Conserved Protein Domain Family
Zn_ADH7

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cd08261: Zn_ADH7 
Alcohol dehydrogenases of the MDR family
This group contains members identified as related to zinc-dependent alcohol dehydrogenase and other members of the MDR family. The medium chain dehydrogenases/reductase (MDR)/zinc-dependent alcohol dehydrogenase-like family, which contains the zinc-dependent alcohol dehydrogenase (ADH-Zn) and related proteins, is a diverse group of proteins related to the first identified member, class I mammalian ADH. MDRs display a broad range of activities and are distinguished from the smaller short chain dehydrogenases (~ 250 amino acids vs. the ~ 350 amino acids of the MDR). The MDR proteins have 2 domains: a C-terminal NAD(P)-binding Rossmann fold domain of a beta-alpha form and an N-terminal catalytic domain with distant homology to GroES. The MDR group includes various activities, including the founding alcohol dehydrogenase (ADH), quinone reductase, sorbitol dehydrogenase, formaldehyde dehydrogenase, butanediol DH, ketose reductase, cinnamyl reductase, and numerous others. The zinc-dependent alcohol dehydrogenases (ADHs) catalyze the NAD(P)(H)-dependent interconversion of alcohols to aldehydes or ketones. Active site zinc has a catalytic role, while structural zinc aids in stability. ADH-like proteins typically form dimers (typically higher plants, mammals) or tetramers (yeast, bacteria), and generally have 2 tightly bound zinc atoms per subunit. The active site zinc is coordinated by a histidine, two cysteines, and a water molecule. The second zinc seems to play a structural role, affects subunit interactions, and is typically coordinated by 4 cysteines.
Statistics
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PSSM-Id: 176222
View PSSM: cd08261
Aligned: 40 rows
Threshold Bit Score: 370.751
Threshold Setting Gi: 118470517
Created: 4-Feb-2009
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
Feature 1:putative NAD(P) binding site [chemical binding site]
Evidence:
  • Comment:MDR family binds NAD(P) as a cofactor
  • Comment:defined by comparison to MDR alcohol dehydrogenase like family

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                               ###  #                                   
P38105         1 MKSILIEkpnqlAIVEREIPTPs---aGEVRVKVKLAGICGSDSHIYRGhNPFAKYPRVIGHEFFGVIDAVGegveSARV 77  Escherichia col...
YP_174130      1 MRAIEVRkp--gDIHVVEVQKPeletdKDVLVKVQAVGICGSDMHIYHGsNPFTVYPRIIGHEVGGIVEEVGsgvhGLRR 78  Bacillus clausi...
NP_389115      1 MKAVQVRka--yDLVTAEVKKPvlskdDEVLVKVKRVGICGSDMHIYHGtNPLATLPRVIGHEVTGQVEAVGanvqSLKP 78  Bacillus subtil...
YP_001338916   1 MKAFQVAnv--nDYALIDTLQPva-eaGEVLIKTAFAGICGSDLHIIHGqNPFVQFPRITGHEFSGVVAAVGegvtHVKI 77  Marinomonas sp....
YP_575019      1 MKAFEVRap--hDFGIVDIDAPqa-aaGEVLVGVAFAGICGSDMHIIHGeNAFVRFPRITGHEFAGIVEDIGdgveGLAV 77  Chromohalobacte...
YP_001343682   1 MKAITVSqp--fELTVADVPAPtvtkdDDVIIKVAFGGICGSDIGIYTGgNSLAKYPAIIGHEFVGTVVETGkavtHVKV 78  Actinobacillus ...
ACM30051       1 MVAIAVRap--hRLALEDENSPvdlapGEVSIRVVRAGICGSDIHILHGsNPFVVYPRIIGHEFAGTVEAIGagvsRLAI 78  Agrobacterium r...
YP_078544      1 MKAAVMTkp--ySIEFHDIPRPep-asGEVLVKIKAAGICGSDVHFYDGsNPYAQYPQIFGHELAGIIEKTGagvrGRSA 77  Bacillus lichen...
YP_003323734   1 MQAVIMDaprqaRVGQWEARQPg---eGEVLVRVRSVGVCAGDMYFYLGkNPYAVYPQVCGHEISGVVESLGegvrGLSA 77  Thermobaculum t...
YP_003153356   1 MKAVRINkv--eYLEIVDLEEIqieedDDIKIKVKSVGICGSDIGIYNGtNPMAEYPRIIGHEMTGVVEEVGnkvkGLKV 78  Anaerococcus pr...
Feature 1                                                                           #   #        
P38105        78 GERVAVDPVVsCGHCYPCSiGKPNVCTTLaVLGVHADGGFSEYAVVPAk-nAWKIPEAVAdqyAVMIEPFTIAANVTGHG 156 Escherichia col...
YP_174130     79 GDRVALEPISsCGVCYACKkGRPNVCAKLeVFGVHRDGGMREWMVAPEa-nWHKVNGDLPfeaAALVEPMTIGAQAAYRG 157 Bacillus clausi...
NP_389115     79 GDHVVIEPISyCGSCYACRkGRPNVCAKLsVFGVHEDGGMREYIVLPEr-qLHAVSKDLPweeAVMAEPYTIGAQAVWRG 157 Bacillus subtil...
YP_001338916  78 GDKVCVDPVIsCGECYACRaGRFNVCAKLqVFGVHRNGGFGEFTSAPAs-nVLVLPDNVTleqAALVEPYSIATNVLSRM 156 Marinomonas sp....
YP_575019     78 CDRVCIDPVIsCGECYPCRiGRPNVCTRLaVIGVHRNGGFEQRVSVPAd-nVHRLPANVGldqAALVEPYTIAANVLERM 156 Chromohalobacte...
YP_001343682  79 GDYVVSDIVNsCGHCYACRtGHHNVCKNLqVTGVHSQGGFAEYAKTRAdnvYLVDTNKIPhktACLIEPYAVGVEINTRA 158 Actinobacillus ...
ACM30051      79 GNRVVADPVIsCGKCHPCRiGRSNVCANLqVIGVHRDGGFRSVAVVPEa-nAVKVSPALGldiAALAEPLAVAANVLSRT 157 Agrobacterium r...
YP_078544     78 GERVVIEPAIpCGGCYPCRkGRTNACMNIdMIGSVRRGGFADFIIVPEt-hVHPIPEQMDfatAALCEPFSIGAQAVRRA 156 Bacillus lichen...
YP_003323734  78 GDHVVVEPFIgCGRCYACRiGKRNCCRRLqIIGIHRPGGFAEYVLAPAq-nVHKIPESLPferASLSEPIAIAVQASRRG 156 Thermobaculum t...
YP_003153356  79 GDHVAVDPVInCGECKNCKkGRVNICENLkVRGVHVDGGFQEKIVVKEs-qAYKLDPSMPwnkSVLVEPFSIGFQANRRA 157 Anaerococcus pr...
Feature 1                   ######                   ##   #              #                       
P38105       157 qptENDTVLVYGAGPIGLTIVQVLKGVYNvknVIVADRidERLEKAKEsgaDWAINNsq----tpLGEIFTEkgi--kpT 230 Escherichia col...
YP_174130    158 niePGDTVFIMGAGPTGIACLIMAKQKGAt--VFISDLqaNRLDYAKQvgaDFTIHLphekpeavLFEKTDGef----aN 231 Bacillus clausi...
NP_389115    158 qveKGDTVLIQGAGPIGICVLKMAKLAGAa--VMMTDLnnERLAFAKEngaDAVVNVqaehvaerVLEWTGNeg----aN 231 Bacillus subtil...
YP_001338916 157 epiPGDTLLVYGAGVIGLTIVQVAKAMGIe-rIIVTDIvdERLETAKSlgaTDVINSrehkvedcIREWTNGeg----vP 231 Marinomonas sp....
YP_575019    157 qphDGDALLILGAGVIGLTILQVARAKGLt-dITVCDVidDRLDAARSlgaSHTVNGqrqdleavMSERSDGeg----iP 231 Chromohalobacte...
YP_001343682 159 siaAGDKVVVFGSGPAGLAVMQVARARGAe--VLITDIfeERLQLAREmgaTKAINVkdidmrqaVNEFTDDeg----aH 232 Actinobacillus ...
ACM30051     158 gcgPEDVVLIYGAGTVGLTVLQVAKMKGAr--CIVADIdnQRLERAREfgaDVAFNSle----tsVSDMVAGeldglgpS 231 Agrobacterium r...
YP_078544    157 diqTGETIVILGMGPIGLTILAQVKKRFDv-rVIAVDPvrERLELAEAfgaDAVIQPgeedaeqmILKLTGGeg----aG 231 Bacillus lichen...
YP_003323734 157 qitAEDQVLVMGCGPIGLALIDVLSAKGAq--VFATDInpARLDAASQl-gARPLPGgeel-psrVSELTEGdg----mP 228 Thermobaculum t...
YP_003153356 158 nvsEGDAVFVMGAGSIGQTVGRVAQYLGAk--VFTSDMdpKKLERASKy-gMIAIDAskedpaevIRENNNGrl----aD 230 Anaerococcus pr...
Feature 1            ## #                   ##                        ###                        
P38105       231 LIIDAACHpsILKEAVTLASPAARIVLMGFssePSEVIQQg--ITGKELSIFSSRLNAnKFPIVIDWLSKG-LIKpekLI 307 Escherichia col...
YP_174130    232 VVIDAVGTstTFAEAVKLASVAGTVVTLGFnetPSDIPSLl--LTKKELTVAGSRLQTnQFPDVIRQVNEQ-KVDpasII 308 Bacillus clausi...
NP_389115    232 VVIDAVCLpeTFALSIEAVSPAGHVVVLGFderAAQISQLp--ITKKEVTITGSRLQTnQFPKVVELLNGG-RLMhngLV 308 Bacillus subtil...
YP_001338916 232 LIADAACIpaLLPEILRIACPAGRIGLLGFsttPSDLAQIe--VIKKELTIVGSRLNNrRFPQVLEMIASG-RLDplaLI 308 Marinomonas sp....
YP_575019    232 LIADAACVpaLFPQLARIASPAGRIGLLGFnatPSDLVQLe--VIKKELTIVGSRLNNrKFPDVIEMLASG-RLDasaLV 308 Chromohalobacte...
YP_001343682 233 VVVDAVCSpqSILDALDIVAPSGRLVVLGTgaaPSEIPQVa--FTKKGINVFGTRLNNkRFPEVIRLFEEH-KVTpekMI 309 Actinobacillus ...
ACM30051     232 VVIDGAGIpaLLEEACRVAAPAGRIGLLGFsaaPCNVSQQe--IVRKELSLVGSRLNRrFIPEVVGWLESG-ALKpaaMI 308 Agrobacterium r...
YP_078544    232 IVFEAAGIpaTIEQSVRLAAAAGRIVIVGLtgrNVTIPGLl--LTKKDIEIYGTKHSVnQFPGVIQFLNENpEIAr-aFV 308 Bacillus lichen...
YP_003323734 229 VVIEATGNpqAMRQAVDLVAPGGRVVIVGLvpqGVDVPMPgldFTRKEMTILGSRASVnCFPEAIELLAGG-KIRy-aDL 306 Thermobaculum t...
YP_003153356 231 VVVDAICTpkTFEQAVKLAASAGTVVNLGFstkPSEICSVd--ITSKELDIVGSRLSNrRFPEVIDAYNSG-KLSfddLV 307 Anaerococcus pr...
Feature 1                                      
P38105       308 THTFDFqHVADAISLFEQDqkhCCKVLLTF 337 Escherichia coli K-12
YP_174130    309 SHRFDFdEVKKAIELLEKEpahVRKAVLIF 338 Bacillus clausii KSM-K16
NP_389115    309 THTFSVdDVHHAFQFIKEHpdqVRKAVITF 338 Bacillus subtilis subsp. subtilis str. 168
YP_001338916 309 SHRIALnDIGDGIQLMEKHpelTRKVLVEM 338 Marinomonas sp. MWYL1
YP_575019    309 SHRLPFaEMGEAIHMIDHHperVRKVLIEL 338 Chromohalobacter salexigens DSM 3043
YP_001343682 310 THTYKFeDIEKAFDVIKNEkntVCKVLLEW 339 Actinobacillus succinogenes 130Z
ACM30051     309 TQTFAAaDAAAAFDLIERHpekTLKVQLAF 338 Agrobacterium radiobacter K84
YP_078544    309 TEIMPFmEIEEALKKAKNCphqVTKIILSY 338 Bacillus licheniformis ATCC 14580
YP_003323734 307 ATTFSLwDAPALFADWAQHpgsVQKAILRV 336 Thermobaculum terrenum ATCC BAA-798
YP_003153356 308 SHEMKFeEAEKAIKMIMDKdiyTEKVILIL 337 Anaerococcus prevotii DSM 20548

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