Conserved Protein Domain Family
alcohol_DH_plants

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cd08301: alcohol_DH_plants 
Plant alcohol dehydrogenase
NAD(P)(H)-dependent oxidoreductases are the major enzymes in the interconversion of alcohols and aldehydes or ketones. Alcohol dehydrogenase in the liver converts ethanol and NAD+ to acetaldehyde and NADH, while in yeast and some other microorganisms ADH catalyzes the conversion acetaldehyde to ethanol in alcoholic fermentation. There are 7 vertebrate ADH 7 classes, 6 of which have been identified in humans. Class III, glutathione-dependent formaldehyde dehydrogenase, has been identified as the primordial form and exists in diverse species, including plants, micro-organisms, vertebrates, and invertebrates. Class I, typified by liver dehydrogenase, is an evolving form. Gene duplication and functional specialization of ADH into ADH classes and subclasses created numerous forms in vertebrates. For example, the A, B and C (formerly alpha, beta, gamma) human class I subunits have high overall structural similarity, but differ in the substrate binding pocket and therefore in substrate specificity. In human ADH catalysis, the zinc ion helps coordinate the alcohol, followed by deprotonation of a histidine (His-51), the ribose of NAD, a serine (Ser-48) , then the alcohol, which allows the transfer of a hydride to NAD+, creating NADH and a zinc-bound aldehyde or ketone. In yeast and some bacteria, the active site zinc binds an aldehyde, polarizing it, and leading to the reverse reaction. ADH is a member of the medium chain alcohol dehydrogenase family (MDR), which has a NAD(P)(H)-binding domain in a Rossmann fold of an beta-alpha form. The NAD(H)-binding region is comprised of 2 structurally similar halves, each of which contacts a mononucleotide. A GxGxxG motif after the first mononucleotide contact half allows the close contact of the coenzyme with the ADH backbone. The N-terminal catalytic domain has a distant homology to GroES. These proteins typically form dimers (typically higher plants, mammals) or tetramers (yeast, bacteria), and have 2 tightly bound zinc atoms per subunit, a catalytic zinc at the active site and a structural zinc in a lobe of the catalytic domain. NAD(H) binding occurs in the cleft between the catalytic and coenzyme-binding domains at the active site, and coenzyme binding induces a conformational closing of this cleft. Coenzyme binding typically precedes and contributes to substrate binding.
Statistics
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PSSM-Id: 176261
View PSSM: cd08301
Aligned: 30 rows
Threshold Bit Score: 627.015
Threshold Setting Gi: 168010618
Created: 24-Dec-2009
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
Feature 1:NAD binding site [chemical binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                             ##                                      
P14674      9 IRCKAAVAWEagKPLVMEEVDVAPPQKmEVRLKILYTSLCHTDVYFWEAkgq-npVFPRILGHEAAGIVESVGEGVTeLA 87  potato
Q9FH04     17 IRCKAAVSRKagEPLVMEEIMVAPPQPfEVRIRIICTALCHSDVTFWKLqvp-paCFPRILGHEAIGVVESVGENVKeVV 95  thale cress
ABK25905   40 ITCRAAIAWGpkQPLVIEEVQVDPPQAmEVRIKITHTSLCQSDVTFSIHgdeslqAFPCIFGHEGAGIVESVGEGVTdIK 119 Sitka spruce
Q9SK86     14 ITCKAAICRKagEALVIEDIHVDPPQAyEVRIKILCTSLCHTDLTFWKLsfgpisRFPRILGHEAVGVVESIGENVDgFK 93  thale cress
ABR17509   10 ITCKAAVAWGpgEPMRIEEVQVAPPQPmEVRIKVVATSICRSDLTLWESrgh-ipIFPRIFGHEATGIVESVGEGVTdLR 88  Sitka spruce
ABK24931   10 ITCKAGVVWEagQPISIQQIQVAPPQSkEVRIKITHTSLCQTDIYFWKGkdg-gaWFPRILGHEGAGIVESVGSEVTeLK 88  Sitka spruce
A1L4Y2      9 ITCKAAVVWGpkVPLVIQEICVDPPQKmEVRVKILYSSICHTDLGCWNGtneaerAFPRILGHEAVGIVESVGEGVKdVK 88  thale cress
Q8VZ49      8 ITCKAAVAWGagEPLVMEDVKVDPPQRlEVRIRILFTSICHTDLSAWKGeneaqrAYPRILGHEAAGIVESVGEGVEeMM 87  thale cress
Q9SK87     12 IRCKAAILRKagEPLVIEEIQVDPPQAyEVRIKILCTSLCHTDVTFWKLdsgplaRFPRILGHEAVGVVESIGEKVDgFK 91  thale cress
Q0V7W6     15 IRCKAAVSRKpgEALVIEEIHVDPPQAyEVRIKIICTSLCHTDVSFSKIdsgplaRFPRILGHEAVGVIESIGEHVNgFQ 94  thale cress
Feature 1                                                                                     
P14674     88 PGDHVLPVFTGECKDCAHCKSEESNMCSLLrintdrGVMINDGQSRFSIn---------GKPIYHFv-GTSTFSEYTVVH 157 potato
Q9FH04     96 EGDTVLPTFMPDCGDCVDCKSHKSNLCSKFpfkvspWMPRYDNSSRFTDl--------nGETLFHFl-NVSSFSEYTVLD 166 thale cress
ABK25905  120 EGDHVIPLFIGECGDCACCKSNKTNLCEKF------NIVRNDVKEPRFSi--------rGKAVHHFm-NTSTFSEFTVVD 184 Sitka spruce
Q9SK86     94 QGDVVLPVFHPYCEECKDCKSSKTNWCDRYaedfisNTRRYGMASRFKDs---------SGEVIHHflFVSSFSEYTVVD 164 thale cress
ABR17509   89 EGDHVLTVFQGECKKCRHCKSDKSNACETLgie-rnGLMHSDKKTRFSIn---------GKPIYHFv-AVSSFSEYTVVH 157 Sitka spruce
ABK24931   89 AGDHVIPSYMAECKECELCDRGKNNMCEVFifnylsGVAYTDKKCRFSLn---------GKPIHHFf-GLSTFSEYTVVH 158 Sitka spruce
A1L4Y2     89 EGDYVIPTFNGECGECKVCKREESNLCERYhvdpmkRVMVNDGGTRFSTtinkdggssqSQPIYHFl-NTSTFTEYTVLD 167 thale cress
Q8VZ49     88 AGDHVLPIFTGECGDCRVCKRDGANLCERFrvdpmkKVMVTDGKTRFFTsk-------dNKPIYHFl-NTSTFSEYTVID 159 thale cress
Q9SK87     92 QGDVVLPVFHPQCEECKECISPKSNWCTKYtndylsNTRRYGMTSRFKDs--------rGEDIHHFi-FVSSFTEYTVVD 162 thale cress
Q0V7W6     95 QGDVVLPVFHPHCEECRDCKSSKSNWCARFaddflsNTRRYGMTSRFKDs--------fGEDIYHFl-FVSSFSEYTVVD 165 thale cress
Feature 1                         #   #                    #####                   ##   #     
P14674    158 VGCVAKINPlAPLDKVCVLSCGISTGLGATLNVAKPtKGSSVAIFGLGAVGLAAAEGARIAGAsRIIGVDLNASRFEQAK 237 potato
Q9FH04    167 VANVVKIDSsIPPSRACLLSCGVSTGVGAAWETAKVeKGSTVVIFGLGSIGLAVAEGARLCGAsRIIGVDINPTKFQVGQ 246 thale cress
ABK25905  185 SKCVAKINPeTPLDKACLFGCGITTGVGAALYTADIePGSTVAIFGLGTVGLAVAEGARIRGAsKIIGVDTNPNKFIKAK 264 Sitka spruce
Q9SK86    165 IAHLVKISPeIPVDKAALLSCGVSTGIGAAWKVANVeEGSTIAIFGLGAVGLAVAEGARLRGAaKIIGIDTNSDKFELGK 244 thale cress
ABR17509  158 SECIVKLSPdVPLGKISVLGCGVSTGFGAAWKIANVgKGSTVVIFGLGAVGLAVAQGAKLRGAsRIIGVDINPDKFERGK 237 Sitka spruce
ABK24931  159 MSCVAKVNReAPLDKICLLGCGVPAGLGAAWNGGKVkPGDSVAIFGLGTIGLAVAEGARIAGAsRVLGIDINPAKEETSK 238 Sitka spruce
A1L4Y2    168 SACVVKIDPnSPLKQMSLLSCGVSTGVGAAWNIANVkEGKSTAVFGLGSVGLAVAEGARARGAsRIIGVDANASKFEKGK 247 thale cress
Q8VZ49    160 SACVLKVDPlFPLEKISLLSCGVSTGVGAAWNVADIqPASTVAIFGLGAVGLAVAEGARARGAsKIIGIDINPDKFQLGR 239 thale cress
Q9SK87    163 IAHLVKISPeIPVDIAALLSCSVATGLGAAWKVADVeEGSTVVIFGLGAVGLAVAEGVRLRGAaKIIGVDLNPAKFEIGK 242 thale cress
Q0V7W6    166 IAHLVKISPdIPVDKAALLSCGVSTGIGAAWKVANVeKGSTVAVFGLGAVGLAVGEGARLRGAgKIIGVDLNPEKFELGK 245 thale cress
Feature 1                                        ##   ##                 ##                   
P14674    238 KFGVTEFVNPKDYSK-PVQEVIAEMTdGGVDRSVECTGHiDAMISAFECVhDGWGVAVLVGVphkeaVFKTHPMNFLn-E 315 potato
Q9FH04    247 KFGVTEFVNSMTCEKnRVSEVINEMTdGGADYCFECVGSsSLVQEAYACCrQGWGKTITLGVdkpgsQICLDSFDVLhhG 326 thale cress
ABK25905  265 ALGVTECINPKDHEK-PIQEVITEKSeGGVDYSFECIGNtDVLYQAFLATrPALGLTVLLGVdisprKISLHPIDLFs-G 342 Sitka spruce
Q9SK86    245 KFGFTDFINPTLCGEkKISEVIKEMTeGGVDYSFECVGLaSLLNEAFISTrTGTGKTVMLGMekhaaPISLGSFDLLr-G 323 thale cress
ABR17509  238 AFGVTEFINPNDYKQ-PTQEVVKEITtGGADYCFECVGNvELMRAALESCcDGWGMAVLIGVpsgkmELSAHYGPLLn-G 315 Sitka spruce
ABK24931  239 RFGVTEFLNPSDFDK-PIQEVIREMTkGGVNSSYECVGRvELMLAALQCShPAWGRTIILGLdemdkKLSFNPLEILq-G 316 Sitka spruce
A1L4Y2    248 LMGVTDFINPKDLTK-PVHQMIREITgGGVDYSFECTGNvDVLREAFLSThVGWGSTVLVGIyptprTLPLHPMELFd-G 325 thale cress
Q8VZ49    240 EAGISEFINPKESDK-AVHERVMEITeGGVEYSFECAGSiEALREAFLSTnSGVGVTVMLGVhaspqLLPIHPMELFq-G 317 thale cress
Q9SK87    243 RFGITDFVNPALCGEkTISEVIREMTdVGADYSFECIGLaSLMEEAFKSTrPGSGKTIVLGMeqkalPISLGSYDLLr-G 321 thale cress
Q0V7W6    246 KFGFTDFINSTLCGEnKISEVIKEMTgGGVDYSFECVGLpSLLTEAFSSTrTGSGKTVVLGIdkhltPVSLGSFDLLr-G 324 thale cress
Feature 1          ###                                                 #    
P14674    316 RTLKGTFFGNYKPrsDIPSVVEKYMNKELELEKFITHTLPFAEINKAFDLMLKGEGLRCIIT 377 potato
Q9FH04    327 KILMGSLFGGLKAktHIPILLKRYLSNELELDKFVTHEMKFEEINDAFQLLLEGKCIRCVLW 388 thale cress
ABK25905  343 RRIVATTFGGVKGktQLPYLVEMFMNKEIRVEEFITHELPFSEINRAFELLLEGSCLRCVLH 404 Sitka spruce
Q9SK86    324 RVICGSLFGGLKSklDIPILVDHYLKKELNLDSFITHELNFKEINKAFALLEEGKSLRCILW 385 thale cress
ABR17509  316 RTLKGTLLGGWKTrsELPMLVEMYMKKEIQIDEYVTHEFPLADINKAYQLMKEGKCLRCVLH 377 Sitka spruce
ABK24931  317 RKLKYVAFGGFKNs-DIPKLVDKYMNNEFDLDKYITQRMAFSDINAAMEMLVAGKCLRCVLQ 377 Sitka spruce
A1L4Y2    326 RRITGSVFGGFKPksQLPNFAQQCMKGVVKLEPFITNELPFEKINDAFQLLRDGKSLRCILQ 387 thale cress
Q8VZ49    318 RSITASVFGGFKPktQLPFFITQCLQGLLNLDLFISHQLPFHDINEAMQLLHQGKALRCLLH 379 thale cress
Q9SK87    322 RTVCGTLFGGLKPklDIPILVDRYLKKELNLEDLITHELSFEEINKAFHLLAEGNSIRCIIW 383 thale cress
Q0V7W6    325 RHVCGSLFGGLKPklDIPILVDHYLKKELNLDSFITHELKFEEINKAFDLLVQGKSLRCILW 386 thale cress

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