Conserved Protein Domain Family
MDR2

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cd08268: MDR2 
Medium chain dehydrogenases/reductase (MDR)/zinc-dependent alcohol dehydrogenase-like family
This group is a member of the medium chain dehydrogenases/reductase (MDR)/zinc-dependent alcohol dehydrogenase-like family, but lacks the zinc-binding sites of the zinc-dependent alcohol dehydrogenases. The medium chain dehydrogenases/reductase (MDR)/zinc-dependent alcohol dehydrogenase-like family, which contains the zinc-dependent alcohol dehydrogenase (ADH-Zn) and related proteins, is a diverse group of proteins related to the first identified member, class I mammalian ADH. MDRs display a broad range of activities and are distinguished from the smaller short chain dehydrogenases (~ 250 amino acids vs. the ~ 350 amino acids of the MDR). The MDR proteins have 2 domains: a C-terminal NAD(P)-binding Rossmann fold domain of a beta-alpha form and an N-terminal catalytic domain with distant homology to GroES. The MDR group contains a host of activities, including the founding alcohol dehydrogenase (ADH), quinone reductase, sorbitol dehydrogenase, formaldehyde dehydrogenase, butanediol DH, ketose reductase, cinnamyl reductase, and numerous others. The zinc-dependent alcohol dehydrogenases (ADHs) catalyze the NAD(P)(H)-dependent interconversion of alcohols to aldehydes or ketones. Active site zinc has a catalytic role, while structural zinc aids in stability. ADH-like proteins typically form dimers (typically higher plants, mammals) or tetramers (yeast, bacteria), and generally have 2 tightly bound zinc atoms per subunit. The active site zinc is coordinated by a histidine, two cysteines, and a water molecule. The second zinc seems to play a structural role, affects subunit interactions, and is typically coordinated by 4 cysteines.
Statistics
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PSSM-Id: 176229
View PSSM: cd08268
Aligned: 34 rows
Threshold Bit Score: 277.558
Threshold Setting Gi: 255922451
Created: 28-May-2009
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
putative NAD(P)
Feature 1:putative NAD(P) binding site [chemical binding site]
Evidence:
  • Comment:MDR family binds NAD(P) as a cofactor
  • Comment:defined by comparison to MDR alcohol dehydrogenase like family

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                               ###  #                                   
EEH93023       2 SKVVVFNQIGGaEVLKIQemqVPAPDANEVQIRVrAIGINRAEVMYRTGQYiyq-pnFPARLGYEAAGVIESVGDNVReF 80  Citrobacter sp....
YP_002945770   2 GRMVRFHRFGGaDVLKVEqvaTPEPGAGEVRIAVqAIGLNWADALWRQNQYied-piLPAGLGNEAAGLIEAVGAGVDgL 80  Variovorax para...
YP_002947569   6 MKAIQLQAPGGpEVLQLVelpLPQPQAGEVRVKAmAIGAGGPDVLIRNGTYkwm-ppMPAIPGNEMAGIVDAIGPGVTaL 84  Variovorax para...
YP_001262158   1 MKLVCVSEFGPpEALRIVdapEPVPAAGQALVAIeTIGLGYMDTAARRGESylv-ssPGFVPGYEVAGTVAAIGNGVDaS 79  Sphingomonas wi...
ACU37962       1 MRAVTVGGFGGpEVLVVGevpDPVPGSGRVLVRTeAIGVGGVDAVLRRGTLgld-fpAGLPLGSEVAGTVTAVGAGVDpG 79  Actinosynnema m...
YP_003315528   1 MRAVTIQQFGDpSGMVVAelpPPVPPADHLVVQTeAIGVGGVDAVVRRGALgpa-fsEGMILGSEVAGTVSSVGAGVDpA 79  Sanguibacter ke...
YP_001106330   1 MKAVAIRTFGPpEGLAVIdlpAPTPAAGQVLIETeAIGVGGVDALIRSGALasygfeEGHILGSEVAGTVTAVGDGVAaS 80  Saccharopolyspo...
NP_769719      1 MRAVLVRQPGGpDALELVelpVPVPGAGQVQIRAeAFGVGQPDALIRRGVYkwm-ppLPANPGNDVAGRISALGPGVEgL 79  Bradyrhizobium ...
YP_887714      1 MKAISVEQFGGpDVLELSevpDPRPAPGEVLISIeASGVGYVDVMAREGHYhaf-teAGFVPGLEIAGVVSELGDGVDaG 79  Mycobacterium s...
YP_001524804   1 MKAVLVYTPGGpDALNYVeapMPLPGAQDVVIRAeAFGVGQPDALIRSGVYkwm-ppLPANPGNDVAGYIETVGRDVTgL 79  Azorhizobium ca...
Feature 1                                                 #         #                    #### ## 
EEH93023      81 APGDYVsvipAFSFHQYGmYGEIVNAPAhAVVKHPQNLsftEAAASWMMYVTAFGALVEYAdikpGDNVLINAASSsVGL 160 Citrobacter sp....
YP_002945770  81 SVGDRVallpGANQGLYPtYGERILFPAsHVVRYPGKLsaeQAAGAYMAYLTGYFPMFEMArlqrGQTVLVTGASSsTGI 160 Variovorax para...
YP_002947569  85 QVGQRVfvsaRELAQRGGcYAEAICVPEaAPFVLPDAIr-fEDAVSLGNFQLALAMLSSNGnl-pAQSVLIPGAAGgVAT 162 Variovorax para...
YP_001262158  80 WLGRRV----FAILRQGGgCAERLTVPAnELIPLPDDIsceNAVAAGLNALVAQVGVARLPit-dTDQVLVRGAGGgIGL 154 Sphingomonas wi...
ACU37962      80 WVGRRV----WAFTGTGGgYAEQAAVPLdDVAELPDGLsavDAVTLGSSATVAHFALAHGRla-sGERVLVRGASGsIGI 154 Actinosynnema m...
YP_003315528  80 WVGRRV----WAFTGTSGgYAEQAVARVdDVVELPDGLspvDAVTVGGASTVARLALTHAHlr-sGESILVRGAAGsIGI 154 Sanguibacter ke...
YP_001106330  81 WVGRRV----WAFTGVGGgYVESAIAPVeEVVPLPANIspaDAVTLGNSGVVAHFGLAHAHfv-pGESVLVRGAAGsIGI 155 Saccharopolyspo...
NP_769719     80 AVGQKVllsaRDLSQRGGcYADYVVAPA-DAVHALPDHvdlQAAVCLSNYQVAYALLHECRhpraPTSVLVIGAAGgVGT 158 Bradyrhizobium ...
YP_887714     80 WLGRRV----FAMPMTGGgYAEYVAVSSdQLIPLPDGVsarSAVALGMNALVAKIAVERMGsa-pGESVYVRGAGGgIGI 154 Mycobacterium s...
YP_001524804  80 EVGQKVllsaRDLSQRGGcYAEYVCAPAnAVHPLADSVs-cEEAVCLANYQVAWALLHEASgprpPQTVLVIGAAGgVGS 158 Azorhizobium ca...
Feature 1                        ##    #              #                       ## #               
EEH93023     161 AAIQIANMRGAKPIAMTRTs-eKRDQLLqLGAAEVIASqeqDLVTEIQRITegkGTRVVFDPVGGPAVAKIAQVMPAgGI 239 Citrobacter sp....
YP_002945770 161 AALQMARAAGLLAIATTRTa-aKRQALRdAGAAHVVVTqdeDVVERVLALTggrGADLVYDGVGGPQLERLGGAVAQrGW 239 Variovorax para...
YP_002947569 163 ALVQVARDRGLRVIGTASTp-qKRDFARaNGAHEVIESdpqGLTEKVMQLTggrGVDLAFDHLGGDLFIACLRALAPfGM 241 Variovorax para...
YP_001262158 155 MCVQYAALRGGTVVATSSSr-eRGERLLaLGASSIWNRldsDLDGPAAFDI---IIDTIIGQDLPAFFDRLGDNAHYlFC 230 Sphingomonas wi...
ACU37962     155 AAVELAAAGGASVVAVTTSspeRGARLReHGATHVLDRa-gRGDGAGEFDV---VVDVVGGADLPGFLDLLAPGGRWvLA 230 Actinosynnema m...
YP_003315528 155 AAVEIAVRAGAAVVAVTTSspeRGDRLRaLGATHVLDR---DGRAESDAPA---LFDVILDIAAGPAVPDFVDRLAPhGR 228 Sanguibacter ke...
YP_001106330 156 TTVQLAARGGADAVAVTTSsaeRGDRLReLGATHVLDR---SGDGGEDAPA---GYDVVIDIIAGADMPAFFDKLNPnGR 229 Saccharopolyspo...
NP_769719    159 ALVQLAKLAQMTVIGTVSTe-aKAEFAKaNGADHIIFYrreDVVARTRELTngeGVGLVLDHVCGPEFTAYLGALGKwGT 237 Bradyrhizobium ...
YP_887714    155 MAVQICNARGAHVTATTSSa-lRGERLRaLGAEHVVDR---HASGESDSRSydlIVDTVAGPQLGSYVAKLKPNGRYiLC 230 Mycobacterium s...
YP_001524804 159 ALLQLARLAGMRTIGSVSTa-eKAEFARaMGADDVVFYraeNVVDRTLELTagaGVDLVLDHVCGADFAGYLRTLRKwGT 237 Azorhizobium ca...
Feature 1           ##                                     ###                                   
EEH93023     240 FFQYGSLdsrdlsIPVIEI--------------LGRHLTFRGYEIfeit--------tdaEKLARAKRFIFEGLq---sG 294 Citrobacter sp....
YP_002945770 240 YVLYGLSgg--aeFSYPVLa------------qFRKSWRFHVYTVleftgsatmglprdeAALGRALAFVDEGLt---sG 302 Variovorax para...
YP_002947569 242 AVSYNIV------NGPPAGdvfee-----lrrlLGRSLAVRTFSIhaid--------adaAQRRGFMEAAIALMa---qG 299 Variovorax para...
YP_001262158 231 GGVGGLPp-----ADFGMKli----------ehFHKSPTVHAFSLnsat--------ldeVAREAAILFEHIAA-----G 282 Sphingomonas wi...
ACU37962     231 GAVAGFPp-----ADFGAAlm----------rgFQRSRSFATFSLatv----------pvAERNRVRARLLAGAv---tG 282 Actinosynnema m...
YP_003315528 229 LVLVGVVagf-ppADFGARll----------ssFQQSRSVATFSLdsv----------paSQRDPVRAAQLDAAa---rG 284 Sanguibacter ke...
YP_001106330 230 MVAVGIVggp-ppAGFGMRmf----------eaFRKSMSFATFSAdt-----------vpEPGRRAVRTEQFAAag--rG 285 Saccharopolyspo...
NP_769719    238 LLSYNAF------AGLPEEnlmvamrnhldicpAVRCFSFHIYDHd-------------rDGRRALMRSVIEALs---rN 295 Bradyrhizobium ...
YP_887714    231 GGVGGAPa-----EDFGRVll----------enFHRSPTFSAFSLn-------------sVENPAIVTTGTELFagadrG 282 Mycobacterium s...
YP_001524804 238 LLSYNAF------AGLPEEnlmgemrkylgncpAVRCFSFHIYDHd-------------rPNRRRIMREVIAALe---aG 295 Azorhizobium ca...
Feature 1                                           
EEH93023     295 QLKPLIDKTFAFDDIVEAHQYMEaNGQVGKIVVTL 329 Citrobacter sp. 30_2
YP_002945770 303 ALQMRIDRRFALDEVVQAHEYLErAGHIGKIVLTV 337 Variovorax paradoxus S110
YP_002947569 300 RVHAPPATCFALADSRRAHEVLDsGSAVGKIVLLP 334 Variovorax paradoxus S110
YP_001262158 283 RISSVVDSVMPMADIVEAHRKLDaGQAFGKIILRP 317 Sphingomonas wittichii RW1
ACU37962     283 EVTPVVHTTLPLAEAAQAHRLMDeGGVFGRIVLLP 317 Actinosynnema mirum DSM 43827
YP_003315528 285 ELHAVVDDVLPLAQAAEAHRRMDrGTVFGRIVLVP 319 Sanguibacter keddieii DSM 10542
YP_001106330 286 ELHTVVHELLPLEQAVLAHQKMDtGEVFGRIVLTP 320 Saccharopolyspora erythraea NRRL 2338
NP_769719    296 AIKPAVSAVLKLDQVRQAHALLEqGAALGKIIMTP 330 Bradyrhizobium japonicum USDA 110
YP_887714    283 AITPVVHCELPLADAASAHAELEaGRVFGKVVLIP 317 Mycobacterium smegmatis str. MC2 155
YP_001524804 296 HIKPAIAARFPLARVRDAHAMLEsGAALGKIIMTP 330 Azorhizobium caulinodans ORS 571

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