Conserved Protein Domain Family
MPN_NLPC_P60

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cd08073: MPN_NLPC_P60 
Mpr1p, Pad1p N-terminal (MPN) domains with catalytic isopeptidase activity (metal-binding) found in proteins also containing NlpC/P60 domains
This family contains bacterial MPN (also known as Mov34, PAD-1, JAMM, JAB, MPN+)-like domains at the N-terminus of NlpC/P60 phage tail protein domains. These domains contain the signature JAB1/MPN/Mov34 metalloenzyme (JAMM) motif, EXnHS/THX7SXXD, which is involved in zinc ion coordination and provides the active site for isopeptidase activity for the release of ubiquitin from ubiquitinated proteins (thus having deubiquitinating (DUB) activity) that are tagged for degradation. The JAMM proteins likely hydrolyze ubiquitin conjugates in a manner similar to thermolysin, in which the zinc-polarized aqua ligand serves as the nucleophile, compared with the classical DUBs that do so with a cysteine residue in the active site.
Statistics
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PSSM-Id: 163704
View PSSM: cd08073
Aligned: 35 rows
Threshold Bit Score: 95.4415
Threshold Setting Gi: 220926764
Created: 7-Oct-2009
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
MPN+ (JAMM)Zinc-binding
Feature 1:MPN+ (JAMM) motif
Evidence:
  • Comment:Based on literature and mutation studies

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                            #                                                 # #       
NP_287597     49 QTESAILVHAr----RCAPAESCGFVIGtp-egERYQPCVNISAepeayFRIAPEDWLQAEmqgeIVALVHSHPGg---- 119 Escherichia col...
YP_001685122   1 MIPQEVWEQIkpifaLAYPCEAVVAVMPd----LTWRHLENTAAdplrgFDISQADMADLMee-pPLVLLHSHCSg---- 71  Caulobacter sp....
ZP_03719880    7 EIQDLIFWVAd----GYAPQEMCGAIADihdkgRRFFLIDNIAEnpeetFEMNPKGWQALAadgeIVAVVHSHPNg---- 78  Neisseria flave...
ZP_02960019    1 MLEKECIEYA-----NESDLEQCGLIIDn----SHLFKCKNSHHdprkhFRISGDEWSEAEklgeITAIIHSHPKt---- 67  Providencia stu...
CBA05329      10 EARREMLACAe----EAVPSEMCGVLVFsy-egYEFLPLSNCAEnphetFEISADDWMAAErvgeIVAVVHSHPRg---- 80  Neisseria menin...
ZP_05729337    2 TLEDECLLYS-----ASSGNEVCGLILNn----THLVRCENVHPqpsqhFRISDEDWIKAEtageITAVFHSHPNp---- 68  Pantoea sp. At-9b
YP_003007011   6 ELKKEILDYAk----KQEPREMCGFVVLed-geKVFYPCPNISSdpenyFEIDPEEWIMTSeigeIIALVHSHPNg---- 76  Aggregatibacter...
YP_002475192   2 TLEEKIIAHAk----RSEPHESCGFVVSkg-geLRYFPCENVAVdpinhFEISPDDWIRAEsvgeIVAVVHSHPDsdtek 76  Haemophilus par...
ZP_00134777    5 HIKIEILAHAk----KSEPQESCGFVVSgq-deFFYYPCENVADdpesfFEIAPEAYIQAEylgeIVAIVHSHPNg---- 75  Actinobacillus ...
ZP_04978012    2 ELEQQIIDYAl----QHEPHEMCGFVVFdg-nkNRFIPCENQAEdkanyFEISDLDYIKAEekgeLVAVVHSHPEpn--- 73  Mannheimia haem...
Feature 1             #  #                                 
NP_287597    120 -lPWLSEADRRLQIKSAL-PWWLVCr-----GEIHKFRCVPH 154 Escherichia coli O157:H7 EDL933
YP_001685122  72 -iADPSDLDTLQQMATGW-TWGIVAvtgnlqGQVYNVAYPEV 111 Caulobacter sp. K31
ZP_03719880   79 -ePFLSGADRQMQIQTGL-AWILAVg-----GRLKQFRCCPH 113 Neisseria flavescens NRL30031/H210
ZP_02960019   68 -rLALSAGDRKQQLMTGL-EWWLVSn-----NRLRIFKPVPH 102 Providencia stuartii ATCC 25827
CBA05329      81 -ePFLSGADRRMQVETGL-PWILVTq-----GRLKLFRPVPH 115 Neisseria meningitidis
ZP_05729337   69 -kLVLSPADRTAQIATGI-DWWLASa-----GLLRKIRPVPH 103 Pantoea sp. At-9b
YP_003007011  77 -lPYLSTADRMCQIRCATaFWLVVD------NEILQFRPAPA 111 Aggregatibacter aphrophilus NJ8700
YP_002475192  77 gfPYLSTADRECQVRLNL-PFWLVCd-----GNLQKFRPIAP 112 Haemophilus parasuis SH0165
ZP_00134777   76 -ePALSIADRQMQDLSQL-DWWLVCn-----GELHIFPKIQP 110 Actinobacillus pleuropneumoniae serovar 1 str. 4074
ZP_04978012   74 gkPILSVLDRKMQVQTGL-DWWLVHn-----RQIHKFRNVPH 109 Mannheimia haemolytica PHL213

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