1F8F


Conserved Protein Domain Family
benzyl_alcohol_DH

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cd08278: benzyl_alcohol_DH 
Click on image for an interactive view with Cn3D
Benzyl alcohol dehydrogenase
Benzyl alcohol dehydrogenase is similar to liver alcohol dehydrogenase, but has some amino acid substitutions near the active site, which may determine the enzyme's specificity of oxidizing aromatic substrates. Also known as aryl-alcohol dehydrogenases, they catalyze the conversion of an aromatic alcohol + NAD+ to an aromatic aldehyde + NADH + H+. NAD(P)(H)-dependent oxidoreductases are the major enzymes in the interconversion of alcohols and aldehydes, or ketones. Alcohol dehydrogenase in the liver converts ethanol and NAD+ to acetaldehyde and NADH, while in yeast and some other microorganisms ADH catalyzes the conversion acetaldehyde to ethanol in alcoholic fermentation. ADH is a member of the medium chain alcohol dehydrogenase family (MDR), which has a NAD(P)(H)-binding domain in a Rossmann fold of a beta-alpha form. The NAD(H)-binding region is comprised of 2 structurally similar halves, each of which contacts a mononucleotide. A GxGxxG motif after the first mononucleotide contact half allows the close contact of the coenzyme with the ADH backbone. The N-terminal catalytic domain has a distant homology to GroES. These proteins typically form dimers (typically higher plants, mammals) or tetramers (yeast, bacteria), and have 2 tightly bound zinc atoms per subunit, a catalytic zinc at the active site and a structural zinc in a lobe of the catalytic domain. NAD(H) binding occurs in the cleft between the catalytic and coenzyme-binding domains at the active site, and coenzyme binding induces a conformational closing of this cleft. Coenzyme binding typically precedes and contributes to substrate binding. In human ADH catalysis, the zinc ion helps coordinate the alcohol, followed by deprotonation of a histidine, the ribose of NAD, a serine, then the alcohol, which allows the transfer of a hydride to NAD+, creating NADH and a zinc-bound aldehyde or ketone. In yeast and some bacteria, the active site zinc binds an aldehyde, polarizing it, and leading to the reverse reaction.
Statistics
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PSSM-Id: 176239
View PSSM: cd08278
Aligned: 77 rows
Threshold Bit Score: 402.648
Threshold Setting Gi: 71021221
Created: 30-Apr-2009
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 21 residues -Click on image for an interactive view with Cn3D
Feature 1:NAD binding site [chemical binding site]
Evidence:
  • Structure:1F8F: Acinetobacter calcoaceticus benzyl alcohol dehydrogenase binds NAD, contacts at 4A
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                 ##                                     
1F8F_A         5 KDIIAAVTPCkGADFELQALKIRQPQg-dEVLVKVVATGMCHTDLIVRDqkyp--vplPAVLGHEGSGIIEAIGPNVTe- 80  Acinetobacter c...
EEU43772       7 IATEALVTEKpGDKFEMKPIILDEVRg-dEVLVEMKYSGICHTDVLLQNglip-gveyPAIFGHEGAGIIRALGSDIKdk 84  Nectria haemato...
EED16210      10 IETRALVVEKpQGPFVLHDVIFDEVRd-dEVLVEIKYSGLCHTDLVAQQglip-lagfPVILGHEGAGIVRRLGGCLKdq 87  Talaromyces sti...
EAA62546       3 IPTKAYLVEEeRGPFVLKDVVLDQLEp-dEVLVEMKYTGLCHTDLVVQAgilp-vgsfPAVLGHEGCGVIRQVGSKVSnk 80  Aspergillus nid...
XP_001228831   5 LQTEALVVDTvGGGFVMTPITIHDLRs-dEILVEMKYSGICHTDFYLQQggfgpltqfPAIAGHEGAGIIRGIGSNVKnk 83  Chaetomium glob...
EEP77961       7 ITTKAYVVESqGAPFTLRDVILDALQp-dEVLVELKYTGICHTDIVVQHggmp-igsyPAILGHEGVGIVRQIGSQVKdk 84  Uncinocarpus re...
EED20608      13 IDTLAYVVESkDAPFVLRTVVLDDLQp-sEVLVEIKYTGLCHTDVVVQEggmp-ighfPAVLGHEGIGIVKQVGTDVKdk 90  Talaromyces sti...
CAP97645       7 IPTTAFVVEKpGAPFVLQNVILDEVRa-nEVLVEMKYTGLCHTDIVVQQgaip-vgdyPAVLGHEGAGIVRRIGSAVKdk 84  Penicillium chr...
XP_001555351   1 MRTKALVVNSpGGEFVYRDVEVNDDIrdtEVLVQIVATGICHTDINFSQhdid--gmfPAILGHEGAGIISKAGSKVTn- 77  Botryotinia fuc...
XP_001904322   9 IETTALLVPSpGSPFTISPVLIQNLRp-nELLVEIKYSGLCHTDFLLRDgalp-kvtyPSIPGHEGTGTILAIGPAVKnr 86  Podospora anser...
Feature 1                                                                                        
1F8F_A        81 -LQVGDHVVLSYg--yCGKCTQCNTGNPAYCseffgrn--fsgadsEGNHALCTHd-----qGVVNDHFFAQSSFATYAL 150 Acinetobacter c...
EEU43772      85 sLQVGDHVLLSFn--vCGKCKKCVAGKPSFChihspvn-vgsvrlsDGSTPARLEd-----gRSVRSQFFGHSSFARVSV 156 Nectria haemato...
EED16210      88 sLKVGDHVLLSFs--sCMNCSYCAQGQNGLCpqmhain-ftgtrlaDGSNPASLRn-----gTSIRSKFFGQSSFSKLAV 159 Talaromyces sti...
EAA62546      81 aLKEGDQVFLSFr--tCRECTPCLAGHCGACihsqelsflrtrldkTKPSPISLPd-----gTPVHGQFFGQSSLSRLAI 153 Aspergillus nid...
XP_001228831  84 sLQVGDSVLLSFa--aCGECSHCQEDQFSRCtqfipln-ltgiirsDGSTPAKLSd-----gRAVRSQFFGQSSFSRFSA 155 Chaetomium glob...
EEP77961      85 sLSEGDAVLLGFr--tCRQCSPCLEGRCGACphmteyn-fvsarrrEGAKPIYSFpd----gTPVHGQFFGQSSLSKLAI 157 Uncinocarpus re...
EED20608      91 nLKPGDTVILSFr--sCGECKACLNDRHGSCprvtels-flstaraDGSSPISLPd-----gTPVHGQFFGQSSLSKMAV 162 Talaromyces sti...
CAP97645      85 sLQEGDLVFLSFsschEESCSPCSKGRNGFCdqmtsinfagargltAAESPISFPg-----gSPIRGQFFGQSSMSKLAV 159 Penicillium chr...
XP_001555351  78 -LEVGDCVILSYt--sCGECKYCVRKQTSFCgdweldn-fgigrgeDGSKAYEIPseeeeggSEITSHFFGQSSFSNYAV 153 Botryotinia fuc...
XP_001904322  87 sLSIGDDVLLSFt--sCNECHHCLEQSPSTKcklmppl-nlscvrdDGTTAYQMQd-----gTPVSGHFFGQSSFAKIAV 158 Podospora anser...
Feature 1                                   #   #                     ####                     ##
1F8F_A       151 SRENNTVKVTKd-----vPIELLGPLGCGIQTGAGACINALKVtpASSFVTWGAGAVGLSALLAAKVCGAs--IIIAVDI 223 Acinetobacter c...
EEU43772     157 VAERSVVKCPYp-----eSLPYYAPLGCGFQTGAGTVLNALKPdkTQTVVIFGMGSVGIAALMAAAYLQVk--QLVAVDV 229 Nectria haemato...
EED16210     160 VSENSIVKCELsd----eEFAIMAPMGCGYLTGAGTVMTVLKPrkQSTLAILGMGAVGLSALIAAKAMGVq--RLIAVDI 233 Talaromyces sti...
EAA62546     154 VSERSVVKCDVefd--seELGPLAPMGCGYLTGAGTVVNVLKPssASTMLITGMGAVGVAALLAARALGLt--RIVAVDI 229 Aspergillus nid...
XP_001228831 156 VHENCLVKCLYp-----qDMAIYSPMGCGYQTGAGTILNIFKPkpGQTVAVFGAGSVGFAAIMAAATIPVk--QIIAIDI 228 Chaetomium glob...
EEP77961     158 VAQDSVVKCEVdd----kSLQYLAPLGCGYSTGAGTVFNALRPkpESTIAILGMGAVGLAALLAAKSMGVg--QIIAVDI 231 Uncinocarpus re...
EED20608     163 VTEKSVVKVPAets--paDLQYLAPFSCGYLTGAGTVLNEFRPdaDSTIAIVGMGPVGFAALLTAKAIGVk--NLVALDI 238 Talaromyces sti...
CAP97645     160 VDERSVVKSPPgsgvtveDMAVLAPLGCGYLTGAGTVFNVLKPtsKSRLAVIGMGAVGVAAMLAARSSGVe--TVIAVDI 237 Penicillium chr...
XP_001555351 154 GNARCCIRVGGpgs--dvDLSSLAPLGVGIMTGAGAILNVLKPssDDIVCIIGAGAVGLAAIMALNLLEKkpkQVIAIDM 231 Botryotinia fuc...
XP_001904322 159 VSELSVVPIKGmt---eeEMGIWAPMGCGYQTGAGTILDVFKPrkEDVVVIFGMGGVGFAALMAAALILEv-eTVIAVDL 234 Podospora anser...
Feature 1        #  #              #                              ##                     ###     
1F8F_A       224 VESRLELAKQLGATHVINSktq----dpVAAIKEit----dGGVNFALESTGSpEILKQGVDALGiLGKIAVVGApqlGT 295 Acinetobacter c...
EEU43772     230 VDDKLANAKEFGATHVVNSrtcg-eggiEAKIREit----dGGADFAIDCTGLiPVIESLFGCLApGGTATSVGVpppNA 304 Nectria haemato...
EED16210     234 LDTKLSLAKSLGATDIVNSnni---eslGKALREla----rNGVDHIIDTTGLsFMIEEGINALGhGGSFAIVGAprpGG 306 Talaromyces sti...
EAA62546     230 VDEKLQLALELGASHTINTlkgp-dsdlAATIRMyf----pGGADYIIDTTGVgKVLQATLRALAvNGTFALVGAmppDT 304 Aspergillus nid...
XP_001228831 229 VERKLALAKEIGATHTINSstvq--gsvVDELKKlt---ggQGVDFAVDTTGVsSVVEKMLDCLAySGTAASVGApprEE 303 Chaetomium glob...
EEP77961     232 VDSKLELALSMGATHTINTkqv---pdlCSGIRAlf----pDGVDQIIDTTGVsALMQASMKALGhEGVLAVVGVprpGD 304 Uncinocarpus re...
EED20608     239 VDSKLEIAAQLTGARTINNssgsstnasGESLSEkilatypKGIDYILDTSGRaPLLQDSVKALAhGGTLALVGVpppTA 318 Talaromyces sti...
CAP97645     238 VDAKLELAKSLGASHTLNTk-------sVSDLTQglldilpDGVDCILDTTGVvPLLEAAVKALGhEGTLAIVGVaraGS 310 Penicillium chr...
XP_001555351 232 LDERLKLAKTYGATHVIKPtke---rslQSTLKRmt---nfEGIDISIDTTGKpEVVAELLEATAkLGTVCSLGVgklDA 305 Botryotinia fuc...
XP_001904322 235 VQEKLDLAKEMGARWVINGke------tLDVVGEmmkitggKGADFAVDTTGVgKVVQDMVQCLGqGGTAASVGAp--AP 306 Podospora anser...
Feature 1                          ###                                                      
1F8F_A       296 TAQFDVNdlllgGKTILGVVEGSGSPk-KFIPELVRLYQQGKFPFDQLVKFYAFDeINQAAIDSRKGiTLKPIIK 369 Acinetobacter calcoa...
EEU43772     305 VVRIEPQtflleNKRYVGVVEGGSNPq-KFIPELMELHRQGHFPVEKLCTVYSIKdFEKALADMRGGkVVKPIIH 378 Nectria haematococca...
EED16210     307 TITIDPLdmlrsCKRIVGVVEGASNPa-TLIPELIRLHREGLFPVERLSRTYSVTdLDIAIEDLKAGnIVKPVLS 380 Talaromyces stipitat...
EAA62546     305 ELKVNALdvltgCKKIIGVILAWSDPq-QIIPQLVQWYKEGKFPVHKIVRIYPATeLDKALEDLRAGqVIKPVLA 378 Aspergillus nidulans...
XP_001228831 304 KITVDVGlffglKKTWISVSEGDSHPp-EFVPKLIDLHRQGKFPIEKISKVYPVAdFEKAVSDMKDGtVIKPIIK 377 Chaetomium globosum ...
EEP77961     305 SIQIDALdllvsCKRVIGVIEGFADSk-EIIPQLVKLYRQGNFPVDRISTVYPAErLDQAIEDLKTGkVIKPVLS 378 Uncinocarpus reesii ...
EED20608     319 TIEVNALnlltsCKRIIGVIEGSANPk-KVIPHLIELFNQGKFPVQQLTKIYPANrLDEAIEDVKRGkVVKVVLS 392 Talaromyces stipitat...
CAP97645     311 SLNIDPLklmmgCQRVVGVIEGCANPa-VIVPQLIDLYKRGKFPVDKLAKIYAPSdFEQAMADLHSGsVIKPVIQ 384 Penicillium chrysoge...
XP_001555351 306 DVSINMFnavnsGRKYVGCCMGNSYPp-EFLPKLIQGWKDGKFPFTDLITKYDAKdMEKAKADILSGeAVKAVLI 379 Botryotinia fuckelia...
XP_001904322 307 GVKIEVDv----GMFFALKEEADTGRgwQFIPRLIQAYKEGKFPVDKISKVYPVEdFEKAIADTESG-KVSVVML 376 Podospora anserina D...

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