Conserved Protein Domain Family
MDR9

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cd08274: MDR9 
Medium chain dehydrogenases/reductase (MDR)/zinc-dependent alcohol dehydrogenase-like family
This group is a member of the medium chain dehydrogenases/reductase (MDR)/zinc-dependent alcohol dehydrogenase-like family, but lacks the zinc-binding sites of the zinc-dependent alcohol dehydrogenases. The medium chain dehydrogenases/reductase (MDR)/zinc-dependent alcohol dehydrogenase-like family, which contains the zinc-dependent alcohol dehydrogenase (ADH-Zn) and related proteins, is a diverse group of proteins related to the first identified member, class I mammalian ADH. MDRs display a broad range of activities and are distinguished from the smaller short chain dehydrogenases (~ 250 amino acids vs. the ~ 350 amino acids of the MDR). The MDR proteins have 2 domains: a C-terminal NAD(P)-binding Rossmann fold domain of a beta-alpha form and an N-terminal catalytic domain with distant homology to GroES. The MDR group contains a host of activities, including the founding alcohol dehydrogenase (ADH), quinone reductase, sorbitol dehydrogenase, formaldehyde dehydrogenase, butanediol DH, ketose reductase, cinnamyl reductase, and numerous others. The zinc-dependent alcohol dehydrogenases (ADHs) catalyze the NAD(P)(H)-dependent interconversion of alcohols to aldehydes or ketones. Active site zinc has a catalytic role, while structural zinc aids in stability. ADH-like proteins typically form dimers (typically higher plants, mammals) or tetramers (yeast, bacteria), and generally have 2 tightly bound zinc atoms per subunit. The active site zinc is coordinated by a histidine, two cysteines, and a water molecule. The second zinc seems to play a structural role, affects subunit interactions, and is typically coordinated by 4 cysteines.
Statistics
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PSSM-Id: 176235
View PSSM: cd08274
Aligned: 18 rows
Threshold Bit Score: 450.981
Threshold Setting Gi: 111027074
Created: 9-Feb-2009
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
putative NAD(P)
Feature 1:putative NAD(P) binding site [chemical binding site]
Evidence:
  • Comment:MDR family binds NAD(P) as a cofactor
  • Comment:defined by comparison to MDR alcohol dehydrogenase like family

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                    ###  #                              
YP_644336      1 MKAVLLTGHGGLEKLEYREd--iPTPSPa--pGEVLIKVGAAGVNNTDIWTREGAYGTEidseats-------------g 63  Rubrobacter xyl...
YP_555339      6 MKAVVLTAHGGLDKLVYRD----DVPVPvcraGEVLLDVTACGLNNTDVWVREGAYGTDddpqavst------------w 69  Burkholderia xe...
YP_742891      6 MHAVQLTRHGDLDALVYRDd--vPRPEPa--rGEVLIEVSACGMNNTDVWVRQGAYGTEtdpdsvst------------w 69  Alkalilimnicola...
NP_107504      1 MAAVLLTGHGGPEKLVYRTdvkvPSPDP----GEVLVEVSACGMNNTDVWVRQGAYGTEedaaavst------------w 64  Mesorhizobium l...
YP_914101      8 MCAMVLTGHGGTERLEWREd--wPVPRPg--pGEVLVRITACAKNNTDRKVREGRYSTAddgavts-------------f 70  Paracoccus deni...
YP_168595      7 MSGVYLTRHGGPEALAWRDd--iPVPAPg--pGQVLVRVQAAGVNNTDVNTRIGWYSSDvtgatdsget--------gea 74  Silicibacter po...
YP_709052     20 MRAVVITGHGGLDVIEEREv---PVPRPg--pGEVLVRVHAAGCNNTDLWTREGSYGAAedpdvr--------------- 79  Rhodococcus sp....
YP_943323     10 MTAIVTTGSGGYDQLVCKQv---PIPDLc--nGEVLVKVLAAGVNNTEINTRLGWYSSSvhgstndtsnsek--dqpkad 82  Psychromonas in...
YP_003133889   8 MAAVLLTGHGGLEKLEYRTd--vPIPRPa--aDEVLIQVGAAGVNNTDINTRIGWYSKGvtaetgaggaagfesvdaada 83  Saccharomonospo...
YP_003153923   1 MQAVQLIGHGGLEQLVHSPd--vPTPAPa--aGEVLIDVHACGMNNTDIWVREGAYGSDtdpgaist------------w 64  Brachybacterium...
Feature 1                                                                                        
YP_644336     64 wrrgkpMEFPRIQGMDIAGRIAEVGEGVPrsRIGERVLVDFVLRsgegde-glveadLVGSERDGGFAEYVAVPATNAVa 142 Rubrobacter xyl...
YP_555339     70 rrgrstLSFPRIQGADIVGRVIAVGHGVSdaRVGERVIVDASIYnrddds--ladidYIGHGRDGGYAEYTAVPAQSAHr 147 Burkholderia xe...
YP_742891     70 rrgrstLTFPRIQGTDIVGTVVAVGEGVPeaRIGERVMVDFSLYnradds--ladidYIGHGRDGGYAEYTAVPSENAHv 147 Alkalilimnicola...
NP_107504     65 rrqgntLVFPRIQGTDTVGTIVSVGEGVSpeRIGERVMVDFSIYnrddds--ladidYMGHGRDGGYAEYQTVPAENAHv 142 Mesorhizobium l...
YP_914101     71 amagntLSFPRIQGADVVGHVVAVGQGVPtdRIGERGLLDFNIYpdardd-lnlapdYFGHGADGGFAEYGCFPSEQFHa 149 Paracoccus deni...
YP_168595     75 ggwagaLSFPRIQGGDLCGRIVALGAGVTgwATGQRVTCQINLPrptpdn--pfgfvALGSELDGAFAEYCLLYADDLFd 152 Silicibacter po...
YP_709052     80 tgwlgpLDFPRIQGADVAGRVVASGDGAAagPIGARVLVDPAEYdgpgld--arpadVLGSERNGGFAEYVVVPSTRAHs 157 Rhodococcus sp....
YP_943323     83 ggwnkqTAFPFIQGTDCCGVVVNVADDVDtgLMDKRVLIRPCIRehgfg---sldniWMGSDFDGAFAEYVKIRATEVFp 159 Psychromonas in...
YP_003133889  84 awsgtaLRFPRIQGADVCGRIVDVGEGVPraRIGERVLVRTMLRapvedr--pfecwTFGSECDGGFAQFTVAPARDTYa 161 Saccharomonospo...
YP_003153923  65 rrgrstLTFPRIQGADIVGVVAAVGEGVPaeRLGERVMVDFSLYhrpegddsladidYIGHGRDGGYAEYVTVPAENAHp 144 Brachybacterium...
Feature 1                        #   #                   #### ##                  ## #           
YP_644336    143 --VESPLSDEELATFPTAYLTAEHMLNRAsvaeDETIFVTGASGGVGSALLQLARLRGArVIALVGAGKENKAYELGAe- 219 Rubrobacter xyl...
YP_555339    148 --IDSACSDAELATFWCAYHTGEQMIERAavksGETVLITGASGGVGSAIVQLCRARGAvPYAVVSKGKEEAVRELGAq- 224 Burkholderia xe...
YP_742891    148 --VDTDMSDAELATFCCAYLTGEHMLERArvqaGERVLVTGASGGVGSGIIQLCRARGAiPYAVTSRDKAEAVRGIGAe- 224 Alkalilimnicola...
NP_107504    143 --VDTDMSDVELATFCCAYLTGEQMLERAglkaGERVLVTGASGGVGSGIVQLARARGAiPYAVVGKGKEQAVLDIGAe- 219 Mesorhizobium l...
YP_914101    150 iaNPALGDAELATLGMCSWQTGYRMLTSAgvgaGEHVLVTGASGGVGTALLQLCRVIGAiPHAVASAGKAEALHALGAe- 228 Paracoccus deni...
YP_168595    153 -vTASPLSDIEIAAMPCAYGTAWNLLARCgvgpGDAVLITGASGGVGLAAVQLAAHLGAtVTAMTAPAKAEAVTAAGAt- 230 Silicibacter po...
YP_709052    158 -vDESPLTDVELAALPIAYGTALGMLDRGavseGHTVLVTGASGGVGLAAVQLAHSRGArVVAVCSSDKGEAVRSAGAda 236 Rhodococcus sp....
YP_943323    160 --VVCNWSDAELGTIPCAYGTSENMLNRAnlraGETVLVAGASGGVGSATVQLAKRRGAkVIAIAGEEKHDKVAPLGAd- 236 Psychromonas in...
YP_003133889 162 --VRSDRSDVELAALPCSYSTAENMLRRAfv-gAERVLVTGASGGVGLAAVQLAKRRGAtVVAVCSAAKAAEVRAQGAd- 237 Saccharomonospo...
YP_003153923 145 --VTSELPDAALATFCCAYLTAEHMLDRArvttGERVLVTGASGGVGSALLQLARVRGAiPYAVTSPGKEAALLAIGAe- 221 Brachybacterium...
Feature 1            #                        ## #                         ##                    
YP_644336    220 SVVTRGTpdlpaa-vakaaDGHPVDVVADVVGGEvFLDLLRILR-------PLGRYVTAGAIAGPLVRLDLRTVYLKHLQ 291 Rubrobacter xyl...
YP_555339    225 GVMLRDSedfaaa-vermtGGRPVDVVADLVGGAlFGKLLNVLR-------PEGRYTTAGAIAGPVVQLDLRTMYLKQLQ 296 Burkholderia xe...
YP_742891    225 AVIPRESgdlvta-vdqatEGRPIDVVADLVAGPlFNDLLRVLR-------PEGRYTTAGAIAGPVVQLDLRTLYLKHLQ 296 Alkalilimnicola...
NP_107504    220 AVITRGVadlpra-vaevtGGQPIDVVADLVGGAiFNDLLRILR-------PEGRYTTAGAIAGPVVQLDLRTMYLKQLQ 291 Mesorhizobium l...
YP_914101    229 TVIDRNAvgdwpsaidratGGATIDAVMDLVGGEmTVPLIQAMCrdmrrrtTPPRLSIAGASAGNVTALPWTLIYLNQVR 308 Paracoccus deni...
YP_168595    231 TILGRDDt----------pPPAQFDRVIDVVGGPlWPDLIRALK-------PGGHYAVSGAIAGPMVSADLRQIYLPDIT 293 Silicibacter po...
YP_709052    237 VVDRRGGqvlad---aagaAPEGYDAVVDVVAGAvLGPGLSLLR-------TGGRWVVAGALGGWSVDIDVRKLYLANLS 306 Rhodococcus sp....
YP_943323    237 ILLGRSAnll------velGEQSVDVVVDNVGGSgFPTMLKLLK-------RGGRFVSSGAIAGPIVELDMRDMYLKDIT 303 Psychromonas in...
YP_003133889 238 RTVGRGAdlv------galGASSVDVVIDLVGGSqCPELLGLLR-------PGGRYAIAGAIAGPIAEIDLRTLYLKDLS 304 Saccharomonospo...
YP_003153923 222 STILRDApdlvg--etartVDGPVDVVADLVAGPlFGDLLRILR-------PEGRYITAGAIGGPVVELDLRTVYLKHLE 292 Brachybacterium...
Feature 1           ###                                                     
YP_644336    292 LIGSTVGTRAEFIELVRYIESGKIKPLLANTYPLSEIRrAQEDFLR--KDFFGKLVIIP 348 Rubrobacter xylanophilus DSM 9941
YP_555339    297 LNGSSQGTRGAFKRVLDYIQAGTIRPVLAATYPLSKFHeAQTQFMA--KRFVGNIVVVP 353 Burkholderia xenovorans LB400
YP_742891    297 LHGSSQGTRGDFRRLVGYIERGQVRALLYNTYRLSDFHrAQRDFME--KSYIGKLVVVP 353 Alkalilimnicola ehrlichei MLHE-1
NP_107504    292 LHGSSQGTRADFRRIVGYIEAKKIRPLVGGVYRLSDFHrAQADFVA--KDFVGKLVVVP 348 Mesorhizobium loti MAFF303099
YP_914101    309 IFGVSHGTRAEARQLIGWIGENRLKPVLAGIMPLSRLReAERLFVErgRDFIGKLVIVP 367 Paracoccus denitrificans PD1222
YP_168595    294 IHGATYQPREIFADLIALINAGAIRPLVSATFPLDQIAqAQAEFMT--KRLPGKIVLIP 350 Silicibacter pomeroyi DSS-3
YP_709052    307 LVGSTMHTPRIFDHLVEIARRGEVRPVVAATFPLGKVReAQAQLAQ--RRHVGKLIVVP 363 Rhodococcus sp. RHA1
YP_943323    304 LIGTTAWEKTVFPDLVRYIEQGEIHPVLAKTFPLSSIAeAQKEFLE--KKHVGKFVLIP 360 Psychromonas ingrahamii 37
YP_003133889 305 VFGCTCQGDEVFENLIGYIEREEIRPVVSRTYPLREIAqAQRDFLA--KKYPGKLVLVP 361 Saccharomonospora viridis DSM 43017
YP_003153923 293 LHGSSQGTRTAFRRLLGHIESGAIRPLLDRTFRLSELHdAQRAFLT--RTHIGKLVVVP 349 Brachybacterium faecium DSM 4810

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