Conserved Protein Domain Family
PBP2_CysL_like

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cd08420: PBP2_CysL_like 
C-terminal substrate binding domain of LysR-type transcriptional regulator CysL, which activates the transcription of the cysJI operon encoding sulfite reductase, contains the type 2 periplasmic binding fold.
CysL, also known as YwfK, is a regular of sulfur metabolism in Bacillus subtilis. Sulfur is required for the synthesis of proteins and essential cofactors in all living organism. Sulfur can be assimilated either from inorganic sources (sulfate and thiosulfate), or from organic sources (sulfate esters, sulfamates, and sulfonates). CysL activates the transcription of the cysJI operon encoding sulfite reductase, which reduces sulfite to sulfide. Both cysL mutant and cysJI mutant are unable to grow using sulfate or sulfite as the sulfur source. Like other LysR-type regulators, CysL also negatively regulates its own transcription. In Escherichia coli, three LysR-type activators are involved in the regulation of sulfur metabolism: CysB, Cbl and MetR. The topology of this substrate-binding domain is most similar to that of the type 2 periplasmic binding proteins (PBP2), which are responsible for the uptake of a variety of substrates such as phosphate, sulfate, polysaccharides, lysine/arginine/ornithine, and histidine. The PBP2 bind their ligand in the cleft between these domains in a manner resembling a Venus flytrap. After binding their specific ligand with high affinity, they can interact with a cognate membrane transport complex comprised of two integral membrane domains and two cytoplasmically located ATPase domains. This interaction triggers the ligand translocation across the cytoplasmic membrane energized by ATP hydrolysis.
Statistics
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PSSM-Id: 176112
View PSSM: cd08420
Aligned: 116 rows
Threshold Bit Score: 205.033
Threshold Setting Gi: 33593764
Created: 20-Oct-2009
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
putative
Feature 1:putative dimerization interface [polypeptide binding site]
Evidence:
  • Comment:based on sequence similarity to the known structures of LysR-type transcriptional regulators.
  • Comment:Most members of the LysR family of transcriptional regulators are tetrameric, while CrgA assembles into octameric rings

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                       ## ##  #   # #######                                             
P39647        92 ELKIAAsfTIGEYILPPLLAQLQKLYPeLNLDVMIgnteevsERVRMLqaDIGLIEghtn---eneLEIEPFMEDEMCIA 168 Bacillus subtilis
YP_827874     92 RLALGAstTIAQYLLPRLAGEFLAAFPaIQFSIISgntsdivAALADGriGLGLIEgpag---rsdVKCEPFVEDEIVLV 168 Solibacter usit...
NP_487993     97 TLIVGAsqTTGTYLLPKMIGMFRQKYPdVAVQLHVhstrrtaWSVANGqvDLAIIGgeipgeltesLEIIPYAEDELALI 176 Nostoc sp. PCC ...
NP_053815     97 TLIIGAsqTTGTYLMPRLIGLFRQRYPqVAVQLQVhstrlisWSVANGqvDLAIIGgevptelqdvLQVTSYAEDELALI 176 Porphyra purpurea
NP_050683     97 TLIIGAsqTTGTYLMPRLIGLFRHKYPqIAVQLQVhstrrvaWSVANGqvNLAVVGgkvpdelrskLQITPYVDDELALI 176 Guillardia theta
YP_475803     98 HLVLGAsqTVGTYVMPSLIAQYHRSYPqISVQLLVqstrriaQKLVEGrlDLAIVGgeipfdlqrhLKVIALAEDEYVLV 177 Synechococcus s...
EES52622      92 HLRIGAsmTIGTYLLPPLLAQFLRANSrITASLAVanthqilHDLKASeiDLGLIEgeptrtqgisLDRKFLDHDRLVLI 171 Leptospirillum ...
NP_043632     96 NLIVGAsqTIGTYLMPRVLALFAQNHPqINIEVHVdstrkiaKRVLEGdiDIAVVGgnipeeieknLKVEDFVNDELILI 175 Odontella sinensis
YP_002755253  93 ALKLGSstTVAQFILPQLLARFRQFHPrVKISVISgntewavETLLKGsiMMGVIEgpvs---naeVYRRKILDDRMALM 169 Acidobacterium ...
YP_439809     92 NLKLAAsrTIGSYLLPPVLDVFCDRYPgVTIDVAEmntelveAALLTYerQLGFVEgayd---ttaFDSMVFGRDTLLAV 168 Burkholderia th...
Feature 1                                                              ######     ## ##          
P39647       169 APNQHPLagrkeis--isdlqneAWVTREKGSGTReyldhvls---snglrpksMFTISSnqgVKEAVINGmGLSVLSrs 243 Bacillus subtilis
YP_827874    169 TPPAHEFaaggeld--paalkaaSIILREHGSGTRqvveealkrarldtrglkiTLELDStesIKSAIAAGlGVGFVSrw 246 Solibacter usit...
NP_487993    177 LPVFHPFtkldtiq--kedlyklQFITLDSQSTIRkvidqvlsrseidtrrfkiEMELNSieaIKNAVQSGlGAAFVSts 254 Nostoc sp. PCC ...
NP_053815    177 LPKSHPFsklgdiq--kedlyrlRFIALDTQSTIRkvidkvlsqhgidssrfkiEMELNSieaIKNAVQSGlGAAFVSvs 254 Porphyra purpurea
NP_050683    177 LPKLHPFskleviq--kedlyrlRFITLDKQSTIRrvidtvlnehgidsarfkiEMELNSveaIKNAVQSGlGAAFVSis 254 Guillardia theta
YP_475803    178 GAPSCALdapalv-----dlltlPFITLDPQSSTRqtidrvlnrhginpgqlnvRLELSSieaIKNAVQAGlGVAFLSmv 252 Synechococcus s...
EES52622     172 DSLNHPLlpssgpvs-ldalrkvPFIGREPGSGTRqvierelleknlppdsfetVMTVDNpeaIKELVALGsGVAFISsl 250 Leptospirillum ...
NP_043632    176 IPKSHPFalkkkkkinkddlyhlNFITLNSNSTIRklidniliqiafepkqfniIMQLNSieaIKTAVSLGlGAAFVSss 255 Odontella sinensis
YP_002755253 170 ISGSHPWaqlkslp--lerlpeiPLLMRERGSGSRrvvesalrragmrmsrlniEMELDTtgaIIAGIEAGlGAGIVSlc 247 Acidobacterium ...
YP_439809    169 AASSHPLarkhrvg--vqdiadcDVLSREPGSGTRnavetafa---ehglefapRLSVGSaeaMNRLLLRGnAIAWVSrf 243 Burkholderia th...
Feature 1                                                      
P39647       244 vlrkdlihREISILhinnfslKRKLSYIHSPlmentknKEIFITML 289 Bacillus subtilis
YP_827874    247 algkeialGLLRTVpvkglriRRHFQFVYLQgpapgglEGEFLRFA 292 Solibacter usitatus Ellin6076
NP_487993    255 aiakelqmGVLHCTpidgvviKRTLWLIFNPnryrskaAEAFSQEI 300 Nostoc sp. PCC 7120
NP_053815    255 aiakelelGIVHWAqienvtiKRMLSIIVNPnrykskaTETFSQEI 300 Porphyra purpurea
NP_050683    255 aiakelelGLLHWVkiegvviKRTLSIITNPsryqakaSEIFSKEI 300 Guillardia theta
YP_475803    253 avgsdleqGRLKKLaveglqiRRTLWLAFNPeryqsqaATRFREGL 298 Synechococcus sp. JA-3-3Ab
EES52622     251 clr-phesGLLRTVavedfspVRNLWIFVPQkkt-sppADLFLQLL 294 Leptospirillum ferrodiazotrophum
NP_043632    256 aiekeielKTIEIVtiedikiTRILSIISNPecyrskaVDLFYNEL 301 Odontella sinensis
YP_002755253 248 avpkelrlGTIRALpvegldiVRPISLIRRAgpmpegpAGAFEQML 293 Acidobacterium capsulatum ATCC 51196
YP_439809    244 tieaeleaGALVPLrvtdmtvERDLTIIWHKgatlspsARIFRDLL 289 Burkholderia thailandensis E264

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