Conserved Protein Domain Family
PBP2_IciA_ArgP

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cd08428: PBP2_IciA_ArgP 
The C-terminal substrate binding domain of LysR-type transcriptional regulator, ArgP (IciA), for arginine exporter (ArgO); contains the type 2 periplasmic binding fold.
The inhibitor of chromosomal replication (iciA) protein encoded by Mycobacterium tuberculosis, which is implicated in chromosome replication initiation in vitro, has been identified as arginine permease (ArgP), a LysR-type transcriptional regulator for arginine outward transport, based on the same amino sequence and similar DNA binding targets. Arp has been shown to regulate various targets including DnaA (replication), ArgO (arginine export), dapB (lysine biosynthesis), and gdhA (glutamate biosynthesis). With abundant nutrition, ArgP activates the DnaA gene (to increase replication) and the ArgO (to export redundant molecules). However, when nutrition supply is limited, it is suggested that ArgP might function as an inhibitor of chromosome replication in order to slow replication. This substrate-binding domain has significant homology to the type 2 periplasmic binding proteins (PBP2), which are responsible for the uptake of a variety of substrates such as phosphate, sulfate, polysaccharides, lysine/arginine/ornithine, and histidine. The PBP2 bind their ligand in the cleft between these domains in a manner resembling a Venus flytrap. After binding their specific ligand with high affinity, they can interact with a cognate membrane transport complex comprised of two integral membrane domains and two cytoplasmically located ATPase domains. This interaction triggers the ligand translocation across the cytoplasmic membrane energized by ATP hydrolysis.
Statistics
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PSSM-Id: 176119
View PSSM: cd08428
Aligned: 21 rows
Threshold Bit Score: 291.845
Threshold Setting Gi: 218887213
Created: 19-Oct-2009
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
putative
Feature 1:putative dimerization interface [polypeptide binding site]
Evidence:
  • Comment:based on sequence similarity to the known structures of LysR-type transcriptional regulators.
  • Comment:Most members of the LysR family of transcriptional regulators are tetrameric, while CrgA assembles into octameric rings

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                              ## ##  #   #  #######                                            
CAQ33227             92 LLSLAVNaDSLATWLLPALAPVLADSPirLNLQVEDetrtqERLRRGEVVGAVsiqhqalpSCLVDKLGAlDYLFVSSKP 171 Escheric...
goetting:HRM2_09790  92 TMSVGVNaDSLATWFLNAIHSFLIEEQvlLDIRVDDqeqthQLLRDGEVMGCIttleqpmqGCRIDPIGRmTYRMVATPG 171 Desulfob...
YP_002434160         92 TLALGINgDSLSTWFLPAVRDFLIRERllLDLRGDDqdethKMLRDGEVIGCIsvqdrpmqGCLMEYLGRmDYRLIASPE 171 Desulfat...
YP_002436534        109 TLPVAVNaDSLATWFPDAVAPFLRAERalLDLSVDDqerthLLLRDGHVAAAVstraaavqGCRCLPLGRvDYLCLAAPH 188 Desulfov...
YP_009816            92 TLPVAINaDSLATWFLDAVGPLLRRERvlLDIVTEDqehtyRLLREGRVAACIttradpvqGWRSLALGDmPSLCLATPE 171 Desulfov...
YP_001173164         92 VVVAALNaDSLGTWFFPALAEVLIRERvlLDLTVEDqdhtySLLETGLAIGCIstepkpmrGCSASPLGSmRYRLVASSE 171 Pseudomo...
YP_389962           106 QLSVAVNaDSLATWFLEAVDAVAEQEKllLDVLVDDqdrthDLLRNGEVVGCVstratpfhGCSMQRLGSmEYLVCAAPD 185 Desulfov...
NP_718438            92 TVRIAVNaDSLATWFLPALADMFSRHAwlLELIVDDesythHLLKNGEAVGCVtttanpmaGCSSEFLGTmEYMCVATPQ 171 Shewanel...
YP_001583288         94 SVTIALNsDSLGTWFLPALTSVLAGERilFELIVEDqdhtfALLESGMAVGCVttepkpmrGCVATPLGTmRYRLLAADA 173 Burkhold...
YP_002794799         92 PLPVGVNaDSLGTWLLPAVAKVVWRERfvLQAIVDDeahthDLLRSGEVVGCVtshpqpptGCISQPLGQmRYRFYATPD 171 Laribact...
Feature 1                                                                             ######     ## ##  
CAQ33227            172 FAEKYFPNGvtrs---------allKAPVVAFDHLDdmh------qaFLQQNFdlpp-gsvpCHIVNSSEaFVQLARQGt 235 Escheric...
goetting:HRM2_09790 172 FADRWFPNGitme---------aasRAPAVTFNRKDtlh------qqLLTQAFekcp-kympIHYVPSAEkFADFIASGl 235 Desulfob...
YP_002434160        172 YAAKWFPEGmnle---------sasKAPFFIFNRKDhlh------ykMFPLALgevp-tplpAHYLPSSEqFVSFIEAGl 235 Desulfat...
YP_002436534        189 FAAQWFPAGltea---------alcRAPAVIFNRADevhhqflrglpPGATSFdria-rslpLHYIPSSErFVDVVAQGl 258 Desulfov...
YP_009816           172 FAARWFSEGltpa---------avrEAPAVLYNRKDsl-------hhRFLSDLfeee-vdfpAHYVPSSErFVDVLAGGf 234 Desulfov...
YP_001173164        172 FRQKHFANGltrn---------aarKAPVVAYTRKDtlq------ssFLLRRFglpe-gaypCHYVPGAEpHFSAIRYGl 235 Pseudomo...
YP_389962           186 FAAEHGLRVgadgraa---taadllRAPAVLYNRVDgvh------dtMLQTLYgiapdtpypRHYVPSSEaFVKMVAAGf 256 Desulfov...
NP_718438           172 FAARYFEWSdtsndsgsqltqaqltKAPAVVFSTKDklh------ekYLAQYFqmtp-grwwQHTIPSSEsFLEAINLSl 244 Shewanel...
YP_001583288        174 FAARWFPRGltrt---------sarDAPVVAYSRRDtlq------ssFLEQRFglpp-gaypCHYVPGTHaHFAAVRHGl 237 Burkhold...
YP_002794799        172 FVRNHLPDGltre---------avlATPGIAFNRKDslh------fgQMEARFglrl-gdypYLTMPSTHaLFEAVKAGl 235 Laribact...
Feature 1                                                                   
CAQ33227            236 tCCMIPhlqi-ekeLASGELIDLtpglFQRRMLYWHRFapesrmMRKVTDAL 286 Escherichia coli BL21(DE3)
goetting:HRM2_09790 236 aYGMLPeqqi-mplAESGGIIDLtpgySVAVNLYWQCWnlnatlLKKLSEQL 286 Desulfobacterium autotrophicum HRM2
YP_002434160        236 gYGMLPdqqs-kelLEAGLLVDLapshIVPVHLYWHCWnlssalLERFTRHL 286 Desulfatibacillum alkenivorans AK-01
YP_002436534        259 aYGMIPlpqa-rqhLADGRLIDLapghAVPIRLYWHCWglrtrlLDALTHHV 309 Desulfovibrio vulgaris str. 'Miyazak...
YP_009816           235 aYGIVPalqa-erhLADGVLCDLapgrAVSVHLYWHCWslttrfVEELTENV 285 Desulfovibrio vulgaris subsp. vulgar...
YP_001173164        236 gYGMVPelll-heaLRAGEVVDLaadePLEIALYWHTWkvqsprMEHLSRQI 286 Pseudomonas stutzeri A1501
YP_389962           257 gWGCVPhlqa-eplLAGGELVDLapgwALPVALHWHWWdlgtglFKRMAAVL 307 Desulfovibrio desulfuricans subsp. d...
NP_718438           245 gYGLVGhlqa-kplIDQGVLIELtpekRMRVPLYWQHWnikakqTTLVYRAL 295 Shewanella oneidensis MR-1
YP_001583288        238 gYAMVPepligdapLAAQQLVDLapahPTDVTLYWHAWtvqsptMASLSARV 289 Burkholderia multivorans ATCC 17616
YP_002794799        236 gYGLSPelqv-geaLASGEVIDLlpddPPSVSLFWHGWalqsprLARFTATL 286 Laribacter hongkongensis HLHK9

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