Conserved Protein Domain Family
AST1_like

?
cd08247: AST1_like 
AST1 is a cytoplasmic protein associated with the periplasmic membrane in yeast
This group contains members identified in targeting of yeast membrane proteins ATPase. AST1 is a cytoplasmic protein associated with the periplasmic membrane in yeast, identified as a multicopy suppressor of pma1 mutants which cause temperature sensitive growth arrest due to the inability of ATPase to target to the cell surface. This family is homologous to the medium chain family of dehydrogenases and reductases. Medium chain dehydrogenases/reductase (MDR)/zinc-dependent alcohol dehydrogenase-like family, which contains the zinc-dependent alcohol dehydrogenase (ADH-Zn) and related proteins, is a diverse group of proteins related to the first identified member, class I mammalian ADH. MDRs display a broad range of activities and are distinguished from the smaller short chain dehydrogenases (~ 250 amino acids vs. the ~ 350 amino acids of the MDR). The MDR proteins have 2 domains: a C-terminal NAD(P) binding-Rossmann fold domain of an beta-alpha form and an N-terminal catalytic domain with distant homology to GroES.
Statistics
?
PSSM-Id: 176209
View PSSM: cd08247
Aligned: 20 rows
Threshold Bit Score: 377.379
Threshold Setting Gi: 254568406
Created: 6-Aug-2009
Updated: 2-Oct-2020
Structure
?
Aligned Rows:
 
putative NAD(P)
Feature 1:putative NAD(P) binding site [chemical binding site]
Evidence:
  • Comment:MDR family binds NAD(P) as a cofactor
  • Comment:defined by comparison to MDR alcohol dehydrogenase like family

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
Feature 1                                                          ###  #                        
P35183        50 IKSLIFHTKTGPmdfsyeKKIKTPIP-----------KNKIVVRVSNVGLNPVDMKIrn--gYTSSIYg------EIGLG 110 baker's yeast
XP_454874     50 VKSLIFYSKNHPpqfsyeTKIKTPIS-----------KDVISVQVEYVGLNPVDLKIfn--sYTRHMNy------EVGIG 110 Kluyveromyces l...
XP_001647419  37 TKALVFQTKTGPvdftyeNKIKTPIG-----------SSKLVVDVRYVGLNPVDLMIkn--gYNQQGFy-----gEIGLG 98  Vanderwaltozyma...
NP_987039     51 VKALKFYSRQKPpqfdydTPILLPIR-----------PSKLVVQVSYVGLNPVDIKImn--gYAKHLSg------KIGLG 111 Ashbya gossypii...
XP_002495102  59 LKSLIFYSKRGPlefsydGKIKLPVP-----------KNKLVVEVHYAGLNPVDMKIkn--gYQTGIYg------AAGLG 119 Zygosaccharomyc...
CAR22077      51 VKSLAFFSKNNPprfsyeTRIKTPVP-----------KDKLVVQVRNAALNPVDLKImn--sYTHNMNr------EVGLG 111 Lachancea therm...
EEQ38559       5 CKAVTYTTNASPlt---lKTVSLPVEqkdgq--fvvgENDILVKVHYAALNPVDSILkn-saYGWLSKs------EKGFG 72  Clavispora lusi...
EAL02279      10 FKAYTYKNSSTPvql-tdKSIKLVKDpsnpd-syvapPGKILLKINYTSLNPVDVKLhhlatSLVSLLvn----nNNGFG 83  Candida albican...
EDK39587      10 YEAVEYANNCSEpq---wVPKKLTLTkqknseeygagDHQILVRVHAASLNPVDSMLyn--tYNRVVGyl---kgRSGMG 81  Pichia guillier...
XP_457183     10 FNAITYSSNDKPma---iSKQSISLAkqadg-synvpPNKILVKVHSAALNPIDLVL-----YNSAFKvlsyynsQQGIG 80  Debaryomyces ha...
Feature 1                                                                                #   #   
P35183       111 REYSGVITEVGENLny--aWHVGDEVYGIYYHPhlavGCLQSSILVDPKvd---piLLRPESVSAEEAAGsLFCLATGYN 185 baker's yeast
XP_454874    111 REYYGKISEVGSNLad--tWSVGDEVCGTFWHPnlgkGTCESTILVDPKvd---vvLPKPVNISTQQAAStFYCLGAAFN 185 Kluyveromyces l...
XP_001647419  99 REYFGVISDIGSKLesdnrWKIGDEVMGIYYHPhigyGSLQTSILVDPKqd---plVLKPENVTMPEAGGsLFCLASAFE 175 Vanderwaltozyma...
NP_987039    112 REYSGVISEVSDDLrd--lWRVGEEVMGTFWHPnlgkGTCETSILVDPTid---viIRKPTALDMKQAGGtLYCLGASYT 186 Ashbya gossypii...
XP_002495102 120 REFSGVITHVGENIqs--eWNEGDEVYGILFHPhqavGTLQTSILVDPRtd---piMLKPLSLSMKEASGaLYTLGTAYN 194 Zygosaccharomyc...
CAR22077     112 REYSGVITEVGGDLhs--eWKEGDEVYGVFFHPnlgyGTVQSSILVSPAqd---viLRKPSNISFQAASGtLFCLGTAFN 186 Lachancea therm...
EEQ38559      73 KDFSGDVAAIGAKAaaqtsLSVGDRVAGLNQKIfk-nGTLSEYTLVNAFdmsgkatTKIPDEMPYDKAAAfPLVLGTAHQ 151 Clavispora lusi...
EAL02279      84 RDFSGEVLSIGDNVkt--nVKVGDLVQGIYHKVys-qGTASQYLLVDPNev---dmIAKPDNISLAEASSwPTVFGTALL 157 Candida albican...
EDK39587      82 IDYSGTIVSIGVSAakktdLTVGDEVCGMYMHPff-sGTVAEYLLIDPKkdp--aiTRKPRSIDLTMASAwPLVYGTAHQ 158 Pichia guillier...
XP_457183     81 RDYSGTIVAIGEVAakktdFSIGDKVCGMYTHIlg-nGTVSEYVLLDWTsdl--svVPVPKNLSMNEAAAwPLVLGTALT 157 Debaryomyces ha...
Feature 1                            #### ##                     ##          #                 # 
P35183       186 ILnklsknkylkqDSNVLINGGTSSVGMFVIQLLKRHYKlq-kkLVIVTSANGPqvl-qeKFPDLADe-----MIFIDYl 258 baker's yeast
XP_454874    186 ILdkldqegklnqSSNILINGASTMTGIFALQLLKFHYRil--nRIVLISSTSGmn---fIKSNLPElvdellFIDYAAt 260 Kluyveromyces l...
XP_001647419 176 ILeklnslkkltpNANILINGGTSSVGLFALQLLKNYYHiv--sKVTIVTSGTGpkvikqHMPGFEKdf---iFIDYLAc 250 Vanderwaltozyma...
NP_987039    187 LLsslqergllnsKSNVLINGGTTSMGLFTIQLLKYHYGik--nNLWFVCSTKGar---yLRVHFPDllgdlvFVDYCSl 261 Ashbya gossypii...
XP_002495102 195 ILdklqrkgslqqNSNICIIGGTTSVGMAAIQLLKKHYNvstllTVVTRGNGRSvl--kkKFPSIAEei---vFINYLTc 269 Zygosaccharomyc...
CAR22077     187 ILdsleskgqlneSTNVLINGGTTSVAMFAIQLLKYHYRip--kKLVVLCSEYGat---lLKSHFPDlcdeliFVNYVMn 261 Lachancea therm...
EEQ38559     152 MFesal---kgnsYKKVLILGGGTSVGRFAVQLAKNVYGs---eEIVVTCSERSae--tlKGFGATS------YIDYSKh 217 Clavispora lusi...
EAL02279     158 MSkdl-----kykDSKVLVIGGGTSVGRYLVQLAKQGGAk----EVVVTCSPRTed--viKSLGADT------IIDYTKh 220 Candida albican...
EDK39587     159 MIeatr----vkkGDKVLVLGGATAVGRMAIQIVAQYYNp---sKIVATCSGNSap--lvKKLGADD------IIDYTSy 223 Pichia guillier...
XP_457183    158 MTqgri---evgsNAKVLVLGGSTAVGRFCIQLCRNHFKa---sEIIATCSSSSap--ivKGFGADN------TIDYTRy 223 Debaryomyces ha...
Feature 1                                                        ## #                      ##    
P35183       259 tcrgk----sskplRKMLEEkkisqydpvedketilnyneGKFDVVLDFVGGYDILSHSSSLIh---gGGAYVTTVGDYv 331 baker's yeast
XP_454874    261 ngkiykplsdlvasNELVEYnnetgnf------itqpfgqGSFNLVLDFIGGYDIIGHSSSILs---kDATYITTVGDNk 331 Kluyveromyces l...
XP_001647419 251 rgka------skplLKLLEDntytyfdeengtesienysqGKYDIVLDFIGGYDILSQSQSLIh---sGGHYVTTVGDYv 321 Vanderwaltozyma...
NP_987039    262 sgds-----vatqlKYLLSDgdaddyyaksgalqavqfdqGPFTVVLDYIGGYELIANSDSIVa---pRGIYVSTVGDAi 333 Ashbya gossypii...
XP_002495102 270 rgki------sgpiKQMIEAgqitdyddetneehtikydqGKYDIILDFVGGYDVLAHSNSLIh---kGGYYVTTVGDYv 340 Zygosaccharomyc...
CAR22077     262 arri------ykplEDMINNretisyeaesgqpisspydqGKFNLILDFVGGYQLVSHSSSLLk---kNGAYVTTVGDYr 332 Lachancea therm...
EEQ38559     218 ks----------llEPVLEHvk----------------taGPFDLILDTCGNSDLFGHMTSILrnrseGGSYISVSGDSk 271 Clavispora lusi...
EAL02279     221 kn----------ilYPVLESvk----------------stGKFDYILDSYGGHELFPEINNILte--kIGRYYSIVGDYp 272 Candida albican...
EDK39587     224 a-----------slKEPVERea----------------atAKFDVILDCVGNSDLFGTMDKIVq---rNGHYITICGEKk 273 Pichia guillier...
XP_457183    224 rs----------ldGPVIQAag-----------------sGKFDLILDCCGNDDLLSSMSKILkpkseGSHYVTIAGDNk 276 Debaryomyces ha...
Feature 1                                             ###                                        
P35183       332 -aNYKEdifdswd--npsanaRKMFGSI-IWSYNYTHYYFDPNaktasanndWIEQCGDFLk-NGTVKCVVDKVYDWKDH 406 baker's yeast
XP_454874    332 -sNYKKdtfnswg--tassniRKLFGQV-LWSFDYQMFYFDPNakla--tneWPKKCHELIesEVVKFIPVDRVYDWKEH 405 Kluyveromyces l...
XP_001647419 322 -aDYKTdvfehwd--npsasaRKMFGNI-LWSYNYTHYKFDPNgk-----teLIEKCAELLg-KRYVKCIVDNVYDWKDY 391 Vanderwaltozyma...
NP_987039    334 -aNYKTdvykywe--asgskaRGLIGKV-LWSFQYERFFFDPNakyl-aktdWATECYDLLa-ARVVRTLVDSYYPWKDI 407 Ashbya gossypii...
XP_002495102 341 -aNYKEdvynsvd--npsanmRKAFGSV-LWLYNYMHFQFDPSvsas-kksnWMGKCNELLe-RKTIKVIIDKTYDWKKH 414 Zygosaccharomyc...
CAR22077     333 -aNYAKdvfnswd--npsanvRKVFGRI-LWTFDYTHFYFDPNakya--kndWPAKCAELVe-SEVVKCVVDKVYDWKDF 405 Lachancea therm...
EEQ38559     272 -yDYSKmtvsayilnglhlvvRSLRSALgYLPYHYKLAMLDLDh-------dRTELLIRYIm-DGKVSICIDSEYPLEDF 342 Clavispora lusi...
EAL02279     273 gsSFYNlistv-----skvawRQLLGALgLLSFNYKFIMLDNFk-------eSMQEARDLIg-SGNLKIFVDSIYPFNQL 339 Candida albican...
EDK39587     274 -hKYNSisfvseaysqlfvvaRTVLCYVgYNQYNYRFLLASPGs--------WIHDGAHRL--DNGIEVAIDSTYSKADF 342 Pichia guillier...
XP_457183    277 -yRYSDvslfsiliptlfaifRMLLSMLgLNSYNFSFAAVNPEk-------qWAETGKQLIe-EEKVQVSVDSVHRVENF 347 Debaryomyces ha...
Feature 1                            
P35183       407 KEAFSYMATQRAQGKLIMNV 426 baker's yeast
XP_454874    406 QKAFSYLQTGRAHGKVVLKV 425 Kluyveromyces lactis NRRL Y-1140
XP_001647419 392 KEAFNYMKLQRAQGKLILKV 411 Vanderwaltozyma polyspora DSM 70294
NP_987039    408 RKALGYLSRGHAHGKVILEV 427 Ashbya gossypii ATCC 10895
XP_002495102 415 TEAISYMNTQRAQGKIILKV 434 Zygosaccharomyces rouxii CBS 732
CAR22077     406 TQALDYLKQGHCHGKVILDI 425 Lachancea thermotolerans
EEQ38559     343 QEAVDRQVSNKAQGKVLVKI 362 Clavispora lusitaniae ATCC 42720
EAL02279     340 DQAIEKLDSGKAAGKVVIEV 359 Candida albicans SC5314
EDK39587     343 AKAFARLRSNRASGKIIVKC 362 Pichia guilliermondii ATCC 6260
XP_457183    348 YDAIKKLQSQRANGKVVIQI 367 Debaryomyces hansenii CBS767

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap