Conserved Protein Domain Family
MDR4

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cd08270: MDR4 
Medium chain dehydrogenases/reductase (MDR)/zinc-dependent alcohol dehydrogenase-like family
This group is a member of the medium chain dehydrogenases/reductase (MDR)/zinc-dependent alcohol dehydrogenase-like family, but lacks the zinc-binding sites of the zinc-dependent alcohol dehydrogenases. The medium chain dehydrogenases/reductase (MDR)/zinc-dependent alcohol dehydrogenase-like family, which contains the zinc-dependent alcohol dehydrogenase (ADH-Zn) and related proteins, is a diverse group of proteins related to the first identified member, class I mammalian ADH. MDRs display a broad range of activities and are distinguished from the smaller short chain dehydrogenases (~ 250 amino acids vs. the ~ 350 amino acids of the MDR). The MDR proteins have 2 domains: a C-terminal NAD(P)-binding Rossmann fold domain of a beta-alpha form and an N-terminal catalytic domain with distant homology to GroES. The MDR group contains a host of activities, including the founding alcohol dehydrogenase (ADH), quinone reductase, sorbitol dehydrogenase, formaldehyde dehydrogenase, butanediol DH, ketose reductase, cinnamyl reductase, and numerous others. The zinc-dependent alcohol dehydrogenases (ADHs) catalyze the NAD(P)(H)-dependent interconversion of alcohols to aldehydes or ketones. Active site zinc has a catalytic role, while structural zinc aids in stability. ADH-like proteins typically form dimers (typically higher plants, mammals) or tetramers (yeast, bacteria), and generally have 2 tightly bound zinc atoms per subunit. The active site zinc is coordinated by a histidine, two cysteines, and a water molecule. The second zinc seems to play a structural role, affects subunit interactions, and is typically coordinated by 4 cysteines.
Statistics
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PSSM-Id: 176231
View PSSM: cd08270
Aligned: 11 rows
Threshold Bit Score: 268.471
Threshold Setting Gi: 158315467
Created: 20-Oct-2009
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
putative NAD(P)
Feature 1:putative NAD(P) binding site [chemical binding site]
Evidence:
  • Comment:MDR family binds NAD(P) as a cofactor
  • Comment:defined by comparison to MDR alcohol dehydrogenase like family

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                ###  #                                  
ACU38270       1 MRALVVDHAGpgPVRFA--EVAEPEPAPHEALVGIRHIALNHGELKhsg--qwPDGDVHGHDASGVVlRAAADGSGPEAG 76  Actinosynnema m...
ACU37724       1 MRALLVDHSV--PRHLRqgQAPDPVPTPGQALVRVVATSLNPGELAgla--dlPGGAVPGWDAAGVVvRAAEDGSGPAEG 76  Actinosynnema m...
ABJ96324       1 MRAIVYDPLA--PANLRfaEVDEPAVAAHEALVDVQAIALNFGELHfid-harKPGEIPGWDTAGIVaQAAADGSGPPVG 77  Mycobacterium s...
YP_001869283   4 IRAVIVDPNV--PGRLAlsEVNAPKPAPDEALVRVAAISLNRGEIKrst--taEAGWQPGWDLAGVVeIPAADESGPPQG 79  Nostoc punctifo...
YP_001521503   1 MQRIIMNSDQegGLSFV--ETDSLQPLPHQSLIKVTAFSLNRGEINiaq-sptSGGRSIGWDFVGVIeNSAADGTGLKKG 77  Acaryochloris m...
CAJ87974       1 MRALLVDRSA--PAGLRlgPAPDPRPAPHQALIRVTATSLNYGEVRfal-rdaPEGAVLGWDAAGVVlEAAADGSGPAAG 77  Streptomyces am...
YP_003298353   3 MRALIVDPDA--PTALRfgTVPEPDPAPGQLVLEVRHISLNRGEVTfaa--qrPAGTVHGYDAAGIVvRAAADGTGPSVG 78  Thermomonospora...
YP_001109255  13 MRALLITPGTasGLSLG--DTLDPVPAANEVLVDVEHVSLNYGEIGfarapeaERGAVHGWDAAGVVaRAAADGSGPAVG 90  Saccharopolyspo...
YP_003241102   2 IRAIVVDPLA--PSHLAfkEVEAPQAQPWEALVQVKTVSLNRGEVKdvk--eqEASRRPGWDFAGIViEPAKNGAGPQKG 77  Geobacillus sp....
NP_104388      1 MLALTISSDS--AARLKlaEVDEPQPCANEALVAVHATSLNRGELRllt--irPDGWIPGQDIVGIVeQAAADGSGPAKG 76  Mesorhizobium l...
YP_001507975   1 MRALVTDGSAagGLRLG--EVPDPVPGPDQVLIRTAAISLVDRDTGyaa-amlGDGGVWGFDAAGVVvEAAADGSGPPVG 77  Frankia sp. EAN...
Feature 1                                                #   #                   #### ##         
ACU38270      77 SRVVLgm-apHAWAERIAVAPKWLAVVPDGVGLADAAAIGVVGSTALRVLRTRS-VLGREVLVTGASGGVGRFAVQLAAL 154 Actinosynnema m...
ACU37724      77 TPVVTlg-laGAWAELRAVDTAHLGPVPEGADLGPVATVPVAAGSALRALHRLGpLLARRVLVTGATGGVGRYALQLARR 155 Actinosynnema m...
ABJ96324      78 ARVVGln-fgGGWAQRRAIATENLAVLPEFVEFEEAAALPVAGVTALQALRALGpVVGRRVLITGASGGVGRFAVQLAAR 156 Mycobacterium s...
YP_001869283  80 ARVVGlr-raGAWAELVSVPTNALAELTPSVSFAQAATLPVAGLTAYHAVLKGGsLLGRPVLVTGASGGVGYFAIQLAQL 158 Nostoc punctifo...
YP_001521503  78 TRVVGwrpemDAFAEYVIGEHQFIAPIPDAVSDVQAATLPVAGLTALAAVDQGNrLVGNSALVTGVTGGVGYFALQLAKL 157 Acaryochloris m...
CAJ87974      78 TPVVTlg-vdGAWAELRAVDTAWIGTARAGSDPGAVSTVPVAGTSALRALHRLGpVLGRRVLITGATGGTGRYAVQLARR 156 Streptomyces am...
YP_003298353  79 ARVAAlg--tGAWAQRMAVDTTAVAEVPDGVDLADAAALPVAGVTALRALRTRP-ILGRRVLVTGAAGGVGRYAVQLAAL 155 Thermomonospora...
YP_001109255  91 SRVVTfg-ggGAWAQLRAVPVGELAVVPDDVSLAVASAVPVAGVTALRALRRSGpLLGRRVLVTGASGGVGRFAVQLAAR 169 Saccharopolyspo...
YP_003241102  78 ARVVGll-pmGAWSEQVAAPVSMLAQIPDKLTFTEAATLPVAGLTALYALRKGGmLLGKRIFITGSTGGVGLFAHQLAAQ 156 Geobacillus sp....
NP_104388     77 ARVVAlv-deAGWAERVAVPTDRLAVLPDEVSFVSAATLPVAGTTALRTLRQGGdLAGQRVLITGASGAVGRFQIQIARE 155 Mesorhizobium l...
YP_001507975  78 STVLTllpapGAWAELVTASTGDVAMLPPGVDPGVLTGLALPAVSAVQALGEVEgLAGSRVLVTGAGAGVGWFAVQLAAL 157 Frankia sp. EAN...
Feature 1                ##    #                   #                                ## #         
ACU38270     155 aGARVSALVGsp-eRVEGLRelg-----arRVAVSVSELAG-------------------EFDLVVDTVGGPQLVEVWER 209 Actinosynnema m...
ACU37724     156 aGAHVVAATSdpaaHAEGLRal------gaHEVVGPDLATG-------------------PVHGVVELVGGPALVAAHAL 210 Actinosynnema m...
ABJ96324     157 aGAHVIAAVGsp-aRGAGLEelg-----aaEVTVGLDEISE-------------------PVFGVLDNVGGPLLAQAFRL 211 Mycobacterium s...
YP_001869283 159 sGAQVTAHIRq--aGYETLVrea-----gaQSVVIGEDLSPase--------------ygPYHLIIESVGGKTLGVALGL 217 Nostoc punctifo...
YP_001521503 158 sGARTVAQVR----RSEQATfasnigadavVVTEDGSGLEGe-----------------gPYHLVVDGVGGQLFSSLLSV 216 Acaryochloris m...
CAJ87974     157 aGAYVIASTGnprkHGEVLLaqg-----ahEVVAGPGDIAD-------------------PVHGIVDMVGGPQLVEYFAL 212 Streptomyces am...
YP_003298353 156 gGAHVIASVGsa-aRTAGLAdlg-----arQVVIGLDGIDR-------------------PVDLILDTVGGPQLAAAWRL 210 Thermomonospora...
YP_001109255 170 gGAHVVAVSG----RGAGLAel------gaHEVVSSLDDVG-------------------PLDVVIDNVGGPQLVRAWEL 220 Saccharopolyspo...
YP_003241102 157 sGAYIVGTASte-eKAELVReag-----aeEVIIGYSAISSahq--------------fgPYDLIIDSVGGDTLAALLPQ 216 Geobacillus sp....
NP_104388    156 qGASVTAVAAa--rHAGDLRdl------gaEQVVEAIELAEg------------------PFSLITESVGGESLARAIER 209 Mesorhizobium l...
YP_001507975 158 rGAEVVAVARdp-aDADDLRaa------gaHEVRTELPATDpgdpvggdpataepaaslrPVDVVVDVVGGSTMTRAVDL 230 Frankia sp. EAN...
Feature 1                 ##                        ###                                          
ACU38270     210 VAPGGDVQLVGTASGqpaVFGPDALFsi--gEPKTVTTFCDve----sAVTGELEVLLDLMArgrLSAEVGLRGHWdGVD 283 Actinosynnema m...
ACU37724     211 LRPGGTLVSVGRASGgqsVFDGAAFEgt-fqPDRVIATFFLpas--gpGLDEDLGWLAARVAsgeLDAGVAWRGSWaRAD 287 Actinosynnema m...
ABJ96324     212 VGDGGSVQSIGMASNeptTINFEEERrl--gYRKRLEPFVIr-----tPIAGDLSYLLTLLAdgeLDPQIGLRDSWdNIS 284 Mycobacterium s...
YP_001869283 218 LAQDGKTVLYGVSGGaevTFNAAQFFg---tGSVSLYGLRLfdelrfeSAAVGLKRLLSLVEvgqLHPHIDLEASWtQIA 294 Nostoc punctifo...
YP_001521503 217 TAKGGTLVSYGSSEShesTVNLYPALfg-ngGQRRIYGLTLyseiekePSSAALTRLLNLVErdlLKLPETITSDWsETP 295 Acaryochloris m...
CAJ87974     213 LTQGGTLVAVGHAAHegeSFPYGALFgdggrHDRSLVTFSLpa---ctGLAPDLGWLADEVAlgrLDPNISWRGGWdRAA 289 Streptomyces am...
YP_003298353 211 LAPGGDVQSIGWASGepaVFEPYSLYsp--gPAKTISTFGDa-----dEPGPDLAVLLGFVAdggLSPEIGWRGSWeRFD 283 Thermomonospora...
YP_001109255 221 LEPGGVVQSIGWTSGepaVFQPYSTVg----PAKSLTAFQMg-----sDLSADLEYLTSLIAagaLTVDIGWRGSWlRYD 291 Saccharopolyspo...
YP_003241102 217 LAPQGICVALGFSSSniaTIDMSNLVt---rGGRTLYSFFLgdelnrqSAADDLSLLAGLVAdgrLTPRIEVETPWtEID 293 Geobacillus sp....
NP_104388    210 VAPGGTIAMFGSSSGqltPIGFRQFVpg--hEGARLQTFAYyts--gfGIGADIAALLALVAagrLETRVALTVPWtDIA 285 Mesorhizobium l...
YP_001507975 231 LAEGGTALAVGAISGermVFPPAAFAs---pLRRRVRGFWGs-----wPVGGDLATVVELVAagrLCPRPGWRGGWgEVT 302 Frankia sp. EAN...
Feature 1                            
ACU38270     284 GAVRALFA-REVRGKVVLDV 302 Actinosynnema mirum DSM 43827
ACU37724     288 EAVAGLLD-GRLRGKAVLEV 306 Actinosynnema mirum DSM 43827
ABJ96324     285 TAAAALLG-RTVAGKAVLRV 303 Mycobacterium smegmatis str. MC2 155
YP_001869283 295 DVAQQLLD-RRFPGKAVLHI 313 Nostoc punctiforme PCC 73102
YP_001521503 296 HLAEDLLA-RKFTGKAVLTL 314 Acaryochloris marina MBIC11017
CAJ87974     290 DAVDALLN-RRLHGKAVLEI 308 Streptomyces ambofaciens ATCC 23877
YP_003298353 284 EAARALLE-RRVAGKAVLDV 302 Thermomonospora curvata DSM 43183
YP_001109255 292 EAAEALLG-REVTGKAVLDV 310 Saccharopolyspora erythraea NRRL 2338
YP_003241102 294 SVARSLME-RKFSGKAVLHL 312 Geobacillus sp. Y412MC10
NP_104388    286 QALDALRQ-RSFSGKAVLTM 304 Mesorhizobium loti MAFF303099
YP_001507975 303 GLLESFAAgRTRRRRAVLDV 322 Frankia sp. EAN1pec

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