Conserved Protein Domain Family
MDR_enoyl_red

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cd08244: MDR_enoyl_red 
Possible enoyl reductase
Member identified as possible enoyl reductase of the MDR family. 2-enoyl thioester reductase (ETR) catalyzes the NADPH-dependent dependent conversion of trans-2-enoyl acyl carrier protein/coenzyme A (ACP/CoA) to acyl-(ACP/CoA) in fatty acid synthesis. 2-enoyl thioester reductase activity has been linked in Candida tropicalis as essential in maintaining mitiochondrial respiratory function. This ETR family is a part of the medium chain dehydrogenase/reductase family, but lack the zinc coordination sites characteristic of the alcohol dehydrogenases in this family. NAD(P)(H)-dependent oxidoreductases are the major enzymes in the interconversion of alcohols and aldehydes, or ketones. Alcohol dehydrogenase in the liver converts ethanol and NAD+ to acetaldehyde and NADH, while in yeast and some other microorganisms ADH catalyzes the conversion acetaldehyde to ethanol in alcoholic fermentation. ADH is a member of the medium chain alcohol dehydrogenase family (MDR), which has a NAD(P)(H)-binding domain in a Rossmann fold of a beta-alpha form. The NAD(H)-binding region is comprised of 2 structurally similar halves, each of which contacts a mononucleotide. The N-terminal catalytic domain has a distant homology to GroES. These proteins typically form dimers (typically higher plants, mammals) or tetramers (yeast, bacteria), and have 2 tightly bound zinc atoms per subunit, a catalytic zinc at the active site, and a structural zinc in a lobe of the catalytic domain. NAD(H) binding occurs in the cleft between the catalytic and coenzyme-binding domains at the active site, and coenzyme binding induces a conformational closing of this cleft. Coenzyme binding typically precedes and contributes to substrate binding. Candida tropicalis enoyl thioester reductase (Etr1p) catalyzes the NADPH-dependent reduction of trans-2-enoyl thioesters in mitochondrial fatty acid synthesis. Etr1p forms homodimers, with each subunit containing a nucleotide-binding Rossmann fold domain and a catalytic domain.
Statistics
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PSSM-Id: 176206
View PSSM: cd08244
Aligned: 13 rows
Threshold Bit Score: 312.766
Threshold Setting Gi: 32469247
Created: 4-Sep-2009
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
putative NAD(P)
Feature 1:putative NAD(P) binding site [chemical binding site]
Evidence:
  • Comment:MDR family binds NAD(P) as a cofactor
  • Comment:defined by comparison to MDR alcohol dehydrogenase like family

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                               ###  #                                   
BAC79021       1 MLSVVMEEFGGPeVLRARQVEDPEPGPGQVLVDVAYASVTFVETQVRSGNGpfg--rpaLPRVPGNGVGGRVvALGPGAD 78  Streptomyces sp...
NP_628438      1 MHAIRLHSFGPAgNLVLEEVEDPAPGPGQVRVAVRAAGVHLLDTALREGVQgpsptlpeLPTIPGREVAGLVdALGEGAP 80  Streptomyces co...
YP_001107375   1 MHAIRQHAFGPAeNLRYEEVADPHPGPGQVRIAVAAAGVHLVDTMIRQGVQggpfprpeLPMTPGREVAGVVdEIGADVD 80  Saccharopolyspo...
YP_001105931   6 MRVVWASGFGGPdVLVAGRAEAPVAGPGQVVVAVEAVSITFVETQIRRGESpgpp-lpeAPYVPGNAIAGRVmATGGGVA 84  Saccharopolyspo...
YP_002884089   1 MLAIRHHSFGPPdVLHPEHVADPEPGAGELRIAVEAAGIHLLDTTIRSGADggpaplpeLPTIPGREVAGIVdAVGDGVP 80  Beutenbergia ca...
YP_001160208   1 MRAIQVTAFGGPeVLSPAELPDPAPGPGQVVVALAAADVIFLDTLLRSGWGqdff-prtLPYVPGGGGAGVVvQTGDGVD 79  Salinispora tro...
YP_001508285   1 MRAIRLHAFGPAeNLVCEDVPDLLPAEGQVRIDVQASGVHLIETRLRRGVGvgphlppeLPTTLGGEVAGVVgAVGSGVG 80  Frankia sp. EAN...
YP_716445      1 MYAVRLHEFGPAsNLRHEQVDDPVPAAGQVRIAVAAAGVHLVDTTLRQGWAggpfqrpeLPTIPGREVAGTVdAVGPGVD 80  Frankia alni AC...
NP_825195      1 MRAAQVKEFGGPeVLVPVDLPDPVPGPGEVVIEVAYTDTIFVETQLRSGWGreff-paePPYVPGGGVSGVVgALGPDVP 79  Streptomyces av...
CAI78430       1 MRAVVLHEFGPAaNLRYETVPDPVPGPGEVRLLVKAAGVHQIETAMREGLEigpp-lpvLPAIFGAEVAGVVeAVGPAVD 79  Streptomyces am...
YP_120337      1 MRAVQATRFGGPeVLAVRELPDPVPASGEVLIEVAAADVMFLDTRLRSGWGtgff-pvrPPYVPGGGVGGTVrAVGPGVD 79  Nocardia farcin...
YP_003112465   1 MRAVWLRDFGAPtELHAEDTPEPVPQPNTALVDVAYANITFVETQARAGTGpfh---ptLPTIPGNGVGGVVrAVGDGVD 77  Catenulispora a...
YP_003114026   1 MRVIRVREFGDPsVLKLEDAPPPAAAAGQAVIDVEVAAVLFGDTIVRGGRFpf-----pLPYTPGLEVGGRVaAVGPGGD 75  Catenulispora a...
Feature 1                                                             #     #                    
BAC79021      79 PALLGTVVVTttg--------geGGYAERALAradEVVPVPPGLalkdAVALLADG--RTALLLFRqaevKPGEk-VLVE 147 Streptomyces sp...
NP_628438     81 ESWLGRRVVAhlg-------fapGGYAALAVTdlaRVHEIPANLd--fAEAVAMIGtgRTAMGILQfadlGPGSv-AVVP 150 Streptomyces co...
YP_001107375  81 PVWLGRRVVThlg-------magGGYAELAVReveSLHVLPEDVg--fEAAVAMIGtgRTTMAILRiagmTPDDdvVVVT 151 Saccharopolyspo...
YP_001105931  85 PAWRGKRVVTetv-------ddnGGYAEQAPAaveDLLSVPDGLglpeAAALLHDG--STALGLFDnagiRAGEw-VLVE 154 Saccharopolyspo...
YP_002884089  81 DTWLGRRVVAhlga------grgGGSAELAVVaaaRAYEIPDGLd--aAVAVAAIGtgRTATGILDlaelQAGEv-AVVT 151 Beutenbergia ca...
YP_001160208  80 PAWIGRRVVVqt----------sTGYAERVAAhveEILPVPDGLaieaAAALVHDG--VTALRLDQlgtpRKREw-VLVS 146 Salinispora tro...
YP_001508285  81 TGWLGRRVVAqlg--------agGGYAEQAVAsadSVHVLPEHLdaetAVAVVTNG--ATAQGVLGvanlTTDDv-VLVM 149 Frankia sp. EAN...
YP_716445     81 DGWLGRRVVAhlg-------qagQGYAQLAVRevdNVHPIPPTLd--aPAAVAMIGtgRTAIGILDva-eLTAEdvVLIT 150 Frankia alni AC...
NP_825195     80 AEWLGRRVTSfv----------tGSYAERAVAavsSLTPVPDALdlltAAALVHDG--VTATGLLElttvTETDr-VLIL 146 Streptomyces av...
CAI78430      80 DAWIGADVVTahg--------rpGGYAELAVAdvsSLHRIPAGLrheaAVTMVVTG--ATTMGLLDiaelTSDDv-VLVT 148 Streptomyces am...
YP_120337     80 TAWLGARVVGptaasgigggvptGGYAERALVteqTLLAVPAGLdidrAVALVHDG--RTALAAFDratvQPGDl-VLVT 156 Nocardia farcin...
YP_003112465  78 PALIGTRVVGstg--------gsGGYAEVALVpidVLYPVPDTLaldaAVAVLADG--RTASLLIEaatiRPGDr-VLIE 146 Catenulispora a...
YP_003114026  76 EALVGRRVVAvtp-------rtgGGYAEQAVVdtrWLFEVPDGTalddAVPVFQAG--AVALGMLGamrlTAGEt-ILIT 145 Catenulispora a...
Feature 1        #### ##                 ##   #               #                       ## #       
BAC79021     148 AAAGGVGSLLVQLAVhAGARVVGAARGdrKAELVVSLGA-SYVDYSreAWLRQVRetag-ggdlDVVFDGVGGaiGTAAA 225 Streptomyces sp...
NP_628438    151 AAAGGIGTLLVQYAKnAGATVVGLAGGpdKTALVAANGAdLAVDYLdpQWPAKVRahlg-ggeaTVVFDGVGGdvAREAV 229 Streptomyces co...
YP_001107375 152 SAAGGIGNLLVQRGRtVGATVVGAAGSaaKVEKVRALGAaVAVDYTtpDWTTAVRdqlg-greaTLVLDGAGGryGREAF 230 Saccharopolyspo...
YP_001105931 155 AAAGGLGSLLVQLARaAGARVIGAARGerKLDVVRGLGAeVVVDYSepDWTDRLRdt-----ppTLVFDGVGGaiGRAAF 229 Saccharopolyspo...
YP_002884089 152 SAAGGLGVLLVQAVAgVGGTAVGLARGerKLAVAREAGAaVVVDYTrpDWDDVVRqla---pppTVVFDAVGGdvGRRAY 228 Beutenbergia ca...
YP_001160208 147 AAAGGAGSLLVQLAVnAGARVVAAASTdaKLALARQLGAeIAVDYTrpGWIDQVReatg--ggvALVYDGAGGptGTATL 224 Salinispora tro...
YP_001508285 150 SAAGGLGSLFVQAAHhAGVTVVGVAGGdaRAARARELGAdVAVDYQdaDWPALVReald-gravSVVLDGVGGvlGRQAL 228 Frankia sp. EAN...
YP_716445    151 AAAGGIGSLLVQAARgVGATVVAIAGGavKVAAARRLGAdLAVDYTlaDWPDAVRaald-grtvTVTLDGVGGpaGRAAL 229 Frankia alni AC...
NP_825195    147 GASGGMGTLLVQLAHaRGARVVGVARGeaKVALVRKLGAdEVVDATlaDWPSRARhalggdgaaDVVLDGVGGelGAAAF 226 Streptomyces av...
CAI78430     149 SAAGGIGRLVVQFAGrLGATVIGAAGGpaKTAAVRELGAdVAVDYNlpDWAGAVRdalg-grgvTVVLDGVEGdkGRAAF 227 Streptomyces am...
YP_120337    157 AAAGGLGTLLTQLAHrAGAKVVAAARGaaKLELAHRLGAhLSIDYSepDWAEHARaatg-gsgfHVVFDGVGGalGDAAL 235 Nocardia farcin...
YP_003112465 147 AAAGGVGTLLIQLAKtAGATVIALAGGprKTDLATALGAdQAIDYRdpAWPRQLDep------vDVVFDGVGGatGRTAY 220 Catenulispora a...
YP_003114026 146 AAAGRLGSLLVQAAKaAGATVIGAVGSsgKTAFVTALGAdAVVDYSmpDWIDQVTtatg-gqgvDLALDAVGGaiGAGAL 224 Catenulispora a...
Feature 1                    ##                       ###                                        
BAC79021     226 GALRp-GGRISLYGMASGadatldedDLAARGIRTLGLFAapgpa-----ethpLIGEALRLAADGVLAPVvgQVFPLAA 299 Streptomyces sp...
NP_628438    230 ALLGp-GGRHLVFGWSGKglh--dgtPYVVDGVSQQVLGPvmmeragganpvrtLELRALAEAAEGRLVPAv-HRFPLAE 305 Streptomyces co...
YP_001107375 231 DLLGs-GGRIVMYGRPFGpehrftseDLFDRALSATVGLGpnllkt--pgglraLEEESMTALAGGGLTPLvhAPFPLAE 307 Saccharopolyspo...
YP_001105931 230 ECLAp-GGRFSIHGAASGavtaladgEARRRGVTVLGLEQlfclr----tgmrdRVQRALAEAAAGRLTPTigQTYPLEQ 304 Saccharopolyspo...
YP_002884089 229 ALLAp-GGRLVRYGWTSGeqt--aydDADRRVVDVLGPAMvareg-----glasLESEALARAADGTRVPLvgSTFPLAD 300 Beutenbergia ca...
YP_001160208 225 DAVAd-GGRLVTYGTADGlatp-dpdSAAGRGIRIFAPLLdgppdr---qtvreLMGLALERTTRGRMHPVigATYPLER 299 Salinispora tro...
YP_001508285 229 ELLGp-GGRFLMYGWAAGeptaittrDLVERELTATWAIGpnatps---ggwreLAARALEEAAAGRLVPLm-TRFLLER 303 Frankia sp. EAN...
YP_716445    230 ELIGp-AGRYYTFGWAAGspttitteDVTERGLLVASVLGprmfrr--pgglaaLEEAALAAAAAGTLVPAv-QTFPLAE 305 Frankia alni AC...
NP_825195    227 TLTAd-GGRFSAHGAPSGgfaaldpdEAGRRGITLFGIGDvqfgp----adlsrLTAGALAEAAAGRLRPVvgEVFALEK 301 Streptomyces av...
CAI78430     228 GLLAq-GGRYITIGAASReefrpdeaELKERGVHFTDALAilleg----qdaaaDEARALTAAADGNLVPAf-QAFPLSQ 301 Streptomyces am...
YP_120337    236 AVTAd-GGRFLGYGSASGefagahaeAARARGVEVVGLFDlqagt----pdwraLGAEALRMAAEGHLEVVvgQAFPLDQ 310 Nocardia farcin...
YP_003112465 221 EQLRp-GGRILTYGLASGqwtavpedEARERGVTVVRPTAgpad-------lrrLTESALRAAADGTLRPVigQRFPLER 292 Catenulispora a...
YP_003114026 225 AALRdgAGRLGSYGFASGewtpldagVIGRRGLSVVGAAGitfakp--eaeqraDAERALSELAAGRLIPRihAAFPLEG 302 Catenulispora a...
Feature 1                             
BAC79021     300 AAEAHAAIEARATVGKTLLAV 320 Streptomyces sp. AM-7161
NP_628438    306 AAAAHRALENRGTTGKVVLEP 326 Streptomyces coelicolor A3(2)
YP_001107375 308 AAAAHTALENRRSYGKVVLEP 328 Saccharopolyspora erythraea NRRL 2338
YP_001105931 305 AAEAHAAIESRSVIGKTLLLP 325 Saccharopolyspora erythraea NRRL 2338
YP_002884089 301 AADAHRALEARETHGKVVLLT 321 Beutenbergia cavernae DSM 12333
YP_001160208 300 AAAAHRALAMRTTVGKSLLLV 320 Salinispora tropica CNB-440
YP_001508285 304 AADAHSALEAREAEGKVVLTI 324 Frankia sp. EAN1pec
YP_716445    306 AAAAHAALEGRATTGKVVLLP 326 Frankia alni ACN14a
NP_825195    302 AAEAHAAIEGRQSAGKVLLRR 322 Streptomyces avermitilis MA-4680
CAI78430     302 AAEAHAALEARATTGKVILVP 322 Streptomyces ambofaciens ATCC 23877
YP_120337    311 AAGAHAAIETRTAVGRTVLIP 331 Nocardia farcinica IFM 10152
YP_003112465 293 ADAAHAAIEARSTVGKTLLLT 313 Catenulispora acidiphila DSM 44928
YP_003114026 303 APEAHAELEARSTIGAILLRP 323 Catenulispora acidiphila DSM 44928

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