Conserved Protein Domain Family
Zn_ADH8

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cd08262: Zn_ADH8 
Alcohol dehydrogenases of the MDR family
The medium chain dehydrogenases/reductase (MDR)/zinc-dependent alcohol dehydrogenase-like family, which contains the zinc-dependent alcohol dehydrogenase (ADH-Zn) and related proteins, is a diverse group of proteins related to the first identified member, class I mammalian ADH. MDRs display a broad range of activities and are distinguished from the smaller short chain dehydrogenases (~ 250 amino acids vs. the ~ 350 amino acids of the MDR). The MDR proteins have 2 domains: a C-terminal NAD(P)-binding Rossmann fold domain of a beta-alpha form and an N-terminal catalytic domain with distant homology to GroES. The MDR group contains a host of activities, including the founding alcohol dehydrogenase (ADH), quinone reductase, sorbitol dehydrogenase, formaldehyde dehydrogenase, butanediol DH, ketose reductase, cinnamyl reductase, and numerous others. The zinc-dependent alcohol dehydrogenases (ADHs) catalyze the NAD(P)(H)-dependent interconversion of alcohols to aldehydes or ketones. Active site zinc has a catalytic role, while structural zinc aids in stability. ADH-like proteins typically form dimers (typically higher plants, mammals) or tetramers (yeast, bacteria), and generally have 2 tightly bound zinc atoms per subunit. The active site zinc is coordinated by a histidine, two cysteines, and a water molecule. The second zinc seems to play a structural role, affects subunit interactions, and is typically coordinated by 4 cysteines.
Statistics
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PSSM-Id: 176223
View PSSM: cd08262
Aligned: 16 rows
Threshold Bit Score: 387.819
Threshold Setting Gi: 39652812
Created: 13-May-2009
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
putative NAD(P)catalytic Zn
Feature 1:putative NAD(P) binding site [chemical binding site]
Evidence:
  • Comment:MDR family binds NAD(P) as a cofactor
  • Comment:defined by comparison to MDR alcohol dehydrogenase like family

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                               ###  #                                   
EEB78787       1 MRAAVFKEvgkpMVVETIADPt--pEANELVVKVGYCGICGTDLHSTregaa-----------------tvpcDSVLGHE 61  marine gamma pr...
YP_385187      1 MRAAVFHQigkpLAIESLNDPi--pLDGEVIVNVKACGICGTDIHATadkem-----------------rladGTVLGHE 61  Geobacter metal...
YP_497148      1 MRAAVMQGlhkpLAIDTIPDPt--pGEGDVVVKVGRCGICGSDLHMTedpay-----------------gqgaGSVLGHE 61  Novosphingobium...
YP_001260795   1 MRAAIFHGagkpMTIEDVPDPe--pGPGEAVIKVCRCGICGSDIGMTsgsha-----------------dypaGTTLGHE 61  Sphingomonas wi...
NP_959378      1 MRAAVLRDgr-mVYRDDVPEPi--pETGQVLVGVRACGICGSDLHFAahgaqvlqmsdrvaggaggmrvdldhDIFMGHE 77  Mycobacterium a...
NP_799894      4 MNSIVMQDg--sVRLKQVEIPk--pAAGQVLVRSLACGICGSDLHITrhadevfdvfhq--lglmpdeagehaQVMLGHE 77  Vibrio parahaem...
YP_759650      1 MRAAVLQGn--sIRIRQMPDPs--pLAGQLLVRPIFTGICGSDLSARkqmaamadtl-------ppeaqgqmpAIIPGHE 69  Hyphomonas nept...
YP_001511381   1 MRAAVLREg--vVEARVIDDPv--pGPGQLLVRSLACGICASDIHFMdhleagvddd------sgmstydrdvDIVMGHE 70  Frankia sp. EAN...
YP_884350      1 MRAVVLRDd--rLQVRETPDPe--pGPGELLLRTLSTAICASDVHFMdhpelalddp------tgrslydsgrDIVLGHE 70  Mycobacterium a...
YP_001714885   1 MKSVLCHQ----ATLQVVDQPnltpAKGQVLLEVVRCGICGSDLHMQhhcdhmhdlaar---vgfnglakstdPFVLGHE 73  Acinetobacter b...
Feature 1                                                                                        
EEB78787      62 FCGEVVETGSAlkskwqIGDRVASLPFvacghcelcargrpfeclqvkltglevpGGFAEYVKTgdaetLKLPDQLSme- 140 marine gamma pr...
YP_385187     62 FAGEIVEVGPQvavgwaQGDRLCTLPYigcghclacltgmpwqcklkkvigiqtaGGFAEYARVhvneaVRLPVSVSwq- 140 Geobacter metal...
YP_497148     62 FAGEVVALGKGveg-lrTGDLVSVIPLqscgqchscrtg----evqwcerfglqgGGYAEFALTrpnqcVRLPASASma- 135 Novosphingobium...
YP_001260795  62 YAGEVVAVGRDvsn-lkVGQRVSAMPAkgcgrcasclag----fplgctamagmiGGFGQYMRIyapaaIHLPDTLSma- 135 Sphingomonas wi...
NP_959378     78 FSAEILEAGPDtet-hpPGTLVTSLPVllsakgvep-----------ivysnttiGGYAERMLLsapllLPIPNGLDvk- 144 Mycobacterium a...
NP_799894     78 FCAEIVEYGSDtqqtlaVGSRVTSVPMllsqngag------------vgvtpgtyGAYSEYFLLdeallLPVPDGMPse- 144 Vibrio parahaem...
YP_759650     70 FSAEIIGIAPGtdtrlkAGDIITGLPFththegpqt-----------iglspahgGGLAELSCIdasrsFRIPDGVPhd- 137 Hyphomonas nept...
YP_001511381  71 YCAEVVDYGPGterripVGARVSSLPVlstatgrki-----------igqnpespGGFGEYLLLd---eAMTRVAVSelp 136 Frankia sp. EAN...
YP_884350     71 FVGEVVGHGPGctgqfaIGTRVTAMPVrlvdggaggm--------riigqhpdaqGSFAELLVVseaaaKPVPGEVSsd- 141 Mycobacterium a...
YP_001714885  74 FCGKVLDYGPKtrhkfkADTLVCAMPLlnvdqhgyl----------stglspnasGGYAEQVLAqsslmFEVPNGLDad- 142 Acinetobacter b...
Feature 1               #   #                   ######                  ##   #              #    
EEB78787     141 --tAALIEPLAVGLHAVRVSrlaaGSRVLIIGGGPVGLAVALWARFMGArdVLVSEMAptRIALASRL-GATGTLMPget 217 marine gamma pr...
YP_385187    141 --eGALVEPLAIGLHAVRLSrgvaGKSVLVTGAGPIGLAVALWCRFFGArqVVVSEFDpeRSKMALAM-GATHAVDAkad 217 Geobacter metal...
YP_497148    136 --dGAIVEPLAVALHGLALSrmkiGDKVLVLGAGPIGLAVAFWARRFGAgrVVVQDLAewQRDRALQM-GAHDFVVDaad 212 Novosphingobium...
YP_001260795 136 --dGALVEPLAVGLRGVALAglrpGATVAVLGAGAVGLAAIYWARLLGAgrIVAMSRSarRADLATAM-GADGFEAFgeg 212 Sphingomonas wi...
NP_959378    145 --hAALTEPMAVGLHAVNKSnitpAETALVLGCGPIGIAIIAALATRGVesIVASDFSpkRRQLATAM-GAHRTLDPaqg 221 Mycobacterium a...
NP_799894    145 --aAALTEPLAVGLHAVNRSeietEETALVVGCGPIGLAVIAALKQRGVsnILAVDLQeeKLQLASEF-GANRLISPtke 221 Vibrio parahaem...
YP_759650    138 --lAALTEPLAVGLHAANLAsr-nRAPNVVIGCGPVGLAVILSLSLQGRgpILAADFSaeRRHAASLL-GADIVIDPrad 213 Hyphomonas nept...
YP_001511381 137 neiVCIADAVSVGLSAASRAqvtaKEVPLVIGCGAIALSVIAQLKRLGVgpILAVDFVasRRETALAM-GADVVIDPaav 215 Frankia sp. EAN...
YP_884350    142 --aVAVTDAFAVGEFYVRSArlqpGEVAIVIGAGAIGLSAVAALAARGVepIIVADFRadRRRLAADSfGAHLLVDPree 219 Mycobacterium a...
YP_001714885 143 --sAAMTEPMAVALHAVRRSrvktSEPAIVIGCGPVGLGVILMLKAAGVktVVASDFSpnRRKLAEQC-GADIVVDPket 219 Acinetobacter b...
Feature 1                                                                 ## #                   
EEB78787     218 lgeqf---------------------------------------adtsgaaPDIIFECVGAPGLIQQCIELAPrRGQVIP 258 marine gamma pr...
YP_385187    218 vgaqy---------------------------------------qeitgelPELIFECVGIPGLIAQCVDRAAfGAEIVV 258 Geobacter metal...
YP_497148    213 pvgsa---------------------------------------gralggkADIVFECVGVPGLIAQAVEQVRnDGTITL 253 Novosphingobium...
YP_001260795 213 egdrl---------------------------------------aaalgglPDAVLECAGAVGLMQKAVELVRpGGTIVS 253 Sphingomonas wi...
NP_959378    222 sp--------------------------------------------fdsvkPAVVFEAVGVPGIIDDVLLRARpGTRLVV 257 Mycobacterium a...
NP_799894    222 deiav--------------------------------------geqyamnsRLVIYECVGKHTLIDDFLRRAPaRARIVI 263 Vibrio parahaem...
YP_759650    214 safthwgdlglqp----------------------fpmspllerdfrglppGANIFECTGAQGLIDQVIKGAPpHSHIIV 271 Hyphomonas nept...
YP_001511381 216 spyqawrdvayg------------------------speamrelmavaglpGCVVFECVGIPGVLDSIIKGCErNTRIFS 271 Frankia sp. EAN...
YP_884350    220 spfaafn----------------------------------aararygipgPAAVFECVGAPGLIQNLVESAEmGTRIYC 265 Mycobacterium a...
YP_001714885 220 spfanwkefgllgnvsdainmgmglfdklqatrlpwwhgwrmidklgalpkRPVIFECVGVPGVLQQIIEGAPlFSRIVG 299 Acinetobacter b...
Feature 1        ##                        ###                                                   
EEB78787     259 VGVc--EQQDNFMPFLAlvKELSMQFAIAYTRddfETVIAMLAQGRIDpsaMVTDVVSLDDMPAAFEALRNPSHqCKVLT 336 marine gamma pr...
YP_385187    259 VGFc--MRPDTFVPALAmlKEITVKFSIGNNKsdfQFIVDMLSSGRISvkpMITAAVSLDDLPKTFESLRTPGEhCKVVV 336 Geobacter metal...
YP_497148    254 LGLctrPDTFNSFAMLS--KQVKLVTSAFFTRqeyEAALDALVRGAVEprlLVTDTISLDATPDVFESLRKRTHqCKVLI 331 Novosphingobium...
YP_001260795 254 LGFc--GSPDPIIPSLAtwKQVTMKFSFAYDLrefQHCADALDAGHVAprmMVSETIGLDALPSGFEALRTGSTqTKVHV 331 Sphingomonas wi...
NP_959378    258 AGVc--MQPDTVHPFFAiaKEINVQFVLAYTPeefGDSLRALAEGDIDvapLITGEVGLDGVGAAFDDLADPERhCKVLV 335 Mycobacterium a...
NP_799894    264 SGIhttPAIINYAYATV--KELDLRFSYYYQPhefEQCLQAIAEHKLPwqkMLTAKVGIDGVEDAFRQLMTPNDhIKVVI 341 Vibrio parahaem...
YP_759650    272 AGVc--PHEEKLTPLDGilRELTLEFSFAYRPeefAAALAMIEAHPDKaarLITSRQPLANTEAAFDSLASSPSeIKILI 349 Hyphomonas nept...
YP_001511381 272 VGGp--PEGDHLHTLTAkrKGINIQFGGGPSMqhwDEAFAAVGSGDLDvtpMLGRTVGLDDVAEALNASRDANGpVRIVV 349 Frankia sp. EAN...
YP_884350    266 AGGwytGDTLDITTATR--QGVTIQFGGGPHPqdwYGTLDAIVAGRLDplpSVGKIIGLDEVPDALELARRSDGpPRIIV 343 Mycobacterium a...
YP_001714885 300 VGVc--MQSDKIEPALAinKELEIQFVLGYTPlefRDALHMIAEGKVNcspLITGVVGLEGVTNAFEALRDPEQhAKILI 377 Acinetobacter b...
Feature 1         
EEB78787     337 R 337 marine gamma proteobacterium HTCC2148
YP_385187    337 E 337 Geobacter metallireducens GS-15
YP_497148    332 N 332 Novosphingobium aromaticivorans DSM 12444
YP_001260795 332 D 332 Sphingomonas wittichii RW1
NP_959378    336 T 336 Mycobacterium avium subsp. paratuberculosis K-10
NP_799894    342 E 342 Vibrio parahaemolyticus RIMD 2210633
YP_759650    350 D 350 Hyphomonas neptunium ATCC 15444
YP_001511381 350 V 350 Frankia sp. EAN1pec
YP_884350    344 H 344 Mycobacterium avium 104
YP_001714885 378 D 378 Acinetobacter baumannii AYE

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