Conserved Protein Domain Family
Zn_ADH4

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cd08258: Zn_ADH4 
Alcohol dehydrogenases of the MDR family
This group shares the zinc coordination sites of the zinc-dependent alcohol dehydrogenases. The medium chain dehydrogenases/reductase (MDR)/zinc-dependent alcohol dehydrogenase-like family, which contains the zinc-dependent alcohol dehydrogenase (ADH-Zn) and related proteins, is a diverse group of proteins related to the first identified member, class I mammalian ADH. MDRs display a broad range of activities and are distinguished from the smaller short chain dehydrogenases (~ 250 amino acids vs. the ~ 350 amino acids of the MDR). The MDR proteins have 2 domains: a C-terminal NAD(P)-binding Rossmann fold domain of an beta-alpha form and an N-terminal catalytic domain with distant homology to GroES. The MDR group contains a host of activities, including the founding alcohol dehydrogenase (ADH), quinone reductase, sorbitol dehydrogenase, formaldehyde dehydrogenase, butanediol DH, ketose reductase, cinnamyl reductase, and numerous others. The zinc-dependent alcohol dehydrogenases (ADHs) catalyze the NAD(P)(H)-dependent interconversion of alcohols to aldehydes or ketones. Active site zinc has a catalytic role, while structural zinc aids in stability. ADH-like proteins typically form dimers (typically higher plants, mammals) or tetramers (yeast, bacteria), and generally have 2 tightly bound zinc atoms per subunit. The active site zinc is coordinated by a histidine, two cysteines, and a water molecule. The second zinc seems to play a structural role, affects subunit interactions, and is typically coordinated by 4 cysteines.
Statistics
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PSSM-Id: 176219
View PSSM: cd08258
Aligned: 14 rows
Threshold Bit Score: 327.735
Threshold Setting Gi: 116670346
Created: 13-May-2009
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
Feature 1:putative NAD(P) binding site [chemical binding site]
Evidence:
  • Comment:MDR family binds NAD(P) as a cofactor
  • Comment:defined by comparison to MDR alcohol dehydrogenase like family

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                              ###  #                                    
NP_466185      1 MKAVVKTnpgydQMELKDVEEPqvygDKVKIKVAFTGICGSDIHtfkgey--knpttPVTLGHEFSGVVVEVGpdvt-si 77  Listeria monocy...
Q9Z9U1         1 MKALVKTqhgtgHFAVQEKPEPtpgkHQVKIKVKYTGVCGSDIHtyegh---ypvaaPVTLGHEFSGEIVELGegvt-gf 76  Bacillus halodu...
P77539         1 MKALARFgkafgGYKMIDVPQPmcgpEDVVIEIKAAAICGADMKhynvds--gsdefNSIRGHEFAGCIAQVGekvk-dw 77  Escherichia col...
NP_866632     57 SAAVVNYapepgSVEIREIDRPeigsQDVLLEVSHVGVCGSDLHqwtahh-swpvnyPVVLGHEFGGHIVQLGsdve-gw 134 Pirellula sp. 1
EDY40157       1 MKALVKYargpwNIELRDVPEPksreGYIIVDVKAAGICASDLLiihdkg--hpyepPVILGHEFSGVIAEDKge----w 74  Thermococcus ba...
YP_001210822   1 MRAIVKEerrpgAVNLRSVPEPrpagDEVLIRVQSAAVCGSDLHayeypksyefmkvPVILGHEYSGYVEAVGpqvt-lf 79  Pelotomaculum t...
YP_003309993   1 MKALARYgkefgGYKLIDIPKPecgpDDIIVEIKAAAICGADMKhykvdn--gsdefNSVRGHEFAGEIVEIGknvv-dw 77  Sebaldella term...
YP_003323993   1 MRALVQYglrpgEVELRDIPVPevteDSVLLKVDAVGICGSDIHqvhgtq-swevcvPVVLGHEFTGTIERVGpnvv-gf 78  Thermobaculum t...
YP_003202642   1 MRGLVKFdsgpgNVELRQVPAPelgaGQILIDVAAVAICGTDRLaieggh--dhgklPRVLGHEVSGTISAIGpdvesdl 78  Nakamurella mul...
YP_002884187   1 MHAVRKTgpepgDVELQEVPRPtagpGQVVVDVAAAGLCGTDLHildgs---ygsrpPVTLGHEVSGTIAAVGpgvd-aa 76  Beutenbergia ca...
Feature 1                                                                                       #
NP_466185     78 kVGDRVTSettfetCGECIYCkeHDYNLCSnr---rgIGTQANGSFAEFVLSREes-------cHVLDErisleaAALTE 147 Listeria monocy...
Q9Z9U1        77 nVGDRVTSettysiCGKCSYCtsGDYNLCShr---kgLGNQQDGSFAKYVIARQes-------lHHLPAgvddrsAAMTE 146 Bacillus halodu...
P77539        78 kVGQRVVSdnsghvCGVCPACeqGDFLCCTekvnlglDNNTWGGGFSKYCLVPGeilkihrhalWEIPDgvdyedAAVLD 157 Escherichia col...
NP_866632    135 kEGDRVVSetaaiiDSNNPMSrrGLYNLDPtr---kgFGYGVNGAMTRYVRVPSri-------lHEVPHslafehACLTE 204 Pirellula sp. 1
EDY40157      75 kKGDRVTSqttfstCGKCYLCksGYPQHCPek---kvIGIKANGAFTEKILVPVsg-------lHRIPKnmsfeeAAITE 144 Thermococcus ba...
YP_001210822  80 kPGDRVLGesn-ryCGVCPNCrrGRTNICDsn---lmTGLHVDGGMAEFIAVPQkl-------vHHIPEnlsfdeATLAQ 148 Pelotomaculum t...
YP_003309993  78 kIGQRVVSdnsghvCGVCPACeqGDFLCCTekvnlglDNNRWGGGFSKYCLIPGeilkihkhaiWEIPEnlkyeeAAVLD 157 Sebaldella term...
YP_003323993  79 rPGQRVVSetaaviCGRCTPCrtGHYNLCPhr---qgFGYGIDGAMAEYVRVPArc-------lHAIPQdsdavaMALTE 148 Thermobaculum t...
YP_003202642  79 qVGDRVTVetdaylCGRCAYCrrEEYNRCPyr---lgIGTTADGGLADQLAMPAla-------vHKLPDnvsllaGALTE 148 Nakamurella mul...
YP_002884187  77 rVGERVALetffstCGECRDCrgGSPNRCEsr---vsIGSGADGGFAEQVVVPArn-------aRRLPDhvplpaGALSE 146 Beutenbergia ca...
Feature 1           #                     ######                  ##       #                     
NP_466185    148 PLACCVHSALEktt-irpDDTVLVFGPGPIGLLLAQVVKAQGAt-VIMAGItkd--sdRLRLAKELGmd-----riVDTL 218 Listeria monocy...
Q9Z9U1       147 PLACTHHAIAKtsi--nkGDLVVVTGPGPIGLLAAQVAKSHGGt-VIITGLsnd--qvRLKKAKEVGidyaidtqeVDIK 221 Bacillus halodu...
P77539       158 PICNAYKSIAQqsk-flpGQDVVVIGTGPLGLFSVQMARIMGAvnIVVVGLqed-vavRFPVAKELGatavvngstEDVV 235 Escherichia col...
NP_866632    205 PCCVAYNAVVRnar-iepGDRVIVMGPGTIGILCAAMARLCGAe-VALVGLesd--rhRLNIAEENYgcq---givGDPT 277 Pirellula sp. 1
EDY40157     145 IGADVVYALNEran-lqpGEFVVIFGPGAVGLFAVQVAKATGAe-VAIVGTkierdeyRLKLAEKLGadhv-lyaeEDPV 221 Thermococcus ba...
YP_001210822 149 PCSVSFHAVFDnsg-irpNDMVAVFGPGIVGLMAAQGARLLGAaeVVVIGTged-aqnRLPVAQKLGfkti-nigeQDLL 225 Pelotomaculum t...
YP_003309993 158 PICNAYKSIAQqsk-flpGQDVVVFGTGPLGLFSVQMARIMGAvnIVVVGLedd-akvRFDIAKELGatdvvnasrEDVV 235 Sebaldella term...
YP_003323993 149 PACVAYQAVVQsar-vraGDSVLVLGPGQIGLLSAMLCKLQGAqpVVVMGTerd--ehRLRKAEELGvdaalrvdqADLR 225 Thermobaculum t...
YP_003202642 149 PLAVSVHAVIEqsp-vlaGEVVVVIGPGAIGQLCAQVALAAGAt-VVMVGRsrh--teQLALARRMGvthtvdsetIDVA 224 Nakamurella mul...
YP_002884187 147 PLACVCRSLFHptpavraGDRVLVVGPGAIGLLAAQVARVCGGe-PTVLGTpad--aeRLDVARALGla-----caDDPA 218 Beutenbergia ca...
Feature 1         #                    ## #                   ##                          ###    
NP_466185    219 KEDLAEvvlgmtggygaerVFDCSGAvpaVNQGLPLTKkKGDFVQVGLfa-ekKNAIDEesii--qreIAYIGSRSqkps 295 Listeria monocy...
Q9Z9U1       222 ELVSELtdgy-----gadvVLECSGAvpaAKQGIDLLRkKGQYAQVGLf---aQPEIQFnfekiiqkeISVVGSRSqkpa 293 Bacillus halodu...
P77539       236 ARCQQIcgkd-----nlglVIECSGAniaLKQAIEMLRpNGEVVRVGMgfkplDFSINDita----wnKSIIGHMAydst 306 Escherichia col...
NP_866632    278 DWANQRdglg------cdgVIDAAGSsvtLEIAMQVVRpAGWISKVGWgpqplGYNLDPlvq----knVTLQGSFShnwp 347 Pirellula sp. 1
EDY40157     222 SEVKYLtngk-----gadiAFEASGAapaVLQALQITRnLGRIIAFGIp----KGSVTVpwnmivfkaVEVIFHLSsswt 292 Thermococcus ba...
YP_001210822 226 AGFAGItgke-----radvVLECSGAvkaLADAFRLVKkGGSITLVGIyg--sGAEINFaplv--rgeIQIKTSYTatwe 296 Pelotomaculum t...
YP_003309993 236 KRCQEIcgkd-----nlglVIECSGAniaLKQSIEMLRpNGEVVRVGMgfkplEFSINDits----wnKSIIGHMAydst 306 Sebaldella term...
YP_003323993 226 ELASGIgdgl-----gfhlVVDASGAsasFVTAMEAVRpLGEIVKVGWgpqplAASLDPlvr----kaVTVRGSFShtye 296 Thermobaculum t...
YP_003202642 225 DYVKGLtdgy-----gahtVFECSGGagvLEGVMPWLRrGGRIVLLAFfr--tPPAVDVdrll--nheLELVGSRGkras 295 Nakamurella mul...
YP_002884187 219 DAPSEVd-----------vVLECSGSgagMALGIARVRrGGRYVQIGQrg--dAVPIDLarvs--fheLVITGGFAstpe 283 Beutenbergia ca...
Feature 1                    
NP_466185    296 sWILALDLLANG 307 Listeria monocytogenes EGD-e
Q9Z9U1       294 dWEPALSLLNEK 305 Bacillus halodurans
P77539       307 sWRNAIRLLASG 318 Escherichia coli K-12
NP_866632    348 iWERVLALLTSG 359 Pirellula sp. 1
EDY40157     293 sWEKILYLMREG 304 Thermococcus barophilus MP
YP_001210822 297 nYEQSLSLISRG 308 Pelotomaculum thermopropionicum SI
YP_003309993 307 sWRNALRLLESG 318 Sebaldella termitidis ATCC 33386
YP_003323993 297 tWEQVIRLIASG 308 Thermobaculum terrenum ATCC BAA-798
YP_003202642 296 sYRTALRLMAQG 307 Nakamurella multipartita DSM 44233
YP_002884187 284 sWDRAMLLLERG 295 Beutenbergia cavernae DSM 12333

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