3GOH


Conserved Protein Domain Family
MDR5

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cd08271: MDR5 
Click on image for an interactive view with Cn3D
Medium chain dehydrogenases/reductase (MDR)/zinc-dependent alcohol dehydrogenase-like family
This group is a member of the medium chain dehydrogenases/reductase (MDR)/zinc-dependent alcohol dehydrogenase-like family, but lacks the zinc-binding sites of the zinc-dependent alcohol dehydrogenases. The medium chain dehydrogenases/reductase (MDR)/zinc-dependent alcohol dehydrogenase-like family, which contains the zinc-dependent alcohol dehydrogenase (ADH-Zn) and related proteins, is a diverse group of proteins related to the first identified member, class I mammalian ADH. MDRs display a broad range of activities and are distinguished from the smaller short chain dehydrogenases (~ 250 amino acids vs. the ~ 350 amino acids of the MDR). The MDR proteins have 2 domains: a C-terminal NAD(P)-binding Rossmann fold domain of a beta-alpha form and an N-terminal catalytic domain with distant homology to GroES. The MDR group contains a host of activities, including the founding alcohol dehydrogenase (ADH), quinone reductase, sorbitol dehydrogenase, formaldehyde dehydrogenase, butanediol DH, ketose reductase, cinnamyl reductase, and numerous others. The zinc-dependent alcohol dehydrogenases (ADHs) catalyze the NAD(P)(H)-dependent interconversion of alcohols to aldehydes or ketones. Active site zinc has a catalytic role, while structural zinc aids in stability. ADH-like proteins typically form dimers (typically higher plants, mammals) or tetramers (yeast, bacteria), and generally have 2 tightly bound zinc atoms per subunit. The active site zinc is coordinated by a histidine, two cysteines, and a water molecule. The second zinc seems to play a structural role, affects subunit interactions, and is typically coordinated by 4 cysteines.
Statistics
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PSSM-Id: 176232
View PSSM: cd08271
Aligned: 21 rows
Threshold Bit Score: 328.466
Threshold Setting Gi: 154333157
Created: 14-Aug-2009
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
putative NAD(P)substrate
Conserved site includes 24 residues -Click on image for an interactive view with Cn3D
Feature 1:putative NAD(P) binding site [chemical binding site]
Evidence:
  • Comment:MDR family binds NAD(P) as a cofactor
  • Comment:defined by comparison to MDR alcohol dehydrogenase like family

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                     ###  #                             
3GOH_A         5 HQVWAYQTKt-------hSVTLNSVDIPALa-aDDILVQNQAIGINPVDWKFIKANPINWSn------------------ 58  Shewanella onei...
YP_001814406   2 MKAWLNHTGea-----idQWTFEEIETPTPg-aGQALIRVKSVALNPVDYKAIKNPAWTYP------------------- 56  Exiguobacterium...
XP_847228     31 CHGWMVSKCvrqw--kaaAAINSEITIPPPg-kGQVRVKLYAAAVNPVDAKRAAFGYQVSSslftgglsrgtgvastmlg 107 Trypanosoma bru...
XP_001562839  46 CAGWIAPKGnpsw-srrnVVYSSDIEIPVPq-sRQVRVKVYAAGVNPTDVHRTAVLPRSPEvgsrgkgrhtt------rh 117 Leishmania braz...
ABK81657       1 MKAISYNDKn-------dIFEYIEKEVPKLdknLDVLVKVIAVGLNPVDAKINQWASMRKEpl----------------- 56  Francisella tul...
YP_001447387   1 MKAITYQKDq-------dTFTYTDIDTPALasgLDVLVKVHAVGLNPVDAKVNLWHEMVDGmd----------------- 56  Vibrio harveyi ...
NP_783953      1 MQALVVPDPsaq---tmeQLQWQERPMPNLg-pTEVLIKTQAVGLNPVDFKLVAAGNAAWQy------------------ 58  Lactobacillus p...
XP_001562838  14 CKGWSVEKPalqwstdavKLLDVTVPAPGP---TQIRVKVHAASINPIDWKRLMFSPSGSGlgvagsglagfk---gmhr 87  Leishmania braz...
YP_002885798   1 MKAWLLDQPgl------eHLHIGEIDRPTPg-tGELLIRVEAVALNPVDYKVGSNGNPNWAy------------------ 55  Exiguobacterium...
YP_002886458   1 MKALLLHEPnt-----wqTMRIEEVDTPTLr-sGEVLIEGEYAGLNPVDYKTATNGKPEWSy------------------ 56  Exiguobacterium...
Feature 1                                                                                        
3GOH_A        59 -------gHVPGVDGAGVIVKVGakVDSKx-------------------LGRRVAYHTSLk--rHGSFAEFTVLNTd--- 107 Shewanella onei...
YP_001814406  57 --------HVPGVDLTGIVEQTGpnVTGIk-------------------VGERIAVHTNLq--kNGAFAEYAVVDAr--- 104 Exiguobacterium...
XP_847228    108 petlfpfpYVMGVEGAGVIESVG--WDSSkeqq------------ydfqVGDRVAFLADMsqpfGGTFCQYAVVHAdalg 173 Trypanosoma bru...
XP_001562839 118 prppfkfpYVVGIEGAGVVESVGw-AAGSngeeddsagalasagasdihVGDRVAFLADFtqgsGGTFCQYAVVDSdilw 196 Leishmania braz...
ABK81657      57 ------sqWVCGLDVYGEIVEIGkeVSGWh-------------------KDDLVLYHGDMmr-aNGGFAEYAIQDSr--- 107 Francisella tul...
YP_001447387  57 ------dtFVGGLDVSGEIVAIGdkVSGWq-------------------VGDRVLYHGNMrr-mSGGFAEYAIQDSr--- 107 Vibrio harveyi ...
NP_783953     59 -------pHTLGLDTAGIVEAVGaeVTNFr-------------------PGERVCGHANLa--nNGTFAEYVSLPAa--- 107 Lactobacillus p...
XP_001562838  88 kppnypypYVVGVDGAGEVESVGekVTELk-------------------VSDRVMFHSSLtktyGGSLCEYAVMEEsva- 147 Leishmania braz...
YP_002885798  56 -------pHILGVDLVGEVVEVGssIANIi-------------------TGSRVAVHTDLs--kYGGFAEYTVVDAr--- 104 Exiguobacterium...
YP_002886458  57 -------pHVLGLDVAGTISAVAddVTNVq-------------------VGDRVLYHSNWn--eWGGFAEFTRAKAh--- 105 Exiguobacterium...
Feature 1                                        #   #                    #### ##                
3GOH_A       108 --------------rVXTLPDNLSFERAAALPCPLLTAWQAFEKIPlt-kQREVLIVGFGA-VNNLLTQXLNNa-GYVVD 170 Shewanella onei...
YP_001814406 105 --------------aLAKIPDAVSFSEAAAILCAGMTAYEAIIQKMnttgKETILIHAGAGgVGGIGIQLAKRl-GLAVA 169 Exiguobacterium...
XP_847228    174 -----------kipaPTDGNDFIDFVEASTMPCSAGAAYVALFDKLrverGRSIFISGASGgVGSVAVQLAKYv-GLCVL 241 Trypanosoma bru...
XP_001562839 197 klpevvcsppfpstgGLMPGRLIDFVEAASLPAAAATAYIALFDKLrietQRSIFISGASGgVGSVAVQLAHYf-GLYVI 275 Leishmania braz...
ABK81657     108 --------------tIIKASSSLTAVEQASIPCAGWTAYRAMIEKLniskDDNVFISGGSGgVGSFAVQLAKNagAKHII 173 Francisella tul...
YP_001447387 108 ---------------TLIAHPDVAPEVAAASPCAGWTAYRALVDKLnistRKSIFIAGGSGgVGSFAIQLARFfgVETII 172 Vibrio harveyi ...
NP_783953    108 --------------vLAKIPTTVSFEAAAASLCAGLTAYQALLRKAnlnaAQTVLIHAGAGgVGSMAIQLAKAa-GKTVF 172 Lactobacillus p...
XP_001562838 148 ------------vpiPTDGAKSFSYEEAAAIPCATWAVYVALFDKLriepGRSIFVDGASGaVGSSAVQLAHHm-GLYVF 214 Leishmania braz...
YP_002885798 105 --------------aCAVVPQGVSDEAAASILCAGMTAYEAVMQKLntrdKETILVHAGAGgVGGFAIQLAKRl-GLKVF 169 Exiguobacterium...
YP_002886458 106 --------------mVVPIPESISFADAVALPTAGFTAYQAIFEKLpmnaIDTILIHAGAGgVGGFAIQLAKYl-GKRVI 170 Exiguobacterium...
Feature 1          ##  #              #                        ## #                  ##          
3GOH_A       171 LVSASlSQALAAKRGVrHLYREPSqvt-----------qkYFAIFDAVNSqnAAALVPSLkaNGHIICIQDRIpap---i 236 Shewanella onei...
YP_001814406 170 TTASTeNHDWVKKLGAdLAIDYKTenvtevir-dwtngrgADLIFNTVGRdeATADLGRLafSGQLAFIAGGPdqs---v 245 Exiguobacterium...
XP_847228    242 ASCSTvNVPYVSSLGAdFVFDYTTnnvvkecl-ehtqsvgVDYVLELADAalAAKHAEALrfGGSICVLPGPMnandans 320 Trypanosoma bru...
XP_001562839 276 ASCSTpNVRYVQNLGAdYVVDYTRtdvvkdil-mytdnygVDYLLECADAsmAEAHSETVrlGGSLCVLTGLFtps---s 351 Leishmania braz...
ABK81657     174 TSCSEkNFEYVKSLGAtHVINYNDknliekvi-dytdgygIDKLLNTSGRstDITLSDLLafNGHFLELLDVVrpt--ey 250 Francisella tul...
YP_001447387 173 TTSSGaNHEFVRSLGAtHVIDYKQedvreqim-aitegfgVEVSLDCVGGdnDILCASVLgfEGEMVELVRTTrat--ey 249 Vibrio harveyi ...
NP_783953    173 TTVSKaKQAFVAPLHPdAQIDYHHedvttriq-dltqqhgVDLIINTIGH--ADADLPRLayNGQLVCVLATPd------ 243 Lactobacillus p...
XP_001562838 215 ASCSPsNVDYVRSIGAdHVLNYHDgldmidqildategygVDYYLAVTNTqdAENFTDTLr-FGGAVCLLSGVlip--qs 291 Leishmania braz...
YP_002885798 170 TTASPeNFEWVESLGAdVVIDYNVedvtvrim-eetdgrgVDLILNTVGRdvATADLDRLafSGHLAYIAGPPdmt---n 245 Exiguobacterium...
YP_002886458 171 TTASArNHDWVKQLGAdEAIDYNTedwqqrvm-eltsghgVNAVIDPVGRetATASLEVIayNGHIAHIAGAPdms---v 246 Exiguobacterium...
Feature 1                    ###                                                                 
3GOH_A       237 dPAFTRTISYHEIALGALHDFGDrqDWQILXQQGEALLTLIAq-GKXEIAAP-DIFRFeq-------------------- 294 Shewanella onei...
YP_001814406 246 vKPFTLSPSIHEVALAAAYAIEDdrAIRNLGHMADELLKLVAa-KELDPLVT-EEIPAad-------------------- 303 Exiguobacterium...
XP_847228    321 gVFFRKQISVSYVCLAGLYGSEV--TRKLLRSVVEESLRLYQ--NGAFRLHV-ETASIervrdvmdvvasghargkivlt 395 Trypanosoma bru...
XP_001562839 352 dMVFRRQLSVHYVFLGLQHQDPL--ARMQLKPLGELVMQLYI--QGAFSVNV-EQVPF---------------------- 404 Leishmania braz...
ABK81657     251 kDVFMRNLSFHQLSLGAVLDMGQpvKIIDLAKQFIRLSGRDDidIKIVGLRPgEKLYEelliedddvstdykd-ifigrr 329 Francisella tul...
YP_001447387 250 pGAFLQGLTFHQLSLGSGHVHGEk-GFASIVNAGVEFSHMLEr-GEVMVPKL-EVVALkd-------------------- 306 Vibrio harveyi ...
NP_783953    244 hFPSNQVVTISKLDLGGAHRSGNpaQVADLGQMTAALLALVQa-GKVDPLIT-QVLHRdql------------------- 302 Lactobacillus p...
XP_001562838 292 dVLFRRQLSVHYVFLNGLHDHPL--TRPQLRYIGDQVAKLYL--SGAFRLDM-EVLPF---------------------- 344 Leishmania braz...
YP_002885798 246 mKGFTLSPSIHEVALGAAHASEDehAIRNLAYMAEEMMNLLDa-GELNDLVT-EVLSFdq-------------------- 303 Exiguobacterium...
YP_002886458 247 iKPFTRVISYHEVALNAIHHQQDrvSEANIVRIGTHMLELLAa-GEIQPMIQ-ETIPFad-------------------- 304 Exiguobacterium...
Feature 1                                         
3GOH_A       295 --------xIEALDHSEq------tKLKTVLTL 313 Shewanella oneidensis MR-1
YP_001814406 304 --------lVKGLQRLSer----hvRGKLIATF 324 Exiguobacterium sibiricum 255-15
XP_847228    396 dfhptespgVDAAVGSR--------RRKQVRDA 420 Trypanosoma brucei TREU927
XP_001562839 405 ---------VQATDALEvvas-ghgRGKIVLTN 427 Leishmania braziliensis MHOM/BR/75/M2904
ABK81657     330 tfydintlnQDIESLIKddvdqlviLKKIVPEF 362 Francisella tularensis subsp. novicida U112
YP_001447387 307 --------vAQALVSIRqq----rtVGKIVAKL 327 Vibrio harveyi ATCC BAA-1116
NP_783953    303 ------vtgLQQLQAGQv-------VGKLVVTF 322 Lactobacillus plantarum WCFS1
XP_001562838 345 ---------AEAAEALDlsatgnvnHKKLVVQV 368 Leishmania braziliensis MHOM/BR/75/M2904
YP_002885798 304 --------lKEGLEKLQtr----kvRGKLIVRI 324 Exiguobacterium sp. AT1b
YP_002886458 305 --------iPKKLVELSdr----hvVGKIVANL 325 Exiguobacterium sp. AT1b

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