1PS0,1LLU,1LLU,1UUF,1YQX,2HCY


Conserved Protein Domain Family
CAD

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cd08245: CAD 
Click on image for an interactive view with Cn3D
Cinnamyl alcohol dehydrogenases (CAD) and related proteins
Cinnamyl alcohol dehydrogenases (CAD), members of the medium chain dehydrogenase/reductase family, reduce cinnamaldehydes to cinnamyl alcohols in the last step of monolignal metabolism in plant cells walls. CAD binds 2 zinc ions and is NADPH- dependent. CAD family members are also found in non-plant species, e.g. in yeast where they have an aldehyde reductase activity. The medium chain dehydrogenases/reductase (MDR)/zinc-dependent alcohol dehydrogenase-like family, which contains the zinc-dependent alcohol dehydrogenase (ADH-Zn) and related proteins, is a diverse group of proteins related to the first identified member, class I mammalian ADH. MDRs display a broad range of activities and are distinguished from the smaller short chain dehydrogenases (~ 250 amino acids vs. the ~ 350 amino acids of the MDR). The MDR proteins have 2 domains: a C-terminal NAD(P) binding-Rossmann fold domain of a beta-alpha form and an N-terminal catalytic domain with distant homology to GroES. The MDR group contains a host of activities, including the founding alcohol dehydrogenase (ADH), quinone reductase, sorbitol dehydrogenase, formaldehyde dehydrogenase, butanediol DH, ketose reductase, cinnamyl reductase, and numerous others. The zinc-dependent alcohol dehydrogenases (ADHs) catalyze the NAD(P)(H)-dependent interconversion of alcohols to aldehydes, or ketones. Active site zinc has a catalytic role, while structural zinc aids in stability. ADH-like proteins typically form dimers (typically higher plants, mammals) or tetramers (yeast, bacteria), and generally have 2 tightly bound zinc atoms per subunit. The active site zinc is coordinated by a histidine, two cysteines, and a water molecule. The second zinc seems to play a structural role, affects subunit interactions, and is typically coordinated by 4 cysteines.
Statistics
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PSSM-Id: 176207
View PSSM: cd08245
Aligned: 13 rows
Threshold Bit Score: 396.692
Threshold Setting Gi: 108802912
Created: 23-Oct-2009
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 23 residues -Click on image for an interactive view with Cn3D
Feature 1:NAD binding site [chemical binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                              ###  #                                    
1PS0_A        10 IAIQshedwknpkKTKYDPKpfy--dhDIDIKIEACGVCGSDIHCaaghw-gnmkmPLVVGHEIVGKVVKLGpksnsglk 86  baker's yeast
1LLU_A         8 KAAVvhaygaplrIEEVKVPlpg--pgQVLVKIEASGVCHTDLHAaegdwpvkpplPFIPGHEGVGYVAAVGsgvt-rvk 84  Pseudomonas aer...
1LLU_H         8 KAAVvhaygaplrIEEVKVPlpg--pgQVLVKIEASGVCHTDLHAaegdwpvkpplPFIPGHEGVGYVAAVGsgvt-rvk 84  Pseudomonas aer...
1UUF_A        26 VGAYsakq--plePMDITRRepg--pnDVKIEIAYCGVCHSDLHQvrsew-agtvyPCVPGHEIVGRVVAVGdqve-kya 99  Escherichia coli
1YQX_B        20 WAARdqsg--hlsPFNFSRRatg--eeDVRFKVLYCGVCHSDLHSikndw-gfsmyPLVPGHEIVGEVTEVGskvk-kvn 93  quaking aspen
2HCY_A         7 KGVIfyes--hgkLEYKDIPvpkpkanELLINVKYSGVCHTDLHAwhgdwplpvklPLVGGHEGAGVVVGMGenvk-gwk 83  baker's yeast
NP_925782      2 KAAVvpnlhgrweIRDLPIPepg--inQVLIRIKASGLCYTDIHItegaw-padsfPRTLGHEPVGEIVALGagvr-trr 77  Gloeobacter vio...
YP_368599      4 KAIVmtganrpweVQEVAVPvag--pgQVLVKVHASGMCYTDVWAtqgfg--gdiyPQTPGHEVVGEIVEVGagvh-arr 78  Burkholderia sp...
YP_642849      2 RAMVlrapggplrPEELPVPepg--sgEVLVRVRACGVCRTDLHVvdgel-repklPLVVGHQIVGVVERVGegve-rfs 77  Rubrobacter xyl...
NP_396172      2 KAVVmtganqpweVQEVPVPkae--pgLVLVKIHASGMCYTDVWAtqgag--gdiyPQTPGHEVVGEIIEVGagvh-trk 76  Agrobacterium t...
ABY66028       2 KAAVipkpdatweLREVETPrpg--pgEVLIRVRACGVCHNDVLAtrgaipfpsisPAITGHEPAGEVVELGsgvv-rrr 78  Actinomadura ma...
YP_001822133   2 KAAVipavnarweIQEVPTPrpg--pgEVLVKVHACGLCHNDLWTaqgvfpfpscdPAITGHEAAGEVVELGpgtt-tre 78  Streptomyces gr...
YP_001889385   5 KAVQvakaggpleLVERDVPepg--agQVLIKIQACGICHSDVLTkegqw-pgleyPRVPGHEIAGVIDTVGagve-gwa 80  Burkholderia ph...
Feature 1                                                                                        
1PS0_A        87 VGQRVGVGAqvfsCLECDRCkn------dnEPYCTkfvttysq-pyedgyvsQGGYANYVRVhehFVVPIPEn-iPSHLA 158 baker's yeast
1LLU_A        85 EGDRVGIPWlytaCGCCEHClt------gwETLCEsqqnt--------gysvNGGYAEYVLAdpnYVGILPKn-vEFAEI 149 Pseudomonas aer...
1LLU_H        85 EGDRVGIPWlytaCGCCEHClt------gwETLCEsqqnt--------gysvNGGYAEYVLAdpnYVGILPKn-vEFAEI 149 Pseudomonas aer...
1UUF_A       100 PGDLVGVGCivdsCKHCEECed------glENYCDhmtgtynsptpdepghtLGGYSQQIVVherYVLRIRHpqeQLAAV 173 Escherichia coli
1YQX_B        94 VGDKVGVGClvgaCHSCESCan------dlENYCPkmiltyas-iyhdgtitYGGYSNHMVAnerYIIRFPDn-mPLDGG 165 quaking aspen
2HCY_A        84 IGDYAGIKWlngsCMACEYCel------gnESNCPhadls--------gythDGSFQQYATAdavQAAHIPQg-tDLAQV 148 baker's yeast
NP_925782     78 LGDRVGVPWlqlaCGRCEWCrr------gkALFCKdqqgt--------gvqtQGGHAEYMLAqaeATVLLPDa-lAYEQA 142 Gloeobacter vio...
YP_368599     79 VGDRIGTTWvqsaCGRCPYCre------hrPLSGQtavncdap--rttgfasQGGHAEYIAVaaeGTVLLPDa-lAYVDA 149 Burkholderia sp...
YP_642849     78 PGDRVGVPWlgrtCGRCRYCrt------grENLCGearft--------gyqlDGGFAEYARAdarFCLPVPEg-yPDLQA 142 Rubrobacter xyl...
NP_396172     77 VGDRVGTTWvqssCGRCSYCrq------nrPLTGQtamncdsp--rttgfatQGGHAEYIAIsaeGTVLLPDg-lDYTDA 147 Agrobacterium t...
ABY66028      79 VGDRVGTTWvragCGTCAYCrr------dlPVTGQtamncpnp--vttgftvQGGHAEYMAVreeETVLLPDe-lPFELA 149 Actinomadura ma...
YP_001822133  79 VGDRVGTTWvqatCGRCAYCrrnlpltgqsAMSCAapvmt--------gltvQGGHAEYLCVaaaSTVLLPDg-lDYATA 149 Streptomyces gr...
YP_001889385  81 AGQRVGVGWhgghCGRCEHCrr------gdFVLCQralvp--------gisyDGGYAEFMVApveALARIPDd-lSDVDA 145 Burkholderia ph...
Feature 1            #   #                    # ## #                 ###     #                   
1PS0_A       159 APlLCGGLTVYSPLvrngc-gpgkkVGIVGLGG-IGSMGTLISKAMGaETYVISRSs--RKREDAMKmg----------a 224 baker's yeast
1LLU_A       150 APiLCAGVTVYKGLkqtna-rpgqwVAISGIGG-LGHVAVQYARAMGlHVAAIDIDd--AKLELARKlg----------a 215 Pseudomonas aer...
1LLU_H       150 APiLCAGVTVYKGLkqtna-rpgqwVAISGIGG-LGHVAVQYARAMGlHVAAIDIDd--AKLELARKlg----------a 215 Pseudomonas aer...
1UUF_A       174 APlLCAGITTYSPLrhwqa-gpgkkVGVVGIGG-LGHMGIKLAHAMGaHVVAFTTSe--AKREAAKAlg----------a 239 Escherichia coli
1YQX_B       166 APlLCAGITVYSPLkyfgldepgkhIGIVGLGG-LGHVAVKFAKAFGsKVTVISTSp--SKKEEALKnfg---------a 233 quaking aspen
2HCY_A       149 APiLCAGITVYKALksanl-maghwVAISGAAGgLGSLAVQYAKAMGyRVLGIDGGe--GKEELFRSig----------g 215 baker's yeast
NP_925782    143 ASlFCAGYTVWAGLrfadp-kpherVAVLGIGG-LGHLAVQYGKAAGfETFAITHSp--SKEKLVRSlg----------a 208 Gloeobacter vio...
YP_368599    150 APmMCAGYTTWSGLrdaap-qphekVAVLGIGG-LGHVALQLSRACGfETIAITHSa--DKRDLAIGlg----------a 215 Burkholderia sp...
YP_642849    143 APlLCAGLIGYRALrmag---daerLGLYGFGA-SAHIILQVARHQGrRVFAFTRDgdeEAQRFARRlgaewaggsgqrp 218 Rubrobacter xyl...
NP_396172    148 APmMCAGYTTWSGLrdaep-kpgdrIAVLGIGG-LGHVAVQFSKALGfETIAITHSp--DKHKLATDlg----------a 213 Agrobacterium t...
ABY66028     150 APvLCAGYTAWSALrtaep-apherVAVLGIGG-LGHLALQFSRACGfETIAITSSp--DKYDLARElg----------a 215 Actinomadura ma...
YP_001822133 150 APvLCAGYTAWSALrvsep-rpherIAVLGIGG-LGHLAVQYSRACGyETVAITRSa--EKHEVSTRlg----------a 215 Streptomyces gr...
YP_001889385 146 APlLCAGITTFNALrnsga-ragdvVAVLGIGG-LGHLGVQFARKMGfVTVAIARGq--DKASLAKElg----------a 211 Burkholderia ph...
Feature 1                                     ####                     ##                        
1PS0_A       225 dHYIATleegdwge----kyfdtfdlIVVCASSltdidFNIMPKAMkvgGRIVSISipeqhemls----lkpyglkaVSI 296 baker's yeast
1LLU_A       216 sLTVNArqedpveai--qrdiggahgVLVTAVSns--aFGQAIGMArrgGTIALVGlppgdfptp----ifdvvlkgLHI 287 Pseudomonas aer...
1LLU_H       216 sLTVNArqedpveai--qrdiggahgVLVTAVSns--aFGQAIGMArrgGTIALVGlppgdfptp----ifdvvlkgLHI 287 Pseudomonas aer...
1UUF_A       240 dEVVNSrnademaa-----hlksfdfILNTVAAph--nLDDFTTLLkrdGTMTLVGapatphksp---evfnlimkrRAI 309 Escherichia coli
1YQX_B       234 dSFLVSrdqeqmqa-----aagtldgIIDTVSAvh--pLLPLFGLLkshGKLILVGapekplelp----afsliagrKIV 302 quaking aspen
2HCY_A       216 eVFIDFtkekdivgavlkatdggahgVINVSVSea--aIEASTRYVranGTTVLVGmpagakccs---dvfnqvvksISI 290 baker's yeast
NP_925782    209 dGVFPSgaalre--------aggadvILACSNSyr--aTGEALQGLrpdGRLVLMGlpsdnqtla---vsgdllvnrWRI 275 Gloeobacter vio...
YP_368599    216 dRVVENgaalrd--------lggadvLLVTTNEfs--tAEEAMTGVrvdGRVILCGldfskpfsia-sqrkpfhmmrQQV 284 Burkholderia sp...
YP_642849    219 pEELDA--------------------AIIFAPVga--lVPLALRAVargGTVVCAGihmsdipsf----pyellwgeRQL 272 Rubrobacter xyl...
NP_396172    214 dIVVADgkelle--------aggadvLLVTTNDfd--tAEKAMAGVrpdGRIVLCAldfskpfsip-sdgkpfhmmrQRV 282 Agrobacterium t...
ABY66028     216 dIVVRDgaqlca--------aggadvVIATVPShp--aAAAGLQGLragGRMVLAGidaaepftippamtfpffaqgQRI 285 Actinomadura ma...
YP_001822133 216 dLVVRDgeelre--------aggadvVLVTGTSye--aATDALQGLrtgGRIVLATidpagsftid--patpfwargQRV 283 Streptomyces gr...
YP_001889385 212 hHYIDSttanvaqal---qalggarvILATVTSgk--aMSAVVGGLglnGKLIMVGlseepvevp----iaqfimgrNSV 282 Burkholderia ph...
Feature 1          ###                                                  
1PS0_A       297 SYSALGSiKELNQLLKLVSEKdikiwVETLpvgeagVHEAFERMEkgdvryRFTL 351 baker's yeast
1LLU_A       288 AGSIVGTrADLQEALDFAGEGlvkatIHPGkl--ddINQILDQMRagqiegRIVL 340 Pseudomonas aeruginosa
1LLU_H       288 AGSIVGTrADLQEALDFAGEGlvkatIHPGkl--ddINQILDQMRagqiegRIVL 340 Pseudomonas aeruginosa
1UUF_A       310 AGSMIGGiPETQEMLDFCAEHgivadIEMIra--dqINEAYERMLrgdvkyRFVI 362 Escherichia coli
1YQX_B       303 AGSGIGGmKETQEMIDFAAKHnitadIEVIst--dyLNTAMERLAkndvryRFVI 355 quaking aspen
2HCY_A       291 VGSYVGNrADTREALDFFARGlvkspIKVVgl--stLPEIYEKMEkgqivgRYVV 343 baker's yeast
NP_925782    276 IGSQQNGpEHLFEALDYAAKGkvkvvAETYsl--deINLAFDRVFsgdvrfRAVI 328 Gloeobacter violaceus PCC 7421
YP_368599    285 IGSTHGGlRYLSEVLDLAAKGkvkpiVETFsl--dqATEAYDRLAsgkmrfRGVF 337 Burkholderia sp. 383
YP_642849    273 RSVANLTrRDGEEFLALAPEVpv--rTEVRpyplegANQALDDLRs----gRLTG 321 Rubrobacter xylanophilus DSM 9941
NP_396172    283 VGSTHGGqHYLAEILDLAAKGkvkpiVETFal--eqATEAYERLStgkmrfRGVF 335 Agrobacterium tumefaciens str. C58
ABY66028     286 MGATHGGpVLLREALDLVAERkvtpmVETFpk--eqVAEAVEKTAkgelrfRAVV 338 Actinomadura madurae
YP_001822133 284 LGATHHGlRYLTEALDLVAEGavtpmTEVFpm--daVADAVDKVEkgevrfRAVV 336 Streptomyces griseus subsp. griseus NBRC...
YP_001889385 283 QGWPSGTsADSQDTLAFSALSgikpmIEEFpl--tkAAEAYDRMMsgaarfRVVL 335 Burkholderia phytofirmans PsJN

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