3GAZ


Conserved Protein Domain Family
MDR6

?
cd08272: MDR6 
Click on image for an interactive view with Cn3D
Medium chain dehydrogenases/reductase (MDR)/zinc-dependent alcohol dehydrogenase-like family
This group is a member of the medium chain dehydrogenases/reductase (MDR)/zinc-dependent alcohol dehydrogenase-like family, but lacks the zinc-binding sites of the zinc-dependent alcohol dehydrogenases. The medium chain dehydrogenases/reductase (MDR)/zinc-dependent alcohol dehydrogenase-like family, which contains the zinc-dependent alcohol dehydrogenase (ADH-Zn) and related proteins, is a diverse group of proteins related to the first identified member, class I mammalian ADH. MDRs display a broad range of activities and are distinguished from the smaller short chain dehydrogenases (~ 250 amino acids vs. the ~ 350 amino acids of the MDR). The MDR proteins have 2 domains: a C-terminal NAD(P)-binding Rossmann fold domain of a beta-alpha form and an N-terminal catalytic domain with distant homology to GroES. The MDR group contains a host of activities, including the founding alcohol dehydrogenase (ADH), quinone reductase, sorbitol dehydrogenase, formaldehyde dehydrogenase, butanediol DH, ketose reductase, cinnamyl reductase, and numerous others. The zinc-dependent alcohol dehydrogenases (ADHs) catalyze the NAD(P)(H)-dependent interconversion of alcohols to aldehydes or ketones. Active site zinc has a catalytic role, while structural zinc aids in stability. ADH-like proteins typically form dimers (typically higher plants, mammals) or tetramers (yeast, bacteria), and generally have 2 tightly bound zinc atoms per subunit. The active site zinc is coordinated by a histidine, two cysteines, and a water molecule. The second zinc seems to play a structural role, affects subunit interactions, and is typically coordinated by 4 cysteines.
Statistics
?
PSSM-Id: 176233
View PSSM: cd08272
Aligned: 44 rows
Threshold Bit Score: 375.744
Threshold Setting Gi: 172058959
Created: 30-Apr-2009
Updated: 2-Oct-2020
Structure
?
Program:
Drawing:
Aligned Rows:
 
putative NAD(P)
Conserved site includes 24 residues -Click on image for an interactive view with Cn3D
Feature 1:putative NAD(P) binding site [chemical binding site]
Evidence:
  • Comment:MDR family binds NAD(P) as a cofactor
  • Comment:defined by comparison to MDR alcohol dehydrogenase like family

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
Feature 1                                                  ###  #                                
3GAZ_A         8 XIAAVVEEANGP---FVLRKLARPQPa--pGQVLVQIeASGTNPLDAKIRAGeaphaqqPLPAILGXDLAGTVVAVGPev 82  Ralstonia eutro...
YP_001133390   5 MKAVVLARFGGAd-aFELRDVAVPHVg--pRQVRVRVhATAVNPLDYQIRRGdyt-dlvPLPAIIGHDISGVIEEVGShv 80  Mycobacterium g...
YP_001639149   1 MKAMVLTRFGGPd-sFELRDLPDPRPg--pRQVRVAVhATSINPLDAQIRRGdya-dqvELPAIIGHDVSGVVDQVGPdv 76  Methylobacteriu...
NP_486988      1 MKAVLMTAPGNPe-vLQLQEVPNPGVpvgsTELLVRLvAAGINPIDTKLRKRgtf-ypeQMPAILGCDGAGVVEAVGAgv 78  Nostoc sp. PCC ...
YP_399235      6 MRAVVLEMPGGPe-qLQLQSLPWPAIah-pSEVLVQLqAAGLNPIDTKLRQRgsf-dpdRRPAILGCDGAGWVAAVGSav 82  Synechococcus e...
YP_344534      1 MKAIIMTATGGPd-vLQLQELPKPTIrq-pGEVLVQLkGAGINPVDTKLRTRgtf-ypdRSPTILGCDGAGVVDAVGRev 77  Nitrosococcus o...
NP_680792      1 MKAVAIAEFGEPd-vLVLRQVPEPQIea-aDEVKIQLrAASVNPIDTKLRQRgtf-fpdRRPAILGCDGAGVVVAVGAav 77  Thermosynechoco...
ZP_01452431   11 MRAVIMKDTGEPd-vLTLSEQEPPTCg--pDEVLVRIiAAGINPVDTKLRSRgly--cgKPPAILGCDGAGIVESIGTnv 85  Mariprofundus f...
YP_115183      1 MKAIVMTRPGEAaevLELRDLPEPALta-pTQIKVRLrAAGVNPIDTKVRRRglf-yddALPAVLGCDGAGEVVEAGAev 78  Methylococcus c...
YP_412646      1 MKAILMNAPGAPe-vLTPADVPMPDLag-aFDVRVKLhAAGVNPIDTKVRKAnmy-ypdRLPSILGCDGAGVVEAVGSsv 77  Nitrosospira mu...
Feature 1                                                          #   #                         
3GAZ_A        83 dsFRVGDAVFGLTGgvgglqGTHAQFAAVDArLLASKPAALTXRQASVLPLVFITAWEGLVDRaqv------qdGQTVLI 156 Ralstonia eutro...
YP_001133390  81 teFRAGDAVYYTPKifg-gpGSYAEQHVADVdLVGRKPDNLDHLEAASLTLVGGTVWESLVTRaql------tvGETILI 153 Mycobacterium g...
YP_001639149  77 tkFRVGDEVYYTPRifs-gpGSYAEYHVADAdIVARKPVNLTHSEAASVSLVGVTVWEGLVERarl------qvTETVLI 149 Methylobacteriu...
NP_486988     79 qgFRVGDAVYFCNGglgghqGNYAEYTIVDErFAAHKPASLTFAEAAAAPLVLITAWEALYERgrl------epKERVLI 152 Nostoc sp. PCC ...
YP_399235     83 drFAVGDAVYFCHGglglepGTYADYAVVDQaWLAPKPERLSFVEAAGVPLALITAWEALYDRgrl------qaAQTVLI 156 Synechococcus e...
YP_344534     78 knFQKGDEVYFCFGgiggpeGNYGEYAVVDHrFIAKKPRTLSFAEASAAPLVLITAWEALHDRari------qpEDTVLI 151 Nitrosococcus o...
NP_680792     78 rrFRVGDEVYFCYGglgdrgGCYAEYAVVPEaAVAHKPKTLSFIQAAALPLAVITAWEALGDRgavpplnvlstAKTVLI 157 Thermosynechoco...
ZP_01452431   86 srFEPGDAVYYCYGglgqtaGNYAEYIAVPEpYVAQKPDSLDFMDAAAAPLVLITAWEALFDRahi------qsGQHVYI 159 Mariprofundus f...
YP_115183     79 drFRIGDRVWFCNGglgaeqGNYAEYTVLDQrWAAPMPASSAFASAAAGPLVLITAWGALYDRgrl------qpGQTVLV 152 Methylococcus c...
YP_412646     78 trVRAGDEVFFFNNglggapGNYAEYAVVHEdYLALKPGNLSMVEAAAVPLALITAWEALIKRgnl------kgSQIALI 151 Nitrosospira mu...
Feature 1         #### ##                 ##   #             #                        ## #       
3GAZ_A       157 QGGGGGVGHVAIQIALARGARVFATARG-SDLEYVRDLGATPIDASRe--pEDYAAEHTAGQGFDLVYDTLGGPVLDASF 233 Ralstonia eutro...
YP_001133390 154 HGGAGGVGTIAIQVAKAMGARVITTAKA-GDHAFVNSLGADVAIDHTstdyVDAVAEATRGRGVDVVFDTIGGDTLTRSP 232 Mycobacterium g...
YP_001639149 150 HGGAGGVGAIAVQVAKAIGARVLATARG-SDAALVEQLGASAVIDFEredyVDAVNRLTEGRGVDVVLDTIGRDTLSRSP 228 Methylobacteriu...
NP_486988    153 HAGAGGVGHVAIQLAKLKGAIVSTTVGSeDKANFVKQLGADHVILYKntdfAQAALDWTNGEGVDLAFDTVGGETFHKTF 232 Nostoc sp. PCC ...
YP_399235    157 HAGAGGVGHLAIQLAKLRGARVATTVSTpEKAAIAEHYGADFVIPYRdrdwVAAVLDWTDGKGVDLAFDTVGGAVLEQTF 236 Synechococcus e...
YP_344534    152 HGGAGGVGHVAIQLAKQTGARVCVTVSCeEKEELACSLGADHIINYRqtdfVEAIMEWTSGKGVDVVFDTVGGEIFEKSC 231 Nitrosococcus o...
NP_680792    158 HAGAGGVGHLAIQLARRAGAQVATTISSpAKAQFTEALGATLAINYTttdwVQAVLDWTGGKGVDLALDTVGGATFAETF 237 Thermosynechoco...
ZP_01452431  160 AGGAGGVGHVAIQLAKLAGCHVATSVSSdEKADVVRELGADLIIHYRsqdvAEALLAWTDGTGVDVAFDTVGGEAFNQLV 239 Mariprofundus f...
YP_115183    153 HAAAGGVGHVAVQLAKLRGARVIATVGSeDKAALARGWGADETIDYRtqdfVAEVNALTGGRGADLVLDTVGPEVFRRSV 232 Methylococcus c...
YP_412646    152 HAGAGGVGHIAIQLARYLKARVATTISSeEKAAFVQSLGAELAIDYRendfVDTALEWTEGLGVNLALDTVGGETFCKSF 231 Nitrosospira mu...
Feature 1                   ##                     ###                                           
3GAZ_A       234 SAVKRFGHVVSCLgwg--thkLAPLSFKQATYSGVFTLhtllan--eglahFGEXLREADALVQTGKLAPRLDpRTFSIA 309 Ralstonia eutro...
YP_001133390 233 LSLADSGRVVSIVdiar-pqnLIEAWGRNAAYHFVFTRq------------NRGKLDALTMLVERGLVKPVLG-ATLPLA 298 Mycobacterium g...
YP_001639149 229 HILREDGRVVTLVdtat-pqnLIDAWGRNATYHFVFSRq------------NGSKLDALTHLLERGLVVPQVG-LEFPLD 294 Methylobacteriu...
NP_486988    233 PAVRIYGDIVTILepda-ntvWKVARNRNLRIGLELMLtpmllnlveaqqhQAEILTECADWIDQGKLKIHVS-HKFPLQ 310 Nostoc sp. PCC ...
YP_399235    237 PAVRLYGDLVTILapda-atnWKVARDRNLRISLELMLtpqlqrvtearqhQTQILTTASRWFDQEQLHIHIG-QVLPIS 314 Synechococcus e...
YP_344534    232 GAVAMYGDLVTLLqpsa-ninWNTARARNLRFSLELMLtpmhrglisalehQADILHCCAELFDSERLRLHFQ-QTFPLA 309 Nitrosococcus o...
NP_680792    238 KAVRPYGTLATLLepav-dtpWKIARQRNLLIQLTLMLtpqllglrealahQGQILEQVATLVDRGELRVVVD-KTFPLG 315 Thermosynechoco...
ZP_01452431  240 PATRVYGDIVTILqvpd-dadWKSIRLRNIRISHELMLtpmvlglheaaehHGDILEQCAQFFDERKLSVFVS-DVLPLE 317 Mariprofundus f...
YP_115183    233 ECTAHFGDLVTLLdtg--atsLAEARMRNLRIGFELMLvpmlrkldaardhHVEILRACGEWIDDGKLELYVS-GVHPLE 309 Methylococcus c...
YP_412646    232 SAIRLYGRVVSLLstvcdakqLNTARLRNLSIGYVQMTaplyfglhsarvvQTGILEQGARLLEQGILKIHVS-RTLPLT 310 Nitrosospira mu...
Feature 1                                  
3GAZ_A       310 EIGSAYDAVLGRNdvprqRGKIAITV 335 Ralstonia eutropha JMP134
YP_001133390 299 RMGEAHELLENRRsy-alRGKVAIDV 323 Mycobacterium gilvum PYR-GCK
YP_001639149 295 DVARAHARSEEGRl----KGKIVLRV 316 Methylobacterium extorquens PA1
NP_486988    311 DAAKAHQLLESGSi----AGKIVLLI 332 Nostoc sp. PCC 7120
YP_399235    315 EISQAHQFLERQQg----AGKIVIDL 336 Synechococcus elongatus PCC 7942
YP_344534    310 EAAAAHRLLERGGm----MGKLALEM 331 Nitrosococcus oceani ATCC 19707
NP_680792    316 AAADAHRYLSQRLv----QGKVVLIP 337 Thermosynechococcus elongatus BP-1
ZP_01452431  318 QAAEAHRRIEAGSm----SGKLVLDI 339 Mariprofundus ferrooxydans PV-1
YP_115183    310 EAARVHDIIEAGHt----TGKRVLCI 331 Methylococcus capsulatus str. Bath
YP_412646    311 EAAEAHRLIEAGHt----LGKIVLKI 332 Nitrosospira multiformis ATCC 25196

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap