Conserved Protein Domain Family
MDR3

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cd08275: MDR3 
Medium chain dehydrogenases/reductase (MDR)/zinc-dependent alcohol dehydrogenase-like family
This group is a member of the medium chain dehydrogenases/reductase (MDR)/zinc-dependent alcohol dehydrogenase-like family, but lacks the zinc-binding sites of the zinc-dependent alcohol dehydrogenases. The medium chain dehydrogenases/reductase (MDR)/zinc-dependent alcohol dehydrogenase-like family, which contains the zinc-dependent alcohol dehydrogenase (ADH-Zn) and related proteins, is a diverse group of proteins related to the first identified member, class I mammalian ADH. MDRs display a broad range of activities and are distinguished from the smaller short chain dehydrogenases (~ 250 amino acids vs. the ~ 350 amino acids of the MDR). The MDR proteins have 2 domains: a C-terminal NAD(P)-binding Rossmann fold domain of a beta-alpha form and an N-terminal catalytic domain with distant homology to GroES. The MDR group contains a host of activities, including the founding alcohol dehydrogenase (ADH), quinone reductase, sorbitol dehydrogenase, formaldehyde dehydrogenase, butanediol DH, ketose reductase, cinnamyl reductase, and numerous others. The zinc-dependent alcohol dehydrogenases (ADHs) catalyze the NAD(P)(H)-dependent interconversion of alcohols to aldehydes or ketones. Active site zinc has a catalytic role, while structural zinc aids in stability. ADH-like proteins typically form dimers (typically higher plants, mammals) or tetramers (yeast, bacteria), and generally have 2 tightly bound zinc atoms per subunit. The active site zinc is coordinated by a histidine, two cysteines, and a water molecule. The second zinc seems to play a structural role, affects subunit interactions, and is typically coordinated by 4 cysteines.
Statistics
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PSSM-Id: 176236
View PSSM: cd08275
Aligned: 31 rows
Threshold Bit Score: 383.089
Threshold Setting Gi: 224010309
Created: 30-Apr-2009
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
putative NAD(P)
Feature 1:putative NAD(P) binding site [chemical binding site]
Evidence:
  • Comment:MDR family binds NAD(P) as a cofactor
  • Comment:defined by comparison to MDR alcohol dehydrogenase like family

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                      ###  #            
AAH73628      36 KSLLLSAHGGYDkvKLQVkk------------------gsPVPKA-----GEVLVRVKACGLNFADLMARQGvydr---l 89  African clawed ...
YP_001840528   4 EVYRIHKTGSID--HLQQkke-----------------mlRPPEN-----DEVTVEVRAIGLNFADVFSVYGlysa---t 56  Leptospira bifl...
ACO66369       7 TIWRVNKTGALS--GLERvdg----------------delAPPGP-----GEIRVKVKAIGLNFADVFSVLGlyga---t 60  Micromonas sp. ...
CAL54407      20 RAWEISSKGSLK--NLELn---------------------TVPSVvl-npDECRVRVRAIGLNFADVFTALGlysa---a 72  Ostreococcus tauri
XP_002294112  67 QCISIGRPGGLE--QLRLvtlregictigynvrhvssppyTSPNAtl-pdDCVTIRNEAFSVNYADCTIRWGlyesakqf 143 Thalassiosira p...
XP_002167077  14 VYTTLPECGYTK--GVNI----------------------EQQEIsd--sDCVVVETYACGVNYADIIIRWGlyksatdf 67  green hydra
YP_628504      4 RKVVIRKAGGYE--QLHLeev-----------------nqDSPGI-----GEVRVATEAIGVNYADCVIRMGlyasakey 59  Myxococcus xant...
YP_001615665   2 RKIVVHEPGSHD--KLRVe---------------------EHPDPsp-arGEVLLDVRAAGVNYADCVVRMGlyesakky 57  Sorangium cellu...
YP_692537     23 TAWETPKAGSLA--RLTQk---------------------TMPLPtl-plGQLRIGVHAVGLNFADIFALTGlysa---t 75  Alcanivorax bor...
NP_216428      2 RAVVITKHGDPS--VLQVr---------------------QRPDPpppgpGQLRVAVRAAGVNFADHLARVGlypd---a 55  Mycobacterium t...
Feature 1                                                                                     #  
AAH73628      90 psLPVSLGMECAGIVEeLGEGVTDr---------qVGDKVMVLNrFGLWQELVTIQTnhTFLMPDg-MSFEEGAAFLVNY 159 African clawed ...
YP_001840528  57 pkGSFIPGLEFSGKIVkIGPNVKDf---------eVGDQVFGVTrFGAYATHLNISEktIFTLPKd-WTMQDGASFAVQA 126 Leptospira bifl...
ACO66369      61 pdCPFVPGLECCGIVEaIGESDPSlpegavvptvaVGDSVMVVTrFGAYASAVNAPLhqCRPLPSg-WTHEQGAAFLVQG 139 Micromonas sp. ...
CAL54407      73 pkGRFVPGLECSGEIVeLPNGYAGdf--------hVGDKVMCVVrFGAFADEVAVPMtqMRPLPNg-WTFEEGAGFLVQG 143 Ostreococcus tauri
XP_002294112 144 vgWPIVPGFDVAGIVEsVGESNNSnndn----dlkVGDRVFGCTlFGAYSNRVIVPKiqLRRIPDgiESFAQAAAFPAVS 219 Thalassiosira p...
XP_002167077  68 vgWPITPGFEFSGTIKsVGQNVKDf---------iVGDKVAGLTcFGAYTTCIKAPAymIRKVPSn-VSMSTAAGFLTVA 137 green hydra
YP_628504     60 vgWPITPGFEFAGHVDaVGPGVEDl---------aPGARVFGVTrFGGYATHVTVPRhqVFTLPAr-LSMEQAAGFPTVF 129 Myxococcus xant...
YP_001615665  58 vgWPITPGFEFSGTVAaLGEGVTDl---------aLGAPVFGVTrFGGYASRVAVPRhqVFPLPAr-LTPEEAAGFPAVF 127 Sorangium cellu...
YP_692537     76 pqGAFTPGLEFSGTVIdAGAHNKAtds-----lfkVGDRVMGVTrFGGYCSVIDSEPdyLMPLPHh-WDFPQGAAFPAQT 149 Alcanivorax bor...
NP_216428     56 pkLPAVVGYEVAGTVEaVGDGVDPn---------rVGERVLAGTrFGGYCEIVNVAAtdSVVLPDa-LSFEQGAAVPVNY 125 Mycobacterium t...
Feature 1         #                                 #### ##                     ##  #            
AAH73628     160 ITAYMILFhFGNLRPN-------------QSVLIHMAAGGVGTAAVQLCKTv---dNITIFGTASasKHENLKeng---- 219 African clawed ...
YP_001840528 127 LTAYYALIpLGQVKEG-------------DHVLVHSAAGGVGIMAGHIIQKkk-aiAIGLVGDSV--KFSILKe------ 184 Leptospira bifl...
ACO66369     140 LTAFYALRaLGDVKKG-------------QTVLVHSAAGGCGLFGLGICNAlg-atPIATVGSQT--KVTAVLerfpdmd 203 Micromonas sp. ...
CAL54407     144 LTSYYGLRaLGDVKRG-------------STVLVHSAAGGCGLMSLGICEAvg-aePIAVIGNAG--KEETLKerfps-- 205 Ostreococcus tauri
XP_002294112 220 LTALYALH-LAGYFPIfsseggsrikfqnKAILVHSAAGGVGSFLVQMSKIlglgpVVGVVGSSS--KVEEATalg---- 292 Thalassiosira p...
XP_002167077 138 ATAYYAAFeLCKLRVT-------------DSVLVHSAAGGVGSMLCQMLRNag-cnVVGVVGATH--KVEAAKnlg---- 197 green hydra
YP_628504    130 LTAYYALFeLAHPRPG-------------ANVLVHSAAGGVGGALLQLGRIag-crMVGVVGGTH--KVETARaqg---- 189 Myxococcus xant...
YP_001615665 128 MTAYYALFeLVRLRPA-------------MSLLVHSAAGGVGSALVQLGKIag-crVVGVVGAAH--KVEAARrlg---- 187 Sorangium cellu...
YP_692537    150 LTAWYALTtLGNLQPD-------------HNVLIHSAAGGVGLQAMKLTQAlg-atPVGTVGNPN--KARILQdmg---- 209 Alcanivorax bor...
NP_216428    126 ATAWAALHgYGSLRAG-------------ERVLIHAAAGGVGIAAVQFAKAa----KAEVHGTASpqKHQKLAe------ 182 Mycobacterium t...
Feature 1                #                               ## #                  ##                
AAH73628     220 --vlHPIDYrs---nDYVEEVRKVs------PKGVDIVMDPLGGtdTCKAFNLLKPMGTLVIYGAANLl---------tG 279 African clawed ...
YP_001840528 185 ----VGYDFfltrspNFKEEMHKIl-----kGAPLKIVLECLGGryFHDSYDLLAPMGRLVTYGSANFtp--------sH 247 Leptospira bifl...
ACO66369     204 pdtiIVRDRr-----RFREQVAAAaaaagseRGTCDVVMDAVMGdfFEGGWANLARGGRYVVYGAADLtpag---dlawF 275 Micromonas sp. ...
CAL54407     206 lravIVRDRk-----RFKQQVKEAcds-igaSEGCDIVFDATLGdfFQGGWDNLARGGRYVVYGAADLtpr-----gdsI 274 Ostreococcus tauri
XP_002294112 293 --cdVVIDKst---sDLWTEAEKAs------PTGYATIMDANGVstLQQSYDHLAPSGRLVVFGFHSNlpmg----samL 357 Thalassiosira p...
XP_002167077 198 --clSVIDKsv---hNLWEEAKKIa------PNGYAAIFDANGVetLKDSYNHVKPGGRLISYGSATLfprstktsngeL 266 green hydra
YP_628504    190 --aeVVIDKsr---eDLWSVAKKAa------PRGYDVVLDANGPstLRDSYKHLASPGKLVIYGFHSMlp-------rtG 251 Myxococcus xant...
YP_001615665 188 --adAVIDKss---ePLWAAAERLa------PEGYDVILDANGVetLRQSYRHVASAGKLVVYGFHTMlp-------rqG 249 Sorangium cellu...
YP_692537    210 --fsDVLVRq----rDFSRQLTES-------GHRFDLVLDAIGGtvQQASFAALNPMGRLVVFGAAEFtp--------tG 268 Alcanivorax bor...
NP_216428    183 ----FGVDRa----iDYRRDGWWQg------LGPYDVVLDALGGtsLRRSYTLLRPGGRLVGYGISNMq---------hG 239 Mycobacterium t...
Feature 1                                         ###                                            
AAH73628     280 QKKNIMALAKSWWnqfsvta-mqlisaNKSVCGFHLghLDEELELItsvvstLLDLYKQGK-IKPKIDTMFSFEQVgDAM 357 African clawed ...
YP_001840528 248 SFRNWFSIVYSYLtrpkidp-lsmisdNKSVLGFNLiwLWNEIDELrk---hFSELMMLSL-PKQTIGHQFSFDSMhDAL 322 Leptospira bifl...
ACO66369     276 NVLGWVKLGWKYLwrprvdp-tnlpgeNKSVMGFNLiwMFDKIVELg----gLLTDLDALRlPAPKVGESFDFDRLpDAI 350 Micromonas sp. ...
CAL54407     275 GVVGWVKLAWKFLrrkkvdp-lmlpgeNKGILGFNLiwQFDKQEELa----rLLAELNALNlPAPKVGATFNFTDLpEAM 349 Ostreococcus tauri
XP_002294112 358 SPLEWIRMTKKVAimpkfda-mdltvsNKSILGFNLsfFAEERDILsdmfdqVVEWIEEKK-LLCPRVVEMEMKNVgEAH 435 Thalassiosira p...
XP_002167077 267 STYNWLKLVWNYKnrtvfdp-memtssNKSVMAFNLsfMFGEKELIkecldiLFKWISEGS-IKLDNITEYSLRDVaNAH 344 green hydra
YP_628504    252 GRPNYAKLAWDWLrtprfdp-ltltndNTSVLAFNLsyLFEQRAVLeesmaqLLGWLEEGK-LVPPPVKSFPLDAVaDAH 329 Myxococcus xant...
YP_001615665 250 GRPSYPKLAVDFLrmprfnp-ldlcndNKSVLAFNLsyLFHRTEILreamdrLIGWVEEGR-LSAPPVKTFPLERAgEAH 327 Sorangium cellu...
YP_692537    269 NRPNYLKAAWRYLrrprydv-mdmisnNRSVMAFNLiwLWEQKTIMq----tLLRELQAIDlPPPHVGHRFAFDDAhAAL 343 Alcanivorax bor...
NP_216428    240 EKRSMRRVAPHALsmlrgfnlmkqleeSKTVIGLNMlrLWDDRRTLepwiapLTKALNDGT-ILPIVHAIVPFAEApEAH 318 Mycobacterium t...
Feature 1                        
AAH73628     358 RQMQEKKNVGKVILIP 373 African clawed frog
YP_001840528 323 RTMQSGHTIGKIVINV 338 Leptospira biflexa serovar Patoc strain 'Patoc 1 (Paris)'
ACO66369     351 RTFQSGGTSGKVVVTL 366 Micromonas sp. RCC299
CAL54407     350 RLFQTGQTTGKVVVTV 365 Ostreococcus tauri
XP_002294112 436 TLIQSGKSIGKIVLTT 451 Thalassiosira pseudonana CCMP1335
XP_002167077 345 RDLESGNSVGKIVLLF 360 green hydra
YP_628504    330 RALESGGTVGKLVLVP 345 Myxococcus xanthus DK 1622
YP_001615665 328 RAIESGSTVGKLVLTL 343 Sorangium cellulosum 'So ce 56'
YP_692537    344 ALLQSGQSVGKVVLEV 359 Alcanivorax borkumensis SK2
NP_216428    319 RILAARENVDKVVLVP 334 Mycobacterium tuberculosis H37Rv

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