Conserved Protein Domain Family
C2B_Synaptotagmin-14_16

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cd08408: C2B_Synaptotagmin-14_16 
C2 domain second repeat present in Synaptotagmins 14 and 16
Synaptotagmin 14 and 16 are membrane-trafficking proteins in specific tissues outside the brain. Both of these contain C-terminal tandem C2 repeats, but only Synaptotagmin 14 has an N-terminal transmembrane domain and a putative fatty-acylation site. Previously all synaptotagmins were thought to be calcium sensors in the regulation of neurotransmitter release and hormone secretion, but it has been shown that not all of them bind calcium and this is indeed the case here. Of the 17 identified synaptotagmins only 8 bind calcium (1-3, 5-7, 9, 10). The function of the two C2 domains that bind calcium are: regulating the fusion step of synaptic vesicle exocytosis (C2A) and binding to phosphatidyl-inositol-3,4,5-triphosphate (PIP3) in the absence of calcium ions and to phosphatidylinositol bisphosphate (PIP2) in their presence (C2B). C2B also regulates also the recycling step of synaptic vesicles. C2 domains fold into an 8-standed beta-sandwich that can adopt 2 structural arrangements: Type I and Type II, distinguished by a circular permutation involving their N- and C-terminal beta strands. Many C2 domains are Ca2+-dependent membrane-targeting modules that bind a wide variety of substances including bind phospholipids, inositol polyphosphates, and intracellular proteins. Most C2 domain proteins are either signal transduction enzymes that contain a single C2 domain, such as protein kinase C, or membrane trafficking proteins which contain at least two C2 domains, such as synaptotagmin 1. However, there are a few exceptions to this including RIM isoforms and some splice variants of piccolo/aczonin and intersectin which only have a single C2 domain. C2 domains with a calcium binding region have negatively charged residues, primarily aspartates, that serve as ligands for calcium ions. This cd contains the second C2 repeat, C2B, and has a type-I topology.
Statistics
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PSSM-Id: 176053
View PSSM: cd08408
Aligned: 14 rows
Threshold Bit Score: 208.762
Threshold Setting Gi: 156403123
Created: 17-Sep-2009
Updated: 2-Oct-2020
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
XP_002213906 228 VPELLVGLAYNATtGRLSVEVIKGSHFRnmamnrAPDTYVKICLMAsngqELSRSKTSIRRGQPNPVYKETFIFQVAlfq 307 Florida lancelet
CAI12024     257 IPELLLGLLYNSTtGRLSAEVIKGSHFKnsasdkLPNTYVKLTMLDskgkEMSKCKTSVCRGQPNPTYKETFVFQVAlfq 336 zebrafish
XP_001177199 283 VPEILIGLAYNATtGRLDVEVIKGSHFKqraagrAPDSYAKVALMSssgfEISKSKTSVRRGQSSPTFKETFFFQVAqfq 362 purple urchin
Q17RD7       506 APELLVGLSYNATtGRLSVEMIKGSHFRnlavnrAPDTYGKLFLLNsvgqEMSRCKTSIRRGQPNPVYKETFVFQVAlfq 585 human
XP_001633287 170 APELLVSLCYQSLtGRLTVEVLKASNLRnvamqrAPDTYVKVSMFNsigqQLSKSKTSIRRSMYDPEFNETFVFQIIefe 249 starlet sea ane...
XP_001639938 133 RPEILISLEYRRLtGKLLLEVIKTRNLGmltdskAKEMYVSVKLIGsngeLISKGRTTPRRHMIDPEYNEMFLFHVPeqe 212 starlet sea ane...
XP_002129846 432 LPELLLSLSYNEMtGRLCVEVVKGSHFRnlamskPPDTFVKLVLLNsscqEMAQSKTTIRRSQPNPVFKEPFYFQVAlfq 511 Ciona intestinalis
NP_649453    446 VSELLLGLSYNGVtGRLSVEIIKGSQFRslslnkAPDTYVKMVMVSsigqEISRAKTSIRRGQPNPLFKETFAFQVAlfq 525 fruit fly
XP_002161287 295 MPELMVNLHYKDLtGRLYVEIIKGANFKtstmvvNPSVFIKIKCVAgdgkQIGKSKTIIHPPASDPEFNETFVYPMStid 374 green hydra
EEC10443     205 LPELLLGLAYNGTtGRLAAEVIKGSHFRnvamnrAPDTFVKLTLMSssgqEIARSKTSVRRGQPNPLFKETFVFQVAlfq 284 black-legged tick
XP_002213906 308 lsdvTLMFSVYNKRG-MKRKEMIGWFSLGLnnsgeeELNHWQDMRESk-gQQVCRWHVLL 365 Florida lancelet
CAI12024     337 lsevTLQVSVYSRRSsMKRRERVGWVALGLnstteeQEEHWTQMKESe-gQQVCHWHTLI 395 zebrafish
XP_001177199 363 lpemTLLFTVFNKKG-MKRKETIGWFSMGLsnsseeEIGHWNEMRESk-gHQVCRWHALL 420 purple urchin
Q17RD7       586 lsdvTLMISVYNRRT-MKRKEMIGWIALGQnssgeeEQDHWEEMKETk-gQQICRWHTLL 643 human
XP_001633287 250 lpsvSLMFSVVNIKK-MRRKEIMGWFSMGRdntgedEESHWKEMIEGk-gKAVKRWHVLS 307 starlet sea anemone
XP_001639938 213 lnsvTVLIALTSVSKtLGRKLKVGQLALGQnysseeEYRHWFEMTRSk-eKSIVRWHRIN 271 starlet sea anemone
XP_002129846 512 lvevSLMVSVYSSKT-IKKKEMLGWISLGQnssseaDEQHWLEMKEAk-gNTVSRWHTLI 569 Ciona intestinalis
NP_649453    526 lndvTLMISVYAKRH-MKKNEMVGWFSLGLnssgseEVAHWADVCEMpkgEMLARWHVLV 584 fruit fly
XP_002161287 375 lkeaTMMFTVLDARK-KKRGEIIGWFAFGKntsgtnESLHWNEMVKNp-dNDACRWQMLL 432 green hydra
EEC10443     285 lpdvTLMVSVYNKRS-MKRKEMIGWFSLGLnssgeeELSHWSDMRESk-gEQVCRWHVLL 342 black-legged tick
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