Conserved Protein Domain Family
PBP2_GltC_like

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cd08434: PBP2_GltC_like 
The substrate binding domain of LysR-type transcriptional regulator GltC, which activates gltA expression of glutamate synthase operon, contains type 2 periplasmic binding fold.
GltC, a member of the LysR family of bacterial transcriptional factors, activates the expression of gltA gene of glutamate synthase operon and is essential for cell growth in the absence of glutamate. Glutamate synthase is a heterodimeric protein that encoded by gltA and gltB, whose expression is subject to nutritional regulation. GltC also negatively auto-regulates its own expression. This substrate-binding domain has strong homology to the type 2 periplasmic binding proteins (PBP2), which are responsible for the uptake of a variety of substrates such as phosphate, sulfate, polysaccharides, lysine/arginine/ornithine, and histidine. The PBP2 bind their ligand in the cleft between these domains in a manner resembling a Venus flytrap. After binding their specific ligand with high affinity, they can interact with a cognate membrane transport complex comprised of two integral membrane domains and two cytoplasmically located ATPase domains. This interaction triggers the ligand translocation across the cytoplasmic membrane energized by ATP hydrolysis.
Statistics
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PSSM-Id: 176125
View PSSM: cd08434
Aligned: 43 rows
Threshold Bit Score: 215.093
Threshold Setting Gi: 258512471
Created: 21-Oct-2009
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
putative
Feature 1:putative dimerization interface [polypeptide binding site]
Evidence:
  • Comment:based on sequence similarity to the known structures of LysR-type transcriptional regulators.
  • Comment:Most members of the LysR family of transcriptional regulators are tetrameric, while CrgA assembles into octameric rings

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                       ## ##  #   #   #######                                           
P20668        92 TVKIGFptslasQLLPTVISAFKEEYPh--VEFLLRQgsyk--fliEAVRNRdIDLALLGPvptn--fsdITGKILFTeK 165 Bacillus subtilis
NP_786710     91 PIRIAAlrtlsiKWLPDIAQRFLQLSPhaaVQFQFNTdtglspdilNGLRNNkYDVAFCSKmdh---fsdIEFFPVAEqT 167 Lactobacillus p...
YP_001394396  92 HVDIAYifplsqYYIPKTVRSFLNLEEnrnVTFTFKQgitd--ellEGLKNDeYDLIFASYved---epdIIFIPIFDqK 166 Clostridium klu...
NP_465757     91 QIDLAFlqtlgtSLIPKLVQEFLQTEPekqIDFIFHTgvsi--diiQGLKEKkFDIGICSKlen---ernIHFTPIAKqE 165 Listeria monocy...
YP_001089222  91 QIDLAFlrtlgtDFIPDIVHKFLKSNPaksIDFKFHTgvtt--diiQGLKERkYDIAFCSKlek---ekgIEFIPVAKqD 165 Clostridium dif...
YP_812224     87 HIRVGFtm--gqDLIPQLIYKFKQEKDtkdITFSFVQgttd--dlvDQLVNDnLDLVVSPApdlpgledkVDISHLVDqE 162 Lactobacillus d...
YP_002381315  92 NIRIAFiytmgeYVVPQLINKFSLSPAysdVTFSFTQgtsl--tllQDLKAGkTDLAICSYiad---epdINFIPVIQqE 166 Escherichia fer...
YP_001310666  92 TVSISSlytigtHFIPSIISEFLKESPn--TKFEFGIqstf--nilKGLKDGkFDFGFYDEfedikryeeIESVPIKNeE 167 Clostridium bei...
YP_002930926  91 TIRLGLvrplgiSYVPRMAAAFIKKNPkldIDFTFHTdvtg--rllDDMQLGkYDLLFCSKpse---eyhFTAEPVMKqR 165 Eubacterium eli...
NP_464016     92 NIDLGFiytmgaHTVPELVQNFTKVEShkdITFSFFQgatk--siiPDLKNEkFDLAICSYven---epdIEFLPLTKqE 166 Listeria monocy...
Feature 1                                                               ######      ## ##        
P20668       166 IYALVPLNHPLakqk--tvhLIDLRNDQFVLFpegfvlremaidtckqaGFAPLVSTEGEDld-aIKGLVSAGmGVTLLP 242 Bacillus subtilis
NP_786710    168 MVCITPTTHPLanrs--sinLAETLAYPQITFsqrsglhpimqklfndcGGQPISAYAVEEdq-aIAGLVASGfGIAVVP 244 Lactobacillus p...
YP_001394396 167 LFVIVPLDHPLaeld--svdLKDIEPYPLVGYdkasglgkltskllknmDLHPKIICEASDey-aLSALVAANfGVSIIA 243 Clostridium klu...
NP_465757    166 LVLIVPKSHPLaekd--widLIETVDYPHIAFskksglrpiidelfrkiGADYQIAYEIEVdq-vIAGMVAQNfGIAVVP 242 Listeria monocy...
YP_001089222 166 LVLIVPYSHPLaakd--tidLKETIPYPQIVFnqrsglryiiddmfkkiNQQPNIVYEVEEdq-vIAGLVAKNfGIAVVP 242 Clostridium dif...
YP_812224    163 MLAVVPFSNPLaqkd--svsLADLAKYPMIYYskssrlrplldkmfaeaVVKPKIIMESMEdh-tIIGFVHWGyGVAIVP 239 Lactobacillus d...
YP_002381315 167 LVVVTAREHPLaslyknevdLGETIHYPYVYFsensglrpfidnvfmqqKLVPDIACYVEEdt-aMAGLVSINyGIAIMP 245 Escherichia fer...
YP_001310666 168 LVLIVPKNHHLadkt--evfLKGLKEESFVFFsegikgkm--csifesiGFVPKTSIEYNEssmvVTGFVSAGlGISIVP 243 Clostridium bei...
YP_002930926 166 LVLITPKGHPLakkrkrsinLAETAGYSYVCFdknsgirsifdemlkrsDITVKVAYETEEdq-vIAGLVASGfGIAVVP 244 Eubacterium eli...
NP_464016    167 LVVVVAENHPLakyd--sidLQDTADYSYIFFsdtsglrplidslfaeiNIQPKIGCYVEEdt-aMVGLVSVDyGISIMP 243 Listeria monocy...
Feature 1                                                       
P20668       243 estfaettprFTVKIpiefpqvKRTVGIIKPKnrelapsANDFYEFV 289 Bacillus subtilis
NP_786710    245 nmsi--lqtmPVKIIplsfptwQRILYMGTLKhrtpqpaVEEFITFV 289 Lactobacillus plantarum WCFS1
YP_001394396 244 eapa--lkyaKVKKLhiknikySRQIHLAYKKnkyfapaVHNFISYV 288 Clostridium kluyveri DSM 555
NP_465757    243 nmpi--lnyvDVKVLpirspnwERFFYLAVAKnqtltpaAEKFKQFM 287 Listeria monocytogenes EGD-e
YP_001089222 243 nmnm--lsftKVKVIqiihpswERNFYLAFIKdrylppaIKNFKNFV 287 Clostridium difficile 630
YP_812224    240 hlpq--ldprTVKLLhidenlgVHPIFVVKKSdhfltpaVTRFEQFI 284 Lactobacillus delbrueckii subsp. bulgaricus ATCC...
YP_002381315 246 rita--lsyyPVHILniknaipPRYIYLATMKdrvlspaLQTFKDVI 290 Escherichia fergusonii ATCC 35469
YP_001310666 244 ntpn--liteEVSVLkikepncYRTVHMGWLKngymtpsAKIFKDFV 288 Clostridium beijerinckii NCIMB 8052
YP_002930926 245 ymdi--lekmSVEIFeiespeyERSFYMVNDNstymspaVETFRNFV 289 Eubacterium eligens ATCC 27750
NP_464016    244 kiss--lahyNVKVLsinepkhDRFIYLASLKnhyispaSKAFKDFA 288 Listeria monocytogenes EGD-e

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