3FDW


Conserved Protein Domain Family
C2A_SLP

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cd08521: C2A_SLP 
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C2 domain first repeat present in Synaptotagmin-like proteins
All Slp members basically share an N-terminal Slp homology domain (SHD) and C-terminal tandem C2 domains (named the C2A domain and the C2B domain) with the SHD and C2 domains being separated by a linker sequence of various length. Slp1/JFC1 and Slp2/exophilin 4 promote granule docking to the plasma membrane. Additionally, their C2A domains are both Ca2+ independent, unlike the case in Slp3 and Slp4/granuphilin in which their C2A domains are Ca2+ dependent. It is thought that SHD (except for the Slp4-SHD) functions as a specific Rab27A/B-binding domain. In addition to Slps, rabphilin, Noc2, and Munc13-4 also function as Rab27-binding proteins. It has been demonstrated that Slp3 and Slp4/granuphilin promote dense-core vesicle exocytosis. Slp5 mRNA has been shown to be restricted to human placenta and liver suggesting a role in Rab27A-dependent membrane trafficking in specific tissues. C2 domains fold into an 8-standed beta-sandwich that can adopt 2 structural arrangements: Type I and Type II, distinguished by a circular permutation involving their N- and C-terminal beta strands. Many C2 domains are Ca2+-dependent membrane-targeting modules that bind a wide variety of substances including bind phospholipids, inositol polyphosphates, and intracellular proteins. Most C2 domain proteins are either signal transduction enzymes that contain a single C2 domain, such as protein kinase C, or membrane trafficking proteins which contain at least two C2 domains, such as synaptotagmin 1. However, there are a few exceptions to this including RIM isoforms and some splice variants of piccolo/aczonin and intersectin which only have a single C2 domain. C2 domains with a calcium binding region have negatively charged residues, primarily aspartates, that serve as ligands for calcium ions. This cd contains the first C2 repeat, C2A, and has a type-I topology.
Statistics
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PSSM-Id: 176056
View PSSM: cd08521
Aligned: 20 rows
Threshold Bit Score: 130.838
Threshold Setting Gi: 221113744
Created: 5-Feb-2010
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
3FDW_A         10 GRIAFSLKYeqqtqSLVVHVKECHQLayadeakkrSNPYVKTYLLPDKsrqgKRKTSIKRdtvNPLYDETLRYEipesll 89   human
XP_002196139  262 GEIEFALKYnfracILEICIKGCKNLaygeekkkkCNPYVKVYLLPDKspwsKRKTAVKKgtvDPEFDETLKYKiehsql 341  zebra finch
CAG09849      345 GDVVFTLSYnnstnGLLVFIKGCHGLaygdasrhlSNPYVKCYLLPDKtrqsKRKTSIKRntvDPVYNETLKYSikrsql 424  Tetraodon nig...
XP_690816      72 GEIEVVLAYnnrtsCLEITIKACRNLmfrd-tkkkCHPYVNVCLLPKKchnfKMKTAVRKg-kNPVYNETFTCVlsadll 149  zebrafish
CAG09204      285 GELELSLHYnrdtfCLEVTVGAGRNLsygdprkkqCHPYVKLCVLPEKs--sKLKTAVKKhttNPIFNEVLKYPverhll 362  Tetraodon nig...
XP_001203778 1911 GDVSFGLGYnfktnVFEVKIGGCRDLvpidakknkSDPYIKTYLLPDKsknsKRKTRTKKgtiNPNYSENLKYPiaqsel 1990 purple urchin
XP_002124517  109 GSVEFGMKYshktgSLNINIVGCSDLaavd-dkklSNPYVKTYLLPDKq--sKRKTKAKKktiNPVYNETLKYIipesel 185  Ciona intesti...
EEC16277     1224 GEALFAIVYnyksgLLEVHVKECRNLapvdtkrnrSDPYVKVYLLPDKtksgKRKTKVKKhtlNPVFEEVLKFRvtmsel 1303 black-legged ...
XP_002166631   52 GEILFSLKYdslsgIFSVNIEKAKEIaaansklhsSDPYVKVYLLPDKskstKKKTTHKRktlNPQWNETIKYKlskdel 131  green hydra
CAZ32710      128 GEMLMAVIYddaynTLRISIKQARNLaiadrkrniTNPYVKCYLLPDRtkgsKRKTSYKKatcNPVYDEEFKYHilkqdl 207  Schistosoma m...
3FDW_A         90 aqrTLQFSVWHHGRFGrntFLGEAEIqmdswk----ldkklDHCLPL 132  human
XP_002196139  342 rsrQLQVSVWHAGALKyrvFLGEVVIplaawnf--eedsmqFDWYPL 386  zebra finch
CAG09849      425 lnrSVLISVWHYGRLSrntFLGEVEIsldsrd----ldsthLDRMAL 467  Tetraodon nigroviridis
XP_690816     150 ssaVLQLSVWHSRGLKrrlFLGETLIrladmdl-edtdsqrSLCCKL 195  zebrafish
CAG09204      363 igkRLQASVWHSRTLKrktFLGEVLIpldgwrf-ddkafqgFNWYPL 408  Tetraodon nigroviridis
XP_001203778 1991 etrTLSVAIWHYDRFGhnmFLGEVLLpldgld----ltsypVKWHQL 2033 purple urchin
XP_002124517  186 strTLNVAVWHDKVGVn-vFLGEVNLelnfvd---fsdprqSDVYVL 228  Ciona intestinalis
EEC16277     1304 qarTLWLSVWHSDMFGrndFLGEVMLpltydt----lektdVKCYSL 1346 black-legged tick
XP_002166631  132 lekVLQVSIWNHDRFGrndFLGEVQVniqqysktnsldtekSIWYTL 178  green hydra
CAZ32710      208 tlrTLQVSVWHHNALGlnlFLGEIFLpltfh-----qfdqaSHWYTL 249  Schistosoma mansoni
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