Conserved Protein Domain Family
MDR8

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cd08273: MDR8 
Medium chain dehydrogenases/reductase (MDR)/zinc-dependent alcohol dehydrogenase-like family
This group is a member of the medium chain dehydrogenases/reductase (MDR)/zinc-dependent alcohol dehydrogenase-like family, but lacks the zinc-binding sites of the zinc-dependent alcohol dehydrogenases. The medium chain dehydrogenases/reductase (MDR)/zinc-dependent alcohol dehydrogenase-like family, which contains the zinc-dependent alcohol dehydrogenase (ADH-Zn) and related proteins, is a diverse group of proteins related to the first identified member, class I mammalian ADH. MDRs display a broad range of activities and are distinguished from the smaller short chain dehydrogenases (~ 250 amino acids vs. the ~ 350 amino acids of the MDR). The MDR proteins have 2 domains: a C-terminal NAD(P)-binding Rossmann fold domain of a beta-alpha form and an N-terminal catalytic domain with distant homology to GroES. The MDR group contains a host of activities, including the founding alcohol dehydrogenase (ADH), quinone reductase, sorbitol dehydrogenase, formaldehyde dehydrogenase, butanediol DH, ketose reductase, cinnamyl reductase, and numerous others. The zinc-dependent alcohol dehydrogenases (ADHs) catalyze the NAD(P)(H)-dependent interconversion of alcohols to aldehydes or ketones. Active site zinc has a catalytic role, while structural zinc aids in stability. ADH-like proteins typically form dimers (typically higher plants, mammals) or tetramers (yeast, bacteria), and generally have 2 tightly bound zinc atoms per subunit. The active site zinc is coordinated by a histidine, two cysteines, and a water molecule. The second zinc seems to play a structural role, affects subunit interactions, and is typically coordinated by 4 cysteines.
Statistics
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PSSM-Id: 176234
View PSSM: cd08273
Aligned: 21 rows
Threshold Bit Score: 317.668
Threshold Setting Gi: 224012172
Created: 23-Jun-2009
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
putative NAD(P)
Feature 1:putative NAD(P) binding site [chemical binding site]
Evidence:
  • Comment:MDR family binds NAD(P) as a cofactor
  • Comment:defined by comparison to MDR alcohol dehydrogenase like family

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                       ###  #                            
YP_001522013    3 YRHVIISRHGGPDVLEIvm---dERPEPRA----GEVGVRVLATGVAFTDVLIREGLYLGLPk---vPFTPGYEIVGIVD 72   Acaryochloris...
P34055          5 NRKVVITAYGPPSTALQfv--teDLPPPPK----DHVQVKILYAGFAGADVNMRLGVYPMQSa---pPFTPGYCFAGRVS 75   Trichoderma a...
XP_362976      12 IRKVIVTKFSPDPSESLaiv-taILPPPPA----SHVQVQVLYSGFSGADVAMARGLYPMQRs---aPLTPGYCMVGRVV 83   Magnaporthe g...
XP_001799173    5 IRKVIYSGAGGPEVVSVvs---aEIQAPAA----NEVQMKVLYAGMGGADVAMRVGCYPQQKsp--vGLTPGYSLVGRVH 75   Phaeosphaeria...
XP_002181849  110 VQEKVVYHHFGTDAMNLlnvetaDIPQPEAp---NHVVVKIHASTVSLDDCILRRGFCFDVTspislPVTPGMDFVGKVV 186  Phaeodactylum...
XP_383733       6 IRKVVYSAFGGPSNVSVit---aQISPPAK----YEVQCDVIYSGFSGADIQMRIGTYPMQRd---aPLTPGYCFVGRVS 75   Gibberella ze...
XP_002293662  284 QSVVVVTKHGNVDKLLLq----eLSIIPTAlt-pDQVVIKVECSTITLQDCMIRRGKWYDMQp---lPFIPGSDLVGTIY 355  Thalassiosira...
XP_002293115  380 HKDIMIHAPGGASDLVVr-----AMYYTPKvedgGDVVIEVEASTVSFRDCLLRRGIGMDKAp---fPVIPGCETVGTIF 451  Thalassiosira...
XP_002293064  708 QRELVWTYNGDGELTKRd----sEVKFEEMevgdGDVLVKVEASTITARDCQKTRHKQSDAQt----DFVPGFEVVGRIA 779  Thalassiosira...
EEU36065        5 FRKVVIPKYGDASVLEIve---aTLPQPAA----GEVQVVPIYSGFSGADINMRRGVYPSQQk---pPLTPGYCLVGTVK 74   Nectria haema...
Feature 1                                                                  #   #                  
YP_001522013   73 QLGSGV-----AGLDIGQRIAALTv-vGGYSEYVCLPAHECVlVPPEVDVAEAVGLVLQYVTAYQLLHRIAK-----VQS 141  Acaryochloris...
P34055         76 VNGPGS-----GKFEPGTLVTALTk-yDSDAEYINIPEKYLLaIPDGVDPKVAAALPVDWSTAYGMVHRAAK-----VSE 144  Trichoderma a...
XP_362976      84 SAGPRAcgrpnTDWEQGTLVTALTv-yDSEAERINIQEKYLIrVPEGIEPTVATALTLDWNTAYGMVEHAAR-----VRP 157  Magnaporthe g...
XP_001799173   76 QNGASC-----SKFNDGDLVACLTk-yDSEAELCNFPEKYLVpVPEGVDLRQAVAMVTDWTTAYGMAYRAAK-----ISK 144  Phaeosphaeria...
XP_002181849  187 ACGSQV-----DDFKNGEWVAGLVr-tGGNARFISVAQCSLVpVPKMLDSAEAVCMVSTYTAAYQTMRVVADrntvfSMQ 260  Phaeodactylum...
XP_383733      76 ANGPKS-----TKFYPGQLVGALTm-yDAEAQKINVAEKYLIsIPEHVEMRQALGVILDWNTAYCLVHRATDl----VRK 145  Gibberella ze...
XP_002293662  356 ELGKDAlr--rGGLSIGDRVAAVVp-sGGNAKYISLDYRQIIrVPQGTDPVTALCLSSTYVPAREALDCARKfn--tPLT 430  Thalassiosira...
XP_002293115  452 ALGKIAk---sRGYRTGDRVVAFNrhgGGNAKYAKFDVSCIAsIPESVDGIDAVCLVDVYMTAYQALRIAKKng--tPLT 526  Thalassiosira...
XP_002293064  780 QAGRNLkg--nQIYAVGKRVAALLpngGGNARYVVVDNKNVIpLPETARHEDIICLVSTYMTAYQSLKKARKdg--tSLT 855  Thalassiosira...
EEU36065       75 ANGSGS-----SKFKTGDTVACLTk-yDAQAQLVNLPEKHLIpVPEGSDLQQVTGLLLDWCTAYGMVHQVAK-----VKK 143  Nectria haema...
Feature 1                #### ##                 ##   #                 #                      ## 
YP_001522013  142 GDRILIHGAAGGVGTALLELGHLAGLKMYGTDS-TTKHDLIANLG----ATPIDYLPENFqpqirrltPNGMDVVFDAIG 216  Acaryochloris...
P34055        145 GQRVFIHGISGAVGQAVMYLSLLQGATVYGTAS-ERNHAALKEAG----AHPYLYTNKDWiaa--mkdLGGVHAVFDALG 217  Trichoderma a...
XP_362976     158 GQSVFVHGLSGAVGYALATLCLLRGATVFGTAS-ERNHATLAGLG----VTLFVYTNKDWida--vqtQGGVDAVFDPLG 230  Magnaporthe g...
XP_001799173  145 GTRVFIHGLSGSVGFGLLTFCKMQGAEVYGTAS-EKNHAKVRESG----ATPFLYSDKEWmva--mknMGGAHVVFDPLG 217  Phaeosphaeria...
XP_002181849  261 GKKVLIVGGMHNVGQALIELCTKAKAEIFATAP-DRRHSYIRNMLg---ATPLPESASEWst----ivDGEMDYVLDGVC 332  Phaeodactylum...
XP_383733     146 GQRVFIHSISGAVGYATMTLCLLEGAEVYGTAS-ERNHASLRELG----VTPFTYADKNWkde--mkqRGGAHVVYDPIG 218  Gibberella ze...
XP_002293662  431 GANILIIGGNGPSGLAMIELAMLEGANVYTTAD-ERHHDFLTQLG----AKCLPIDPKKWlp----iiQGKMDAVLDSVC 501  Thalassiosira...
XP_002293115  527 DSYVLITDGYSPVGQAAIELAKLEGAHVFVTTTeSRQDAYMQTLG----VKCLPLSPSKWlp----kiKGKMDVVIDNTC 598  Thalassiosira...
XP_002293064  856 DRKILITNASSPVGQAVLTFALEEGTSVFAASE-KRFEGYLRGLSkkklKWYPTANTKDWlp----alEGKMDIVIETNG 930  Thalassiosira...
EEU36065      144 GQTVFIHGISGSVGYATMKLVQLLGATAYGTAS-ERNHAAIREQG----GIPFVYTDKNWika--mkdTGGVDAVFDSIG 216  Nectria haema...
Feature 1         #                              ##                                               
YP_001522013  217 GRHLLGSYQTLAP------------GGQLISYGfssalsahwgrllkv-------------------gesmglltllsiw 265  Acaryochloris...
P34055        218 FESFDESYSILTPk----------eRSVVVAYGnnlsnltgakrrspwi------------------pmakllfknlyfw 269  Trichoderma a...
XP_362976     231 FQSWEESYSILATt----------rPSVLVGYGgnqhllpsssekeagdneavalrktllptqvpqivklvaknikrfig 300  Magnaporthe g...
XP_001799173  218 FESWDESWSILAPe-----------GGHLIGYGgntdilngrkprsqip------------------qvakliakglnpf 268  Phaeosphaeria...
XP_002181849  333 EDGLVPALQALKQ------------NGKLACFGhssilree----------------------------------mglfg 366  Phaeodactylum...
XP_383733     219 FEHYNDSWDILIRn----------ePSRLVGFGgnmniiqgddakprsqf----------------ssqvkllakngclv 272  Gibberella ze...
XP_002293662  502 LDGYESSSLALNR------------EGTLVCTGmsavytqgqmsvlglgd---------------vrnmnawyvtmksrc 554  Thalassiosira...
XP_002293115  599 FDSYDSSWKALSA------------KGVLVCTAmtsiycfndlgqncgcgaf-----------gdmreiearwatlkaky 655  Thalassiosira...
XP_002293064  931 IDDMSCHRALAPDgtmictsntsleGGEPKEFGfksllsq-----------------------------------lkvky 975  Thalassiosira...
EEU36065      217 FESFHESYDILNS------------HGIVVGFGankysltdeapvpatw------------------ptvkfllkgqipw 266  Nectria haema...
Feature 1                 ###                                                            
YP_001522013  266 pdRRSAVFYNItdlkrrqpnWFRDDLTSLldLLADGSIQPIIADRLPLadAAVAHNLLDHAav-rGQLVLL 335  Acaryochloris marina M...
P34055        270 snKGAIFYFITrdq----ktFEPELQLLLn-MTRDGIITPPIKAVWEFddIKEAHEAWCKGsg-mGSPVIR 334  Trichoderma atroviride
XP_362976     301 dnRRTVFYYIDrdh----kdFRPDLEAVMd-LCRTGKITVPIKTTWSLdqVRDAHRGWGRKeg-mGSMVIK 365  Magnaporthe grisea 70-15
XP_001799173  269 cpNKSTFFYIDkdq----ktFEPELRTLFg-MLAKGEVTVPIRNEWTLdeVPEAHRTWAQGag-vGAVVVR 333  Phaeosphaeria nodorum ...
XP_002181849  367 tpLSARFNRWRgdfv--sggKRIDLWESHqaDPELYKIRPHIAKRVMLsdVAAVHARLENGdi-rGIVVCQ 434  Phaeodactylum tricornu...
XP_383733     273 trRSTSFYYIDrdr----stYMEDLHSVMs-MLVAGKFQVPVKKTWDLenVREAHETWGKVpg-vGSCFVR 337  Gibberella zeae PH-1
XP_002293662  555 lmNSVHYDKVDryet-apneYAQHFRYLCh-LASKETIKPIVSARASLnmVAFMHKAIEHGdtpyGVCVCA 623  Thalassiosira pseudona...
XP_002293115  656 mmSQTKVYDSWenftsdpqtYQQELKYLIf-LLESGRIKPKIAERVSIeeVPDAQRYLETGka-nGTIVCL 724  Thalassiosira pseudona...
XP_002293064  976 fmDRVIAYDLMeeysedpalFTQELHYLIr-LLELGEIKAKVSGRASFkgAARAFTLVEKNka-nGTVVSL 1044 Thalassiosira pseudona...
EEU36065      267 rgKRTAFFLISrdq----pdYQPNMKALLd-LLGKSKITVPIRKIWDMddIQEAHRQWHSGsg-vGAVIIR 331  Nectria haematococca m...

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