Conserved Protein Domain Family
CAD_like

?
cd08296: CAD_like 
Cinnamyl alcohol dehydrogenases (CAD)
Cinnamyl alcohol dehydrogenases (CAD), members of the medium chain dehydrogenase/reductase family, reduce cinnamaldehydes to cinnamyl alcohols in the last step of monolignal metabolism in plant cells walls. CAD binds 2 zinc ions and is NADPH- dependent. CAD family members are also found in non-plant species, e.g. in yeast where they have an aldehyde reductase activity. The medium chain dehydrogenases/reductase (MDR)/zinc-dependent alcohol dehydrogenase-like family, which contains the zinc-dependent alcohol dehydrogenase (ADH-Zn) and related proteins, is a diverse group of proteins related to the first identified member, class I mammalian ADH. MDRs display a broad range of activities and are distinguished from the smaller short chain dehydrogenases (~ 250 amino acids vs. the ~ 350 amino acids of the MDR). The MDR proteins have 2 domains: a C-terminal NAD(P) binding-Rossmann fold domain of a beta-alpha form and an N-terminal catalytic domain with distant homology to GroES. The MDR group contains a host of activities, including the founding alcohol dehydrogenase (ADHs), quinone reductase, sorbitol dehydrogenase, formaldehyde dehydrogenase, butanediol DH, ketose reductase, cinnamyl reductase, and numerous others. The zinc-dependent alcohol dehydrogenases (ADHs) catalyze the NAD(P)(H)-dependent interconversion of alcohols to aldehydes or ketones. Active site zinc has a catalytic role, while structural zinc aids in stability. ADH-like proteins typically form dimers (typically higher plants, mammals) or tetramers (yeast, bacteria), and generally have 2 tightly bound zinc atoms per subunit. The active site zinc is coordinated by a histidine, two cysteines, and a water molecule. The second zinc seems to play a structural role, affects subunit interactions, and is typically coordinated by 4 cysteines.
Statistics
?
PSSM-Id: 176256
View PSSM: cd08296
Aligned: 24 rows
Threshold Bit Score: 479.433
Threshold Setting Gi: 118470700
Created: 2-Nov-2009
Updated: 2-Oct-2020
Structure
?
Aligned Rows:
 
Feature 1:putative NAD(P) binding site [chemical binding site]
Evidence:
  • Comment:MDR family binds NAD(P) as a cofactor
  • Comment:defined by comparison to MDR alcohol dehydrogenase like family

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
Feature 1                                               ###  #                                   
YP_001889385   4 MKAVQvakaggpleLVERDVPepg--agQVLIKIQACGICHSDVLTkegqwpgleyPRVPGHEIAGVIDTVGagv---eg 78  Burkholderia ph...
XP_389540      8 YKACVieka--napFTLKDVPlkhpskgQILVKVLACGVCFSDVGVseghm-gdvfPRVPGHEIIGDVVEVGedv---dn 81  Gibberella zeae...
EAK85593       8 YKAAVfekt--gqpLTFKDIElkhpedgQILVKVEACGVCHSDAHVqaga--fgplPRVPGHEIVGKVVEVGphv---tr 80  Ustilago maydis...
EED78875       4 YSAYGftkkggplePITFDWKdpq--pgEIVVKVLACGVCASDEAAkhqgwpgvkyPIVPGHEIVGEVAAIPste---sk 78  Postia placenta...
EED19785       7 FRQAVfkes--ggpLVIEDANlnppaqgEILVKVEACGVCYSDTIAqhnwm-gggfPQVPGHEIIGEVAAVGsgv---sg 80  Talaromyces sti...
EEH21186       9 YKVACfegk--seeLSIKSIPleypkhgEILVKVLACGVCHSDVLVkdgva-gnsyPIVPGHEIVGKVHAVGpsv---kn 82  Paracoccidioide...
EEU36929       8 FKAWVipsa--gvpIELRDVElkqpgagEILVKVRACGVCFTDVAVqqgnm-gdvfPRIPGHEIVGDVVAIGdgv---ng 81  Nectria haemato...
EAY56474       1 MKAVRslgp--gqpFVCQDVPdprpgsgEVLLRVQACGICHSDMFVreglfpgisyPRIPGHEVVGVILETGpgv---ad 75  Leptospirillum ...
YP_134692      5 MQAAVvpepgddfeVVERDVPepd--pgEVRVSVDACGICHSDVFAkegtnpvvsyPRVPGHEVAGHVDAVGdav---da 79  Haloarcula mari...
EED85179      11 YKGVScagphepweVITKELRapa--pnEVLLRVHASGICNSDHFVaegtwpgisyPRITGHEVVGRIAAVGsalegdar 88  Postia placenta...
Feature 1                                                                                   #   #
YP_001889385  79 WAAGQRVGVGWHGGHCGRCEHCrrgDFVLCQralvpGISYDGGYAEFMVAPveALARIPDd----LSDVDAAPLLCAGIT 154 Burkholderia ph...
XP_389540     82 FKNGDRVGGPWHGGHDRTCRQCqraQFQMCDhkevnGVSRDGGFAEYVLLRseAVVRVPKe----IEPADAAPLLCAGVT 157 Gibberella zeae...
EAK85593      81 WKSGDRVGGAWHGGHDGTCRQCnqgLFQMCDngqinGVTRDGGYAEYCLLQaeAAVRLPSd----GDAVQMAPLMCAGVT 156 Ustilago maydis...
EED78875      79 WKLGQVVGSGWHGGHCHLCAACrvgDYMMCEkqdvnGILRHGGYAQYATLRteAACAVPDg----MDPAEVAPLFCAGVT 154 Postia placenta...
EED19785      81 WAVGDRIGGGWHGGHDGTCKACktgHYQMCDnqlinGVTKVGGYAEYCILRseAAVRIPPd----VDASEYAPMLCAGVT 156 Talaromyces sti...
EEH21186      83 FKIGDRVGGPWHGGHDGICRACnrgDFQLCDsgeinGVTRPGGYAEYCILRveAVARVPKs----MDPAQIAPLLCAGMT 158 Paracoccidioide...
EEU36929      82 FSLGQRVGGAWLGGHDNVCRSCrrgLFQSCSnvvynGASRDGGYAEYVLLRseAAVSVPEd----IDPAEVAPLLCAGLT 157 Nectria haemato...
EAY56474      76 FRKGDAVGVGWHGGHCLTCPACrrgDFILCSrgritGISFDGGYAEFMVAPehALARIPDg----MDPAEAAPLLCAGVT 151 Leptospirillum ...
YP_134692     80 WDVGDRVGVGWHGGHCSTCDQCrqgNFLQCEngeitGLSYDGGYAEYMTAPseALAKIPEs----LDAAAAAPLLCAGVT 155 Haloarcula mari...
EED85179      89 FEVGALAGAGWNGGYCGCCEYCrqgEYWTCAtaavtGFTFDGGHAEYMYVPemAVVSLPEealqdASYAELAPLLCAGCT 168 Postia placenta...
Feature 1                           ######                 ##   #              #                 
YP_001889385 155 TFNALRnsgarAGDVVAVLGIGGLGHLGVQFARKMGFVTVAIARGqDKASLAKELGAHHYIDSttanvaqALQALGGARV 234 Burkholderia ph...
XP_389540    158 VFNGIRkmhveQGRIVAVQGLGGLGHLAVQYAYKMGYEVAVLSSTdDKADFAKKLGAHHYINTktsdvgeELNKLGGASI 237 Gibberella zeae...
EAK85593     157 VHNGIRkmnitPGEVVAIQGLGGLGHLAVQYARKMGYRTVALSRGtDKKDFAMKLGATDYIDTssqdaaeALQKMSGAAL 236 Ustilago maydis...
EED78875     155 MFNGLRnmsakPPDYVAIQGIGGLGHLGIQFAKAMGYRPIALSSSsAKEELSRKLGAEVYVDGskvdqaeALQELGGTKL 234 Postia placenta...
EED19785     157 VFNSMRhmqipPGSTVAIQGLGGLGHLALQYANKFGYRVVALSRDnKKEKFARQLGAHEYIDGskedigqALQRLGGASL 236 Talaromyces sti...
EEH21186     159 TFNGIRkmeitPGDLVAIHGLGGLGHLAVQFASKMGYKVAVISSKsGKEKFARELGAHHFIDSskqdiptALQEIGGAAL 238 Paracoccidioide...
EEU36929     158 VFNAIRkahieQGNLMVVQGIGGLGHMAIQYARKMGYKVAVVSSSaSKKDLALQLGAHVYIDTssedavkKLQELGGASL 237 Nectria haemato...
EAY56474     152 TFNSLRhagalAGSVVAVLGIGGLGHLALQFAKKMGFRTVALSSGsEKSGLARSLGADEYVDMkkenpveALEKMGGARV 231 Leptospirillum ...
YP_134692    156 TFNALRnsdanVGDLVAVQGVGGLGHLGIQYAHAAGFETAAISRTpEKESLAKELGADHFINAaetdagqRLQELGGADV 235 Haloarcula mari...
EED85179     169 AFGAITttkwkPGDLCLVQGIGGLGHLAVQFAARCGLKVYAVSSGsSKRDLALSLGAHEYIDSsttdvvsAIQALGGAQV 248 Postia placenta...
Feature 1           ## #                   ##                          ###                       
YP_001889385 235 ILATVTSgkaMSAVVGGLglNGKLIMVGLse----epVEVPiaqfimgrNSVQGWPSGTSADSQDTLAFSALSGIKPMIE 310 Burkholderia ph...
XP_389540    238 IVQTAPNpkaISPLIQGLapEGKLLNLAPvggaefdtVALVlk-----gASVVGWPSGHALDSEEALRFSLTHGVKCMVE 312 Gibberella zeae...
EAK85593     237 IVATAPNpehISPLVGGCkpMGKLLILAPvg-----dVRVNsiamitkgISVHGWPSGHALDSEDAVEFSQRFGVECMCE 311 Ustilago maydis...
EED78875     235 IMCTAPNadvIRTLLLGLavGGELCIVALage---apINLGalv--tkrWSIRGWPNGVAPDSEDCVKFAKVHGIRTMVQ 309 Postia placenta...
EED19785     237 IVSTAPDkdsITPLLSGLgiRGKLLILSVvdg---lpVNSAilm--rrgSSIHSWPSGHAMDSEEAIQFAGLQDIKCMVE 311 Talaromyces sti...
EEH21186     239 TLLSAPIpkyVQPLMKGLapRGKLLVLSPigef-tvdSALMir----raLSVSGWLTGHALDAEEAIDFARTHGIKCMVQ 313 Paracoccidioide...
EEU36929     238 ILATASNgksVSALTGAMqaGGKMVLVAAag-----pVEFNttdlivgtSAIQGWLTGNAQDAEDSIDFAKHFGVKCMIE 312 Nectria haemato...
EAY56474     232 ILATVPHsdtISRLVDALgdDGRMVLLGAdt----apVQVSplqlipkrKSVSGWPSGDALDSEETLNFANLAGIRAMVE 307 Leptospirillum ...
YP_134692    236 VLATAPSsdaISSIVSGIgvDGSVVVVGVpg----epVAVSgqqlvqtrGAVEGWASGHARDSQDTLEFSSLRDIAPEIE 311 Haloarcula mari...
EED85179     249 IICTAPYakhISAILPAVakNGTITLISAatd---gpVEVSnvlmnmnrATLRGFACGCAPDTEQCIRFSTLQGVKPMVQ 325 Postia placenta...
Feature 1                                  
YP_001889385 311 EFPLtkAAEAYDRMMSGaaRFRVVLN 336 Burkholderia phytofirmans PsJN
XP_389540    313 KYPLekVQDAVDSLKAGkpRFRNVLV 338 Gibberella zeae PH-1
EAK85593     312 TFPLakANEAFEHMMANkaRFRAVLT 337 Ustilago maydis 521
EED78875     310 KFPLekAEEAYNHRSTA--RFRAVII 333 Postia placenta Mad-698-R
EED19785     312 TFPLekANEAYGAMLNGsvRFRSVIK 337 Talaromyces stipitatus ATCC 10500
EEH21186     314 KYKLedVNKAMDDLVHArvRFRPPPN 339 Paracoccidioides brasiliensis Pb03
EEU36929     313 RFPFekAPEALERMTSGavRFRGVLT 338 Nectria haematococca mpVI 77-13-4
EAY56474     308 RMPLdrAEEAYEIMIRNkaRFRVVLT 333 Leptospirillum sp. Group II UBA
YP_134692    312 TYPLeeVNEAYGRMIDNeaRFRAVLE 337 Haloarcula marismortui ATCC 43049
EED85179     326 EFSVeqFTDAYTAVMANkaRFRNVVV 351 Postia placenta Mad-698-R

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap