Conserved Protein Domain Family
iditol_2_DH_like

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cd08235: iditol_2_DH_like 
L-iditol 2-dehydrogenase
Putative L-iditol 2-dehydrogenase based on annotation of some members in this subgroup. L-iditol 2-dehydrogenase catalyzes the NAD+-dependent conversion of L-iditol to L-sorbose in fructose and mannose metabolism. This enzyme is related to sorbitol dehydrogenase, alcohol dehydrogenase, and other medium chain dehydrogenase/reductases. The zinc-dependent alcohol dehydrogenase (ADH-Zn)-like family of proteins is a diverse group of proteins related to the first identified member, class I mammalian ADH. This group is also called the medium chain dehydrogenases/reductase family (MDR) to highlight its broad range of activities and to distinguish from the smaller short chain dehydrogenases (~ 250 amino acids vs. the ~ 350 amino acids of the MDR). The MDR proteins have 2 domains: a C-terminal NAD(P) binding-Rossmann fold domain of a beta-alpha form and an N-terminal GroES-like catalytic domain. The MDR group contains a host of activities, including the founding alcohol dehydrogenase (ADH), quinone reductase, sorbitol dehydrogenase, formaldehyde dehydrogenase, butanediol DH, ketose reductase, cinnamyl reductase, and numerous others. The zinc-dependent alcohol dehydrogenases (ADHs) catalyze the NAD(P)(H)-dependent interconversion of alcohols to aldehydes or ketones. Active site zinc has a catalytic role, while structural zinc aids in stability. ADH-like proteins typically form dimers (typically higher plants, mammals) or tetramers (yeast, bacteria), and generally have 2 tightly bound zinc atoms per subunit. The active site zinc is coordinated by a histidine, two cysteines, and a water molecule. The second zinc seems to play a structural role, affects subunit interactions, and is typically coordinated by 4 cysteines.
Statistics
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PSSM-Id: 176197
View PSSM: cd08235
Aligned: 31 rows
Threshold Bit Score: 412.374
Threshold Setting Gi: 50842108
Created: 4-Feb-2009
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
putative NAD(P)catalytic Zn
Feature 1:putative NAD(P) binding site [chemical binding site]
Evidence:
  • Comment:MDR family binds NAD(P) as a cofactor
  • Comment:defined by comparison to MDR alcohol dehydrogenase like family

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                             ###  #                                     
YP_002533911   1 MKAFVFEGVnrVALKEVSEPKlc-eeGAVIRVvSAAVCATDVKiykhghfkikdgesrILGHEIFGEIYEISDGFsdrFP 79  Thermotoga neap...
YP_429348      1 MKALVFEGPneLKLRDVPLPEpg-edEILVKVaACLICGTDLRifrgakt-rgvripsILGHEFAGVVEATGVNVk-eFH 77  Moorella thermo...
YP_831294      1 MKAARLHSPgnIRVDDIPRPSad-agDIIIRVrAASICGTDRRiaanghfklpegtprVLGHEFAGEIVEAGSEVs-gYA 78  Arthrobacter sp...
YP_548134      1 MKAAVLRAPkvLQLSDIATPEaa-pgELVLRVrAAMVCGTDLRiltgrkt-kgvrfpsVIGHEFAGEVVQVGDGVt-qFK 77  Polaromonas sp....
YP_001114581   1 MKAFLLEQAnnLVLKTIEIPRck-agEVLVKVaACGICRTDMKsyrlghr--dlhlprILGHEIAGTVVEIGAGVt-eVH 76  Desulfotomaculu...
YP_002505372   1 MLSYVYHTDktLELKDVPKPMlngegALIKTI-ACSICGTDVRthrfgst--kidegrIIGHEVVGEIIELSESVk-dFE 76  Clostridium cel...
YP_180875      1 MKAVLLEEPakLSLAEVDTPScp-kgGVLLEVaCCAICGTDVKmfrrghr--dlkyprILGHEIAAVVACSEHPD---FK 74  Dehalococcoides...
YP_055335      5 MPALVLNGPgdVQVAEKPIPTpg-pgEVVLAVeATTICGTDLRiisgekt-agvrpgvTLGHEIAGRIASLGEGVe-gLV 81  Propionibacteri...
CBH38852       1 MKAAVLSGIenLEITEVDRPAcn-sgEVLLKLeACAICKTDTKmfhsghr--dlklprILGHELAGTIIEVGKGIh-kFE 76  uncultured arch...
YP_003191063   1 MKAALLEGInkLRITNVPTPKck-qgEVLIKVgVCGMCRTDMKaymygqr--dlrlprIIGHEIAGTVVEMGEGVt-qVK 76  Desulfotomaculu...
Feature 1                                                                                #   #   
YP_002533911  80 AGTKVFVApnIGCGKCNYCLvGKYNLCpdydafgitlDGALAEYMFIParafeqgNVMKVKEnedeVFTPLVEPLSTCVY 159 Thermotoga neap...
YP_429348     78 VGDRVGVApvIPCHTCFYCKnGLENVCanrtalgyeyEGAFAEYVCIPapavkggNVYHLPSnislEEAALAEPLACCLN 157 Moorella thermo...
YP_831294     79 VGDRVSVTpnVGCGTCPNCLvGLNNMCpsyeafgitmDGGFQEYVRIPrfalnrgNVFHLPEtvgyAEAALVEPLSCCYN 158 Arthrobacter sp...
YP_548134     78 TGDRVCMDpvIPCRACAYCKtGHENVClhrqamgyefDGAFAEYIRIPaialtagNVFMMPAqmsfESAALAEPLACCIN 157 Polaromonas sp....
YP_001114581  77 CGERVQVSpgLPCGVCPNCLtGLNHLCpnieimgfhyDGGFAEYVLIPskgvkakVLNKLPDhfpiELAALTEPLACCIN 156 Desulfotomaculu...
YP_002505372  77 IGEHVAVApaIGCGICYSCKnGKTNMCedlktigfqyDGGFADYMVIPlqafkmgNVYKLPEvkddSVFTLSEPLACAIN 156 Clostridium cel...
YP_180875     75 AGDRVQVYpgIACGVCPLCLqGRENLCgqvkiigfnyDGGLAEYMALPpes-lpgGLNIIPEnvsdEEASLAEPLASCIH 153 Dehalococcoides...
YP_055335     82 VGQQATISivVSCGTCRACLaGREHLCshcelvgyglDGGLAPWLCVPaqavsrgNIIPVATeipaTRLALAEPLSCVLN 161 Propionibacteri...
CBH38852      77 VGDRVQVApgLPCGGCPFCIsGVPNMCdnmsiigfhhDGGFAEYMLVPangvqngCINTIPLslsfEAASLAEPLACCIN 156 uncultured arch...
YP_003191063  77 LGDRVQVApgLPCGSCSYCLqGLHHMCdyvqimgfhyDGGFAEYVLVPnngvscgVLNKIPEelsfQEAAITEPLACSIN 156 Desulfotomaculu...
Feature 1                         ######                  ##   #               #                 
YP_002533911 160 NLnnis-ikpGDKVLVFGAGFMGSLNALVAKHMGAsfVAVVDPkreRLEKAKEIGi-HYRLDPsvedikkvSWEITEGQG 237 Thermotoga neap...
YP_429348    158 GHhnsk-vklGDVVVILGAGPIGLMHLQLAKSSGAsyVIISEPnehRRAIAKEFGa-DRVVDPqtedlnsiVKNVTDGLG 235 Moorella thermo...
YP_831294    159 AVskld-vrpDSTVLIMGAGPIGACHVMLAKLYGArkVIVSNNrqpRLDFAGTLGa-DVLVNLterdlatvVAEETGGLG 236 Arthrobacter sp...
YP_548134    158 GQrnag-vqvGDTVVVLGAGPIGLMHAALARLSGArqVIVSEPnaaRRQAAVDRGv-EHMCDPstesvmefVKARTDGLG 235 Polaromonas sp....
YP_001114581 157 IQesmd-igsGDTIIIFGAGPVGILNAKLAKLRGAknIAIFDVnkkRLHQAAGFAf-CHLFNPleqdpvkvVRELTDGTG 234 Desulfotomaculu...
YP_002505372 157 AQsyln-ikqGEDVVIFGSGIIGCMHAELALYSGAknVIIIETsfeRIKQASKLLkdVIFINSaetdifaeVSRLTDGKG 235 Clostridium cel...
YP_180875    154 SQsvsr-vgdGDRVLVLGAGPLGLLQAMLARHNGAekVLMAEVlpdRVCGAELACp-DGVIDLaktslrqgVFEQTGGAG 231 Dehalococcoides...
YP_055335    162 GHrrhggvnpGETVVIIGGGAIGLLHTALNLACGAgrVILCEKhadRRDRAAAMG--AVTTCPehl--sevVTEHTGGYG 237 Propionibacteri...
CBH38852     157 AQelsk-iklGDTVVIIGAGPLGHLHRQLAHISGAseVMLIENspeRIAFAKKVAp-DYLIDStevdpveaVKEITDGAD 234 uncultured arch...
YP_003191063 157 MQeslq-vglGDIVIIFGAGPLGILNAKLAVARGAnkVILIEInenRLAIAKNREf-HYCVNPlqtnsikeVMEVTGGRG 234 Desulfotomaculu...
Feature 1              ## #                   ##                         ###                     
YP_002533911 238 fDVVIVTAPLpevQALSLEIVAklGRVSFFAGLPkksnfpsINTNLIHYNQLVLTGTTGADveYYRRALEMFHHRDDLre 317 Thermotoga neap...
YP_429348    236 aDIIFLAIGIpalAQDALTLVKkgGTINFFAGFSvgd-kaaLDVNLIHYNEIKITGTSAARrdDYRKALDLIAKGKVKas 314 Moorella thermo...
YP_831294    237 vDVALTCVSKpevQAQAVDLLAthGRVNFFAGLGkaq-pvaLDTNRVHYQGLTLTGTTGSSnsDYASALSLVGEGRLDls 315 Arthrobacter sp...
YP_548134    236 aDVVILAIGVpqlANESLNLVRkgGHVNLFAGFSage-tsnIDVNLIHYNEIVVTGASALRrrDYELALNMLSAGQIDan 314 Polaromonas sp....
YP_001114581 235 aEAVIPCCPDiaaFRQGLSILRkrGKFGFFSGLVgehavsvGELNNFHYKEIRLFGAYGCSseHNKKALLLLSSGMLEvd 314 Desulfotomaculu...
YP_002505372 236 aDVAIIACSVgsaQADGMKILAkcGRISLFGGLSgns-tgfIDSNLIHYREISVFGVHASTpeQNKQAMEMIHSGKINve 314 Clostridium cel...
YP_180875    232 vDVILIASSGv-eVGELPSVLSpgGRINFFSGLPkdrsefsLDANTIHYRELALSGSYGSTpaDNTRALSLITDGIIPvk 310 Dehalococcoides...
YP_055335    238 aELVIVAIGRnelTEESLNLAApgGRVSWFAGFPkgs-tttITPNTVHYQELTISGGSNASraDVHRAVDMLGRGNIDet 316 Propionibacteri...
CBH38852     235 -VVIPACSDPe-vLAQGIEMLKkrGRIVFFSGLPheyknipIDHNRIHYKELQIFGAYGCTseQNRKALKMLAARKIAvd 312 uncultured arch...
YP_003191063 235 aDVVIPCCPDpdvVGTGLAMLSkrGRFGFFSGLMmnfgsikVDFNLIHYKELRVYGAYGCSslHNRTAVEMLVTEKVKvk 314 Desulfotomaculu...
Feature 1                                         
YP_002533911 318 dikkLVTSVVTLEELPEAFERLTQGieLKVVVK 350 Thermotoga neapolitana DSM 4359
YP_429348    315 ---kMITHRFPLDKAEEAFRIAGSSqgIKVAII 344 Moorella thermoacetica ATCC 39073
YP_831294    316 ---pLISQTFTLDDIEKAMDYAGSGqgMKAMIL 345 Arthrobacter sp. FB24
YP_548134    315 ---sLITHRYEVADSLAAFDEAGSGraLKVAIH 344 Polaromonas sp. JS666
YP_001114581 315 ---nLITKIIPLEDIKEGLRMVEKLaeLKIVVK 344 Desulfotomaculum reducens MI-1
YP_002505372 315 ---kYITERYPLKDIEKAFKDIEDGrvMKAVIV 344 Clostridium cellulolyticum H10
YP_180875    311 ---rLISRVTGISDIEEAFLGVEKLkgLKTIIK 340 Dehalococcoides ethenogenes 195
YP_055335    317 ---pIVTHTFGLSQWTEAVDAVRGHvgVKIAID 346 Propionibacterium acnes KPA171202
CBH38852     313 ---eLITHHISLDELINGFELVDSHkaMEVVVT 342 uncultured archaeon
YP_003191063 315 ---dMITRCIQLEEVISGIKMIADMkeMKIVIN 344 Desulfotomaculum acetoxidans DSM 771

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