YP_710462     549 LRSVDDWLARNpSEVVTLILQDSVppgev------iaafRRVGLLHRIVtppad-----------phgrwPTLGHLVatD 611  Frankia alni ...
YP_003397460  531 LRAFGRFLDRNrGEVLLFMMEPYLppaqm------arlfREAGLGDDVVtldr-------------aaplPTLGDLVraD 591  Conexibacter ...
EEU46088      104 LSNVNDWIVANpNEVVTFLLVNSDkaspse----igkafNDSGIADLAYrpsgeg----------psgdwPTLEDMIsgN 169  Nectria haema...
EER28108      203 LRKVADWLRANpYDVVSILMGNSNfilptn----ytkpiENSGLIDYVYtpskip---------malddwPLLSHFIltG 269  Coccidioides ...
XP_570978     102 LSTVASWVNDNpNDVITIVIVNSDnlppts----fspvfESAGLSSKVYtpasqp---------tqlsdwPSLSDMIdaG 168  Cryptococcus ...
EER05118     1000 MEMVAEWLAANpREVIMLVNEFYGetft-------aleySMEPLIPYMYvhpdaqnetmrilgeavrspwPTLQEMIdtN 1072 Perkinsus mar...
XP_002289114  238 FTNTLQFLNDYpSEVLVLLFEASSeqgpiswsdlysemaNVDGFTDLLYvlte-------------gqawPTMGELVssN 304  Thalassiosira...
YP_237843    1912 LREFVAYMQKDrNAVISLLFESTLssdel-----rpvleQVPELADYSHvsng--------------yswPTLREMIdsN 1972 Pseudomonas s...
Feature 1                                                                                         
1GYM_A        157 KIVLLKRysgsnepggynnfywpdnetftttvnqnaNVTVQDKYkvs----ydeKVKSIKDTMDETMNNsed-------- 224  Bacillus cereus
CAJ89944      609 RRLVVFAekadgpa---------------pwyrnfyRYGMETPFafr-----spDEMSCLPNRGGTDKR----------- 657  Streptomyces ...
YP_710462     612 RRLVVFAenadvpk---------------twyrrffRYGADTPFdvp-----spAGFTCRVGRGSRTAP----------- 660  Frankia alni ...
YP_003397460  592 RRLLVFTegeggvp---------------pwympawSFFQDTPLgat-----kpSEFSCRRTRGDADSP----------- 640  Conexibacter ...
EEU46088      170 QRVVAFVtnidpstd-------------ypflmpefDYVFETAFevq-----nlGDFNCTLDRPSKLDSataal------ 225  Nectria haema...
EER28108      270 QRAIVYLdykanqte-------------vpylldefSQMWETPFsp------tnRDFPCVVHRPPGLSAeda-------- 322  Coccidioides ...
XP_570978     169 TTVVAFMdyeadtss-------------vpylldefAAMWEDAYgv------ttQEFGCAVNRSSGDTSs---------- 219  Cryptococcus ...
EER05118     1073 KRVVVINqhdcysnndnsf------dgcpawdhnfyQFTAENNYawq-----trEMLNCTISARSSKFEfisestlnssd 1141 Perkinsus mar...
XP_002289114  305 KRIIVFFfnggscdndv----------cpsefhpwfTYAAETQFesaslndlqdYEYSCQVTRGPGSTSdekt------- 367  Thalassiosira...
YP_237843    1973 KRLVMLSngevaktytla--------gkqaevlwapDTEVENTYnlgi--tslvHDWQCKSRYGYMDLSlrtrd------ 2036 Pseudomonas s...
Feature 1                                                                                         
1GYM_A        225 -----lnHLYINFTSLSsggtawns--------------pyyyasyINPEIANYIKQknparVGWVIQDYInekw-splL 284  Bacillus cereus
CAJ89944      658 -------LFLLNHFVTAgggrrlda-------------glvnsrqrVLERAHNCERRrg-rpVNFVAVDYAti----gdA 712  Streptomyces ...
YP_710462     661 -------MLLVNHWVEGddpgrtya-------------dsvnrepaLLAHLRRCERAgl--tPTFVATDFTti----gdL 714  Frankia alni ...
YP_003397460  641 -------LLLINHWIDAfppnprrn--------------reigdgfLTRRIARCERErg-mrANVVAVDFYdr----sdV 694  Conexibacter ...
EEU46088      226 ---ssnyLSLVNHFKYQslvegsdlfvpdvnnieivnsdnttqdgnLGKHLQECRQEws-apPNFVLVDFFed----gqV 297  Nectria haema...
EER28108      323 ----kkrLYMANHNLNTevslagasllvpnt-vllnetnavsgygsAGAMAGNCTEQwt-rpPNFILVDYYnignfngsV 396  Coccidioides ...
XP_570978     220 ------qPFLINHFLDStysfssiqvfvpnk-dklnetnaetgtgsIGYHVNNCRQLwg-rnPNHILLDFYdsn--gnsP 289  Cryptococcus ...
EER05118     1142 rtsvqnrLIFVNHFLSIpvpfplts-------------lqvnkddlMREVLDNCTAQwsghvPSMMAVDYWsi----gnI 1204 Perkinsus mar...
XP_002289114  368 ---qqasFLVVNNFVTPpdpdaaa---------------vanskdfLANRLAECANLsg-krPNFVYLDFWse----gvT 424  Thalassiosira...
YP_237843    2037 --gglprLFVLNQFHSWgsttlhagd------------vdnnltwlQRRVENHCGEAtgwrkPNYLGIDFNqv----gdA 2098 Pseudomonas s...
Feature 1                 
1GYM_A        285 YQEVIRAN 292  Bacillus cereus
CAJ89944      713 LGAVEELN 720  Streptomyces ambofaciens ATCC 23877
YP_710462     715 VPTVAALN 722  Frankia alni ACN14a
YP_003397460  695 VEASRGLN 702  Conexibacter woesei DSM 14684
EEU46088      298 LAAADTMN 305  Nectria haematococca mpVI 77-13-4
EER28108      397 FEVAANCN 404  Coccidioides posadasii C735 delta SOWgp
XP_570978     290 FNVAASLN 297  Cryptococcus neoformans var. neoformans JEC21
EER05118     1205 THVAHEYN 1212 Perkinsus marinus ATCC 50983
XP_002289114  425 AELVQYAN 432  Thalassiosira pseudonana CCMP1335
YP_237843    2099 LPYAATLS 2106 Pseudomonas syringae pv. syringae B728a

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap