3GQV


Conserved Protein Domain Family
enoyl_reductase_like

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cd08249: enoyl_reductase_like 
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enoyl_reductase_like
Member identified as possible enoyl reductase of the MDR family. 2-enoyl thioester reductase (ETR) catalyzes the NADPH-dependent dependent conversion of trans-2-enoyl acyl carrier protein/coenzyme A (ACP/CoA) to acyl-(ACP/CoA) in fatty acid synthesis. 2-enoyl thioester reductase activity has been linked in Candida tropicalis as essential in maintaining mitiochondrial respiratory function. This ETR family is a part of the medium chain dehydrogenase/reductase family, but lack the zinc coordination sites characteristic of the alcohol dehydrogenases in this family. NAD(P)(H)-dependent oxidoreductases are the major enzymes in the interconversion of alcohols and aldehydes, or ketones. Alcohol dehydrogenase in the liver converts ethanol and NAD+ to acetaldehyde and NADH, while in yeast and some other microorganisms ADH catalyzes the conversion acetaldehyde to ethanol in alcoholic fermentation. ADH is a member of the medium chain alcohol dehydrogenase family (MDR), which has a NAD(P)(H)-binding domain in a Rossmann fold of a beta-alpha form. The NAD(H)-binding region is comprised of 2 structurally similar halves, each of which contacts a mononucleotide. The N-terminal catalytic domain has a distant homology to GroES. These proteins typically form dimers (typically higher plants, mammals) or tetramers (yeast, bacteria), and have 2 tightly bound zinc atoms per subunit, a catalytic zinc at the active site, and a structural zinc in a lobe of the catalytic domain. NAD(H)-binding occurs in the cleft between the catalytic and coenzyme-binding domains at the active site, and coenzyme binding induces a conformational closing of this cleft. Coenzyme binding typically precedes and contributes to substrate binding. Candida tropicalis enoyl thioester reductase (Etr1p) catalyzes the NADPH-dependent reduction of trans-2-enoyl thioesters in mitochondrial fatty acid synthesis. Etr1p forms homodimers with each subunit containing a nucleotide-binding Rossmann fold domain and a catalytic domain.
Statistics
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PSSM-Id: 176211
View PSSM: cd08249
Aligned: 229 rows
Threshold Bit Score: 202.814
Threshold Setting Gi: 170940385
Created: 28-Apr-2009
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
NAD(P) binding
Conserved site includes 20 residues -Click on image for an interactive view with Cn3D
Feature 1:NAD(P) binding site [chemical binding site]
Evidence:
  • Structure:3GQV; Aspergillus terreus Lovastatin polyketide enoyl reductase (Lovc) Mutant K54 binds NADP, contacts at 4A
    View structure with Cn3D
  • Comment:MDR family binds NAD(P) as a cofactor

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                         ##                              
3GQV_A         11 QQTALTVndh---deVTVWNAAPCPmlp------rdQVYVRVEAVAINPSDTSMRgqfatpWAFLGTDYAGTVVAVGSDV 81   Aspergillus t...
XP_001211606    5 NRALYVNdd----geFSVRDDIPPHniq------pnELLIETHYSGVNPADVKHSahlgiqPTVIGYDFSGRVLSAPPSS 74   Aspergillus t...
EED55107        7 NTGLYADeh----aiFRVRRDLQFPvpe------egELLIETQFSGANPADVKHAtflgiyPAVLGYDFCGKVLQAPPQS 76   Aspergillus f...
EEU37904        5 TKALFVTqnv--qfeVIQKQELPKPae--------gELLIKVLFSGANPADIKHStelgivNTTLGYDFCGEVIETTNPD 74   Nectria haema...
CAP65612        8 PTALFLSsd-----gEITPIAAPFPvaaslplapqtHVPIKVTHSAIQPADKKHFnlg-fhSSITGFDYAGIDLTTNTPV 81   Podospora ans...
XP_001791715    6 PKAIYVDengdfsirDDILHKVTAPn----------DVHIEVIYSGVNPADTKHAhlg-irSTVIGYDFCGRVLSVPSGS 74   Phaeosphaeria...
XP_383691     630 NKAIYMLseg--nheTRIIQEPYIPks--------aQSLVDVQYSGINPADTRHMymn-mtNYVAGYEFTGTVKQIGPES 698  Gibberella ze...
XP_002381787    3 NTALYADen----geLCGRRDLPIPqpi------egEILIQVLFTGVNRSDITSVkllgsrNRVLGNDFCGRVLDVSGLG 72   Aspergillus f...
XP_384839      10 STALYANae-----gKPVVTRLPMPalr------ddEILIKVMYAGVNPSDFRVLkffdcrRRVMGHEFCGKVLESPGLA 78   Gibberella ze...
EEU35593        6 NKAIYLDsqg--khtIRIIEEQYVPtg--------aQSLVEVKYSAINPADTRHYfmg-isEVVSGYEFVGTIRETGPDS 74   Nectria haema...
Feature 1                                                                   #                     
3GQV_A         82 Th-IQVGDRVYGAqnemcprtpdqGAFSQYTVTrgrvwAKIPKGlSFEQAAALPAGISTAGLAMKLlglplpspsa---d 157  Aspergillus t...
XP_001211606   75 K--FSPGDIVAGYtpsglgrptryGAHQDVLAVpedmaFKVPPHlPEADAAALTVVAMTAADVVHNlfkfplpt------ 146  Aspergillus t...
EED55107       77 S--FSPGDTVAGYtptglnrpagyGTHQQYLVCpedmaFHVPANlPPHHAACLSVVTMTAADTLYSifkfplpgt----- 149  Aspergillus f...
EEU37904       75 Ss-YKAGDIVAGYtpsglgrpskyGSHQAYLTCpetmlFKVPDNlPQSDAACLAVVAMTAADAIYNhlgfplpge----- 148  Nectria haema...
CAP65612       82 LglTWPGPHRPLHa----------GAHQPYILArsdliWTRPEGlSAEAAAGLPTALLTAADAFVNifgvgfasagieql 151  Podospora ans...
XP_001791715   75 K--LTKGDIVAGYtpsgmgrpsryGTHQAHCYCpedmiFSVPSHlPQSHAAAITVVAMTAADTVFNifklplpt------ 146  Phaeosphaeria...
XP_383691     699 P--FKIGQDVFGIslmydrrpnylGAHQSFLLAeplmtFLRPDHlDPITAVTLLAGGGTAMDGLFNvlgygfspad---- 772  Gibberella ze...
XP_002381787   73 DtaFEPGDIVAGYtianhdrplryGSHQSYISIppkniFKVPENvPPADAAGLMTVVQTANDALLNlhlplpsm------ 146  Aspergillus f...
XP_384839      79 KspFKAGDIVAGYvngsndrperfGTQQEYISIppawaFEVPDNvPHPNVAGLPVVVQTANDAIFNrfklplpq------ 152  Gibberella ze...
EEU35593       75 P--FKPGQEVFGFsipgdrrplhlGAHQDFLLAdnlltYERPEDmDATSAVTMLAGALTAIDGLFNtleyglpaag---- 148  Nectria haema...
Feature 1                         ####                   ## ##              #                     
3GQV_A        158 qppthskpvYVLVYGGSTATATVTMQMLRLSGy-iPIATCSpHNFDLAKSRGaEEVFDYRApnlaqti----rtytknnL 232  Aspergillus t...
XP_001211606  147 --spgsfsaPLLIWGASSSVGICAVQLARASGctnILVTASpSRHALLRSFGaSHAFDYSSpsvvadiqsvvetlgqgpI 224  Aspergillus t...
EED55107      150 -kdtektgrPVLIWGASSSVGLCAVQLARASGiypIFVTASpQRHPLLLELGaTQCFDYRSpdvvsqiksaleqgqwssI 228  Aspergillus f...
EEU37904      149 -tekfekngPFLLWGATGAVGYCALQFAVASGispVIVAAKpEQFEHLESLGaTKCFDYRAedvyesigtalkelgyddF 227  Nectria haema...
CAP65612      152 psgthsqgkGALIWGGASAVGWAAIQVAREIGvefIFVVAStKNHDLLRGIGaTHVFDYTHpeettkeikrtlqsldvtL 231  Podospora ans...
XP_001791715  147 --spavhncPIVIWGASSSVGICALQFLKASGcqnILVTASlGRHELLKSLGaTHTFDYNSltveadiaaavqalgqgsV 224  Phaeosphaeria...
XP_383691     773 ipgdnpkdePILIWGGAGSVGQAAIQLAKAAGfypIITTAStQNHETMKGIGaSHTFDYKSptvtkdirs-llessgwsL 851  Gibberella ze...
XP_002381787  147 --tqgptdgVFVIAGGATTVGISTIQLARSIGiksIIVTASpGRHELLESLGaTRCFDYRKdsvvqdika-aveevglgP 223  Aspergillus f...
XP_384839     153 -dakgpiegTMVIWAGATCVGMSAIQLARASGvtsIIVTASpKRHEFLKKLGaTHCFDYSDestparika-lvesagdvP 230  Gibberella ze...
EEU35593      149 legddptdvPILIWGGSSVVGQGAIKLAKAAGfnpILTTASpHNHDALKQLGaTHCFDYRSptivqdiha-tmkglnkkL 227  Nectria haema...
Feature 1              ##                                                               ##        
3GQV_A        233 RYALDCItn-------------veSTTFCFAAIGra----------------------------gGHYVSLNPfpeha-- 269  Aspergillus t...
XP_001211606  225 THAFDAVgslg----------dpsSADMVAQCVDs-----------------------------sAVLVSVVLrd----- 260  Aspergillus t...
EED55107      229 DYAFDTVgsyg----------gtgSAQLMAECVSe-----------------------------eACLVSVVVqr----- 264  Aspergillus f...
EEU37904      228 TYAFDAAga-------------psSGDQVLRCISe-----------------------------kTAAASTLFre----- 260  Nectria haema...
CAP65612      232 TAVFDAVgagledagstyensscyWGKQCLSGDQe-----------------------------rDALACARP------- 275  Podospora ans...
XP_001791715  225 SHALDAAgtft----------qpsSADMLARAMNsp----------------------------gAILASVTKfhggfq- 265  Phaeosphaeria...
XP_383691     852 KTVFDAVs----------------TGLGVFEGLTqeqekeiqdkydqsssgmarqccdpkvpsseLRLTSVLP------- 908  Gibberella ze...
XP_002381787  224 VFGLDAAat-------------pdAAKILLDALAdr---------------------------ddTHLASLSPwa----- 258  Aspergillus f...
XP_384839     231 VWGFDALgtmt----------tpsPQTLLASSLPrs---------------------------drVKLVTVLLmpeegfe 273  Gibberella ze...
EEU35593      228 KTVFDSVa----------------TGLGVFEGLTkeee-----------------------eaiqAKYEESSPgiarrcc 268  Nectria haema...
Feature 1                                  # #                                                    
3GQV_A        270 ---------------atrkmVTTDWTLGPtifgegstwpa----------pygrpgseeerQFGEDLWRIAGQLVE---D 321  Aspergillus t...
XP_001211606  261 -------------------dRRFRMPLATtnnpfsiqppg----------vpqpisiparpADHWNAWKALNWAVDn-yG 310  Aspergillus t...
EED55107      265 -------------------dPKFKMPIATpnrdctirvhg----------aprpitiparpSDYVRAWQALQWAVTnygA 315  Aspergillus f...
EEU37904      261 -------------------sKQFKFPIATpnldftiqlag----------vphpvtvparpADYEKAWKALAWAVEn-yG 310  Nectria haema...
CAP65612      276 --------------------VPREWDSDWefaifsrkw---------------galpkqspEWTWSRQDKVMRWFLenhA 320  Podospora ans...
XP_001791715  266 ---------------mplatTKDEWRFLPpgapepi-------------------sipgnpERHWNAWTALEWAIKq-yN 310  Phaeosphaeria...
XP_383691     909 --------------------VKKDPTYAFclnfrpvevldignegaewseefkqaevakmkTWQARIEKGIEWLVQn-hE 967  Gibberella ze...
XP_002381787  259 -------------------gDRFEFVLGGrdydvifhppg----------apepfvslarpADAAKMWDSLMWVLQh-yG 308  Aspergillus f...
XP_384839     274 -------------pcigarhYQTSFDLPNgdkvd----------------------ippnmEAADRMWKALGWVMDh-yG 317  Gibberella ze...
EEU35593      269 dpdipedelrlsaslvvakdLAWKFTLMFrtv--------------------------dtmDHGVARNDYTIRWLIknhA 322  Nectria haema...
Feature 1                                                      ##       
3GQV_A        322 GRLVHHPLRVvqggfdhIKQGMELVRKGe---------------lSGEKLVVRL 360  Aspergillus terreus
XP_001211606  311 KAFKLPAVETle---vkAEEALEEIYKVadg------------krGFGKIVFQH 349  Aspergillus terreus NIH2624
EED55107      316 GFQFPSVDVVsg----tAENALEQVQALgdg------------trGFGKLALEH 353  Aspergillus flavus NRRL3357
EEU37904      311 RAFRSVPVRVld---gtAEDSLEEIQRVvkv------------ggGFAKIAIQQ 349  Nectria haematococca mpVI 77-13-4
CAP65612      321 AAWKPMPTRVvrs-kedAVVAINSSAEGr---------------iSCEKVVIRH 358  Podospora anserina
XP_001791715  311 TRFMLPSVEIld---stPEAALDEIIKVgnsgrgiwetscsrqpmKQMTVNYRL 361  Phaeosphaeria nodorum SN15
XP_383691     968 QYWEPPRTRVvk----gVEEGLQGIRDVwqg------------kvSREKLIIDH 1005 Gibberella zeae PH-1
XP_002381787  309 KEFKLPVIEVfe---gpAEEALEELEKVad-------------qgKFGKLVLKH 346  Aspergillus flavus NRRL3357
XP_384839     318 KEFQEIPVEVfk---gtAEDALKEVEKVestg----------rfgNHVPLTVPL 358  Gibberella zeae PH-1
EEU35593      323 KHWQAPRTRVve----tAEEGIQAIKDVysg------------ktSREKVIIKH 360  Nectria haematococca mpVI 77-13-4

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