Conserved Protein Domain Family
PBP2_LeuO

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cd08466: PBP2_LeuO 
The C-terminal substrate binding domain of LysR-type transcriptional regulator LeuO, an activator of leucine synthesis operon, contains the type 2 periplasmic binding fold.
LeuO, a LysR-type transcriptional regulator, was originally identified as an activator of the leucine synthesis operon (leuABCD). Subsequently, LeuO was found to be not a specific regulator of the leu gene but a global regulator of unrelated various genes. LeuO activates bglGFB (utilization of beta-D-glucoside) and represses cadCBA (lysine decarboxylation) and dsrA (encoding a regulatory small RNA for translational control of rpoS and hns). LeuO also regulates the yjjQ-bglJ operon which coding for a LuxR-type transcription factor. In Salmonella enterica serovar Typhi, LeuO is a positive regulator of ompS1 (encoding an outer membrane), ompS2 (encoding a pathogenicity determinant), and assT, while LeuO represses the expression of OmpX and Tpx. Both osmS1 and osmS2 influence virulence in the mouse model of Salmonella. In Vibrio cholerae, LeuO is involved in control of biofilm formation and in the stringent response. The topology of this substrate-binding domain is most similar to that of the type 2 periplasmic binding proteins (PBP2), which are responsible for the uptake of a variety of substrates such as phosphate, sulfate, polysaccharides, lysine/arginine/ornithine, and histidine. The PBP2 bind their ligand in the cleft between these domains in a manner resembling a Venus flytrap. After binding their specific ligand with high affinity, they can interact with a cognate membrane transport complex comprised of two integral membrane domains and two cytoplasmically located ATPase domains. This interaction triggers the ligand translocation across the cytoplasmic membrane energized by ATP hydrolysis.
Statistics
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PSSM-Id: 176155
View PSSM: cd08466
Aligned: 32 rows
Threshold Bit Score: 192.852
Threshold Setting Gi: 52842611
Created: 21-Oct-2009
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
putativeputative
Feature 1:putative substrate binding pocket [chemical binding site]
Evidence:
  • Comment:based on the sequence-structure comparison with Burkholderia sp. DntR structure (1UTH_A)

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1              # ##   #                                                             #    
CAQ30594     113 VFHLCVCSPLDSILTSQIYNHIEQIAPnIHVMFKSslnq--nteHQLRYQetEFVISYEdfh--rpeFTSVPLFKDEMVL 188 Escherichia col...
YP_269732    100 NFHINILGVGNNDFLTKLSQRLAHVAPnISLTVSTemlv--daeKSLRDKeyDLHLDYLtid--eigCHHQELFNDQLFI 175 Colwellia psych...
NP_937582    107 TFRLSSHKDIDLMLLPPLMDHLAQHAPnVKLCADVehlderdrqADLRTRkvDMILATVpfd--ehgYHNELLFELPLVM 184 Vibrio vulnific...
NP_796729    114 LFKLAICSPCDMRFAPKIMAKINQQAPnVQLHLDAefdr--llsERMRYQeiDFVIDYArfd--eqgFSSTEIFKDELVV 189 Vibrio parahaem...
YP_002313609  93 KFRVSSHKDLDLMLIPSLVRYRQKKAPhTKFIADIehdseesrqDDLRMRkvDIIISTVqle--ehgYHNQLLFKQDLVV 170 Shewanella piez...
NP_230430     99 EFIIGGQGYFDLVVLPPLLQKISDIAPhITVNLKSyeddhftpsQVLSEReaDLFLAALpis--hpsIVTTQVAEEALVV 176 Vibrio cholerae...
YP_001502603  98 HFSLMGPEEYNAKLLSAFAGEQNNGLT-YSLMQQAstde--eaiASLRTRmtDLVIDSVvla--dssIESELLFEDRIVL 172 Shewanella peal...
YP_001250875  97 FLKTASVGTGEYFILPRLCAHLAKNAPkFVLKISSlsey--lslEYLLMSelDFAIGPGfvet-gnnIIRELLFEEEAVC 173 Legionella pneu...
YP_096410    100 SFKIALPDYVELILLPSLMAALEKLPH-IVIETTIqsma--ddpHLFIDEnvDLAIGAInenslsaqLKVEKLYEEEIIC 176 Legionella pneu...
AAC36989      97 TFNVSFAGTLGDALFDRFLLRVKRLAPgLQVRLTEsssw-eadvAAMRSNelDLAFSPFptrh-peiVEEVVTSFNMWVC 174 Ralstonia solan...
Feature 1                                                #                                       
CAQ30594     189 VASKNHPTIkgpl--lkhdVYNEQHAAVs-------lDRFASFSQPWYdtvdk--qasiAYQGMAMMSVLSVVSQtHLVA 257 Escherichia col...
YP_269732    176 IARKGHPNLnnktnlllseYLAEKHAVLa-------pRKGNVYPLSLAlqdfs-ynreiKYTSTSIENILEIVSAtDLIC 247 Colwellia psych...
NP_937582    185 VCRQDHPRIqnti--thqqFFAEQHLLWe-------tKRQNQDIVDSLalepq-pqrniAYKTGSSCNAIMMAAQtDWIC 254 Vibrio vulnific...
NP_796729    190 IASKTHPRIqggv--saeqLINEKHAKLsk------vHGQRSFSEQAYreld----cqaAYEGSSLSNLLYVVSQsELVT 257 Vibrio parahaem...
YP_002313609 171 ALSSEHPRIqgrm--sedaFFAEEHILWqt------kRMDRYTLNSVAtkhlp--srrvAYSTGSAMTGLNLVADtEWLC 240 Shewanella piez...
NP_230430    177 VYAKNHPRLgstl--sfeqFFAEKHTALt-------sRRFGNYLFSSLvdqvl-parrvHYQSESMLNLMATASVtDLLC 246 Vibrio cholerae...
YP_001502603 173 IAAKSNSTIdenl--tfeqYQELPQSVLs-------lRRSGKLALEMFledklevrrniSHEASSIMANILIVSQtMLFC 243 Shewanella peal...
YP_001250875 174 VMHKSHPLAnqel--tqdmYLQAEHVDIqf------sYMGNENILYKAlqgyh--qrniKVIVPNIISALEVVYHtNFIT 243 Legionella pneu...
YP_096410    177 FASKNNPLInrpl--sledYISNKHIAFs-------fNQERYSHSAEYlykrk-lvrndMLFVKNILPAIFAVANsDAVI 246 Legionella pneu...
AAC36989     175 ARKDHPVLKdrcs---ldqYLECEHIFIaqgnpgsraAPSLIPLDYALqqrgl--krhsTMTVHAWRTQAEVAAQtDLIF 249 Ralstonia solan...
Feature 1                                       #                        
CAQ30594     258 IAP-RWLAEEfaeslELQVLPLPLKQN-SRTCYLSWHEaagrDKGHQWMEEQLVSI 311 Escherichia coli BL21(DE3)
YP_269732    248 IMP-GTVLQSmhnvnDYIWFNPPFKTK-QMIAYMNWHWsmehVKSHRWLRTIIIDI 301 Colwellia psychrerythraea 34H
NP_937582    255 VTG-QWHADLmakahQLQVMPLPFKLN-KLPIYMTWHHsqqqDSGHQWLKSALMQA 308 Vibrio vulnificus YJ016
NP_796729    258 IAP-RWMAENavnsdQLQILDFPFANK-EISGFLSWHEssekDKGHIWLRDQLMVT 311 Vibrio parahaemolyticus RIMD 2210633
YP_002313609 241 VSS-RWHSNQvavtgRIQVLELPFATQ-QVPVYMTWHHsqhkDAGHQWFREAIMTT 294 Shewanella piezotolerans WP3
NP_230430    247 FTP-KRLADMwaeklGLQIQPLPFEIR-SVPTFMCWHKakqqDKGLIWLREQIEAI 300 Vibrio cholerae O1 biovar eltor str. N1...
YP_001502603 244 HVP-LRLAIHyqeqlSLKIIEPPISLK-PVPVIMQWHKsnsiDPSHIWLRNRIKQI 297 Shewanella pealeana ATCC 700345
YP_001250875 244 TVP-RTLAVTfkdryQIELHPLPFPKK-TFLVNFYYHKrlsnHKPLQWVLDVIKKY 297 Legionella pneumophila str. Corby
YP_096410    247 STApSFLINMfarqfNLGMQPLPFNLE-PTPIYLVTHSksqyDEGVSWLAKLVSSS 301 Legionella pneumophila subsp. pneumophi...
AAC36989     250 TVN-SLMKDLvceayNLNAFPLPSELEtVLGLNMLWHRsrntHPMLVWARNLFKQV 304 Ralstonia solanacearum

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