Conserved Protein Domain Family
CAD2

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cd08298: CAD2 
Cinnamyl alcohol dehydrogenases (CAD)
These alcohol dehydrogenases are related to the cinnamyl alcohol dehydrogenases (CAD), members of the medium chain dehydrogenase/reductase family. NAD(P)(H)-dependent oxidoreductases are the major enzymes in the interconversion of alcohols and aldehydes, or ketones. Cinnamyl alcohol dehydrogenases (CAD) reduce cinnamaldehydes to cinnamyl alcohols in the last step of monolignal metabolism in plant cells walls. CAD binds 2 zinc ions and is NADPH- dependent. CAD family members are also found in non-plant species, e.g. in yeast where they have an aldehyde reductase activity. The medium chain dehydrogenases/reductase (MDR)/zinc-dependent alcohol dehydrogenase-like family, which contains the zinc-dependent alcohol dehydrogenase (ADH-Zn) and related proteins, is a diverse group of proteins related to the first identified member, class I mammalian ADH. MDRs display a broad range of activities and are distinguished from the smaller short chain dehydrogenases (~ 250 amino acids vs. the ~ 350 amino acids of the MDR). The MDR proteins have 2 domains: a C-terminal NAD(P) binding-Rossmann fold domain of a beta-alpha form and an N-terminal catalytic domain with distant homology to GroES. The MDR group contains a host of activities, including the founding alcohol dehydrogenase (ADH), quinone reductase, sorbitol dehydrogenase, formaldehyde dehydrogenase, butanediol DH, ketose reductase, cinnamyl reductase, and numerous others. The zinc-dependent alcohol dehydrogenases (ADHs) catalyze the NAD(P)(H)-dependent interconversion of alcohols to aldehydes or ketones. Active site zinc has a catalytic role, while structural zinc aids in stability. ADH-like proteins typically form dimers (typically higher plants, mammals) or tetramers (yeast, bacteria), and generally have 2 tightly bound zinc atoms per subunit. The active site zinc is coordinated by a histidine, two cysteines, and a water molecule. The second zinc seems to play a structural role, affects subunit interactions, and is typically coordinated by 4 cysteines.
Statistics
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PSSM-Id: 176258
View PSSM: cd08298
Aligned: 35 rows
Threshold Bit Score: 428.91
Threshold Setting Gi: 119961812
Created: 5-Feb-2009
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
Feature 1:putative NAD(P) binding site [chemical binding site]
Evidence:
  • Comment:MDR family binds NAD(P) as a cofactor
  • Comment:defined by comparison to MDR alcohol dehydrogenase like family

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                  ###  #                                
YP_642849      1 MRAMVLRApg----gPLRPEELPVPEPGs-GEVLVRVRACGVCRTDLHVVDGELRe----pKLPLVVGHQIVGVVERVGE 71  Rubrobacter xyl...
YP_946159     60 MYAAVLRApgpienhPLVYGEVPEPEPGp-GEVSISVLACGVCRSNLHMVEGDWLpgt-paALPIIPGHEVVGRVKAVGP 137 Arthrobacter au...
YP_875447      4 MRAMVLNKtgpieenPLELAEIERHRVNgpREVLLKIQACGVCHSQLHSIEGDWKhlgippALPTVPGHEVVGEVVETGP 83  Cenarchaeum sym...
YP_024027      1 MKAMVLEKvgkveenPLKYKDVDIKKPEg-FEILIKNLACGVCHSNLHMIEGDWVqyglpgKSNIIPGHEIIGRVEELGD 79  Picrophilus tor...
ABZ08489       5 MRAMVLSKlgpvesnPLKLTEIDRHEISnpKEVLVKIEACGVCHSQLHSIEGDWKdlgippTLPTVPGHEVVGKIVEVGS 84  uncultured mari...
ABX56663       1 MKALLLTTpsaiekqPLRLEQVDIPYPKe-DEVLVKIEACGVCRSNLHMIEGEWLqrgipaKLPIIPGHEIVGRVEDVGD 79  Methylokorus in...
YP_924511      1 MHANQLTTpahlshhPLLWTDVDRPEPSr-GQLLVKVAGCGVCRSNLHMIEGDWVeggvpaISPIIPGHEVTGRVAAIGA 79  Nocardioides sp...
EET89782       1 MKGMVLHKyakvedrPLSMENFEAGGIKs-DEVLVKVIACGVCRTDLHIVEGQLGn----aNLPLVPGHEIVGKVEEVGK 75  uncultured eury...
YP_003239153   1 MRAMLLHKprpieeqPLVLTELPEPEPGp-GQVRLRVEVCGVCHTDLHTVEGDLPl----pKLPLIPGHQVVGIVDAVGE 75  Ammonifex degen...
EEZ92945       3 TKAAFIEKpekieenPLKIKEFELPELRe-NEVLIKVNACGVCRTDLHVVEGELGe-----IKNIVPGHEVVGTIEKVGK 76  Candidatus Parv...
Feature 1                                                                                   #   #
YP_642849     72 GVERfsPGDRVGVPWLGrTCGRCRYCRTGrENLCGearFTGYqlDGGFAEYARADARFCLPVPEgyPDLQAAPLLCAGLI 151 Rubrobacter xyl...
YP_946159    138 GVDNfaVGDRIGVQPIWsTCGRCGHCIGGyEQRCRqrqITGEtrDGGYAEVMLANAAFACKIPDslSDTEAAPLFCPGIT 217 Arthrobacter au...
YP_875447     84 GVTKfsVGDHAGVSPLLeSCMDCAYCTGGrEYLCDsstILGEsmRGGYAEYLNVSEDFATLVPAdmNPEHAAPLFCAGIT 163 Cenarchaeum sym...
YP_024027     80 NVTDlkKGDIVGLQPLYsACGKCEYCLSGrEHLCPygkWTGEtvDGGYAEYMIADSRYVNKVPSn-IDPVNAPLFCPGVT 158 Picrophilus tor...
ABZ08489      85 NVTKfrVGERVGITPLLgSCLDCQYCNEGkEYLCEkmdVTGEslKGGYTEYITVSEDFATKVPDtmKPEYAAPLFCAGIT 164 uncultured mari...
ABX56663      80 RVRHfkKGDRVGLQPLWlSCGHCSYCWTGrEELCPekeITGEtvDGGYAEYVLGKEDHLYPVPEtlSAMEAAPLFCPGIT 159 Methylokorus in...
YP_924511     80 GVEGfeVGDPVGVQPLWwTCGECEFCTTGrEQLCHeriITGEhvDGGYAEYMLSFAEHTYRIPEslDLIDAAPLFCPGIT 159 Nocardioides sp...
EET89782      76 EVWNlsKGDLVGVPWLHsTCGKCEYCLSGrENLCHskeFTGYtvNGGYAEYVKARSTFAFRLENdaDPYKTAPLLCAGII 155 uncultured eury...
YP_003239153  76 GVARvkVGDRVGVPWLYhTCGSCRFCRNGqENLCEkgrFTGYhvDGGYAEFMVAEEDFVCPLPPelEPVKAAPLLCAGII 155 Ammonifex degen...
EEZ92945      77 EVSAqkIGKKVGVAWLYsSCGSCEYCLSGrENLCQnkqFTGFsrNGGYSEYIIADNRFIFDLPKglKDEEIAPLLCSGAI 156 Candidatus Parv...
Feature 1                           ######                 ##     #          #                   
YP_642849    152 GYRALrmag--daerLGLYGFGASAHIILQVARHQGRRVFAFTRDgdeEAQRFARRLGAEWAGGSgqr----------pp 219 Rubrobacter xyl...
YP_946159    218 AYGAVkkaqprpgqnVAVFGVGGVGHLALQMAGLTGAHVNAVSRSa--SARALAEQLGAHGAYVPagsdgg----relrd 291 Arthrobacter au...
YP_875447    164 AYKAVkaagpapgsrVGIFGIGGVGHMAVQFARLAGAEVTAVSRSa--EHLQVAAELGASSTAVFgse---------lpg 232 Cenarchaeum sym...
YP_024027    159 AYTAVkkaelspdktVYIVGIGGVGHIALQIAKLYGSRVVAVTTSk--NHEDLAYKMGADDVVIMdrnyen----admla 232 Picrophilus tor...
ABZ08489     165 AYKAVkaaepeknkkIAIFGIGGVGHMAIQFAKIEGCEVSGVSRKe--KHLEVAKKLGADNVFAYssnqkqflqdvndky 242 uncultured mari...
ABX56663     160 AYHALkrvgnvmgkrVAIFGIGGVGHMALQFARVAGATTIAISRNp--IHLQVAKELGAEECIHGedrsal---eklkre 234 Methylokorus in...
YP_924511    160 AYGALdkidvgpgdvVAVFGLGGVGHMAVQMAVLTGAEVVAVGRTp--AHLEVAKELGAARTVDStdqda-----ldlia 232 Nocardioides sp...
EET89782     156 GYRAFklaapppggsIGMFGFGSSAHITLQLASKLGFEAVAVSRNr--EHIDLAKRLGAAATYSYdnle--------sir 225 uncultured eury...
YP_003239153 156 GYRALklsgikpggkLGLYGFGASAHLAIQVATYWGCEVFVFSRGa--HHRELARELGAVWTGTPqes----------pp 223 Ammonifex degen...
EEZ92945     157 GYRSFkltnsspgstIAMFGFGGSAHLTLQLAKKLGHKVIIISRNe--NHLKLASELGADFTFSPkngdvy----enlkd 230 Candidatus Parv...
Feature 1                  ## #                 ##                         ###                   
YP_642849    220 EELDAAIIFAPVGALVPLALRAVArGGTVVCAGIHmsdIPSFPYel-lWGERQLRSVANLTRRDGEEFLALAPEVpVRTE 298 Rubrobacter xyl...
YP_946159    292 GSQDAAIVFAPSDLAVAEALRVIKpGGVVVLGVAQs--LGVMDI----GDEKTVVGTVLGTRQDMADVLTLAVDGkLHAV 365 Arthrobacter au...
YP_875447    233 GPLDTAIVFAPSDAATTAAIRAVKkGGTIVVGTIGe--IRGFVA----FEEKTIRGTLIGSPRDMEDVIRIAHENsLQVV 306 Cenarchaeum sym...
YP_024027    233 KTADAAIIFAPADAAIKNSIKFVK-DGGVVVQGVRg-nQGEFDF----TRELKIKGSKIGTRQDMNEVLKLAADGkIKVE 306 Picrophilus tor...
ABZ08489     243 GLFDAAIVFAPVDQVTDTAIKSIKkGGTVVIATVGk--IPNFLA----FEEKTVRGTLIGSRKDMEDVIKISDEHkLEVV 316 uncultured mari...
ABX56663     235 GAVDCAIVFAPSSAVAQLAISLVNaGGKVVLGVNVe--LGSFSF----VEEKTVLGTVIGSREEMREVLRLAEERkVKPI 308 Methylokorus in...
YP_924511    233 DTADAVLTFAGSDEVTAQAMRTLKwGGTLVNGVPIn--VEEFHF----NKGQTIKGSILGNREQMRAVLQLAAEGkIRTV 306 Nocardioides sp...
EET89782     226 GKLDSAIVFAPASRPILDALESVKsGGRVIVADIYvgnMEPFSYerylFNERELKSVEANTREDAVEFLALSRRLkISSV 305 uncultured eury...
YP_003239153 224 EKLDSSIIFAPAGWLVPLALEHLDkGGVLVLAGITmtdIPQLKYel-lYHERVVCSVANATRQDAREFLALAAVIpVKTT 302 Ammonifex degen...
EEZ92945     231 KDIEDAIIFAPSATPIMNALKVIKpGGRVVVAGIHidgEIKIDYdsqlFHEKTLLSVESYTREDMLEYLKLATNLgIKPV 310 Candidatus Parv...
Feature 1                                   
YP_642849    299 VRPYPLegANQALDDLRSGRLTGAAVL 325 Rubrobacter xylanophilus DSM 9941
YP_946159    366 HEDFPLseANLVLQRLKKGQLHGRAVL 392 Arthrobacter aurescens TC1
YP_875447    307 TERFPLegANEALQKLKNSKIKARAVL 333 Cenarchaeum symbiosum A
YP_024027    307 SKTYKLeeANEALKALKHGEVNGRAVL 333 Picrophilus torridus DSM 9790
ABZ08489     317 TESFPLeqANEVLVRLKNSEIDGRAVL 343 uncultured marine crenarchaeote HF4000_APKG3D24
ABX56663     309 SQGFSLedAPEALMKLKKGEIIMRAVL 335 Methylokorus infernorum V4
YP_924511    307 VERFPMaqAEDALTRLANGELRSRGVL 333 Nocardioides sp. JS614
EET89782     306 VETVGLekANEALEKLKKGNVNGAIVL 332 uncultured euryarchaeote ARMAN-2
YP_003239153 303 VETFPLeeANEALQKLKAGKINGAGVL 329 Ammonifex degensii KC4
EEZ92945     311 YADIKLedVNSALTELKNSEVMGVKVI 337 Candidatus Parvarchaeum acidiphilum ARMAN-4

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