Conserved Protein Domain Family
ETR_like_2

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cd08292: ETR_like_2 
2-enoyl thioester reductase (ETR) like proteins, child 2
2-enoyl thioester reductase (ETR) like proteins. ETR catalyzes the NADPH-dependent conversion of trans-2-enoyl acyl carrier protein/coenzyme A (ACP/CoA) to acyl-(ACP/CoA) in fatty acid synthesis. 2-enoyl thioester reductase activity has been linked in Candida tropicalis as essential in maintaining mitiochondrial respiratory function. This ETR family is a part of the medium chain dehydrogenase/reductase family, but lack the zinc coordination sites characteristic of the 2-enoyl thioester reductase (ETR) like proteins. ETR catalyzes the NADPH-dependent dependent conversion of trans-2-enoyl acyl carrier protein/coenzyme A (ACP/CoA) to acyl-(ACP/CoA) in fatty acid synthesis. 2-enoyl thioester reductase activity has been linked in Candida tropicalis as essential in maintaining mitiochondrial respiratory function. This ETR family is a part of the medium chain dehydrogenase/reductase family, but lack the zinc coordination sites characteristic of the alcohol dehydrogenases in this family. NAD(P)(H)-dependent oxidoreductases are the major enzymes in the interconversion of alcohols and aldehydes, or ketones. Alcohol dehydrogenase in the liver converts ethanol and NAD+ to acetaldehyde and NADH, while in yeast and some other microorganisms ADH catalyzes the conversion acetaldehyde to ethanol in alcoholic fermentation. ADH is a member of the medium chain alcohol dehydrogenase family (MDR), which has a NAD(P)(H)-binding domain in a Rossmann fold of a beta-alpha form. The NAD(H)-binding region is comprised of 2 structurally similar halves, each of which contacts a mononucleotide. The N-terminal catalytic domain has a distant homology to GroES. These proteins typically form dimers (typically higher plants, mammals) or tetramers (yeast, bacteria), and have 2 tightly bound zinc atoms per subunit, a catalytic zinc at the active site, and a structural zinc in a lobe of the catalytic domain. NAD(H) binding occurs in the cleft between the catalytic and coenzyme-binding domains, at the active site, and coenzyme binding induces a conformational closing of this cleft. Coenzyme binding typically precedes and contributes to substrate binding. Candida tropicalis enoyl thioester reductase (Etr1p) catalyzes the NADPH-dependent reduction of trans-2-enoyl thioesters in mitochondrial fatty acid synthesis. Etr1p forms homodimers, with each subunit containing a nucleotide-binding Rossmann fold domain and a catalytic domain.
Statistics
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PSSM-Id: 176252
View PSSM: cd08292
Aligned: 13 rows
Threshold Bit Score: 375.903
Threshold Setting Gi: 167621764
Created: 1-May-2009
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
putative NAD(P)putative dimer
Feature 1:putative NAD(P) binding site [chemical binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                  #                                     
NP_602267      1 MRAITHNTFGDPadVLQITEKEIp-TPGPGQVRIQVTLATIHNHDLWTVk--GSYGFVPDL---PAAAGTEAVGIVDALG 74  Corynebacterium...
YP_371957      1 MRTAIHQSMGQPeqVLEIRNVARp-QPQAGEVLLQMILAPIHNHDLMQIa--GTYGIKPEL---PARAGTEAVGRVLAVG 74  Burkholderia sp...
ABO13682       1 MRSIIHRNFGEPvdVLEQADMPKp-EPKAGEVRIKTIMSPIHNHDVWTVr--GSYGYKPTL---PAIGGSEAVGIVDALG 74  Acinetobacter b...
YP_747291      1 MRASIYHQFGEPskVLNEEEVVLp-SPGVNQVRVKTILSPIHNHDLWTVr--GTYGHKPNL---PAIGGSEALGIIDAVG 74  Nitrosomonas eu...
YP_263320      1 MRSATYDHFGKPteVLSIGDRPTp-EPKANQVRVKTILASIHNHDLLTIr--GKYGFKPEM---PAIGGSEAVGIIDAVG 74  Psychrobacter a...
XP_001921540  10 MRVIEYKAFGDPaeVLVSGERPMp-EPGPGEIRVRMTLSPIHNHDLWTIr--GSYGVKPPL---PAIGGTEAAGVVDAVG 83  zebrafish
EEB84250       1 MKAALHDTFGEPidVLKTTETDKp-TPAAGEVLVKMTLSPIHNHDLWTIr--GTYGYKPELp--GVIGGSEALGTIEAVG 75  Roseobacter sp....
NP_821881      2 NRRVVYTRGGSPanVLTVIEEPEp-APGSGQVLIRTTAFPVHPGDLQAV---EAYPEEATK---PVPAGVEATGVVEAIG 74  Streptomyces av...
YP_001477405   1 MRIAVHESLGQPeqVMSIQETSRp-TLKAGEVLLQMVLSPIHNHDLMQIs--GTYGTKPTL---PARAGTEALGRVLEVG 74  Serratia protea...
NP_396144      1 MRAAVYFKHGRPedVLQAVEVEDlkRPAHNEVLIRVLLRPVHHGDLLGIs--GRYQPGAVVsreGVRVGFEGYGLVEEAG 78  Agrobacterium t...
YP_001676549   1 MRSIVHTQHGDPdqVLSVAKVAPp-TPGPGQVLVRVLRTPVHFGDLLGVsasPAFGSPPAIgptGRTPGFEGAGVIEALG 79  Caulobacter sp....
NP_639466     64 MRAAIHTQFGDPakVLELGERPTp-QPGKGQVRVAMRRAPIHNHDLWTVr--GNYGYKPEL---PAIGGSEAAGVIDALG 137 Xanthomonas cam...
ZP_03544247    1 MRSAIHTTFGEPseVLELIDRPIp-QPGPGEVRIKTLLAPIHNHDLWTIr--GSYGYRPEL---PAIGGSEAVGIVDALG 74  Comamonas testo...
Feature 1                                                           ##  #                        
NP_602267     75 EGVeg---lQVGQRVASGtsfGIWAEYALVDASGLIPVPEqLSDESAAQLVAMPFSAISLLDFLDmk------pGEWLIQ 145 Corynebacterium...
YP_371957     75 EGVth---lQVGQRVSVSgafGTWADAFVAPADQVLPVPDgISDELAAQLLIMPTSAMVVLDDLGvq------sGQWMML 145 Burkholderia sp...
ABO13682      75 EGVeh---vQVGQRIAVAavhGSWAEYFIAPAQGIIPLNNeIDDETAAQLIGMPISALMLLDFVNvq------pGQWLIQ 145 Acinetobacter b...
YP_747291     75 DGVke---lTLGQRVVGSpimGVWAEYFLAPAEILTIVPDtISDEVGAQLASMPFSALLLLEFLNae------aGQWIIQ 145 Nitrosomonas eu...
YP_263320     75 SDVke---lKVGQRVAAAsvqATWAEYFVAVEDMVFPMPDsLDDEMAAQLIAMPLSALMLLEFLDlq------aGQWVIQ 145 Psychrobacter a...
XP_001921540  84 DGVtn---vSVGQRVMAAgltDTWAEYFLMNAQRAIPLPDsVPDELGCQLIAMPISALMALDDLGvk------pGEWLIQ 154 zebrafish
EEB84250      76 EGVda---aMIGKRVTIAgvhGTWAEYFVAPADGVLPLPDaISDTAGAQLIAMPFSAISLLETLKak------kGDWIIQ 146 Roseobacter sp....
NP_821881     75 PGTrvapgvTVGGRVTVFpqpGAWSQWIVADADALVAVPDeLSDEVAAQMLVNPLTTVMLRREAQehpa--fgyDGLLVQ 152 Streptomyces av...
YP_001477405  75 EGVkd---lQIGQRVAASgafGTWADAFVAPADQLLPIPDgISDELAAQLLVMPASATVVLDDLGvk------sGQWMVL 145 Serratia protea...
NP_396144     79 DGVd----lRQGTRVAFFpgrGAWSEKAIVSAEYVTEIPDkVSDEAAAQLHINPLTAALLLRAVEasga--kpgRDVVVL 152 Agrobacterium t...
YP_001676549  80 EGVqlapglAVGSNVAFFpvsGAWSELIAVPVTSLVALPDgVSLNVAAMALVNTITASLILRAGHaavpegqhdDVTVIQ 159 Caulobacter sp....
NP_639466    138 EGVeg---lQVGQRVVAAgvhEAWAEYFLADASGVVPLPDgLDDERGCQLIAMPLSALMLIEFLQvk------qGDWIVQ 208 Xanthomonas cam...
ZP_03544247   75 EGVig---pTVGQRVAVAgvhNTWAEYFLAPANVLVPMPPaIPDEAAAQIIAMPLSALMLLEFLNve------pGQWIVQ 145 Comamonas testo...
Feature 1         # ####                   #                                           ##        
NP_602267    146 NSANGAVGRMLAQLAESRGIHVVGLVRRdaGVQELAAQ-NISGVVSTEtpgWEKQVEDITGGasiavALDSVGGSSAADL 224 Corynebacterium...
YP_371957    146 NAAAGAVGKNVALLAAARNIRVIALVNQaaQVEELRKL-GVDVVENTTvdgWQARIKTALNGepllhALDSVAGSLTGEM 224 Burkholderia sp...
ABO13682     146 NTANGAVGKTVAMIAQARGFPVINLVRRsdAIAEMQAL-GIQHVVATDqpnWKEQVKQIHGDqpliaGVDSIGGTASGEM 224 Acinetobacter b...
YP_747291    146 NAAGGAIGKTVSILAREQGVNTISLVRNseTEKELTAQ-GIEHVVSTAnqdWQQSVNELTAGasisyAIDSVGGAATAAL 224 Nitrosomonas eu...
YP_263320    146 NAANGAVGKSLAMLAAARGVKTINVVRSsdAVAELEAI-GVENNINTEeddWKDQVKSILGDdkisaAVDSVGGEDSGAL 224 Psychrobacter a...
XP_001921540 155 NAATGAVGKTIAMICQARGINVINVVRRdaGVGELEAL-GISNNVSTQdpgWMDKVKMFPGSdpilyAIDSVGAKESGQL 233 zebrafish
EEB84250     147 TAANGAVGKIMVTLAKARGINLLSLVRRqeAAKELTDM-GIENVLSTSdadWQKKARDIVGAggavsAIDSVGGDVSADL 225 Roseobacter sp....
NP_821881    153 TAAGSSVGRLLTGASLVHNLALVNVVRSdrGAAELRKRfPDVPVVATEhpgWADEVREAAGGrpvsvALDPIGGKLAESL 232 Streptomyces av...
YP_001477405 146 SAAAGAVGKNLALLAASRQIRVIGLVNRdsQVKELRAL-GVDLVENTEkdgWQQRIKTALNGeallyGLDSVAGELTGEM 224 Serratia protea...
NP_396144    153 TAAGSAVARLVIGLLLDRGFDVVGIVRReaGVNEINVVfPDLPVVSTDkpgWQDKVVAAADGhpigvVLDPVGGETASAV 232 Agrobacterium t...
YP_001676549 160 TGAGSAVGRLITVLLAERGVKSLRLVRTaeSAGKLAKSlPGGPVFATElpdWVTKVREATQGkpihvALDGVGGKTLPGL 239 Caulobacter sp....
NP_639466    209 NTANGAVGKTVAMLAAARGINVINLVRRdaGVDELKAL-GIGNAISTAqagWQDKVRALAGDapivrAIDPVAGNAAGEL 287 Xanthomonas cam...
ZP_03544247  146 NTANGAVGKTLAMLAKARGVHVLNLVRRdaGVQELAAL-GIGNALSTAqpdWQERARSILGGslaraAVDSIGGSASASL 224 Comamonas testo...
Feature 1                    ## #                       ###                                      
NP_602267    225 VKLLgEGGTLVSFGAMGnpiMEIPSGPVIFKH--ITVKGFWGSKVsremp--aekkTQLFGELIARILdGTLTLPVDst- 299 Corynebacterium...
YP_371957    225 LHVMdDNATLVVFGALSnqpLNIDFQDVLFKQ--ATVRGFWGLRKvekls--dayrARMVAEILAIAQrNGFNLPVAav- 299 Burkholderia sp...
ABO13682     225 LNLLsENSLLVSFGSMTgetMQISSGDLIFKQ--ATVKGFWASVVnkemp--aarkKELIVELLTLATqKKLILPVEgvf 300 Acinetobacter b...
YP_747291    225 TALLgENGLLVSFGSMSgepMHIPAADIAFKQ--ITVKGFWQKKInqsma--vekrKSMMEKLICLVEtGKLKLPVDviy 300 Nitrosomonas eu...
YP_263320    225 LSLLgHGGTFAVFGAMSgkpMMLNPTDMIFKQ--ATLKGFWGSKLsqems--venkQRLVDELIERAVnGQLKLPTEat- 299 Psychrobacter a...
XP_001921540 234 MSLLgEGATLMSFGTMSggnMEISSGDLIFKQ--AKVIGFWGAKRsgkta--padfVRMIGELITLASnGQLNLPVEdi- 308 zebrafish
EEB84250     226 VDLLgTDGELVVFGTATgapMPLSSGALIMKH--ITVKGFWGARVskdmd--peerKRLITELVTLTAkGELVLEDGga- 300 Roseobacter sp....
NP_821881    233 LDLLtPGGKLVSYGQIGeepISVHASTLLHKS--LTLRGKNIGRWlseasadrrasDVATAKLIALTLkDQFDVAATyg- 309 Streptomyces av...
YP_001477405 225 LSVMnDNATVVVFGALSnhpLRIDFQDVIFKQ--ATVRGFWGLRKfaaln--neykLRMISEIMTMALrDGFSLPVAai- 299 Serratia protea...
NP_396144    233 IGLLaQGGSLVSYGDLSg--QPISVPALYFSTrdIRLSGVTVGRWaglp---veirKADIKLALELAAsRPELFQVEqa- 306 Agrobacterium t...
YP_001676549 240 AAILaDGGTVISYGSLGg--GDTDIRLLSARA--LTLRGLSIGRWgrea---eavrQADIATALRLAQtQSDLFDTAgv- 311 Caulobacter sp....
NP_639466    288 MALLaEGGELISFGSMTgepLQISSGDVIFKQ--ATVRGFWGSKVmqatk--aedkRRMIGELLTAALdGSLALPVEav- 362 Xanthomonas cam...
ZP_03544247  225 LSLLgEEGTLVSFGTMAgepMQIDSGSLIFKQ--ATVKGFWGSKVsqamt--pdnkRRLIGELLQRVLsGELKLPAEai- 299 Comamonas testo...
Feature 1                                   
NP_602267    300 --fDAADIVSAvrassePGRAGKVLIR 324 Corynebacterium glutamicum ATCC 13032
YP_371957    300 --hDLGDVASAsg---iRSSAGKVLLR 321 Burkholderia sp. 383
ABO13682     301 sfdEIKTAAQRat---qGARQGKVLLK 324 Acinetobacter baumannii ATCC 17978
YP_747291    301 pmnDVKNAATAsl---qSSRKGKVLLR 324 Nitrosomonas eutropha C91
YP_263320    300 --fDLSDILKAvdgkmqSEKNGKVLLK 324 Psychrobacter arcticus 273-4
XP_001921540 309 --yGLEDGAKAaaasmePGRKGKVLIR 333 zebrafish
EEB84250     301 --fALDDVAQAmtaaltPGRAGKVMIR 325 Roseobacter sp. GAI101
NP_821881    310 -ldELGNAVEHav---rPGKIGTVLVR 332 Streptomyces avermitilis MA-4680
YP_001477405 300 --yDLDDIEQAig---tKHGPGKVLLR 321 Serratia proteamaculans 568
NP_396144    307 --fDLADVGKAaalverPGKSGTILIA 331 Agrobacterium tumefaciens str. C58
YP_001676549 312 --yAPEQIGEAvahvarPGKTGTVLLS 336 Caulobacter sp. K31
NP_639466    363 --fDLHDAAKAaaasdkPGRSGKILLR 387 Xanthomonas campestris pv. campestris str. ATCC 33913
ZP_03544247  300 --fDLNDAAQAaaasmqPGRKGKVLLR 324 Comamonas testosteroni KF-1

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