1YB5


Conserved Protein Domain Family
zeta_crystallin

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cd08253: zeta_crystallin 
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Zeta-crystallin with NADP-dependent quinone reductase activity (QOR)
Zeta-crystallin is a eye lens protein with NADP-dependent quinone reductase activity (QOR). It has been cited as a structural component in mammalian eyes, but also has homology to quinone reductases in unrelated species. QOR catalyzes the conversion of a quinone and NAD(P)H to a hydroquinone and NAD(P+. Quinones are cyclic diones derived from aromatic compounds. Membrane bound QOR acts in the respiratory chains of bacteria and mitochondria, while soluble QOR acts to protect from toxic quinones (e.g. DT-diaphorase) or as a soluble eye-lens protein in some vertebrates (e.g. zeta-crystalin). QOR reduces quinones through a semi-quinone intermediate via a NAD(P)H-dependent single electron transfer. QOR is a member of the medium chain dehydrogenase/reductase family, but lacks the zinc-binding sites of the prototypical alcohol dehydrogenases of this group. Alcohol dehydrogenase in the liver converts ethanol and NAD+ to acetaldehyde and NADH, while in yeast and some other microorganisms ADH catalyzes the conversion acetaldehyde to ethanol in alcoholic fermentation. ADH is a member of the medium chain alcohol dehydrogenase family (MDR), which has a NAD(P)(H)-binding domain in a Rossmann fold of a beta-alpha form. The NAD(H)-binding region is comprised of 2 structurally similar halves, each of which contacts a mononucleotide. The N-terminal catalytic domain has a distant homology to GroES. These proteins typically form dimers (typically higher plants, mammals) or tetramers (yeast, bacteria), and have 2 tightly bound zinc atoms per subunit, a catalytic zinc at the active site, and a structural zinc in a lobe of the catalytic domain. NAD(H)-binding occurs in the cleft between the catalytic and coenzyme-binding domains at the active site, and coenzyme binding induces a conformational closing of this cleft. Coenzyme binding typically precedes and contributes to substrate binding. In human ADH catalysis, the zinc ion helps coordinate the alcohol, followed by deprotonation of a histidine, the ribose of NAD, a serine, then the alcohol, which allows the transfer of a hydride to NAD+, creating NADH and a zinc-bound aldehyde or ketone. In yeast and some bacteria, the active site zinc binds an aldehyde, polarizing it, and leading to the reverse reaction.
Statistics
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PSSM-Id: 176215
View PSSM: cd08253
Aligned: 33 rows
Threshold Bit Score: 239.023
Threshold Setting Gi: 21219369
Created: 28-Jan-2009
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
NAD(P) binding
Conserved site includes 27 residues -Click on image for an interactive view with Cn3D
Feature 1:NAD(P) binding site [chemical binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                      #   #                             
1YB5_A        30 MRAVRVFEF----GGPEVl-KLRSDIAVPIPkdhQVLIKVHACGVNPVETYIRSGtysr--kplLPYTPGSDVAGVIEAV 102 human
YP_002488157   1 MKAIVYEET----GASSV--LQLQDRATRDPgpgEVRVRLAVSGVNPTDWKSRAGsgqgstklkTPKVPNQDGAGIVDAV 74  Arthrobacter ch...
YP_001363341   1 MKSIIHTET----GPASV--LRLEERPLPVPgagEVRVRVVVSGVNPTDWKSRRGrgngqalafPEITPNQDGAGVVDAV 74  Kineococcus rad...
YP_707639      1 MKAVIYTRT----GGPEV--LQLVEKAMPQIpagHVRVRLVVSGVNPTDWRSRSGqssg--mkfDEQVPNQDGAGVIDAL 72  Rhodococcus sp....
YP_295813      1 MKAIWYEKQg---RAEEV--LAFGELPTPQPapgEVRVRLHASAVNPADANRRAGrmhg-gmefPRIIPNSDGAGVIDAL 74  Ralstonia eutro...
NP_898787      3 MRAATYHKSg---EASDVlsIIQCEKPEPGPg--QVLVRVSFSAVNPSDVKTRSGktpr--pidDFQIPHMDGCGRIVAV 75  Rhodococcus ery...
NP_241801      1 MKAVVYEQY----GDPSV--LKVMDVPKPTIgptDVLINVKASGTNPVDTYFRKGirq---veqFPHIPHFDVAGEIVEI 71  Bacillus halodu...
YP_923614      1 MRAVVYQQT----GPSSVleVVERDLPVPGPg--EVRVRVVRAGVNPTDWKFRAGmmt----eyDEVVPGQDGAGVVDAV 70  Nocardioides sp...
NP_625148      9 MRAAYIDAV----GPADA--IRYGELAVPPVgptDVLVRVAAVAADPVDTFVRSGayat--plpLPFVVGRDLVGEVAVA 80  Streptomyces co...
YP_003199532   1 MPATRLAAYvdqrGPADS--IRLGRLPVPELgpdDVLVAVTAVSVNPVDTYVRSGrydt--pipLPFIVGRDLVGTVAEI 76  Nakamurella mul...
Feature 1                                                              #   #                     
1YB5_A       103 GDnasafkKGDRVFTSst----iSGGYAEYALAAdhtVYKLPEKLDFKQGAAIGIPYFTAYRALIh--------sACVKA 170 human
YP_002488157  75 GPgvtgfsAGDRVWLWdvawgsdEGTAQEYAVVPsvkVVALPDDESFDTGASLGIPALTAHRALTssehgpdrlsPGSLT 154 Arthrobacter ch...
YP_001363341  75 GPgvsdlaAGDRVWTCmaaqgrpTGTAQEFTVLPaerVVRLPDGVSFDVGASLGVPAVTAHRALTvaedgprrlaPGALA 154 Kineococcus rad...
YP_707639     73 ADdvngffVGQRVWLWdvaygraSGTAQEYVVIParhAVALPETASFDVGASLGIPALTAYRTLTsaesgpsrlhPGALT 152 Rhodococcus sp....
YP_295813     75 GHgvdqkmLGTRIWLHfgqrgrpFGTAAEYICVPhelTSPLPEHLDFAQGACLGIPAMTAYIALFq---------RGAIK 145 Ralstonia eutro...
NP_898787     76 GPgvhegrIGQRVWLWlaalgsrWGTAAEYTLVPanqAVALPDSASDELGACLGIPAMTAHECLLh---------AGPVT 146 Rhodococcus ery...
NP_241801     72 GSdienwqVGDRVWATn-----iKGASAEYALIPehlLFPLPKSVSYEEGAALAMTFMTAHLSLFd--------rGRLQK 138 Bacillus halodu...
YP_923614     71 GEgvdglaVGDRVWLVlaqagraYGTAAELTVQPaarVVPLPADASFDLGASLGVPAVTAHRALTaaeggpsrlwPGALE 150 Nocardioides sp...
NP_625148     81 GPgaaglaVGQRVWCNslghagrQGSFAQYATVAaerLYPAPAGIDAEHLVAAAHPAASAWLALFr--------hGRLRA 152 Streptomyces co...
YP_003199532  77 GSavrdlaVGDPVWANslghagrQGAATELAVVArdrLYRLPAGVDPELAVAVAHPAVTAYLGWFl--------rARIRP 148 Nakamurella mul...
Feature 1              #######                  ##                   ##                      ##  
1YB5_A       171 GESVLVHGASGGVGLAACQIARAYGLKILGTAGt-EEGQKIVLQnGAHEVFNHREvnYIDKIKKyvgEKGIDIIIEMLAN 249 human
YP_002488157 155 GQTVLVTGGAGAVSHAAIQLARWAGATVITTVSd-DRKDGLARLaGAHRVLNYRTedVVAAVNAa-vPEGVDTIVDVNAP 232 Arthrobacter ch...
YP_001363341 155 GRTVLVSGGAGAVGHAAIQLARWAGARVVTTVSg-PEKARLATAaGADHVVNYREgdPAAAIREv-aPEGVDLVVEVAIA 232 Kineococcus rad...
YP_707639    153 ASSVLVHGGAGAVGHAAIQLAVWAGATVITTVSn-AEKAQLARNaGAHHIINYRTedVASRIAAl-aPNGVQRIVEVDLA 230 Rhodococcus sp....
YP_295813    146 GKYVLVTGGAGAVGHYAVQLAKWGGAHVIATASsaRKAAHAAAG-GADAVVDYRApdAAQQILDathGQGVDHIVDVNAI 224 Ralstonia eutro...
NP_898787    147 HKKVLIAGGAGAVGHFAIELARWAGATVIATVSt-PDKAKLARQaGADFVVNYRDddVADQITAf--TNGVDIFVEVAIV 223 Rhodococcus ery...
NP_241801    139 GETVLIYGGSGAVGHAAIQLAKRAGATVITTAGd-REKGEIAKQaGADQVIFYKEesVVDAVGKatnGQGVPLILDMSLS 217 Bacillus halodu...
YP_923614    151 GRVVLVAGGAGAVGNAAIQLARWAGATVVTTVSs-AEKAALARAaGADHVVNYREpgAAQAILEt-aPDGVDLAVEVAPA 228 Nocardioides sp...
NP_625148    153 GETVYVGGGAGNVGGAAVALASRAGARVVATAR--AEDAAAVRQlGAAEVLDHHApdLGARVRGa-aPDGYDVHLDTSGH 229 Streptomyces co...
YP_003199532 149 GWTVFVGGGAGNVGIAAIEMATRAGVRVIASAR--DDHADRCRAaGAQAVVDYRAadLAERIRSa-aPNGVDVYWDTSGT 225 Nakamurella mul...
Feature 1         #                ##     #                   ###                                
1YB5_A       250 vNLSKDLSLLShGGRVIVVGS----RGTIEINPrdTMAKESSIIGVTLFSStkEEFQQYAAALQAGMEI-GWLKPVIGSQ 324 human
YP_002488157 233 aNLESDVEILKpGGTIAIYAAnpgeSVTVPVRE--SMGKNIRYQFILTYTVtdGQKQHAVESVSEALAA-GALRVGEEHG 309 Arthrobacter ch...
YP_001363341 233 pNLEFDQAVVRpRASIASYANnggdDVRVDIRA--HMVLNTRLQFILMYTMgdEALSAAFEDITAALRD-GALPVGEEHG 309 Kineococcus rad...
YP_707639    231 aNLEVDVSVIApNGTISVYTFvngeTLRTPSLG--LLVKNVHISFTYTYTTrpDQKELAVASVNAAVAD-GAMKVGTEHG 307 Rhodococcus sp....
YP_295813    225 dNSRLCFEAAAnHAQWVSYASgaglEQAVPLVT--LIRKNLCLQGLYLPGLpyEMRWIAQEGLSRWMHDvPNAIHAVDSV 302 Ralstonia eutro...
NP_898787    224 dNWNIDLAVAApGASIATYATdg-rELSIPIRE--CMAAGLGLRFFLLYTHsrSALSLAAQSITDALAE-QALTPLPHLT 299 Rhodococcus ery...
NP_241801    218 eNMAQDFDILTtGGRIVAIGSpvdnTPPLLWRQ--LNMKHASLIGVLVFTApkNVLRQAGKEIAEGFEQ-GELSAYIGKV 294 Bacillus halodu...
YP_923614    229 qNIELDLDVVRvHGTVSIYANnggdTLTMPLRA--AFGKNLRFQFLILYTLreDLLRAAVEDISVALAA-GALRVGEEAG 305 Nocardioides sp...
NP_625148    230 gVLAEAVDSLArGGRLVAMVGlgagPAALSVGG--LYTKDASIVGFAISNAtaADLAEAARGVVGVLTD-TPWRPRIADR 306 Streptomyces co...
YP_003199532 226 nDTAVAADVLRpGGRMLVTAAr--sPVTVNLAP--LYTHDVTIDGFVISRAsaAEFAQAAELINAMLAA-GELTTRITDR 300 Nakamurella mul...
Feature 1                         #    ##        
1YB5_A       325 YPLe-----kVAEAHENIIhgsGATGKMILLL 351 human
YP_002488157 310 LPLtrfplaeTASAHDAVEq--GTIGKVLIDV 339 Arthrobacter chlorophenolicus A6
YP_001363341 310 LPLlrfplerTAAAHEAVEg--DAVGKVLVDV 339 Kineococcus radiotolerans SRS30216
YP_707639    308 LPLerydldrTAEAHVAVEr--GTIGKVLIDI 337 Rhodococcus sp. RHA1
YP_295813    303 FDLq-----dTEKAHQAVEa-gAKLGTVVVQC 328 Ralstonia eutropha JMP134
NP_898787    300 FPLd-----eIALAHEAVEn--GESRKVLLDL 324 Rhodococcus erythropolis
NP_241801    295 FPId-----eAAHAHRALEa-kTYQGRIILSH 320 Bacillus halodurans C-125
YP_923614    306 LPLhhyplaqAAEAHDAVEg--GVVGKVLLDV 335 Nocardioides sp. JS614
NP_625148    307 LPLs-----mTAEAHRRLEa-gEVRGRLVLRP 332 Streptomyces coelicolor A3(2)
YP_003199532 301 LPLa-----qAAQAHARMEs-gPVDGRLLLLV 326 Nakamurella multipartita DSM 44233

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