Conserved Protein Domain Family
PBP2_HcaR

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cd08450: PBP2_HcaR 
The C-terminal substrate binding domain of LysR-type transcriptional regulator HcaR in involved in 3-phenylpropionic acid catabolism, contains the type2 periplasmic binding fold.
HcaR, a member of the LysR family of transcriptional regulators, controls the expression of the hcA1, A2, B, C, and D operon, encoding for the 3-phenylpropionate dioxygenase complex and 3-phenylpropionate-2',3'-dihydrodiol dehydrogenase, that oxidizes 3-phenylpropionate to 3-(2,3-dihydroxyphenyl) propionate. Dioxygenases play an important role in protecting the cell against the toxic effects of dioxygen. The expression of hcaR is negatively auto-regulated, as for other members of the LysR family, and is strongly repressed in the presence of glucose. This substrate-binding domain shows significant homology to the type 2 periplasmic binding proteins (PBP2), which are responsible for the uptake of a variety of substrates such as phosphate, sulfate, polysaccharides, lysine/arginine/ornithine, and histidine. The PBP2 bind their ligand in the cleft between these domains in a manner resembling a Venus flytrap. After binding their specific ligand with high affinity, they can interact with a cognate membrane transport complex comprised of two integral membrane domains and two cytoplasmically located ATPase domains. This interaction triggers the ligand translocation across the cytoplasmic membrane energized by ATP hydrolysis.
Statistics
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PSSM-Id: 176141
View PSSM: cd08450
Aligned: 7 rows
Threshold Bit Score: 294.67
Threshold Setting Gi: 257760314
Created: 13-Oct-2009
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
putativeputative
Feature 1:putative substrate binding pocket [chemical binding site]
Evidence:
  • Comment:based on sequeuce similarity to CatM from Acinetobacter baylyi ADP1

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1           #                                                #                        
BAI31811   91 QLTIGFVPSaevNLLPKVLPMFRLRQPdTLIELVSlittqQEEKIRRGeLDVGLMRHpvy-speIDYLELFDePLVVVLP 169 Escherichia coli O...
YP_593654  94 KFVIGFLLGqeaIWLSESLRILREEARdVEITLMTksspeLAVGLMQGkIDVALLRRevn-tvgLAFRFLSKePLIAILP 172 Acidobacteria bact...
YP_045207  92 CLRIGFVPVaevQVFPHVLPNFRAHHPeLRIELHSlnndqQLKALKRGeLDLCFIRDniqhrdgIHSQLVLSePLIFILP 171 Acinetobacter sp. ...
NP_929461  91 VLTIGFVPSaevKVLPEVIPIFRLAHPgAKVELISlfnpgQEEKLLQGdIDVAFMRPpik-sdsIDFKVILNePLVVLMP 169 Photorhabdus lumin...
NP_774195  93 VLSMGFLVGlevMWLPQLLRILREEAPdVDVTLSTqsspeLALALMRGkLDIAFLRPekd-negVVFKILAKePLIAVLP 171 Bradyrhizobium jap...
YP_554747  93 VFALGFLTGqemTWLPRAMQVLREELPnIDVTVSShyspdLADALARGkLDLAFLRAepg--fdLDYQVVSSeKLIVLMP 170 Burkholderia xenov...
NP_107445  93 SFALGFLTGqeiDWLPEAMRILHDELPnLEVIVSSqfspqLANALTRGkLDVAFMRPeaq-sadLTYRLITKePLVAVLP 171 Mesorhizobium loti...
Feature 1                            ##      #                                                
BAI31811  170 VDHPLahekeitaaqLDGVNFVSTDpaysGSLAPIVKAWFaqeNSQPniVQVATNilVTMNLVGMGlGVTLIPGYmnnfn 249 Escherichia coli O...
YP_593654 173 TRHRLaglrtvppqdLARESFISTAra-aPVLKSVIDHYAaktGITLneHYDAETlsGGMSLVASTgGFTLLPLYaqnal 251 Acidobacteria bact...
YP_045207 172 KQHPLakleripleaLNNIDFIIPAadqaRVLNQHILQFAkqhQIQFniIQSADTvlSNITSVALNlGCSILPNYvstll 251 Acinetobacter sp. ...
NP_929461 170 ANHRLrycekisvndLQGENFITPDptqsGVLQQIIENYFtehNVVPniIQTANNmlLILNMVSLNlGCAIVPFYitsim 249 Photorhabdus lumin...
NP_774195 172 AEHRLasrkkirpqdLAREIYVSSArt-sPVLQEVIHNYAsrvGITLkaKYEGENisSAMSLVASTgGISLVPLYaqnml 250 Bradyrhizobium jap...
YP_554747 171 SDHRLtgrsairpedFAGETFIMASnk-aRVLHDVIQRYLddnGIAVtpGHGVDNlaMAMSLVASTrGLALMPEYannll 249 Burkholderia xenov...
NP_107445 172 SDHRLaerdsidpqdIVGETFISVSnt-aPTLRVVIEEYLersNLGIrpDHEVDNlaMAMSLVASTrGVAFLPAYaksfm 250 Mesorhizobium loti...
Feature 1                                           
BAI31811  250 tgQVVFRPIAgnvpSIALLMAWKKgemkpaLRDFIAIV 287 Escherichia coli O103:H2 str. 12009
YP_593654 252 ipSVVARPLRgeipTIGLMMGYNKsntsplLKRFLLRA 289 Acidobacteria bacterium Ellin345
YP_045207 252 peNCVMRPLDielpHFDLFMAYQSasktppVTTFIKAL 289 Acinetobacter sp. ADP1
NP_929461 250 tdAVICRPLSgyspTIELIMAWRKdntselLLHLIDVV 287 Photorhabdus luminescens subsp. laumondii TTO1
NP_774195 251 apNVVARALEggtpTVDLALGYNRtnpsplLRRLLSRA 288 Bradyrhizobium japonicum USDA 110
YP_554747 250 pwSVVSRPLEgetpTIDLVIGYSRsntspvLKLFLSRA 287 Burkholderia xenovorans LB400
NP_107445 251 pwSVISRPISgdtpTIDLVIGYNKanasptLKLFLSRI 288 Mesorhizobium loti MAFF303099

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