Conserved Protein Domain Family
sugar_DH

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cd08236: sugar_DH 
NAD(P)-dependent sugar dehydrogenases
This group contains proteins identified as sorbitol dehydrogenases and other sugar dehydrogenases of the medium-chain dehydrogenase/reductase family (MDR), which includes zinc-dependent alcohol dehydrogenase and related proteins. Sorbitol and aldose reductase are NAD(+) binding proteins of the polyol pathway, which interconverts glucose and fructose. Sorbitol dehydrogenase is tetrameric and has a single catalytic zinc per subunit. NAD(P)(H)-dependent oxidoreductases are the major enzymes in the interconversion of alcohols and aldehydes, or ketones. Related proteins include threonine dehydrogenase, formaldehyde dehydrogenase, and butanediol dehydrogenase. The medium chain alcohol dehydrogenase family (MDR) has a NAD(P)(H)-binding domain in a Rossmann fold of a beta-alpha form. The N-terminal region typically has an all-beta catalytic domain. These proteins typically form dimers (typically higher plants, mammals) or tetramers (yeast, bacteria), and have 2 tightly bound zinc atoms per subunit. Horse liver alcohol dehydrogenase is a dimeric enzyme and each subunit has two domains. The NAD binding domain is in a Rossmann fold and the catalytic domain contains a zinc ion to which substrates bind. There is a cleft between the domains that closes upon formation of the ternary complex.
Statistics
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PSSM-Id: 176198
View PSSM: cd08236
Aligned: 37 rows
Threshold Bit Score: 343.825
Threshold Setting Gi: 67906772
Created: 29-Apr-2009
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
putative NAD(P)catalytic Zn
Feature 1:putative NAD(P) binding site [chemical binding site]
Evidence:
  • Comment:MDR family binds NAD(P) as a cofactor
  • Comment:defined by comparison to MDR alcohol dehydrogenase like family

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                              ###  #                                    
YP_177063      1 MKALVLEan--qSFTIKDIetpvpkeDEALIKVAYCGICGSDLARYFdgk-vhnYPIVLGHEFSGTVEAVGasvd----s 73  Bacillus clausi...
YP_828728      1 MNALLLTqy--mHLEMVEMpvpaigaDDVLVRVRACGICGSDVHGLDgktgrriPPLVMGHEAAGEVVETGanvt----d 74  Solibacter usit...
NP_866984      1 MKALLLTey--kNMQVTDVdepevgpDDVLVQVEACGICGSDIHGYDgstgrriPPLVMGHEAAGVVVQVGenvr----d 74  Pirellula sp. 1
EDY81991       1 MKALVLEey--gRFEYKEVarpeigpKEVLVKVEVCGICGSDVHGMDgstgrriPPIIMGHEASGEIVEVGaeve----s 74  Verrucomicrobia...
YP_003322359   1 MKALVWQgpyslEIVEKDVpkp--dpGFALVKVEACGICGSDIHGYTgesgrrtPGMIMGHEFAGTVTEVNgear----d 74  Thermobaculum t...
YP_001430942   6 MAALVWLgprkmELRHEPVptp--gpGEVLVAVAAVGICGSELSGYLgqnslrrPPLIMGHEAAGRIAFDYdtplsdgsp 83  Roseiflexus cas...
YP_293272      1 MKAIVYTqpdavEVLDRDMpdl--epGEILLRIDASGICGSDLHAYHghdprrkPGLVLGHEFAGTVIASSdea-----r 73  Ralstonia eutro...
AAY82835       1 MKALVYTgtnasEIRDVEVpea--gaGQCIVDVTFCGICGSDMHAWHghderriPPLVLGHEAVGIARSGLh-------- 70  uncultured bact...
YP_995569      1 MQALVYTqp--qELQLQQRprpelvaGEVLLKIDAVGICGSDLHAWHghdprrkPGLELGHEFVGTVVESAagg------ 72  Verminephrobact...
YP_001540012   1 MRALVWTav--gRMEIKDVpkpipqpGWVVMRVKYTGVCGSDIGGFLgknelrrPPLIMGHEFTGIVEEAGpgvp----k 74  Caldivirga maqu...
Feature 1                                                                                  #   # 
YP_177063     74 vaVGDRVVVAPLLPNKDDPFMqkGSPALSSSYSFLGsr-----enGAMAEYVVVKAeNLLVLPEtvTLAEAALIEPLTVA 148 Bacillus clausi...
YP_828728     75 lrPGDRVTFDSTVYCGRCFHCtrGEVNLCDNREVLGvspgpyrrhGAFAEYVSVPRrIMYRLPDslSYEQAALIEAVSVA 154 Solibacter usit...
NP_866984     75 leLGARVTFDSMVSCGNCEFCreGHGNLCDNRMVLGvscgdyrrhGAFAERISVPRrIVYRLPDtlPFEHAALVEAVSVA 154 Pirellula sp. 1
EDY81991      75 wsIGNRVTFDSTVYRLDDWYTkrGLYNLSDKREVLGvspgdfrrhGAFAEFVSIPEhILHTIPDsvTYEQAAMVEPIAVA 154 Verrucomicrobia...
YP_003322359  75 vaVGQRVAVNPLWGCGKCFYClrGDPQICPNRLTIGvni---tesGAFSEYVTVPVsNLVPIADhvSFEEASTAEPLAVA 151 Thermobaculum t...
YP_001430942  84 arAGARVTFNPLLTCGVCDLCraGRSNLCRNRQLISah-----rpGAFAEYVSVPArLCVPLPDhvSLTLGSLTEPLACS 158 Roseiflexus cas...
YP_293272     74 mpVGQRVTANPLVTCGHCDYClqGRDNLCANRGMVGmn-----rpGALSEYLAIPIrCAIPIPDtlPSVSAALTEPAATA 148 Ralstonia eutro...
AAY82835      71 --AGARVAINPLISCGKCRACasGDRHLCTSRELIGmr-----ypGAFAEQVMIPEsNLTRLDDhlSFQDAALAEPLAVA 143 uncultured bact...
YP_995569     73 faPGTRFTGNPLMACGVCDYCvqGRNNLCAGRTMIGmt-----rpGAYAQYMSIPAaALIAIPQqmSDIAAALSEPAATA 147 Verminephrobact...
YP_001540012  75 eyIGRLFTVNPIIGCGHCRYCrsGLKNLCVMRKIIGid-----ypGAYAEYVAVPV-DNLYPVS--DPVKGALAEPLATS 146 Caldivirga maqu...
Feature 1                               ######                    ##    #              #         
YP_177063    149 IHGIERiv-----lpAGEHVCVLGAGTIGIMTILALKARGAg--kVTAIDInd-eKLAFAKKIGaDDTINSlked--pvs 218 Bacillus clausi...
YP_828728    155 VHAVNLtp-----vrLGDSAVVVGSGMIGLLAMQAAKHAGCt--rVIAVDPde-gRLRLAIGAGaTDAINPkstdaaaai 226 Solibacter usit...
NP_866984    155 VHAAEVtp-----ikLGDTAVVVGAGMIGLLAVQAVRAAGAt--qVIAVDLnd-kRLETAGTLGaDVLLRAdqvdvpekv 226 Pirellula sp. 1
EDY81991     155 LHALRLae-----vdPSQRCVVVGVGMIGVFVLQALRALGVe--tVYAVDLns-rNRERAMKQGaAEAFDPaeeglveri 226 Verrucomicrobia...
YP_003322359 152 LRGFHLsg-----lsQNDVTLICGAGTIGLCALLVCKAHGLe--tVFITDKls-hRLEVAEKIG-GISVAPednp-aerv 221 Thermobaculum t...
YP_001430942 159 VRAVAHa-------gAPRRLVILGAGPIGLLCLVAARAAGIe--sILVSDVsd-rRLAVARAWGaTATVNArghv-laav 227 Roseiflexus cas...
YP_293272    149 LHAVNLtmramsrplPEVSVLIIGGGAIGMLTAALVTSYGCk--rVCVAETnalrQASVSRHLG-CEVIDPt-------v 218 Ralstonia eutro...
AAY82835     144 VRTVGLaksg--gatRDSRSVILGGGAIGLLCAKVMKAGGYapprIAETNSl---RRAMLEGLGlGDCYDPr-------d 211 uncultured bact...
YP_995569    148 WHALHRsmqmltrplHECRVLVIGGGAIGMLAGLLLKRWGVv--rLTLSEPnplrRSAVQTHVG-CATLDP--------- 215 Verminephrobact...
YP_001540012 147 LRAVRLsg-----vsLGDSVLVIGAGPVGSLAIKLLNIGGIk--dITAIDIna-nRLEWARRWGaSKTLSTtgeealkti 218 Caldivirga maqu...
Feature 1                       ## #                   ##                         ###            
YP_177063    219 YASKHGk--pGAVIETAGVpsSQEQAVALVEKkGKIVFVGTasgdvtfpaatFEKILREELEITGSWMsysgpfpgyEWQ 296 Bacillus clausi...
YP_828728    227 RELTEGrg-aDLAIECVGAtdPIRTAIAGVRKgGAVTLVGNvape---iefpLQSVVTRQIRLQGSCAsn------gEYP 296 Solibacter usit...
NP_866984    227 RELTGGrg-aDVALEVVGAtpTVKTAIESVRKgGAVTLVGNvspt---ielpLQSVVTREIRLQGTCGcn------gEYP 296 Pirellula sp. 1
EDY81991     227 QGMTEGrg-vDLAIEAVGIepTVNLAVDLLRKgGTAVLVGNlapd---vsfpLQKVVTRQLRIQGSCAic------gEYP 296 Verrucomicrobia...
YP_003322359 222 LSSTSGlg-aKATLDAVGIgaTITQSLMATMRgGKVVLIGLatpk---vdiaLYELVPQERLIQGSYAyt-----pdEYR 292 Thermobaculum t...
YP_001430942 228 QEFAPGg--aDAAIDAVGLtvTREQAVRAVIPgGRVVFIGLheee---smvaANYIVRQEITVTGSFTys-----daDFT 297 Roseiflexus cas...
YP_293272    219 SPPPESa--fDVVIDAVGAapTRNLAIAATRPgGVVMHIGLqaws---seidMRKLTLAELTLLGTYTyt-----msDMH 288 Ralstonia eutro...
AAY82835     212 GGPDEGs--vDLVIDAVGMgaTRAASSAMVRPgGVIVHVGLqdne---pgldTRRITLQEIAFLGAYCyr-----ddDFA 281 uncultured bact...
YP_995569    216 RAQAPGenryDYIFDAVGArrTRQQAIAAIAPgGLVMHAGLqdga---seidMRKLTLAQITLSGTYTyt-----taDLR 287 Verminephrobact...
YP_001540012 219 RELYPEg--fDAVIDAVGStdTRRLAINTVRRgGRVVFVGLhdne---tsipGNLIVRGEIEIKGSFSyt-----deDFR 288 Caldivirga maqu...
Feature 1                                                     
YP_177063    297 AAIDYIasgkidvkpLIHDVFKLeDGDKPFqamkdpdnhsIKLLY 341 Bacillus clausii KSM-K16
YP_828728    297 ACIDMMargairvdeLITAVAPLeEGPSWFdrlyrhephlMKVIL 341 Solibacter usitatus Ellin6076
NP_866984    297 QCIDLMnrgvinvepLITAKISLaDGPKWFdrlhagdaeqMKVLV 341 Pirellula sp. 1
EDY81991     297 DALELIekgkirvdeHIAAVAPLsEGAAWFeklkn-nlvsGKVIL 340 Verrucomicrobiae bacterium DG1235
YP_003322359 293 QAVQLIedgivdvkpLIEKVVPLsQAPESFaalasggdksVKVVV 337 Thermobaculum terrenum ATCC BAA-798
YP_001430942 298 RALDLLatgwvtlddDWLEERPLaAGPAAFeellagttraAKIVL 342 Roseiflexus castenholzii DSM 13941
YP_293272    289 ATVRALeqgn-fgslEWVETRSLaDGPAAFadlaagrvaaGKVVL 332 Ralstonia eutropha JMP134
AAY82835     282 EALALLtsgk-vsgeGWAEIRPLdDGADAFididq-grapPKIIL 324 uncultured bacterium MedeBAC46A06
YP_995569    288 ATVAALhega-lgdlAWVEQRPLaQGQQAFrdmaegrsasAKIIL 331 Verminephrobacter eiseniae EF01-2
YP_001540012 289 RAVNIIesglldpreGWVDIRPLeKGQETFtelagtetkyVKIML 333 Caldivirga maquilingensis IC-167

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