2J3J


Conserved Protein Domain Family
double_bond_reductase_like

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cd08295: double_bond_reductase_like 
Click on image for an interactive view with Cn3D
Arabidopsis alkenal double bond reductase and leukotriene B4 12-hydroxydehydrogenase
This group includes proteins identified as the Arabidopsis alkenal double bond reductase and leukotriene B4 12-hydroxydehydrogenase. The Arabidopsis enzyme, a member of the medium chain dehydrogenase/reductase family, catalyzes the reduction of 7-8-double bond of phenylpropanal substrates as a plant defense mechanism. Prostaglandins and related eicosanoids (lipid mediators involved in host defense and inflamation) are metabolized by the oxidation of the 15(S)-hydroxyl group of the NAD+-dependent (type I 15-PGDH) 15-prostaglandin dehydrogenase (15-PGDH) followed by reduction by NADPH/NADH-dependent (type II 15-PGDH) delta-13 15-prostaglandin reductase (13-PGR) to 15-keto-13,14,-dihydroprostaglandins. 13-PGR is a bifunctional enzyme, since it also has leukotriene B(4) 12-hydroxydehydrogenase activity. Leukotriene B4 (LTB4) can be metabolized by LTB4 20-hydroxylase in inflamatory cells, and in other cells by bifunctional LTB4 12-HD/PGR. These 15-PGDH and related enzymes are members of the medium chain dehydrogenase/reductase family. The medium chain dehydrogenases/reductase (MDR)/zinc-dependent alcohol dehydrogenase-like family, which contains the zinc-dependent alcohol dehydrogenase (ADH-Zn) and related proteins, is a diverse group of proteins related to the first identified member, class I mammalian ADH. MDRs display a broad range of activities and are distinguished from the smaller short chain dehydrogenases (~ 250 amino acids vs. the ~ 350 amino acids of the MDR). The MDR proteins have 2 domains: a C-terminal NAD(P) binding-Rossmann fold domain of an beta-alpha form and an N-terminal catalytic domain with distant homology to GroES.
Statistics
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PSSM-Id: 176255
View PSSM: cd08295
Aligned: 12 rows
Threshold Bit Score: 526.502
Threshold Setting Gi: 147800900
Created: 1-May-2009
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 29 residues -Click on image for an interactive view with Cn3D
Feature 1:NAD binding site [chemical binding site]
Evidence:
  • Structure:2J3J; Arabidopsis thaliana Double bond reductase (At5g16970) binds NAP, contacts at 4A
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                              ##                         
2J3J_A          3 ATNKQVILKDYVSGFP-TESDFDFTTTTVElrvp---egtNSVLVKNLYLSCDPYMRIRMGKpdpst--aalaqAYTPGQ 76   synthetic con...
BAD35462        3 VESRYVAVRHHVEGLP-SEDDFEVKAARVRwwp-----esGEVLVRNLYLSVDPYQLNRMKRrsash---lavdGIVPGE 73   Japanese rice
ABG91753        5 VPNRELILVAYANEGPvTDSHLNIRETKLDlgsvgkdgssGDVAVQNLWISVDPYLRQLMKEsddg----lylpSFPLNQ 80   loblolly pine
ABK26403        6 VNNNQVILKDYITGMP-KLSHMLLKSTKLPlqlk---dgsQDVLAKNLYLSCDPYMRGCMRNlpsy-----ftpIFTPGS 76   Sitka spruce
CAN68146        6 VSSKRVMLRNYVTVFP-QETYLELSQGTIVlkvpq-gsnaFWSRISTCHAIHKCTIVCKKVKmmi------tssSLKPGS 77   wine grape
CAN68148        7 VRNKQVVLRDYVSGYL-KESDMCMATATITlkvp--egnsKAVLVKNLYLSCDPYIRGHTKSiqgg----yadeYFKPGS 79   wine grape
CAN60126       18 VESREWVMSAYAAEGVpTSDHLKLRTTKLSldan--sipdGHVAVELLWISVDPYLRTRMTGhddg----lyfsQFALNE 91   wine grape
XP_001759634    6 VRNKQIKLKHYVLGKL-EESDFQISSNEITlel----ndnNDVLVKNLYLSCDPYMRHRMRGetns-----yipPYQEGQ 75   Physcomitrell...
XP_001780006   11 VRHKQVVLAAIPKGMP-KVTDLRVEETETDvvvq---pdsNDVAVKLLYISLDPYYRELMAEedll-----glgLYKKGE 81   Physcomitrell...
EEC69038        6 VSNKRVILKRYVTGFP-SEEDMEVVIGDAPlmav--pagsEAVVVKNLYISCDPYMRNRMTRhevp----syvtDYVPGE 78   Indian rice
XP_002308086    3 VNNRYITITKHIQGSA-KESDFELKVAPLGlsve---pgsNDIIVKNLCVSIDPYQLNRMKSysssqktvqaadGISPGQ 78   black cottonwood
XP_002267164    6 VGNKQIILKNYTDQIP-KETDLELRHGNKIklkv--pkgsGAFLVKNLYLSCDPYMRGRMRDfhds-----ylpPFTPGS 77   wine grape
Feature 1                                                                         #   #           
2J3J_A         77 PIQGYGVSRIIESGHPDYKKGDLLWGI-VAWEEYSVItpm-thaHFKIQHtd-vpLSYYTGlLGMPGMTAYAGFYEVCSP 153  synthetic con...
BAD35462       74 RIDAYGAGEVVASACEEYKEGDVVAGV-LGWEDYTLFrpspgvlMSKLAAsddlpLSHHLSaLGTSGMTAYAGLYEVGRP 152  Japanese rice
ABG91753       81 AIRSILVGKVVASANPAFEVGDIVSGF-YQVSEYAIVpr---gdLMKIDTsv-vkPSDYLGlLGMPALTAWAGFIIVGEP 155  loblolly pine
ABK26403       77 VINGYGVAKVVISNHPDFKEDDLVMGI-TGWEEYSIIpgg--nqLQKIRYtd-ipLSYYLGiLGMPGLTAYVGFYDLSSP 152  Sitka spruce
CAN68146       78 AITGYGVARVLDSGDPNFREGELIWGT-TGWEEYSLItts--siRFKIQHtd-vpLSNYTGiFGMPGITAYARFYEICSP 153  wine grape
CAN68148       80 PIVGYGVAKVLDSGHPDFKKDDLVWGF-TGWEEYSLItat--esLFKIHHtd-vpLSYYTGlLSMPGMTAYTGFYEVCAP 155  wine grape
CAN60126       92 VITAFGVGRVXXSKNGKFSTGDIVXNPfFPFAEYCIApp---tfLRVIDPtagieLPAYLSsLGVPGFAAWVGIEVLGNP 168  wine grape
XP_001759634   76 VLDGYGVSKVVLSNNSSFKVGDCVSSW-TRWEEYSVIprg--qrLKVIDPal-apLSYHAGaLGMAGFTAYVGFFEVLKP 151  Physcomitrell...
XP_001780006   82 PIYGGVVAEVVLSDNPDFQVGDTVLGN-LNFSEYVVVpkg--qdLIKVDAsq-vpASYYLGvLGMPGLTAWVGLKLIGKA 157  Physcomitrell...
EEC69038       79 VLTNYGVMKVISSGHPDFKAGDLVWGI-TGWEGYTLIdnp--esLSKINHpd-lpLSYYTGvLGLPGLTAYAGFFEICKP 154  Indian rice
XP_002308086   79 PIDALGVAKVLVSDNPEFVKDDLVVGF-VHWGEYSVVkgg--gmLRKVDPktelpLSYHAGsLGLSGLTAYAGLFEICKP 155  black cottonwood
XP_002267164   78 VIEGFGVSRVVDSDNPNFKPGDLIAGV-TGWEEYSLIyrt--qqLRKIQHqddipLSFHVGlLGMPGFTAYAGFYEVCSP 154  wine grape
Feature 1                  # #####                 ##   #                                         
2J3J_A        154 KEGETVYVSAASGAVGQLVGQLAKMMGCYVVGSAGSKEKVDLLKTk---------------------------------- 199  synthetic con...
BAD35462      153 EAGEKVFVSAASGSVGSLVGQFAKLAGCYVVGCAGTNAKVDLLKNk---------------------------------- 198  Japanese rice
ABG91753      156 KPGDEVFVSAAAGSVGMLVGQLAKIKSCRVVGSAGSDQKVKLLKE----------------------------------- 200  loblolly pine
ABK26403      153 KAGEKVFISAASGAVGQLVGQFAKMHGCYVVGSAGSKQKVELLKDr---------------------------------- 198  Sitka spruce
CAN68146      154 KKGEHVSVSAASGAVGQLVGQFAKLMGCYVVGSVGSKEKVDLLKNk---------------------------------- 199  wine grape
CAN68148      156 KKEEYVFVSSAFGAVGQLVGQLAKLLGCYVVGSAGSKEKVDLLKTk---------------------------------- 201  wine grape
CAN60126      169 KPGSNVFISAAAGGVGIFAGQLAKLKGCRVVGSTGTDEKVKLLKEe---------------------------------- 214  wine grape
XP_001759634  152 KKGETLFVSAASGAVGQLVGQLARDAGLYVVGSAGSQEKIDLLTNk---------------------------------- 197  Physcomitrell...
XP_001780006  158 KAGDQVFVTAAAGAVGQVVGQLAKVYGCRVVGSAGSDEKVKLLKTe---------------------------------- 203  Physcomitrell...
EEC69038      155 KKGDYVFISAASGAVGQIVGQLAKITGCYVIGSAGSDEKCNHPEHnisrsrntcchwatgccslqgeassasavwlwaas 234  Indian rice
XP_002308086  156 KKGDKVFVSAACGSVGNLVGQYAKLSGCYVVGCAGSRDKVALLKEk---------------------------------- 201  black cottonwood
XP_002267164  155 QKGETVLVSAASGAVGQLVGQLAKLHGCYVVGSAGSSQKVDLLKNk---------------------------------- 200  wine grape
Feature 1                                                                            #            
2J3J_A        200 -----------------------------------------------------------FGFDDAFNYKeesDLTAALKR 220  synthetic con...
BAD35462      199 -----------------------------------------------------------LGFDDAFNYKdepDMKSALKR 219  Japanese rice
ABG91753      201 -----------------------------------------------------------FGFDDAFNYKcetDLDAALSR 221  loblolly pine
ABK26403      199 -----------------------------------------------------------LGFDDAFNYKeelDLHATLKR 219  Sitka spruce
CAN68146      200 -----------------------------------------------------------HGFNEAFNYKeehELDAALKR 220  wine grape
CAN68148      202 -----------------------------------------------------------IGFDEAFNYKeekDYDACLKR 222  wine grape
CAN60126      215 -----------------------------------------------------------FGYDEAFXYNketDFDAALXK 235  wine grape
XP_001759634  198 -----------------------------------------------------------LGYNAAFNYKeepDLVAAVAK 218  Physcomitrell...
XP_001780006  204 -----------------------------------------------------------FGFDDAYNYKteaDLDKALKR 224  Physcomitrell...
EEC69038      235 avwpvagvvrvgggvvggggaasvvwlwpasavwpvagvvrtvrvdgiggvvvnllktkFGFDDAFNYKkelDLDAALKR 314  Indian rice
XP_002308086  202 -----------------------------------------------------------LGFDDAFNYKeetDLNSALTR 222  black cottonwood
XP_002267164  201 -----------------------------------------------------------LGFDEAFNYKeepNLDAALKR 221  wine grape
Feature 1                    ##                    ##### #                          ###           
2J3J_A        221 cfPNGIDIYFENVGGKmLDAVLVNMNMHGRIAVCGMISQYNLenqe--gvhNLSNIIYKR-NRIQGFVVSDFYdk-ySKF 296  synthetic con...
BAD35462      220 yfPDGIDIYFDNVGGEtLEAALANMNTYGRVALCGVISEYTDaghr--avpDLLEVIYKR-ITIRGFFAWDFLtr-fAEF 295  Japanese rice
ABG91753      222 yfPRGIDIYFDNVGGRmLEAVLNHINMKARIPLCGMISQYNQewkqrfgvrNLLNLVGKC-AKMEGFMSGQYHhr-mGEF 299  loblolly pine
ABK26403      220 yfPNGIDIYFENVGSKmLEAVLENMNLHGRIAACGMISEYNLeegq--girNISRVISKR-IKIQGFIIHDYWhm-yPQF 295  Sitka spruce
CAN68146      221 cfPEGIDIYFDNVDGK-MLDAILNMRTRGRIAACGMVSQYNLeqpe--sikNLINIISKQ-VCIQGFVSHEQYhl-yPQM 295  wine grape
CAN68148      223 yfPEGIDIYFDSVGGKmLDAALLNMRLDGRIAACVLRKLYVSy-------sNLFILVMVSePEQQTTMAGSQKa---SGS 292  wine grape
CAN60126      236 yfPNGIDLYLDNVGGKmLEAVLNHVNLHARIPICGMISQYNQiwteregvrNLLNMVGKE-VRMEGYLVGSYLdr-fDDF 313  wine grape
XP_001759634  219 ycPQGVDKYFENVGGKtLDAVLDNMNNFGRVAVCGLISQYDQggqd--gvyNLKRIINKR-VTLQGFLQSDYLhl-ePKF 294  Physcomitrell...
XP_001780006  225 hlPNGIDIYFDNVGGEtLDAVFKNVNMGARIVLCGAISQYNVdkry--gikNLFSAVAKA-VKMEGFLVGKYTaehmGEY 301  Physcomitrell...
EEC69038      315 yfPEGIDIYFENVGGAtLDAVLPNMRLRGRIAACGMISQYNLanpd--gvhNLFYIVTKR-LRMEGFLVFDYNem-yHRF 390  Indian rice
XP_002308086  223 yfPDGIDIYFDNVGADmLEAAVANMNPFGRVAACGTIAEYSEtakr--aapNMIDVIYKR-IKIQGFLAMDHKsl-hSDF 298  black cottonwood
XP_002267164  222 yfPKGIDIFFDNVGGDmLDAALLNMRIHGRIAICGMVSQTSL---------SNPQGIHNY-KQGKIVYIEDMNqa-lESA 290  wine grape
Feature 1                                         ##   # #      
2J3J_A        297 LEFVLPHIreGKITYVEDVADGLEKAPEALVGLFhGKNVGKQVVVV 342  synthetic construct
BAD35462      296 TGVISDWIrqGKVQVIEDISDGLESVPSAFAALFsGDNIGKKMVKL 341  Japanese rice
ABG91753      300 FEEMTGYIkqGKIKYKEDVKVGLDSFLEAFNSMFtGENIGKPVIYL 345  loblolly pine
ABK26403      296 VEKVRGSIkeGKIVYVEDIADGLENAPAAFVGLFeGKNIGKQVVRI 341  Sitka spruce
CAN68146      296 LDTLLPYIreGKLVYVEDIAEGLERSPAALVGLFsGHNVGKQVAAV 341  wine grape
CAN68148      293 ENKTIDPS--HPLYIHHSDQPGHVLVPIKLNGVN-YQSWSKAVIHA 335  wine grape
CAN60126      314 TKEMETYTkeGKLRSKHKIYCGIESFIESLGSMFtSSNIGKVITTF 359  wine grape
XP_001759634  295 MDHMSKLIkaDKLVYFEDFAEGLDNAPNAFCRMMiGSKIGKQVITV 340  Physcomitrella patens subsp. patens
XP_001780006  302 ATEMSGYLkeGKVKYREHVTKGIENFPSAFAGLMtGENVGKSVLRV 347  Physcomitrella patens subsp. patens
EEC69038      391 EEETAAYLkeGKITYVEDVVVGLDAAPAALIGLFtGRNVGKQLVAV 436  Indian rice
XP_002308086  299 LSTTTEYIqnGKIKVQEDISIGVESIPLAFIGLFrGDNVGKKIVKI 344  black cottonwood
XP_002267164  291 PAAFVGLFsgKNVGKQVICSLSLTKTTPTISVCY-ATETDIYCLKT 335  wine grape

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