1GYR,1GU7,1N9G,1N9G,1N9G,1ZSY


Conserved Protein Domain Family
ETR

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cd08290: ETR 
Click on image for an interactive view with Cn3D
2-enoyl thioester reductase (ETR)
2-enoyl thioester reductase (ETR) catalyzes the NADPH-dependent conversion of trans-2-enoyl acyl carrier protein/coenzyme A (ACP/CoA) to acyl-(ACP/CoA) in fatty acid synthesis. 2-enoyl thioester reductase activity has been linked in Candida tropicalis as essential in maintaining mitiochondrial respiratory function. This ETR family is a part of the medium chain dehydrogenase/reductase family, but lack the zinc coordination sites characteristic of the alcohol dehydrogenases in this family. NAD(P)(H)-dependent oxidoreductases are the major enzymes in the interconversion of alcohols and aldehydes, or ketones. Alcohol dehydrogenase in the liver converts ethanol and NAD+ to acetaldehyde and NADH, while in yeast and some other microorganisms ADH catalyzes the conversion acetaldehyde to ethanol in alcoholic fermentation. ADH is a member of the medium chain alcohol dehydrogenase family (MDR), which has a NAD(P)(H)-binding domain in a Rossmann fold of a beta-alpha form. The NAD(H)-binding region is comprised of 2 structurally similar halves, each of which contacts a mononucleotide. The N-terminal catalytic domain has a distant homology to GroES. These proteins typically form dimers (typically higher plants, mammals) or tetramers (yeast, bacteria), and have 2 tightly bound zinc atoms per subunit, a catalytic zinc at the active site, and a structural zinc in a lobe of the catalytic domain. NAD(H) binding occurs in the cleft between the catalytic and coenzyme-binding domains, at the active site, and coenzyme binding induces a conformational closing of this cleft. Coenzyme binding typically precedes and contributes to substrate binding. Candida tropicalis enoyl thioester reductase (Etr1p) catalyzes the NADPH-dependent reduction of trans-2-enoyl thioesters in mitochondrial fatty acid synthesis. Etr1p forms homodimers, with each subunit containing a nucleotide-binding Rossmann fold domain and a catalytic domain.
Statistics
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PSSM-Id: 176250
View PSSM: cd08290
Aligned: 56 rows
Threshold Bit Score: 210.154
Threshold Setting Gi: 159483643
Created: 1-May-2009
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
NADP bindingdimer interface
Conserved site includes 20 residues -Click on image for an interactive view with Cn3D
Feature 1:NADP binding site [chemical binding site]
Evidence:
  • Structure:1N9G: Candida tropicalis mitochondrial 2-enoyl thioester reductase binds NADP, contacts at 4A
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                          #                             
1GYR_A         4 AQAVLYTQHGEPk--dVLFTQSFEIDDDnla-----pnEVIVKTLGSPVNPSDINQIQGVNPSKPakttgf-gttepAAP 75  Candida tropicalis
1N9G_A        26 AQAVLYTQHGEPk--dVLFTQSFEIDDDnla-----pnEVIVKTLGSPINPSDINQIQGVYPSKPakttgf-gtaepAAP 97  Candida tropicalis
XP_001212124   3 AQTITFQQHSTEps-rVIRVHHHESVGDrpl----ppdSVLLRLLAAPINPQDLLVIAGRYPVQPhyk------yadEPI 71  Aspergillus ter...
XP_001226017  34 ASAVDPTSTASD----AGQGKGEEGKDEg---------EVLVRFLAAPVNRVDLMLLAGRYPIKPkytapspdgttrHPI 100 Chaetomium glob...
XP_001230010   5 QVVLTYSATSNSpa-eVIQAHHDQDGPVpgdekplpeaTALVTFLVAPINPQDVMAIAGRYPVKPeyr------hldNPI 77  Chaetomium glob...
XP_001463016   6 SAGWRYARCGPIs--qVLAYEMFDITPGk--------eEAVVQMATAPLHRVDAAVVNGTALGRKrvnm-----asfPRI 70  Leishmania infa...
YP_001260825   3 PPANIGLLAGTAd---GVRFEPQAMPDApg------pdGLTVRMLFAPVNPADLLAIDGGYAFALa----------aDDP 63  Sphingomonas wi...
XP_001699870 286 VLQVVCRGVGGVqlaeALALQAAPLPAEle------wgHVLVAMRFAPVSAADLYTARLGGVYGAdta------pqvPYT 353 Chlamydomonas r...
CAP95067       1 MAESLVFTYGAPkpidGMHLVTQAVPTCgp-------eQVVVEFLAAPVNPLDFLVIHGKYPIKPqttimg-qdgeqRAV 72  Penicillium chr...
EEH08365       2 PLALTFAKPSWQaa-eALLLENYPEPEPkd-------nEVLIEFLAAPVNHLDLLVIAGGYPIKPkfq------lngNHV 67  Ajellomyces cap...
Feature 1                                                                                        
1GYR_A        76 CGNEGLFEVIKVGSNVss--------lEAGDWVIPShvnfGTWRTHALGnDDDFIKLPNpaqskan---gkpngltINQG 144 Candida tropicalis
1N9G_A        98 CGNEGLFEVIKVGSNVss--------lEAGDWVIPShvnfGTWRTHALGnDDDFIKLPNpaqskan---gkpngltINQG 166 Candida tropicalis
XP_001212124  72 PGYDGVARVERIGANVtt--------lQPGDHVIPRshglGTWRSEAVVpATSVLKVSNrl--------------dPTTA 129 Aspergillus ter...
XP_001226017 101 PGFDGCALVQSSSTPLf----------APGDLVLPGdlglGTWRTHAVLpASALIKLPAgv--------------dPIDA 156 Chaetomium glob...
XP_001230010  78 PGNDGVARVEATNPPPpttttnhnplpQPGDLVIPQrhglGTWRRHAILpLSALTRLPLpnmnplttptptatstpSAMI 157 Chaetomium glob...
XP_001463016  71 GGCEGVGKVVRAPAAAaatp----spvKEGDTVWVApl-hGTWATNIAVpVSQLHKIDPr----------------QAEM 129 Leishmania infa...
YP_001260825  64 LGAEGVGVVEQAGSRVsd--------lGPGDLVLPLd--rGNWTRYRAVaRDRVLAVPPgv--------------dPRQA 119 Sphingomonas wi...
XP_001699870 354 AGHDGVGVVVKVGPGVka--------lCEGDLVLPLrpfaGTWATAAVWhQRALIRLPQggy------------glPLEL 413 Chlamydomonas r...
CAP95067      73 PGSDGAARIVQIGSAVsn--------lAVDDLVILRthckGTWRTHAVFgEDDLIRIPStv--------------kPHLA 130 Penicillium chr...
EEH08365      68 GGFDGVGRVLKCGKDVtk--------lTPSDLVIPKalglGTWRTHATLiADDLIVIPPtp--------------dVTFA 125 Ajellomyces cap...
Feature 1            ##  #                              # ####                   #               
1GYR_A       145 ATISVNPLTAYLMLTHYvkl---------tpgKDWFIQNGGTSAVGKYASQIGKLLNFNSISVIRDrpn----------- 204 Candida tropicalis
1N9G_A       167 ATISVNPLTAYLMLTHYvkl---------tpgKDWFIQNGGTSAVGKYASQIGKLLNFNSISVIRDrpn----------- 226 Candida tropicalis
XP_001212124 130 GLLKMGCSTAYLLLESSnal----------qpGDLVAINAASGWIARMVVQFARLRGCGSICIIRDrdd----------- 188 Aspergillus ter...
XP_001226017 157 ALIRSGALIARLLLDEVtpl----------raGDWVIASAGTSGVSQFLVQLARQRGVKVVLVVRDrgep---------- 216 Chaetomium glob...
XP_001230010 158 PHITIDPIAASMLRTVFlpaylltedmralrpGDWVVQNAAGSTVAQVVAQFVRRKGARVVAVVRE-------------- 223 Chaetomium glob...
XP_001463016 130 AATASNFLVAQQLLDGYarl----------qkGDIVIQNGGSSLTSLAVSALAKAYGVKVLTAATPsar----------- 188 Leishmania infa...
YP_001260825 120 AMMRINPATAWLLLAASga-----------gpGDCLVQNAAGSTVAHWVRRLAALRDVAVIDVVRP-------------- 174 Sphingomonas wi...
XP_001699870 414 LALSREMVVAYTLLEGAgl-----------rpGDAVILNAANSSVGQVLLQLARLLRLRTVAVVRPpaqphaaaaaaaat 482 Chlamydomonas r...
CAP95067     131 SILRMGIAPAYFLLREYhgl----------epGDWIIQNAATGTISHFVSQLAPLYGIRVISVIRNrsta---------- 190 Penicillium chr...
EEH08365     126 AILKTCVLPAYLLLEDMkql----------kpGDWIIQNAGLGAISQMISQLAHLRGVKVISVIRD-------------- 181 Ajellomyces cap...
Feature 1                                                                                        
1GYR_A       205 ------ldEVVASLKEL------GATQVITEDqnn-------------------------sreFGPTIKEWikqs----- 242 Candida tropicalis
1N9G_A       227 ------ldEVVASLKEL------GATQVITEDqnn-------------------------skeFGPTIKEWikqs----- 264 Candida tropicalis
XP_001212124 189 ------veTTRQSLLSH------GAHVVLTESq-----------------------------lAQEGVAAAqtgg----- 222 Aspergillus ter...
XP_001226017 217 -----vlaVVKAELRAL------GADAVLSEGele-------------------------aelAARSSKAPgpsaaag-- 258 Chaetomium glob...
XP_001230010 224 --------RERERDGGCggggmaDVDVVLTERevre-----------------------ggvgASGELKAAaer------ 266 Chaetomium glob...
XP_001463016 189 ------faDAKQRHAKY------GSDVFEYNGa------------------------------GSRAMQAAvgk------ 220 Leishmania infa...
YP_001260825 175 --------GASAPGLAD------DEHLEAAVK-------------------------------AASGGRRV--------- 200 Sphingomonas wi...
XP_001699870 483 aghkgppaPAAARSATMtp---sGAASAAGTTtasgsgggagagggsgdskwdrtaawlrglgATEVLKDEgslraeldr 559 Chlamydomonas r...
CAP95067     191 ----deleRTKRSLRSH------GASLVLTEDel----------------------------rSTDALAGKr-------- 224 Penicillium chr...
EEH08365     182 --------RSPGTAWNT------TADIVLNESel----------------------------pNAEILKGKr-------- 211 Ajellomyces cap...
Feature 1                    ##                     ####                          ###            
1GYR_A       243 ---ggeaKLALNCVGGKSSTGIARKLn-NNGLMLTYGGMSFq----pVTIPTSLYIFKN-FTSAGFWVTELlk-nnKELK 312 Candida tropicalis
1N9G_A       265 ---ggeaKLALNCVGGKSSTGIARKLn-NNGLMLTYGGMSFq----pVTIPTSLYIFKN-FTSAGFWVTELlk-nnKELK 334 Candida tropicalis
XP_001212124 223 ----rrvMLALDAVFGASGQRLASLLs-TGGTYINYGSLGGaa--gqIILTQELLFWKQ-ITFRNFRLSQAlarypEEAQ 294 Aspergillus ter...
XP_001226017 259 llpkepiVLALDSVFGRVGQLLASALa-PGGKFVLVGLLAGpt--atVTVTTDHLFTRQ-LSFLPFRGSEHlkrlgNARA 334 Chaetomium glob...
XP_001230010 267 ----grvVLGLDAVFGAAGEALAGLLs-RGATYVNYGSLGGvd--gvVRVSQKMVFWNE-VRFRNFRLSEQlgkrsVMEQ 338 Chaetomium glob...
XP_001463016 221 ----rgaALYLNGVGGRYFDSLLKCVg-PMAHVVTYGAQNGf----gLFISGSSLIYNE-VTMAGLFAPTFlssmsYGER 290 Leishmania infa...
YP_001260825 201 -------RAALDCVAGDATGRMAACLd-AEGTVLVFGHLSGe----pSTIRSQLLTGRG-LTVRGFSLRPAearmtPAAR 267 Sphingomonas wi...
XP_001699870 560 lhfharpRLALDAVGGDSAARLADALg-EGGELVVYGCMSGr----sPALTWQTLLFRD-LRVRGFNLRRWaa-agVPPP 632 Chlamydomonas r...
CAP95067     225 ------iVLAIDSVSDDSLARNMAASltAGGTLVTAGFLGTae---sQEGNLRQFLWQRnITLKSFRLSDClgrrtPPQQ 295 Penicillium chr...
EEH08365     212 ------iMLGLDSVFGQSGEKIASCLs-AHATFVNYGQLSGggpaasVNLTHQQVYWNR-LTFRCFRGTEQvalrtDSEI 283 Ajellomyces cap...
Feature 1                                                                            #     
1GYR_A       313 TSTLNQIIAWYEegk----ltDAKSIETLydgt-----kplhELYQDGVANs-------------KDGKQLITY 364 Candida tropicalis
1N9G_A       335 TSTLNQIIAWYEegk----ltDAKSIETLydgt-----kplhELYQDGVANs-------------KDGKQLITY 386 Candida tropicalis
XP_001212124 295 IQLLAWFGELFEq--------GQLVAPPVnkiewk-gdgaleERVRQALSQisggs-----agvvGNLKPVFQF 354 Aspergillus terreus N...
XP_001226017 335 EELIYETANMFId--------GTLKRPTVnvvdw---ttageGEVEEALKRai----------qlASGKEVGHI 387 Chaetomium globosum C...
XP_001230010 339 EDLLGWLADLIAr--------GELRAPVVerip-----vpmdGSFVEAFQEkvkgvlaaaaekkiGHRKHVLDF 399 Chaetomium globosum C...
XP_001463016 291 QAKLEFVLRAVQe--------AGIAYPMAtapsl----eklpEVWDDVYVN--------------GGRKAVVKM 338 Leishmania infantum J...
YP_001260825 268 DAMVAGLWAAAGq--------GAVELPIRavl--------plAEAERAIALar----------tpGRGRVLIDL 315 Sphingomonas wittichi...
XP_001699870 633 LGTSAAAATAAAstssgsssvGGGSATAAgyg--------gpGRYCAALEAlgk---------lvAAGMLTVAY 689 Chlamydomonas reinhar...
CAP95067     296 VALFEWFAELLAr--------GTLKAPALeyvtwkhgaerleKSLRDSIQRahd--------gaiGGRKTVLVF 353 Penicillium chrysogen...
EEH08365     284 KDLYAWFTELFAd--------GRLKSPKLnivnwsgerdilaTNIRAAIERqqs--------pvlGTEKTVFLY 341 Ajellomyces capsulatu...

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