Conserved Protein Domain Family
butanediol_DH_like

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cd08233: butanediol_DH_like 
(2R,3R)-2,3-butanediol dehydrogenase
(2R,3R)-2,3-butanediol dehydrogenase, a zinc-dependent medium chain alcohol dehydrogenase, catalyzes the NAD(+)-dependent oxidation of (2R,3R)-2,3-butanediol and meso-butanediol to acetoin. BDH functions as a homodimer. NAD(P)(H)-dependent oxidoreductases are the major enzymes in the interconversion of alcohols and aldehydes, or ketones. The medium chain alcohol dehydrogenase family (MDR) have a NAD(P)(H)-binding domain in a Rossmann fold of a beta-alpha form. The N-terminal region typically has an all-beta catalytic domain. These proteins typically form dimers (typically higher plants, mammals) or tetramers (yeast, bacteria), and have 2 tightly bound zinc atoms per subunit. Sorbitol and aldose reductase are NAD(+) binding proteins of the polyol pathway, which interconverts glucose and fructose. Sorbitol dehydrogenase is tetrameric and has a single catalytic zinc per subunit.
Statistics
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PSSM-Id: 176195
View PSSM: cd08233
Aligned: 51 rows
Threshold Bit Score: 379.192
Threshold Setting Gi: 158315631
Created: 7-Jan-2009
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
putative NAD(P)catalytic Zn
Feature 1:putative NAD(P) binding site [chemical binding site]
Evidence:
  • Comment:MDR family binds NAD(P) as a cofactor
  • Comment:defined by comparison to MDR alcohol dehydrogenase like family

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                              ###  #                                    
P39714         1 MRALAYFKkGDIHFTNDIPRPeiqtDDEVIIDVsWCGICGSDLHEYLDGPIFMPkdgechklsnaaLPLAMGHEMSGIVS 80  baker's yeast
NP_979550      1 MKAAVWYGeRDIRIEERETKEl--qPNDVKVKVaWAGICGSDLHAYLHPGSVPIn-----------MNTVMGHEFSGEIA 67  Bacillus cereus...
AAQ12031       1 MDAIVLKGtNDVGLTSVPDPAp--qPGEVIIEVaATGLCGTDLHEYVAGPTFSQ------------PPVVLGHEVSGRIV 66  Mycobacterium s...
YP_951377      1 MRAAIYHGrEDVRIEELPDPSp--rAGEVVIEVaRAGICGTDLHEYIAGPMHAA------------PGVVIGHEYSGTVV 66  Mycobacterium v...
YP_951632      1 MRAAVYYGpNKLEVDDVAEPEp--gPGTVKLKVgFNGICGTDLHEYYAGPIFVPtephp--ltgqqLPLTMGHEFSGTIA 76  Mycobacterium v...
YP_001418919   1 MKAARFHArRDVRVEDIPTPTdqpaPRQVLVKNrFCGICGTDLHEYAAGPIFIPtqphp--ytgakAPQILGHEYGGTVV 78  Xanthobacter au...
YP_001508139   1 MIAARLHGpGDLRVEEVDEPEa--aAGEVKIAVaHNGLCGTDLTEIFSGPRACTtvphp--ltggvLPQIVGHEFAGVVA 76  Frankia sp. EAN...
YP_624980      1 MRALQWHGpRDVRLVEIDTPRv--gPGDVRIAVaYCGICGSDLHEYADGPHAIPvdaphp-lsrrtAPLTLGHEFCGTVV 77  Burkholderia ce...
AAG07484       1 MKALRWHAaRDLRLSELERQAp--rPGEVELEVaYCGICGSDLHEYQSGPHSIPqaeahp-lsgcrAPLTLGHEFCGVVA 77  Pseudomonas aer...
NP_355925      1 MRALRFHAaKDLRIEDIAEPKrp-gPGQVLVRNrFVGICGTDLHEYSYGPIFIPtephp--ftgahGPQILGHEFGGVVE 77  Agrobacterium t...
Feature 1                                                                                        
P39714        81 KVGPKVtKVKVGDHVVVDAasscadlhcwphskfynSKPCDACq-rGSENLCTHAGFVGLgvisGGFAEQVVVSQhhIIP 159 baker's yeast
NP_979550     68 EVGSHVtKFKKGDRVCIYPmm--------------lKDPSNAEi--ERFITLDAVGAQID----GGFAEYVILPQktIFK 127 Bacillus cereus...
AAQ12031      67 EVGAGIdRCRIGEGAAVIPmd--------------fCGSCHYCh-rSLYHLCQRPGWIGLtr-nGGLASHVAVPSrlAVR 130 Mycobacterium s...
YP_951377     67 GVGSGVrEFTEGDRVCGVGvf--------------gCGECGFCk-qGAEALCGAVGFIGFav-nGALARYASLPTkaLFR 130 Mycobacterium v...
YP_951632     77 AVGDGVtGWSEGDRVAVEPiy--------------kCGHCGPCr-aGNYNVCQQIGFHGLms-dGGMAEYTVVPTsmLHK 140 Mycobacterium v...
YP_001418919  79 AVGSSVtNVAVGDRVSVQPm---------------vSPRDDYFgqrGLYQLSESLAIIGLawpwGGMAEYSLVNDynVFR 143 Xanthobacter au...
YP_001508139  77 AVGAGVtDVAVSERVSVEPly--------------sCGDCDRCq-aSLPELCRQVMTHGIcsngGGLAQFTTVPRamVHR 141 Frankia sp. EAN...
YP_624980     78 EVGADVtTLRAGDRVAVEPey--------------rCSQCAYCr-sGSYNLCVSMGFAGLmg-dGGMADFAVVPAymLHR 141 Burkholderia ce...
AAG07484      78 ALGPGVeGPRIGDRVAVEPey--------------rCGECRYCr-eGRYNLCESMGFIGLmg-dGGFAERARVPAymLHR 141 Pseudomonas aer...
NP_355925     78 AIGDGVtSVNVGDRVSIQPlim------------prSGDYFADr--GLFHLSTQLALVGLswdgGGMAEAALVNEynVQK 143 Agrobacterium t...
Feature 1                     #   #                   ######                  ##   #             
P39714       160 VPKEiPLDVAALVEPLSVTWHAVKISGFKKGSSALVLGAGPIGLCTILVLKGMGAsKIVVSEIAeRRIEMAKK--LGVEV 237 baker's yeast
NP_979550    128 IPDHlPLELAAMVEPAAVSFQSIKDSNVREGDTVVVYGAGPIGLFAVLGAKAAGAsNIIVIDLFdSRLDKATEm-GATHV 206 Bacillus cereus...
AAQ12031     131 VPDAvDLQEAALTEPTAVAFHAVRRAELLLGETVLVLGAGALGLTVIQCARAAGAaRIYVTEPSrVRGNLARDl-GATLV 209 Mycobacterium s...
YP_951377    131 IPDEiSLAEAAVVEPIASAYHAVRRSGLAAGGTVFIAGAGPIGLALVQFSLAQGAtQVIVSEVSaTRRVAAHRv-GATRV 209 Mycobacterium v...
YP_951632    141 LPDNvSLELGALVEPMSVAYHAATLGDVSPGDTAMVFGAGPIGIGLWFALRGKGLdDVFVVEPSpTRRAAIEV--LGART 218 Mycobacterium v...
YP_001418919 144 VPDSvSDAQAALIEPTAVTVYAAERGGVKPGDSVLVTGAGPIGQLQVLAARAAGAtRIFLSDTNdNRLAMARQilPDVIT 223 Xanthobacter au...
YP_001508139 142 LPASmSLAEGALVEPMAVAFNGVLRSGVEAGGSALVFGAGPIGMGVVFGLRAVGVaDILLVEPAsARRAAAERl-GVADV 220 Frankia sp. EAN...
YP_624980    142 LPDGvSLEQAAVMEPAAVALHALRRGELRLGETCAVFGLGPIGLLLIMLAKLQGAtTIVAVDVSpERLAAATRf-GATHA 220 Burkholderia ce...
AAG07484     142 LPDAvGFRQAAVLEPAAVALHALRRSSLAPGQRCAVFGLGPIGLLLVMLARLRGIeDIAAVDVSpERLALAGEf-GASRA 220 Pseudomonas aer...
NP_355925    144 IPDEmTDEEAALVEPSAVAVYACDRGGVTAGNSVLVTGAGPIGMLTLLAARAAGAtQLFVSDLNdARLELARNviRDVIT 223 Agrobacterium t...
Feature 1          #                           ## #                      ##                      
P39714       238 FNPskhgh-ksieiLRGLTKShdgfDYSYDCSGIQvTFETSLKALTfKGTATNIAVWGp-KPVPFQpm-dVTLQEKVMTG 314 baker's yeast
NP_979550    207 FNAkevn---pveeIRKLFPGg--aDVAIEAAGVEsTFNQAIQSTKvRGTMMVISFHT--QDIQFNvpasLIFSGAKLMG 279 Bacillus cereus...
AAQ12031     210 LDPhepg---ttarILEETRGvg-vDVVFHVAGSVeAFTQGLDCLRkQGRFMEMSSWA--GPASLDvn-rHLLKETQLRM 282 Mycobacterium s...
YP_951377    210 IDPlaed---avevVRMLTNGng-vDISFDAAGVQpALDAALGVLRpRGRLMVVAIWE--APAGIDin-rSVMREANIGF 282 Mycobacterium v...
YP_951632    219 LDPtsad---vpafIADHTRGdg-aDAVFDAAGVTpAVSTALACVGsRKPMISVAIYE--KPLETPll-nLVMNESRIQG 291 Mycobacterium v...
YP_001418919 224 LNPrsdk---vpeaIRDQTEGgvgvDVALECVGAEaALGTCIEAVRrQGVVVQVGLHVg-KPAVDGf--tVTFKDIDVRG 297 Xanthobacter au...
YP_001508139 221 LDPnttd---vadeVRRRTGGrg-vDVVLDAAGAPgTFGLAPAVLRaRGRYVGIAVAE--RPVEFApw-vLARGEIELTG 293 Frankia sp. EAN...
YP_624980    221 FDArtldaaalqdaIRAATGGlg-vDTSFEAAGLQaTFESAMRALRkGGRVVMVGLMPhaGFDAFR----AVNDELTVTT 295 Burkholderia ce...
AAG07484     221 LDArdg------dtAARLREGga-lDCAFEAAGSQaSLDAALASLRkGGELVLVSLMGevRLDAFD----LVNRELRLLG 289 Pseudomonas aer...
NP_355925    224 INPkrdn---vgdvVRSATEGkvgcDVAIECVGNEhALKACVDAVRkQGVVVQTGLHPheNPIDWFq---VTFRDLEIKG 297 Agrobacterium t...
Feature 1        ###                                                          
P39714       315 SIGYv-vEAFEEVVRAIHNGdiamedcKQLITGKQRIEdGWEKGFQELMDHKEsnVKILLT 374 baker's yeast
NP_979550    280 SVGYs-nETYNEVIELFANGrl---paQSIITSKIDIDnIAEQGFEALIHDKSq-AKILVK 335 Bacillus cereus ATCC 10987
AAQ12031     283 VFGYdmfDDFPAVLALMADGki---alTPQITTRIPLDsAVEEGLNGLLEAREglIKVLVR 340 Mycobacterium sp. S65
YP_951377    283 SFCYeaqRQVPAILDLLATGai---nlGELITDEIPLDaVVSQGLEELRVNRDahVKILID 340 Mycobacterium vanbaalenii PYR-1
YP_951632    292 SLCYt-gADFEAVIALMGQGky----dTTGWVTRIPIDdVVDEGFEALHAGTK--MKVLVD 345 Mycobacterium vanbaalenii PYR-1
YP_001418919 298 SWCYt-tLMWPRVAALIGSGil---paEKVVTRRIRLDeVVENGFEALLSPAGteLKILID 354 Xanthobacter autotrophicus Py2
YP_001508139 294 SLGYg-pGVFTRVIDLIAGGay----pTAGWVDHVGLS-DLHDALQDLRDGRR--MKVLVD 346 Frankia sp. EAN1pec
YP_624980    296 SVGYr--HAYEDLLRIVASGal---dlTSIVTRTVSLEdAVADGFDALLADRAq-IKILVS 350 Burkholderia cenocepacia AU 1054
AAG07484     290 SVGYr--DAYPELIALLADGrl---dlARAVTRSVPLEqAVEHGFEALLRDKSq-LKVLVN 344 Pseudomonas aeruginosa PAO1
NP_355925    298 SWAYp-tHYWPRVIRLIASGll---paTKIVTKRITLDtAVTEGFDALLDPAGthLKILID 354 Agrobacterium tumefaciens str. C58

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