Conserved Protein Domain Family
THR_DH_like

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cd08239: THR_DH_like 
L-threonine dehydrogenase (TDH)-like
MDR/AHD-like proteins, including a protein annotated as a threonine dehydrogenase. L-threonine dehydrogenase (TDH) catalyzes the zinc-dependent formation of 2-amino-3-ketobutyrate from L-threonine via NAD(H)-dependent oxidation. The zinc-dependent alcohol dehydrogenases (ADHs) catalyze the NAD(P)(H)-dependent interconversion of alcohols to aldehydes or ketones. Zinc-dependent ADHs are medium chain dehydrogenase/reductase type proteins (MDRs) and have a NAD(P)(H)-binding domain in a Rossmann fold of an beta-alpha form. The N-terminal region typically has an all-beta catalytic domain. In addition to alcohol dehydrogenases, this group includes quinone reductase, sorbitol dehydrogenase, formaldehyde dehydrogenase, butanediol DH, ketose reductase, cinnamyl reductase, and numerous others. These proteins typically form dimers (typically higher plants, mammals) or tetramers (yeast, bacteria), and generally have 2 tightly bound zinc atoms per subunit. The active site zinc is coordinated by a histidine, two cysteines, and a water molecule. The second zinc seems to play a structural role, affects subunit interactions, and is typically coordinated by 4 cysteines.
Statistics
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PSSM-Id: 176201
View PSSM: cd08239
Aligned: 14 rows
Threshold Bit Score: 373.193
Threshold Setting Gi: 229820753
Created: 19-May-2009
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
putative NAD(P)catalytic Zn
Feature 1:putative NAD(P) binding site [chemical binding site]
Evidence:
  • Comment:MDR family binds NAD(P) as a cofactor
  • Comment:defined by comparison to MDR alcohol dehydrogenase like family

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                            ###  #                                      
P77360         4 MLAAYLPGNSTVDLREVAVPtPGINQVLIKMKSSGICGSDVHYIYHqhrataaa---pdkplyqGFINGHEPCGQIVAMG 80  Escherichia col...
YP_001179225   4 MFGVVFPGDKKVEVREFPIPkPKDGEVLVKIKASAICRSDMSLYYGnpvvgg------eiaksgSIIPGHEPAGEIVEVG 77  Caldicellulosir...
YP_001862910   1 MRGIVFRGDCTLELATFPDPtPGVGEVVLEIKASGMCGSDLHFYRRpags-----------svpPVIAGHEPCGIVVAVG 69  Burkholderia ph...
YP_296034      1 MRGMIFRGAREVELAHFDDPtPGPGEAVIEMKASGMCGTDLHYYRHgmaaslemlglkdritsdRIIGGHEPCGVVAALG 80  Ralstonia eutro...
YP_771203      1 MKGLVFLGEGKMEFRDYPDPvPGPDEVVVRIKASGMCGSDLNHLNEpkrr-----------edqIVIEGHEPCGIVEEVG 69  Rhizobium legum...
YP_002764294   2 MQGLVFEGDRQISLRDFSDPnPGPGEVLLRVTASGMCGSDLHYYRAeasa----------pdapVCIGGHEPAGVIEELG 71  Rhodococcus ery...
YP_831311      5 VRALRFAEKDRVSVVDAALPeAGPGEIVLDVKAAGLCGSDLHYFHMthnqmqn----atqprspEMTPGHEIAGVVASIG 80  Arthrobacter sp...
YP_003321871   1 MRGVVLPGNRTVDLREFPTPqPGYGQVLVRMKASGICGSDIRAIYRewlgtg-------peaykGCIAGHEPCGIVEEVG 73  Thermobaculum t...
YP_003012304  11 MNIPLFVGQGKIEYGEKPVPlPGKGQLLLQVKANALCGSDRSQFHDg----------------sTTVPGHEAAGIVVGAG 74  Paenibacillus s...
YP_002882279   4 SEAPRLVGDERIDVVPISYPdPGAGQLLLRVRANALCGSDRGAWAHg----------------aDVVPGHELAGEVVAAG 67  Beutenbergia ca...
Feature 1                                                                                        
P77360        81 QGCr--HFKEGDRVLVYHISGCGFCPNCRrGFPISCTGEGka--aYGWQRDGGHAEYLLAEEKDLILLPDaLSYEDGAFI 156 Escherichia col...
YP_001179225  78 KGVt--NVKPGDRVAIYLAVSCGECLYCKsGYKMMCKQFKc----IGFDLHGGDAEYMVVPAENCMKIPDeMSYITAAVS 151 Caldicellulosir...
YP_001862910  70 PGTvgpHAHVGARVMVHHYSGCMGCPDCRsGWPQMCRTAPit--vYGTDAHGGHAPFMKVPADTLVPLDErLSFEAGAAI 147 Burkholderia ph...
YP_296034     81 PGVdskAFRVGDRVMVFHYEGCRHCDHCRtGWTQLCDHGAr---iHGVLAHGGHADYMKVPVSSLVHLPQeMSFAGGAAV 157 Ralstonia eutro...
YP_771203     70 SAVrpwEARVGDRVMVYHYDGCRTCPNCRtGWMQNCDNTRi---aFGGNGHGAHAPFMKVPAHTIIKLPEqLSFNAGAAV 146 Rhizobium legum...
YP_002764294  72 AGVdpvRFAVGQRVMVHHYAGCGGCEACRsGWTQMCTTGPat--vYGKSAHGAHAPFMTVSTQSVLPLSDsLSDIAAAAI 149 Rhodococcus ery...
YP_831311     81 PGVt--HPSVGDRVAIQHYSGCGTCETCRkGWDVHCENARv----YSLNRPGGCQDQVIVTAKDAVVLPDsVSFATGAFL 154 Arthrobacter sp...
YP_003321871  74 PGVe--HFSPGDRVVVYHIVGCGVCKDCRaGWMINCKAPYpyraaYGWQRDGGHADFLLAEQRTLIKLPDnLTFVDGALA 151 Thermobaculum t...
YP_003012304  75 DGA---TTVIGTPGVVFLMDFCGECRSCKlGFTNQCLNKRa---dYGFTHDGGYTPYIVVNENVFFPIDPsIPLADATML 148 Paenibacillus s...
YP_002882279  68 PET---RTSVGTRGVVYLMVFCGRCRNCRrGMTNLCLDKQg---dVGFNRDGGLGPFVLVEERVFFAVDDdVPFALATML 141 Beutenbergia ca...
Feature 1         #   #                     ######                    ##   #                 #   
P77360       157 SCGVGTAYEGILRGEVsg--sDNVLVVGLGPVGMMAMMLAKGRGAk--rIIGVDMlpERLAMAKQLGvmdhgYLATTegl 232 Escherichia col...
YP_001179225 152 TDAIGTLYHAQKRMGIsg--rDIVAIYGIGPMGAAGILVAKGLGAt---VIAVDTidKRLDWAKELGa---dYVINSqst 223 Caldicellulosir...
YP_001862910 148 SCGTGTAWGAFERIGLtg--rDTLAVFGQGPVGLSATMLAKAQGAr---VIALDIdeSRRKTAKEFGa---dAVINPldg 219 Burkholderia ph...
YP_296034    158 ACGTGTAYGALLRLDIsa--rDTLAVYGLGPVGLSVAQLAAAMGVe---VFGVDIsaARVTRAKDFGv---tHAIDAtqs 229 Ralstonia eutro...
YP_771203    147 GCGSGTAYAALKRVNLla--dETIAIFGQGPVGLSCTVFAKAFGAr---VISLDVgeERLQMAKDFGa---dFVINPmkd 218 Rhizobium legum...
YP_002764294 150 GCGTGTAWGALDRVGDvg--gADLVVFGQGPVGLSATMLATARGAr---VIAVDLadSRLEQARAFGa---aETINSgsv 221 Rhodococcus ery...
YP_831311    155 ACGATTAYQAIQRSEVrp--gGTVLVIGAGPVGLAVLTWARALGLh---AIATDPspQRRKFASKIGf---tDVYDSnn- 225 Arthrobacter sp...
YP_003321871 152 ACGLGTGYQAILRAGVsg--rDRVAVVGLGPVGLGILMLARASGAe---VIGIDVipERLEMAQKIGa---eHTFLSgdn 223 Thermobaculum t...
YP_003012304 149 LDIMGTGGHAIKRAKLvhpdvQSVLIAGAGPIGLGVLAMAKLLLGeetpVFIMDYvdYRLELAEKMGa---iPVQLAdq- 224 Paenibacillus s...
YP_002882279 142 LDVMGTSGHALDRAERllpdvGSIHVVGAGPVGLGTLVMARIRYGddvpIAVSDVspERLGLAASLG-----ATVAVa-- 214 Beutenbergia ca...
Feature 1                               ## #                   ##                       ###      
P77360       233 --pqiIAELTHG----GADVALDCSGNaaGRLLALQSTadWGRVVYIGETGkveFEVSADLMh-hQRRIIGSWVTslfHM 305 Escherichia col...
YP_001179225 224 davqeILKITGD----GADAAIDCSGNpnAENNALNCVrpHGRVAFIGENSkttIDPSNQFIr-kQISLIGSWYFpiwEY 298 Caldicellulosir...
YP_001862910 220 svaeaIRDLTGGk---GASVSVETSGAaaANRDALDSVspWGTVCLVGIGSefsLTLAKYLRt--QVRILTSWTMsvqGQ 294 Burkholderia ph...
YP_296034    230 dpvaeILKLTGGk---GASCAVECAGGelARAQAVRSTrvWGRIALVAVGGnlvLDAMKDLIg-kQRQLLGSFTFsevDM 305 Ralstonia eutro...
YP_771203    219 dpvkaIRDLTKGg--lGADKAIECSSNsvARQQALQAIkrWGTTCLVGVHGs-mQFECNELIt-nQKNLVGSLTFnknLQ 294 Rhizobium legum...
YP_002764294 222 dpvsmIKQLTSG----GATVAIETSGAtaAATAALESLapWGRLGVVGLGGr--IEVDVRKFlsrQVTIATSWSLssvQQ 295 Rhodococcus ery...
YP_831311    226 ---fdISTLRAE----RADVVIETSGNihGRRAALEGVrtWGTVVFVGLGPgfdIDPVPDLIm-rQVTLRGMFVFsvpLM 297 Arthrobacter sp...
YP_003321871 224 t-aerIMDLTKGe---GVEVVIDASGNplGRHLCLEIAgeWGRVVFVGEGNtvsFEPSPLLIh-kQLTLMGSWVCglvQM 298 Thermobaculum t...
YP_003012304 225 ----sVQEVIKAagleGADIAIDTSGKtsGRRTSLDALnqRGVLVCVGHGQelnLGVSNDVIa-pERAVLGSEYFqfnEL 299 Paenibacillus s...
YP_002882279 215 -----PHEVGSLe---PVDLAVDSSGRteARAAALAGLrkPGVLVCVGHGQelhLDVSRDLIa-tERAVIGSEYFpygDL 285 Beutenbergia ca...
Feature 1                                                      
P77360       306 EKCAHDLTDWklWPRNAITHRFSLEQAGDAYALMASGkcGKVVINF 351 Escherichia coli ED1a
YP_001179225 299 EEIANFIIRKkiPAEKLVTHTFKLKDAETAFGLFDNKetGKVVFVE 344 Caldicellulosiruptor saccharolyticus DSM 8903
YP_001862910 295 RACADFVVERglNLDALFTDRWRLDQAEQAYQAFNRQsgGKGVFLM 340 Burkholderia phymatum STM815
YP_296034    306 KACASFIATRgiEVDRLFTDTWRLDQAAQAYAHFDQQagGKGVFLF 351 Ralstonia eutropha JMP134
YP_771203    295 DECAGFVAERgiDVDALFTHEFQLDQAVEAYALFEQRkiGKGVFVF 340 Rhizobium leguminosarum bv. viciae 3841
YP_002764294 296 IQCANFVDRHhlPVDDLFSHRWTLADAVEAYAEFDKQtsGKGVFTF 341 Rhodococcus erythropolis PR4
YP_831311    298 MEAVREVEKHsvDLDPIITRISNIEDGPAAFADFANGsvGKTVIGW 343 Arthrobacter sp. FB24
YP_003321871 299 EELLGFLSRKelHPEQIVTHKFPLEDADEAYRLFDSGksGKVVITW 344 Thermobaculum terrenum ATCC BAA-798
YP_003012304 300 AENHALILKHlpYLQQIITHRYGLDQIQEAYELFFQGntGKVLIEQ 345 Paenibacillus sp. JDR-2
YP_002882279 286 ERNLELLRDHrdAVARVITHTFDVAELAGAFTTFLGGgsGKVVVTQ 331 Beutenbergia cavernae DSM 12333

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