Conserved Protein Domain Family
PBP2_IlvY

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cd08430: PBP2_IlvY 
The C-terminal substrate binding of LysR-type transcriptional regulator IlvY, which activates the expression of ilvC gene that encoding acetohydroxy acid isomeroreductase for the biosynthesis of branched amino acids; contains the type 2 periplasmic binding fold.
In Escherichia coli, IlvY is required for the regulation of ilvC gene expression that encodes acetohydroxy acid isomeroreductase (AHIR), a key enzyme in the biosynthesis of branched-chain amino acids (isoleucine, valine, and leucine). The ilvGMEDA operon genes encode remaining enzyme activities required for the biosynthesis of these amino acids. Activation of ilvC transcription by IlvY requires the additional binding of a co-inducer molecule (either alpha-acetolactate or alpha-acetohydoxybutyrate, the substrates for AHIR) to a preformed complex of IlvY protein-DNA. Like many other LysR-family members, IlvY negatively auto-regulates the transcription of its own divergently transcribed ilvY gene in an inducer-independent manner. This substrate-binding domain has significant homology to the type 2 periplasmic binding proteins (PBP2), which are responsible for the uptake of a variety of substrates such as phosphate, sulfate, polysaccharides, lysine/arginine/ornithine, and histidine. The PBP2 bind their ligand in the cleft between these domains in a manner resembling a Venus flytrap. After binding their specific ligand with high affinity, they can interact with a cognate membrane transport complex comprised of two integral membrane domains and two cytoplasmically located ATPase domains. This interaction triggers the ligand translocation across the cytoplasmic membrane energized by ATP hydrolysis.
Statistics
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PSSM-Id: 176121
View PSSM: cd08430
Aligned: 25 rows
Threshold Bit Score: 282.931
Threshold Setting Gi: 254517185
Created: 19-Oct-2009
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
putative
Feature 1:putative dimerization interface [polypeptide binding site]
Evidence:
  • Comment:based on sequence similarity to the known structures of LysR-type transcriptional regulators.
  • Comment:Most members of the LysR family of transcriptional regulators are tetrameric, while CrgA assembles into octameric rings

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                              ## ##  #   # #######                                             
AAA67576             92 ELHIFCSVTAaYSHLPPILDRFRAEHPsVEIKLTTGDaadamEKVVTGeaDLAIAGKPetlpgaVAFSMLENlAVVLIAP 171 Escheric...
goetting:HRM2_21080  91 EISLYCSVTAaYSILPDIIARYRVIHPkVQIKLETGDpaksiALIVNRdvDVVIAALPekiphnLSFLKMITsPLVFIAP 170 Desulfob...
NP_760027            92 EIRLFCSVTAsYSHLPELLSEFRLQHPfIEFKISTGDpaqamDKILNGeaDIAISAKPdtvpakVAFEPISEiPLSVIAP 171 Vibrio v...
YP_064461            91 EISLYCSVTAvLSILPTIFRRFKNEHPeVRIRIETGDaaeaqQKINAKeaDISIIALPdkqpenLKFIEFLQtPLIFILP 170 Desulfot...
ZP_01223050          92 KLKMFCSVTAsYSHLPSILNEFRLTYPqIEIQLRTGDpaeaiDKVISGevDIAIAAKPetmpskLTYIELDQvQMSVISP 171 Photobac...
YP_002139557        110 TLSIYASITAvYSLLPELLESYREKYPeVQLELRTGAaeqavTQVQNGeiDLAVAALPdrhssnLEFLPIATiPLIFIAP 189 Geobacte...
ZP_01224864          92 SLKLYCSVTAsYSYLPPLLEKFRQAHPnIDINLDTGDaadgiDQIKNQkvDLAIAARPdelssrIHFQTIATvPLAIAAP 171 gamma pr...
ZP_01313518          91 NLSIYASITAvYSILPGVLERYRQRYPdVQIHLHSGAaeqalDQVNSGeiDIAVAALPagkrsqMEFLPLTTtPLVFIAP 170 Desulfur...
YP_002128304         92 ELSVFCSVTAsQSHLPAMLRQFRQQHPgVDIRLITGDpalaiEKVKGKqcDLSIAIYTpdmptdLHFTALDNvPLVLIAP 171 Alteromo...
ZP_05129243          91 ELRIFCSVTAtHRLLSPLLAAYREAYPqVHILLQTGDqadgiERVRRGssDVSVIARPeslpasFAFSPLAStPLCLCLP 170 gamma pr...
Feature 1                                                                    ######     ## ##           
AAA67576            172 alpcpvrn-----qvsvekpdWSTVPFIMADqGPVRRRIELWFrrnkisnPMIYATVGGHEAMVSMVALGcGVALLPeVV 246 Escheric...
goetting:HRM2_21080 171 krfpsiiq------yrngridWEKTPMILPDvGLSRERMDLWFarh-qqvPNIYSRVAGHEGIIALVNLGcGIGLVPeLV 243 Desulfob...
NP_760027           172 qgvssftd-----emnrdkpdWSSIPYIVPEaGTARERANTWFkkm-kikPNIYAQVSGHEAIVSMVALGcGIGIAPdVV 245 Vibrio v...
YP_064461           171 nqfteaii------vgeegidWQKTPIIMPNrGLSRQRTDKWFaek-nihPNIYSQVAGNEAIIAMVSMGcGAGVVPqLV 243 Desulfot...
ZP_01223050         172 liappaiq-----kyltgdpdWQSLPFILPEsGPARSRADKWFktk-kiqPSIYAQISGHEAIVSMVALGcGIGIAPdVV 245 Photobac...
YP_002139557        190 rhaiaadv-----pqeggeldLSRAPLVLPQtGLSRRRLDQWLkeh-risPNITSEVSGNEAIIAMVRLGcGVGIVPqLV 263 Geobacte...
ZP_01224864         172 tincavkk-----alqaetidWANLPIILPEhGVARKRFEQWFrqktsvrPNVYAQVSGQEALVSMVALGcGIGIAPnVV 246 gamma pr...
ZP_01313518         171 anvdpled-----prcnglldLSRVNLVVPQyGLSRDRLNQWLkte-rvaANIRSEVSGNEALIALVRIGgGIGIVPkLV 244 Desulfur...
YP_002128304        172 rewrlt---------qlsqldWTKHQVIMPEtGPTRRTAYHWFaeh-girPNVYAYVGGNEAIVSMVALEcGVGFVPkVV 241 Alteromo...
ZP_05129243         171 evecdlrdtvkdlkgralwsaLEDVPWVLPErGVSKELIDRWLqrrfltpPPIYARVAGHEAIVAMVSLGlGVAILPrLV 250 gamma pr...
Feature 1                                                              
AAA67576            247 LENSPepvrnRVMILersdektPFELGVCAQKkrlhePLIEAFWKIL 293 Escherichia coli str. K-12 substr. MG1655
goetting:HRM2_21080 244 LEKSPlm--dDMMILndlpelpPYEIGLCTIEknlsrPMIRALWDLA 288 Desulfobacterium autotrophicum HRM2
NP_760027           246 INNSPvk--eKIQRLkva-pikPFELGVCCIKsqlenPLIQALWKVA 289 Vibrio vulnificus CMCP6
YP_064461           244 MEKSPla--sQVTVTevyprltPFSVGACVTTkklkePIVDAFWQVA 288 Desulfotalea psychrophila LSv54
ZP_01223050         246 INNSPms--nKVQRLshe-siePLSLGLCCKQsrekePLLHALLQLF 289 Photobacterium profundum 3TCK
YP_002139557        264 LERSPfr--dEVAVLekapslkPYEVGLCCGRrnlqrPSVKAFWQLA 308 Geobacter bemidjiensis Bem
ZP_01224864         247 VENSPvk--dRIELLpgt-liaPFDLGLCCFKkklndPLIAEFMSMA 290 gamma proteobacterium HTCC2207
ZP_01313518         245 LQRSPfs--sEVRIIekapqldPYEVGLCCNRsalqlPSVRAFWKMA 289 Desulfuromonas acetoxidans DSM 684
YP_002128304        242 LDHSSm---aNAVTQiqvediePYALGLCCLQdkqnePLINAFMQLN 285 Alteromonas macleodii 'Deep ecotype'
ZP_05129243         251 VDASGla--gSLRVLtlkedlpPFEIGLCTRQrrrldPVIAGLWQVA 295 gamma proteobacterium NOR5-3

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