1DNK,2EI9,1I9Y,1I9Z,3G4T,2DDT,1DE9,1BIX,3NGO,1ATN,1DE8,1VYB,2F1N,2UYR,3FZI,3MPR,3NGN


Conserved Protein Domain Family
EEP
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cd08372: EEP 
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Exonuclease-Endonuclease-Phosphatase (EEP) domain superfamily
This large superfamily includes the catalytic domain (exonuclease/endonuclease/phosphatase or EEP domain) of a diverse set of proteins including the ExoIII family of apurinic/apyrimidinic (AP) endonucleases, inositol polyphosphate 5-phosphatases (INPP5), neutral sphingomyelinases (nSMases), deadenylases (such as the vertebrate circadian-clock regulated nocturnin), bacterial cytolethal distending toxin B (CdtB), deoxyribonuclease 1 (DNase1), the endonuclease domain of the non-LTR retrotransposon LINE-1, and related domains. These diverse enzymes share a common catalytic mechanism of cleaving phosphodiester bonds; their substrates range from nucleic acids to phospholipids and perhaps proteins.
Links
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Statistics
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PSSM-Id: 197306
Aligned: 20 rows
Threshold Bit Score: 127.598
Created: 19-Nov-2009
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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Conserved site includes 8 residues -Click on image for an interactive view with Cn3D
Feature 1:putative catalytic site [active site]
Evidence:
  • Comment:these residues are highly conserved in the EEP superfamily
  • Structure:1DE8: Human APE1 binds AP site of a 15-bp DNA substrate, contacts at 4A
  • Structure:3NGN: Human CNOT6L nuclease domain binds ribo-adenine-5'-monophosphate at the active site, contacts at 4A
  • Structure:1I9Z: Schizosaccharomyces pombe synaptojanin INPP5c domain binds I(1,4)P2 and Ca2+; contacts at 4A
Scroll to Sequence Alignment Display
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1         #                                            #                              
1DNK_A      3 IAAFNIRtfgetkm---snatlasYIVRIVrr----------ydIVLIQEvrdshlvavgkll----------------- 52  cattle
1I9Y_A     29 VASYNLNgc--------------sATTKLEnwlfpentpladiyVVGFQEivqltpqqvisadpakrrewescvkrllng 94  fission yeast
1I9Z_A     29 VASYNLNgc--------------sATTKLEnwlfpentpladiyVVGFQEivqltpqqvisadpakrrewescvkrllng 94  fission yeast
2DDT_A     12 VMTHNVYmlstnlypnwgqteradLIGAADyik--------nqdVVILNEvfdnsasdrl-------------------- 63  Bacillus cereus
1ATN_D      3 IAAFNIRtfgetkm---snatlasYIVRIVrr----------ydIVLIQEvrdshlvavgkll----------------- 52  cattle
1DE8_B     22 ICSWNVDglra---------wikkKGLDWVkee--------apdILCLQEtkcsenklpa-------------------- 64  human
1VYB_A     10 ILTLNINglnsa--------ikrhRLASWIksq--------dpsVCCIQEthltcrdth--------------------- 52  human
2F1N_A     18 VATWNLQgasa---------ttesKWNINVrqlisg---enavdILAVQEagsppstavdtgrvipspgi---pvreliw 82  Escherichia coli
2UYR_X     12 VMTHNVYmlstnlypnwgqteradLIGAADyik--------nqdVVILNEvfdasasdrl-------------------- 63  Bacillus cereus
NP_057698 116 LITWNIDgldl---------nnlsERARGVcsyla----lyspdVIFLQEvippyys----------------------- 159 human

Feature 1                                                                                     
1DNK_A     53 dylnqddpntyhYVVSEplgrn--------------sykeRYLFLFrpnk-----vsvlDTYQyddgcescgndsfsrEP 113 cattle
1I9Y_A     95 kctsgpgyvqlrSGQLV-----------------------GTALMIfckesclpsiknvEGTVkktglg---gvsgnkGA 148 fission yeast
1I9Z_A     95 kctsgpgyvqlrSGQLV-----------------------GTALMIfckesclpsiknvEGTVkktglg---gvsgnkGA 148 fission yeast
2DDT_A     64 --lgnlkkeypnQTAVLgrssgsewdktlgnyssstpedgGVAIVSkwp--------iaEKIQyvfakgcgpdnlsnkGF 133 Bacillus cereus
1ATN_D     53 dylnqddpntyhYVVSEplgrn--------------sykeRYLFLFrpnk-----vsvlDTYQyddgcgncgndsfsrEP 113 cattle
1DE8_B     65 --elqelpglshQYWSApsdk---------------egysGVGLLSrqc--------plKVSYgigdee----hdqegRV 115 human
1VYB_A     53 ---rlkikgwrkIYQANgkq-----------------kkaGVAILVsdktd----fkptKIKRdk-----------egHY 97  human
2F1N_A     83 nlstnsrpqqvyIYFSAvdal---------------ggrvNLALVSnrr--------adEVFVlspvr------qggrPL 133 Escherichia coli
2UYR_X     64 --lgnlkkeypnQTAVLgrssgsewdktlgnyssstpedgGVAIVSkwp--------iaEKIQyvfakgcgpdnlsnkGF 133 Bacillus cereus
NP_057698 160 -----ylkkrssNYEIItghe---------------egyfTAIMLKksrvkl-ksqeiiPFPStkm----------mrNL 208 human

Feature 1                         #                                              # #          
1DNK_A    114 AVVKFsshstkvkeFAIVALHSap-----------sdAVAEINSLYDVYldvqq--kwhlnDVMLMGDFNadcsyvtssq 180 cattle
1I9Y_A    149 VAIRFdye---dtgLCFITSHLaagyt------nydeRDHDYRTIASGLrfrrgrsifnhdYVVWFGDFNyrisltyeev 219 fission yeast
1I9Z_A    149 VAIRFdye---dtgLCFITSHLaagyt------nydeRDHDYRTIASGLrfrrgrsifnhdYVVWFGDFNyrisltyeev 219 fission yeast
2DDT_A    134 VYTKIkkn---drfVHVIGTHLqaedsmcgktspasvRTNQLKEIQDFIknkni---pnneYVLIGGDMNvnkinaennn 207 Bacillus cereus
1ATN_D    114 AVVKFsshstkvkeFAIVALHSap-----------sdAVAEINSLYDVYldvqq--kwhlnDVMLMGDFNadcsyvtssq 180 cattle
1DE8_B    116 IVAEFd-------sFVLVTAYVpnagrg---lvrleyRQRWDEAFRKFLkgla-----srkPLVLCGDLNvaheeidlrn 180 human
1VYB_A     98 IMVKGsiq---qeeLTILNIYApn-----------tgAPRFIKQVLSDLqrd------ldsHTLIMGDFNtplstldrst 157 human
2F1N_A    134 LGIRIg-------nDAFFTAHAiamr--------nndAPALVEEVYNFFrdsrdp-vhqalNWMILGDFNrepadlemn- 196 Escherichia coli
2UYR_X    134 VYTKIkkn---drfVHVIGTHLqaedsmcgktspasvRTNQLKEIQDFIknkni---pnneYVLIGGDMNvnkinaennn 207 Bacillus cereus
NP_057698 209 LCVHVnvs---gneLCLMTSHLestrg------haaeRMNQLKMVLKKMqeap-----esaTVIFAGDTNlrdrevtr-- 272 human

Feature 1                                                                          #          
1DNK_A    181 w-------------------ssirlrtsstFQWLIpd------saDTTAts-------------tNCAYDRIVVagsllq 222 cattle
1I9Y_A    220 vpciaqgklsylfeydqlnkqmltgkvfpfFSELPit------fpPTYKfdigtdiydtsdkhrvPAWTDRILYrgel-- 291 fission yeast
1I9Z_A    220 vpciaqgklsylfeydqlnkqmltgkvfpfFSELPit------fpPTYKfdigtdiydtsdkhrvPAWTDRILYrgel-- 291 fission yeast
2DDT_A    208 d------------------seyasmfktlnASVPSytg-----htATWDattnsiak-ynfpdspAEYLDYIIAskdha- 262 Bacillus cereus
1ATN_D    181 w-------------------ssirlrtsstFQWLIpd------saDTTAts-------------tNCAYDRIVVagsllq 222 cattle
1DE8_B    181 pkgnkkna----gftpqerqgfgellqavpLADSFrhlypntpyaYTFWtymmna-----rsknvGWRLDYFLLshsll- 250 human
1VYB_A    158 rqkvn-----------kdtqelnsalhqadLIDIYrtlhp-ksteYTFFsap----------hhtYSKIDHIVGskall- 214 human
2F1N_A    197 ----------------------ltvpvrraSEIISpa-------aATQTs---------------QRTLDYAVAgnsvaf 232 Escherichia coli
2UYR_X    208 d------------------seyasmfktlnASVPSytg-----htATWDattnsiak-ynfpdspAEYLDYIIAskdha- 262 Bacillus cereus
NP_057698 273 -----------------------cgglpnnIVDVWeflgkpkhcqYTWDtqmnsnl---gitaacKLRFDRIFFraaaee 326 human

Feature 1                                      ##       
1DNK_A    223 ssvvpgSAAPFDfqaaygls-----nemalaiSDHYPVEVTL 259 cattle
1I9Y_A    292 ---vphSYQSVPly-----------------ySDHRPIYATY 313 fission yeast
1I9Z_A    292 ---vphSYQSVPly-----------------ySDHRPIYATY 313 fission yeast
2DDT_A    263 -npsyiENKVLQpkspqwtvtswfqkytyndySDHYPVEATI 303 Bacillus cereus
1ATN_D    223 ssvvpgSAAPFDfqaaygls-----nemalaiSDHYPVEVTL 259 cattle
1DE8_B    251 --palcDSKIRSka----------------lgSDHCPITLYL 274 human
1VYB_A    215 --skckRTEIITny-----------------lSDHSAIKLEL 237 human
2F1N_A    233 -rpsplQAGIVYgarr------------tqisSDHFPVGVSR 261 Escherichia coli
2UYR_X    263 -npsyiENKVLQpkspqwtvtswfqkytyndySDHYPVEATI 303 Bacillus cereus
NP_057698 327 ghiiprSLDLLGlekld----------cgrfpSDHWGLLCNL 358 human

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