1LLU,1LLU,1RJW,2HCY,3MEQ


Conserved Protein Domain Family
CAD3

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cd08297: CAD3 
Click on image for an interactive view with Cn3D
Cinnamyl alcohol dehydrogenases (CAD)
These alcohol dehydrogenases are related to the cinnamyl alcohol dehydrogenases (CAD), members of the medium chain dehydrogenase/reductase family. NAD(P)(H)-dependent oxidoreductases are the major enzymes in the interconversion of alcohols and aldehydes, or ketones. Cinnamyl alcohol dehydrogenases (CAD) reduce cinnamaldehydes to cinnamyl alcohols in the last step of monolignal metabolism in plant cells walls. CAD binds 2 zinc ions and is NADPH- dependent. CAD family members are also found in non-plant species, e.g. in yeast where they have an aldehyde reductase activity. The medium chain dehydrogenases/reductase (MDR)/zinc-dependent alcohol dehydrogenase-like family, which contains the zinc-dependent alcohol dehydrogenase (ADH-Zn) and related proteins, is a diverse group of proteins related to the first identified member, class I mammalian ADH. MDRs display a broad range of activities and are distinguished from the smaller short chain dehydrogenases (~ 250 amino acids vs. the ~ 350 amino acids of the MDR). The MDR proteins have 2 domains: a C-terminal NAD(P) binding-Rossmann fold domain of a beta-alpha form and an N-terminal catalytic domain with distant homology to GroES. The MDR group contains a host of activities, including the founding alcohol dehydrogenase (ADH), quinone reductase, sorbitol dehydrogenase, formaldehyde dehydrogenase, butanediol DH, ketose reductase, cinnamyl reductase, and numerous others. The zinc-dependent alcohol dehydrogenases (ADHs) catalyze the NAD(P)(H)-dependent interconversion of alcohols to aldehydes or ketones. Active site zinc has a catalytic role, while structural zinc aids in stability. ADH-like proteins typically form dimers (typically higher plants, mammals) or tetramers (yeast, bacteria), and generally have 2 tightly bound zinc atoms per subunit. The active site zinc is coordinated by a histidine, two cysteines, and a water molecule. The second zinc seems to play a structural role, affects subunit interactions, and is typically coordinated by 4 cysteines.
Statistics
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PSSM-Id: 176257
View PSSM: cd08297
Aligned: 71 rows
Threshold Bit Score: 333.732
Threshold Setting Gi: 39960477
Created: 5-Feb-2009
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 25 residues -Click on image for an interactive view with Cn3D
Feature 1:NAD binding site [chemical binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                  ###  #                                
1LLU_H         7 MKAAVVHaygaplrIEEVKVPLPGPG-----QVLVKIEASGVCHTDLHAAEGDWpvk--pplPFIPGHEGVGYVAAVGsg 79  Pseudomonas aer...
EAL20142      10 MRALVVPepg-vnyYTLQEQDTPSPKg---tKVLLHIKASGLCHTDSIVRDGSFgg----nwPIIGGHEPAGEVVAVGdn 81  Cryptococcus ne...
XP_760628     10 MRAATIKdfk--kgYEIKDIDVPTDLgp--nDILIKIAAAGYCHTDLQVMEGVYesqg-akpGLVGSHEPAGTVVKTGsd 84  Ustilago maydis...
XP_772242      1 MKAAQIVgfe--apYKVTDIPKPVPQg---nQILLRVKAAGFCHTDEAVKMGYTgs----plPLTGGHEGAGLVEATGgd 71  Cryptococcus ne...
XP_001796102  29 MKAQFLEeyk--tpYKLHSVPIPVPStp--hDILIKVDAASYCHTDFVLAEGQMpglp-ksfPHIGCHEFAGTVVAHYvs 103 Phaeosphaeria n...
XP_001903156  10 MKAQILPsfn--apYRLTTLPLPSFTadsphEMLVRVLAASYCHTDLVMAQGQMppfp-psfPHIGCHEFSGVVVSLPeh 86  Podospora anser...
EED24059      66 MKAQQLDayn-tpyVFRDSVPVPTPThp--yDLLIRVDAASYCHTDYVLASGHMasveqpvfPHAGCHEFSGTVVALPpn 142 Talaromyces sti...
EED51852       9 MWAAQITefnkpytISTVNVPPLRPN-----ELLVKIHAAGFCHSDIQVLRGELns----plPLIPSHEPVGTVVQVGke 79  Aspergillus fla...
EED55901       6 MKAIQIQqyn--epYHISDVPIPKPKp---hQVLVRIQAAGFCHTDLMALNNEFnt----klPFIGSHEPAGIIEEVGse 76  Aspergillus fla...
EEU37448       7 QTAAVVErfg--epYVLKQIPRPENPkg--pDLLIKVLAASYCHTDAVFAAGLLsq----dlPRIGCHEFAGEVVAVGpe 78  Nectria haemato...
Feature 1                                                                                        
1LLU_H        80 v----trvKEGDRVGIPWly-taCGCCEHClt-------------gwETLCESQQNtgy-svNGGYAEYVLADPNYVGIL 140 Pseudomonas aer...
EAL20142      82 a----eevSVGDRVVALLpk-dpCDTCPDCtm-------------gdWKYCKFMKFggi-naDGFFSEYALVEAKFCVHI 142 Cryptococcus ne...
XP_760628     85 ag--ksgiKVGDRVGSINty-gyCGKCDSCkq--------------gKQLCDNMPGmlgltlNGGFSQYMKADARVVSKI 147 Ustilago maydis...
XP_772242     72 v----tnfTVGDRVAALLfr-epCGQCGECkn-------------nsAVFCDNIKMngf-nvDGAFAEYMIVDQRFTLHV 132 Cryptococcus ne...
XP_001796102 104 psqqakefYIGSRVGVPGrafhaCGKCFECkhdrpkdp-vaedddgySVHCIDAENngl-srDGGFAEYALVDARQLAPI 181 Phaeosphaeria n...
XP_001903156  87 ss---sqyRIGDRVGVPGrsfrpCGKCFECsrepdndkniyvdgggySVYCASAGNhgi-stPGGFREFAIVDSRQVTSL 162 Podospora anser...
EED24059     143 sa--qsdfKIGDRVGVSCrpyhaCGECAECveedspd----sdpkgySVLCPTIKSqgi-giPGGFQEYVLVDARQLTII 215 Talaromyces sti...
EED51852      80 sa---lnwKVGDRVGILNfk-naCGNCAGCrgsrkr------ygkldPRFCDHRETggf-knDGCFAAYMVADSATTILL 148 Aspergillus fla...
EED55901      77 v----tgfQRGDRVGCINfd-svCGKCADCka-------------glPIYCDAPLMkgi-ttDGGWAEYMVADARFLVKL 137 Aspergillus fla...
EEU37448      79 v----rgiAVGQRVGVPGrayhpCGSCYECthpgh-------demgySPYCPQAGNlgl-trDGGFQEYCLVDSRQIAPL 146 Nectria haemato...
Feature 1                     #   #                     # ## #                 ##    #           
1LLU_H       141 PKNVEFAEIAPILCAGVTVYKGLKqt--nARPGQWVAISGIGG-LGHVAVQYARAMGlHVAAIDIDdAKLELARKLG--- 214 Pseudomonas aer...
EAL20142     143 PDSMSFEQAAPLGCAGVTVYAGIKka--qLKPGDVIAISGVGA-LGYLGVQFAKAIGlKVVAIDTRtEALELVRSLDnd- 218 Cryptococcus ne...
XP_760628    148 PDSIPFDQAAPLFCAGATVYGALLav--ePQKRQWLAMVGIGG-LGHLGVQYAKAMGcNVIAIDNRkEGLELTNKAPdh- 223 Ustilago maydis...
XP_772242    133 PENIDFAQAAAFMCSGATVYNGLKaa--kLPPKSVVGIYGIGA-LGSLGVQFAKAMGhQVIAIDNRpQPLEHIRALGsl- 208 Cryptococcus ne...
XP_001796102 182 PAELSAVETAPLMCAGITIYAALKrc--aLKPGERVGILGAGGgLGHLGLQYATHMGyKVLGIEAAsSPLRLAEDVAskl 259 Phaeosphaeria n...
XP_001903156 163 PDSISAVQGASLMCAGLTIYAAIKnc--gLERGQKVGIMGCGGgLGHLGLQFAAKMGlGVIGVDVAdEPLRVSGEIEetl 240 Podospora anser...
EED24059     216 PPGLSQVEAAPLMCAGITIYAAIKkt--gLKPGQRIGIIGCGGgLGHLGLQYATAMGlKVYGVENAdRPLALARGLKni- 292 Talaromyces sti...
EED51852     149 PDSVSFEQGAPLLCAGATAWGAINkarpfLRTGDMIGVVGIGG-LGQLGVQFAKALGyRTVAIDNRdASLQLTDDMPpe- 226 Aspergillus fla...
EED55901     138 PDDMEFKVAAPLMCAGISIYGGIVra--dVPKGGSVGIVGIGG-LGHLGTQIAKCMGyKVAAIDVKqSALDAVASYEh-- 212 Aspergillus fla...
EEU37448     147 PESLHATQAAPLMCAGLTIWSALQhe--rVRKAQRVAIIGAGGgLGHIGVQFAAYLGkDVLAVDAAdQALQLVRQVKtdl 224 Nectria haemato...
Feature 1                                                        ####  #                ###      
1LLU_H       215 ------ASLTVNArqe---dpVEAIQRd-----------iGGAHGVLVTAVSnsAFGQAIGMARrGGTIALVGLPpgdf- 273 Pseudomonas aer...
EAL20142     219 ----hrPDLVIDAskskaddaLNAIHGlrpsg----yrgwDGVDASVLTSPAipSYQYAADLTRsHGTLILLAQPtkv-- 288 Cryptococcus ne...
XP_760628    224 ----lkPDKTFLId------sEEAQKKcidelsssfyetnPGVDRVVINCEDrgLIKFSQQFLRkGGQLVDVGLPadgpf 293 Ustilago maydis...
XP_772242    209 -----sPDLTLSSdhtv-eegLKAIEEmfpe------kllRGVDAMLVTGPQahEFDYAFKMTAkHGVVVALSCCnqpmi 276 Cryptococcus ne...
XP_001796102 260 ----dnRPLIIDArtd---kaHDIVQRlgtedg-kkhtgeMGLDAIIVLPESqaAFDYGMSLLKtHGTCVVVSFPekgf- 330 Phaeosphaeria n...
XP_001903156 241 rqkeglSVRIVDArisraediVQEMGKedqrq----drsnMGLDAVIVLPESqrAMDYGVSLLRaHGRCILVSFPangf- 315 Podospora anser...
EED24059     293 -----sGATIIDSrtt---taAEAIAHistedk-rpipgqFGLDAVIILPESqkAFQYGVDMLKnHGKCVVVSFP----- 358 Talaromyces sti...
EED51852     227 ----lrPDFIINSthd---yaSEKILErt---------ggEGLAAVINCADSiaVNAWSLGLLRiGGVAVLLGLPpeqw- 289 Aspergillus fla...
EED55901     213 -----yPDVLILAtd-----tVEKSLEkidgv---tsseySGLDATVLATDHpaAFELAAALTRkHGTMVLLGQPekgi- 278 Aspergillus fla...
EEU37448     225 ----geAGKRVHIsdar-kedPETMKAlgdsit-giapteVGLDAVILLPESqrAFDMGMKLLRnHGTMVVVSFPkdkl- 297 Nectria haemato...
Feature 1                        ###                                             #     
1LLU_H       274 ptpIFDVVLKGLHIAGSIVGTRADLQEALDFAGEGLVKATIHPGKL-DDINQILDQMRAGqiEGRIVLEM 342 Pseudomonas aeruginosa
EAL20142     289 efqYPTFLARDITLRGSLHGNEVDLKDTVELAYKCNIKSEVKTFTI-NQHREMLDAVESDtwKGKAVLVF 357 Cryptococcus neoformans v...
XP_760628    294 eldSFAMNFKEQTIRGRLICTPEQCQDMINLHADNGCTTFIEKTYTiDQANDMAEHYNSKklQGRLCIVF 363 Ustilago maydis 521
XP_772242    277 eidWKDIVLRDIRVVGGFCATIEVTKELLELASMKNVKSKIRTFSL-DEVNDLLTVYHQPdmHGKLVVTF 345 Cryptococcus neoformans v...
XP_001796102 331 hisARDVVFRDIRIIGSLVGSNRTLREMLEFSAKHNIRALSKSYPL-GKLNELVEEYHKGs-GGKLVLDL 398 Phaeosphaeria nodorum SN15
XP_001903156 316 hisARDVVFRDISIRGSLVGSNRMLREMVMFAAEHNVQARIKSYPL-KKLNDLVLEYKKGk-GGKLVIDM 383 Podospora anserina DSM 980
EED24059     359 ---PQGFHMTDIHYVGNVSGSIKQLREMTAFSAKHGIKAQIEVFPL-EKLNDLVKAYLSGh-GGKLVIDY 423 Talaromyces stipitatus AT...
EED51852     290 rfdTHPIVFCELVLRGSYVASRGEVEEMMAIVDQHGVRSQLTVVTK-DDILRVPEMYLSRsfRGRLVVKF 358 Aspergillus flavus NRRL3357
EED55901     279 tmsYQTVIYKDIKLVGSLVADTAQAQELVELFHRNRLHVEITEWKM-EEAEQMRQWYCSGasSGKNVIVM 347 Aspergillus flavus NRRL3357
EEU37448     298 svsAHDLVFRDIALVGSLVGRNHQLREMLDFVVKHQVQVRVKTFPF-NRLNELEEQSHQGe-GGKLVIDM 365 Nectria haematococca mpVI...

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