Conserved Protein Domain Family
Zn_ADH2

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cd08256: Zn_ADH2 
Alcohol dehydrogenases of the MDR family
This group has the characteristic catalytic and structural zinc-binding sites of the zinc-dependent alcohol dehydrogenases of the MDR family. The medium chain dehydrogenases/reductase (MDR)/zinc-dependent alcohol dehydrogenase-like family, which contains the zinc-dependent alcohol dehydrogenase (ADH-Zn) and related proteins, is a diverse group of proteins related to the first identified member, class I mammalian ADH. MDRs display a broad range of activities and are distinguished from the smaller short chain dehydrogenases (~ 250 amino acids vs. the ~ 350 amino acids of the MDR). The MDR proteins have 2 domains: a C-terminal NAD(P)-binding Rossmann fold domain of a beta-alpha form and an N-terminal catalytic domain with distant homology to GroES. The MDR group contains a host of activities, including the founding alcohol dehydrogenase (ADH), quinone reductase, sorbitol dehydrogenase, formaldehyde dehydrogenase, butanediol DH, ketose reductase, cinnamyl reductase, and numerous others. The zinc-dependent alcohol dehydrogenases (ADHs) catalyze the NAD(P)(H)-dependent interconversion of alcohols to aldehydes or ketones. Active site zinc has a catalytic role, while structural zinc aids in stability.
Statistics
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PSSM-Id: 176218
View PSSM: cd08256
Aligned: 10 rows
Threshold Bit Score: 650.24
Threshold Setting Gi: 187925206
Created: 28-Sep-2009
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
Feature 1:putative NAD(P) binding site [chemical binding site]
Evidence:
  • Comment:MDR family binds NAD(P) as a cofactor
  • Comment:defined by comparison to MDR alcohol dehydrogenase like family

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                             ###  #                                     
YP_002508080   8 MKALVAYAPGDYRFEEVDVPr-AGEGEIVVKVEACGICAGDIKaYHGAPSFWGGegnppYIKAPMIPGHEFIGEIVEKGA 86  Halothermothrix...
YP_001896848  13 MTAIVCHAPKDYRVEQVSKPr-AGAHELVIRIAACGICASDCKcHSGAKMFWGGps--pWVKAPVIPGHEFFGFVEEIGE 89  Burkholderia ph...
NP_668172     30 MRAVVAYGPRDYRFERVPVPt-IDAKEILVKVEGCGICAGDTKaFEGAPSFWGDdkqpaYIKAPMIPGHEFIGHVVGLGA 108 Yersinia pestis...
NP_993646     48 MSAVVCHGPQDYRLEHVPTPv-PKAKELVIKVKGCGICAGDCKcKNGAKMFWGEt---pWVKPPVVPGHEFYGHIVALGE 123 Yersinia pestis...
YP_001361838  33 MQAVIVHGPHDYRLEEVPVPv-AGPGEALLKVEAVGICASDLKcYHGAAKFWGDenrpaWAETDTVPGHEFTGRIAEIDE 111 Kineococcus rad...
YP_300614     11 MNAVVAYAPGEYKYETVETPtiEHPKEIVVKVEACGICAGDIKaYGGAPSFWGDekqpsYIKAPMIPGHEFIARIVEKGE 90  Staphylococcus ...
YP_106361     25 MRAVVCHGPQDYRLEQVPVPk-PGPDEILTQVERVGICMGDIKtFRGAPSFWGDavqprYVKPPMIPGHEFVCRVVALGP 103 Burkholderia ma...
YP_274915      1 MQAVVCYGPEDYRLETVDVPt-PGPDEILTKVELCGICMGDIKtYRGAPSFWGDaeqprYVKPPMIPGHEFVCRVVALGP 79  Pseudomonas syr...
YP_003013071  11 MKALVAYGKDDYRFEPSFPTpiCGPDDIIIKTEACGVCASDLKcLHGAAMFWGDenqapWVKTPFIPGHEFLGTVVQVGG 90  Paenibacillus s...
YP_003323781   9 MRAVVCHGPEDYRLEELPVPr-PGPGEVLVRVPAVGICASDLKcYTGAPMFWGDehrvgYCQPPVVPGHEFVGYVVALGE 87  Thermobaculum t...
Feature 1                                                                                        
YP_002508080  87 EVsd--dFEIGDRVISEQIVPCWECKFCRTGRYWMCQKHDVYGFQnn-vnGGMAEYMKFPKEAINYKVPKdipiEKAILI 163 Halothermothrix...
YP_001896848  90 GAadhfgVKMGDRVIAEQIVPCGKCRYCKSGQYWMCEVHNIFGFQrevadGGMAEYMRIPPTAIVHKIPDgislEDAAII 169 Burkholderia ph...
NP_668172    109 DVe---gFELGDRVTSEQIVPCWGCRFCNHGQYWMCEKHDLYGFQsn-vnGAMAEYLKFTKEAINYHVPDhlplEKAILI 184 Yersinia pestis...
NP_993646    124 GTes--kYKLGERVIAEQIVPCWDCRYCNSGSYWMCETHNIYGFQkdvaeGGMAEYMRFSENAIVHKIPEslshEDAVLI 201 Yersinia pestis...
YP_001361838 112 EAserwgVVVGDRIVAEQIVPCWNCRYCNRGDYHMCQPHDMFGFKrr-tpGAMAEYLVLPKEALVHKAPDdl-pPWQVAY 189 Kineococcus rad...
YP_300614     91 DVt---dFEIGDRVISEQIVPCWNCRFCNRGEYWMCEKHDLYGFQnn-vnGGMAEYMKFTKEAINYKVPEdmpiEKAILI 166 Staphylococcus ...
YP_106361    104 GAer-rgVKVGDRVISEQIVPCWSCRFCGHGQYWMCQKHDLYGFQnn-vhGAMAEYMIFTKEAIVHRVPDsiptDEAILI 181 Burkholderia ma...
YP_274915     80 GAek-rgVQIGDRVISEQIVPCWGCRFCNHGQYWMCQKHDLYGFQnn-vqGAMAQYMIFTKEGIIHKVPDsiapDEAILI 157 Pseudomonas syr...
YP_003013071  91 NVr---eFAVGDRVTADQIVPCETCRFCKRGQYWMCQPHNILGFQte-nnGGMAEYVRYPKTSVIHQVPHamklEDAVLI 166 Paenibacillus s...
YP_003323781  88 GAgekygLQVGGLAVSEQIVPCWRCRFCRRGQYWMCQQHDVYGFRqr-tfGAMAEYMLWPADAINHKVPPdvppHHAAFI 166 Thermobaculum t...
Feature 1         #   #                   ######                  ##   #                        #
YP_002508080 164 -EPYACSKHAVDRANIsnEDVVVLSGAGTLGLGMVGAIKLRNPKsLVVLDLKDdRLELAKKFGADItmnp--kkeNVVEK 240 Halothermothrix...
YP_001896848 170 -EPLACAIHTVNRGEVqlDDVVVIAGAGPLGLMMTQIAHLKTPKkLVVIDLVEeRLALAREYGADVtinp--kqdDALAI 246 Burkholderia ph...
NP_668172    185 -EPYACAFHAVQRAKIklGDVVVLAGSGTLGLGMIGAIKKSGAAkLIVLDLSDdRLALAKKFGADLvln---psrDDVNT 260 Yersinia pestis...
NP_993646    202 -EPMACAIHTVARGDIqlDDVVVLAGAGPLGLCMVQVARLKTPKkLIVIDTIEeRLALAKAFGADVvinplkedaDKIVK 280 Yersinia pestis...
YP_001361838 190 aEPLSCALHAVERADIrfGDTVVVAGCGPIGLGMIAGAAAKFPQrIIALDALPqKLELARKCGADVtlni--tevDVVEE 267 Kineococcus rad...
YP_300614    167 -EPYACSLHAIQRANIklGDFVVLSGAGTLGLGMVGAAKKAGAEtLVVLDMKDdRLELAKAFGADIvmnp--skvDVVKE 243 Staphylococcus ...
YP_106361    182 -EPLSCSLHAADRANVgfDDVVVVAGAGTLGLGIIGAARLRHPKqLIVLDMKPeRAALARRMGADDvwnp--aeeNVIEK 258 Burkholderia ma...
YP_274915    158 -EPLACSLHAAERANVdfDDIVVVAGAGTLGLGIIGAVRMRNPKkLIVLDMKPeRAALALRMGADEvwnp--aevDVLAK 234 Pseudomonas syr...
YP_003013071 167 -EPYACSKHAVDRAQIgcEDIVVISGAGPLGLGMITYARMKNPKkLIVLDLIDsRLDKATEFGADIaln---ptkQDVNQ 242 Paenibacillus s...
YP_003323781 167 -EPLACAIHAVDRGEIkfEDVVVIAGAGPLGLGMVAAARMRNPRlLVVVDLEDrRLEVARRCGADLtlnp--skvDVVEE 243 Thermobaculum t...
Feature 1                        ## #                   ##                       ###             
YP_002508080 241 Il-eMTDGYGCDIYIEATGHPssVQQGLEAIRKLGTFVEFSVFgDLVTTDWSIISDRKELDVLGSHLGPYCYEPVIEWIA 319 Halothermothrix...
YP_001896848 247 Ih-sLTDGYGCDVYIETTGAPigVNQGMDLIRKLGRFVEFSVFgADTTLDWSVIGDRKELDVRGAHLGPYCYPIAIDLLA 325 Burkholderia ph...
NP_668172    261 LikeMTGGYGCDIYIDATGAQkaVEQGLTLLRKLGTFVEFSVFkDPVTVDWSIISDRKELDVLGSHLGPYCFPLVIEGIS 340 Yersinia pestis...
NP_993646    281 S---LTGGYGCDVYIEATGAPigVTQGLQMIRKLGRFVEFSVFgQETTVDWSIIGDRKELDIRGAHLAPYSYEIATDLFE 357 Yersinia pestis...
YP_001361838 268 Vk-kLTGGYGADVYIEGTGHPsaVAQGLNLLRKLGTFVEYSVFkDPVTVDWSIISDDKELNVLGAHLGQNTWPAALRLIA 346 Kineococcus rad...
YP_300614    244 Ik-dMTEGYGCDTYIEATGHPksVEQGLSAIRKLGRFVEFSVFgDPVTVDWSIISDRKELDLMGSHLGPYCYPLVIDGIQ 322 Staphylococcus ...
YP_106361    259 Ir-aITGGYGCDIYIEATGHHraVGQGLAMLRKLGRFVEFSVFnDEASVDWSIIGDRKELDVLGSHLGPYMYPRAIEFIA 337 Burkholderia ma...
YP_274915    235 Ir-eITGGYGCDIYIEATGHHkaVNQGLAMLRKLGRFVEFSVFnDEATVDWSIIGDRKELDVLGSHLGPYMSPRAIDFIG 313 Pseudomonas syr...
YP_003013071 243 IildMTEGYGCDIYIEATGHPssVTQGLNMIRKLGRFVEFSVFgAPTTVDWSIIGDRKELDILGSHLSPYCFPFVIENIA 322 Paenibacillus s...
YP_003323781 244 Vl-gLTEGYGCDVYIEATGHPaaVEQGLRMIRKLGTFVEFSVMrEPVTVDWTIIGDTKELNIHGAHLGPYCYPIAIDMIR 322 Thermobaculum t...
Feature 1                                             
YP_002508080 320 NGkLPTEGVVTHKFPLeKWKEAFEKaEKGQDSIKVVL 356 Halothermothrix orenii H 168
YP_001896848 326 RGlVTSKGIVTHGFSLeEWDEAIKI-ANSLDSIKVLL 361 Burkholderia phytofirmans PsJN
NP_668172    341 NGdLPTEGVVTHILPLeKYAEGIELvKRGVDSLKVVL 377 Yersinia pestis KIM
NP_993646    358 RGlVTSKGIVTHSYSLdDWEKAFEL-ADSTNSIKVIL 393 Yersinia pestis biovar Microtus str. 91001
YP_001361838 347 SGrLPLDEICSHQLPLaRFQEGLDLvADSANSVKVSL 383 Kineococcus radiotolerans SRS30216
YP_300614    323 KDdFPTDGVVTHQLPLkDFEEGFELmKKGDQSLKVVL 359 Staphylococcus saprophyticus subsp. saprophyticus ATCC 15305
YP_106361    338 SRrIDVRGIVTHTFPLsRFADAFAVmERGKQSLKVVL 374 Burkholderia mallei ATCC 23344
YP_274915    314 NRkIDMRDVVTHKFPLaEFKEAFAVmERGDKSLKVVL 350 Pseudomonas syringae pv. phaseolicola 1448A
YP_003013071 323 SGnLKTSGVVTRQFPIeQWEEAFEYaSGKHGDFKVVI 359 Paenibacillus sp. JDR-2
YP_003323781 323 KGrLPLDEIVTHRLPLeDFHRGIEImMSREHSIKVVL 359 Thermobaculum terrenum ATCC BAA-798

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